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The map label for this gene is frvX [C]

Identifier: 124028016

GI number: 124028016

Start: 1164473

End: 1165597

Strand: Direct

Name: frvX [C]

Synonym: Hbut_1154

Alternate gene names: 124028016

Gene position: 1164473-1165597 (Clockwise)

Preceding gene: 124028013

Following gene: 124028017

Centisome position: 69.85

GC content: 55.56

Gene sequence:

>1125_bases
TTGACTCTACCTATCAGCGAGGGCGAGCTGGACGAGTTCTTCAAGCTACTCAAAAAACTCTCAGACGCCTTTGGCGTCTC
GGGCTACGAGGACGAAGTTAGAGGGATAATTATAGACGAGGTACGCGAGGCTGCCGACCATGTGGAAGTTGACCGCTTCG
GCAATGTTATCGCCATAAAACGTGGAGGCCGCGGCACCATCAAAATCGTCTGGGACGCTCACATGGACGAGATAGGGTTC
CTAGTCAAACACATAGATGATAAAGGCTTTATCTATCTCTCACCAGTCGGCGGCTGGACCGACCACGTGCTGCCCGGGCA
GAGGGTGCGAATACTCACAGACAACGGTACTTTAGTACGAGGCGTGATAGGGATAAAGCCACCACACCTCATGAAGCAGG
AAGAGCGCAGCCAGGTGATACCCCTCGACAAACTCTTCGTAGACATCGGTGCTTCGAGCAGGGAGGAGGTTGAGAAGCTC
GGTATAAGGATTGGAAGCCCTGTAGACCTTGACCGAGAGACGATACGGCTGGTTGGCGACAGGGTAACCGGCAAGGCCTT
TGACGACCGTGTCGGCGTAGCTGTGCTCATAAAGGCGTTCAAGGAGTTTAACCCTGCTGAGCAGACCGTATACCTTGTCA
TAGCTACGCAGGAGGAGGTAGGCCTTAAGGGCGCACGTGTGGCAGCTCAGAAGATACAGCCAGAGGCAGCAATAGCCGTA
GACGTCACAACCGCCAATGACGTACCAGGTGTAGAGGCAAAGGACCGCGTGGCAGAGGTAGGTAAGGGGCCCGCATTGAC
CGTTGCCGATGGGAGGAATGCTAGCGGGCTCATAGCGCATCCCAAACTGCTAAAGCTGCTTATGGAAACAGCTAAGGCTG
AGGGGATACCCTACCAACTCTTCATCCTACCTGGAGGTACCACCGACGCCACCGCCATAGCGCTAGCAGGGGAGGGTGTA
CCCTCAGCCGTGGTATCGGTGCCAACACGTTACATCCACAGCCCTGTCGAGCTGCTAAGCCTCCGCGACGCAGTATACGC
CGCGAAGCTTGTCGCAGCGGCTACCTCGAGGATAACCAGGGAGTGGTACGACCGCGAAATACTGTGGGGCAAGAAGATAA
AGTAG

Upstream 100 bases:

>100_bases
CCAGGACAGCCGCCACATAACCCTACCAGAGATACTATTTTCACGCAGCAACAACAAGCAAAACCACGGCCCACACAGCC
CCACCGGAGGGGTGTCCGCT

Downstream 100 bases:

>100_bases
CTTGTAACCCCAACTTTTAACCCTGCCACACCACAATCCTTCCGGACAACCTTATTCACGTAGCTCAGCGTCCTTTGGTG
GCGTGCTGTGAGCCTCGAGG

Product: endoglucanase M

Products: D-glucose; GDP

Alternate protein names: NA

Number of amino acids: Translated: 374; Mature: 373

Protein sequence:

>374_residues
MTLPISEGELDEFFKLLKKLSDAFGVSGYEDEVRGIIIDEVREAADHVEVDRFGNVIAIKRGGRGTIKIVWDAHMDEIGF
LVKHIDDKGFIYLSPVGGWTDHVLPGQRVRILTDNGTLVRGVIGIKPPHLMKQEERSQVIPLDKLFVDIGASSREEVEKL
GIRIGSPVDLDRETIRLVGDRVTGKAFDDRVGVAVLIKAFKEFNPAEQTVYLVIATQEEVGLKGARVAAQKIQPEAAIAV
DVTTANDVPGVEAKDRVAEVGKGPALTVADGRNASGLIAHPKLLKLLMETAKAEGIPYQLFILPGGTTDATAIALAGEGV
PSAVVSVPTRYIHSPVELLSLRDAVYAAKLVAAATSRITREWYDREILWGKKIK

Sequences:

