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The map label for this gene is pcnB1 [H]

Identifier: 124028141

GI number: 124028141

Start: 1290312

End: 1291073

Strand: Direct

Name: pcnB1 [H]

Synonym: Hbut_1286

Alternate gene names: 124028141

Gene position: 1290312-1291073 (Clockwise)

Preceding gene: 124028140

Following gene: 124028142

Centisome position: 77.4

GC content: 44.88

Gene sequence:

>762_bases
ATGGGGTTTAGAGCCGTCTACCCCGCAGCAACAAAGTTTAAGTATATAATCCAGACTATTGCAAAGGTTATGGATGAAAT
ACCATTCATTGTTGCGCAAGACGGTCTAGACGTAAGAACGCTAACACCTGACAAGACGACAATGATTATACTAAGGCTTC
CCGTTACAGCCTTCGAAGAGTACCAGTTAGACGAGGATAAGAAGACATTCATAGTGCTAGCTGACGAGCTTAACAAGGTT
GCTAAGCGTGGTACGCGAAATGATATTGTTGAGCTGAAGCTTGACGAGGAGCATCGTAGACTTGAGGTTAACTTCATAGA
TAAAAAGACTAATGTTGTACGTAGCTTCTATGTATCTCTTCGTGAGGGTACTGTTGAAGAGCTTGCTGAACCGCAGGTGG
AGCTAACGGTTACAGCGAAAATGATGGCTGACGACTTTAAGAATGTTATAAATGATGCAAAGATAGTAAGCGACGAAGTC
GAGTTTGCGGCGTACGAGGATAGAATAGAAGTTTATGCTGAGTCGGCTCAGAAGAAGTATATGGGTGTACTCAAGGTTGG
CATGCCACTTATAACGTTAAATGTTGAAGGCGGTACCCCGATACGTGCTAAGTACTCGATAGACTTGCTGAAAGCGGCTG
TTAAGGCAACAACGGCTGCTAATACCGTAACACTGGAATATGGTGAGGCATTGCCGATGAGACTAAGCTTTGACCTGCCC
GGCGGCGGGCTGTTAATATACTGGATATCGCCCAGAGTCTAG

Upstream 100 bases:

>100_bases
AGTATAGTCTAAGTATGCGGGTTTTTAGCGCTACATTACTTCTTTTACCCTAACATGTTAAGAGGGATTCCAGAGCTGAG
TAATAACCCTGGGGAGCTAT

Downstream 100 bases:

>100_bases
CCTCCCTCAAGCTTTCTCAGAAGTTCCTCTAAGGCCCTCCTAGCTTCCTCAAGCTTAGGCTGGGGCTTGGCTGCAGGCTG
TACATGTCTAAGCGTGGGGA

Product: DNA polymerase sliding clamp subunit

Products: NA

Alternate protein names: Proliferating cell nuclear antigen homolog B1; PCNA B1 [H]

Number of amino acids: Translated: 253; Mature: 252

Protein sequence:

>253_residues
MGFRAVYPAATKFKYIIQTIAKVMDEIPFIVAQDGLDVRTLTPDKTTMIILRLPVTAFEEYQLDEDKKTFIVLADELNKV
AKRGTRNDIVELKLDEEHRRLEVNFIDKKTNVVRSFYVSLREGTVEELAEPQVELTVTAKMMADDFKNVINDAKIVSDEV
EFAAYEDRIEVYAESAQKKYMGVLKVGMPLITLNVEGGTPIRAKYSIDLLKAAVKATTAANTVTLEYGEALPMRLSFDLP
GGGLLIYWISPRV

Sequences:

>Translated_253_residues
MGFRAVYPAATKFKYIIQTIAKVMDEIPFIVAQDGLDVRTLTPDKTTMIILRLPVTAFEEYQLDEDKKTFIVLADELNKV
AKRGTRNDIVELKLDEEHRRLEVNFIDKKTNVVRSFYVSLREGTVEELAEPQVELTVTAKMMADDFKNVINDAKIVSDEV
EFAAYEDRIEVYAESAQKKYMGVLKVGMPLITLNVEGGTPIRAKYSIDLLKAAVKATTAANTVTLEYGEALPMRLSFDLP
GGGLLIYWISPRV
>Mature_252_residues
GFRAVYPAATKFKYIIQTIAKVMDEIPFIVAQDGLDVRTLTPDKTTMIILRLPVTAFEEYQLDEDKKTFIVLADELNKVA
KRGTRNDIVELKLDEEHRRLEVNFIDKKTNVVRSFYVSLREGTVEELAEPQVELTVTAKMMADDFKNVINDAKIVSDEVE
FAAYEDRIEVYAESAQKKYMGVLKVGMPLITLNVEGGTPIRAKYSIDLLKAAVKATTAANTVTLEYGEALPMRLSFDLPG
GGLLIYWISPRV

Specific function: Sliding clamp subunit. Responsible for tethering the catalytic subunit of DNA polymerase to DNA during high-speed replication [H]

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PCNA family [H]

Homologues:

Organism=Homo sapiens, GI33239451, Length=255, Percent_Identity=19.6078431372549, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI4505641, Length=255, Percent_Identity=19.6078431372549, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000730
- InterPro:   IPR022659
- InterPro:   IPR022648 [H]

Pfam domain/function: PF00705 PCNA_N [H]

EC number: NA

Molecular weight: Translated: 28491; Mature: 28360

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFRAVYPAATKFKYIIQTIAKVMDEIPFIVAQDGLDVRTLTPDKTTMIILRLPVTAFEE
CCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEECCCCEEEEECCCCEEEEEEEECCHHHHH
YQLDEDKKTFIVLADELNKVAKRGTRNDIVELKLDEEHRRLEVNFIDKKTNVVRSFYVSL
CCCCCCCCEEEEEHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEEECCHHHHHHHHHHHH
REGTVEELAEPQVELTVTAKMMADDFKNVINDAKIVSDEVEFAAYEDRIEVYAESAQKKY
HCCCHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MGVLKVGMPLITLNVEGGTPIRAKYSIDLLKAAVKATTAANTVTLEYGEALPMRLSFDLP
HHHHCCCCEEEEEEECCCCCEEEEHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEECC
GGGLLIYWISPRV
CCCEEEEEECCCC
>Mature Secondary Structure 
GFRAVYPAATKFKYIIQTIAKVMDEIPFIVAQDGLDVRTLTPDKTTMIILRLPVTAFEE
CCCCCCCCHHHHHHHHHHHHHHHHHCCEEEECCCCEEEEECCCCEEEEEEEECCHHHHH
YQLDEDKKTFIVLADELNKVAKRGTRNDIVELKLDEEHRRLEVNFIDKKTNVVRSFYVSL
CCCCCCCCEEEEEHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEEECCHHHHHHHHHHHH
REGTVEELAEPQVELTVTAKMMADDFKNVINDAKIVSDEVEFAAYEDRIEVYAESAQKKY
HCCCHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MGVLKVGMPLITLNVEGGTPIRAKYSIDLLKAAVKATTAANTVTLEYGEALPMRLSFDLP
HHHHCCCCEEEEEEECCCCCEEEEHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEECC
GGGLLIYWISPRV
CCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]