>Translated_374_residues
MTLPISEGELDEFFKLLKKLSDAFGVSGYEDEVRGIIIDEVREAADHVEVDRFGNVIAIKRGGRGTIKIVWDAHMDEIGF
LVKHIDDKGFIYLSPVGGWTDHVLPGQRVRILTDNGTLVRGVIGIKPPHLMKQEERSQVIPLDKLFVDIGASSREEVEKL
GIRIGSPVDLDRETIRLVGDRVTGKAFDDRVGVAVLIKAFKEFNPAEQTVYLVIATQEEVGLKGARVAAQKIQPEAAIAV
DVTTANDVPGVEAKDRVAEVGKGPALTVADGRNASGLIAHPKLLKLLMETAKAEGIPYQLFILPGGTTDATAIALAGEGV
PSAVVSVPTRYIHSPVELLSLRDAVYAAKLVAAATSRITREWYDREILWGKKIK
>Mature_373_residues
TLPISEGELDEFFKLLKKLSDAFGVSGYEDEVRGIIIDEVREAADHVEVDRFGNVIAIKRGGRGTIKIVWDAHMDEIGFL
VKHIDDKGFIYLSPVGGWTDHVLPGQRVRILTDNGTLVRGVIGIKPPHLMKQEERSQVIPLDKLFVDIGASSREEVEKLG
IRIGSPVDLDRETIRLVGDRVTGKAFDDRVGVAVLIKAFKEFNPAEQTVYLVIATQEEVGLKGARVAAQKIQPEAAIAVD
VTTANDVPGVEAKDRVAEVGKGPALTVADGRNASGLIAHPKLLKLLMETAKAEGIPYQLFILPGGTTDATAIALAGEGVP
SAVVSVPTRYIHSPVELLSLRDAVYAAKLVAAATSRITREWYDREILWGKKIK

Specific function: Unknown

COG id: COG1363

COG function: function code G; Cellulase M and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M42 family [H]

Homologues:

Organism=Escherichia coli, GI1790332, Length=347, Percent_Identity=34.8703170028818, Blast_Score=225, Evalue=3e-60,
Organism=Escherichia coli, GI87082417, Length=351, Percent_Identity=35.3276353276353, Blast_Score=202, Evalue=2e-53,
Organism=Escherichia coli, GI1788727, Length=351, Percent_Identity=34.7578347578348, Blast_Score=170, Evalue=1e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008007 [H]

Pfam domain/function: PF05343 Peptidase_M42 [H]

EC number: 3.2.1.4

Molecular weight: Translated: 40634; Mature: 40503

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLPISEGELDEFFKLLKKLSDAFGVSGYEDEVRGIIIDEVREAADHVEVDRFGNVIAIK
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHCEEEHHHHHHHHHHCCHHHCCCEEEEE
RGGRGTIKIVWDAHMDEIGFLVKHIDDKGFIYLSPVGGWTDHVLPGQRVRILTDNGTLVR
ECCCEEEEEEEECCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCEEEEEECCCCEEE
GVIGIKPPHLMKQEERSQVIPLDKLFVDIGASSREEVEKLGIRIGSPVDLDRETIRLVGD
EEECCCCCHHHCHHHHCCCCCHHHHHEECCCCCHHHHHHHCCCCCCCCCCCHHHHHHHCC
RVTGKAFDDRVGVAVLIKAFKEFNPAEQTVYLVIATQEEVGLKGARVAAQKIQPEAAIAV
CCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEECHHHCCCHHHHHHHHCCCCEEEEE
DVTTANDVPGVEAKDRVAEVGKGPALTVADGRNASGLIAHPKLLKLLMETAKAEGIPYQL
EEEECCCCCCCCHHHHHHHCCCCCEEEEECCCCCCCEEECHHHHHHHHHHHHCCCCCEEE
FILPGGTTDATAIALAGEGVPSAVVSVPTRYIHSPVELLSLRDAVYAAKLVAAATSRITR
EEEECCCCCCEEEEEECCCCCHHHHHHCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
EWYDREILWGKKIK
HHHCCCCCCCCCCC
>Mature Secondary Structure 
TLPISEGELDEFFKLLKKLSDAFGVSGYEDEVRGIIIDEVREAADHVEVDRFGNVIAIK
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHCEEEHHHHHHHHHHCCHHHCCCEEEEE
RGGRGTIKIVWDAHMDEIGFLVKHIDDKGFIYLSPVGGWTDHVLPGQRVRILTDNGTLVR
ECCCEEEEEEEECCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCEEEEEECCCCEEE
GVIGIKPPHLMKQEERSQVIPLDKLFVDIGASSREEVEKLGIRIGSPVDLDRETIRLVGD
EEECCCCCHHHCHHHHCCCCCHHHHHEECCCCCHHHHHHHCCCCCCCCCCCHHHHHHHCC
RVTGKAFDDRVGVAVLIKAFKEFNPAEQTVYLVIATQEEVGLKGARVAAQKIQPEAAIAV
CCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEECHHHCCCHHHHHHHHCCCCEEEEE
DVTTANDVPGVEAKDRVAEVGKGPALTVADGRNASGLIAHPKLLKLLMETAKAEGIPYQL
EEEECCCCCCCCHHHHHHHCCCCCEEEEECCCCCCCEEECHHHHHHHHHHHHCCCCCEEE
FILPGGTTDATAIALAGEGVPSAVVSVPTRYIHSPVELLSLRDAVYAAKLVAAATSRITR
EEEECCCCCCEEEEEECCCCCHHHHHHCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
EWYDREILWGKKIK
HHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GDPglucose; H2O

Specific reaction: GDP-glucose + H2O = D-glucose + GDP

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]