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The map label for this gene is ypfI [C]

Identifier: 124028015

GI number: 124028015

Start: 1162157

End: 1164388

Strand: Reverse

Name: ypfI [C]

Synonym: Hbut_1153

Alternate gene names: 124028015

Gene position: 1164388-1162157 (Counterclockwise)

Preceding gene: 124028018

Following gene: 124028014

Centisome position: 69.84

GC content: 57.71

Gene sequence:

>2232_bases
GTGGCGGCTGTCCTGGGGCGGCTAGACTTGTACATGGTGCTACGCCAGCTATCAGCCTGGCTTACAGAGCTGAGAGGGAG
CGGCTACCGCGGACTCGTACTACTTCACTCAACAGCTATCGGAGTCTGTGCTAGCGCTGTGGCGGGGCTGCTCAGCGAGG
TCTATAGCCAGGTTGCTTGTATTGCGCCAGCCGAGCTGAGGAGAGAGCTCCAGGAGCGGTGTAGCCGCGTACTCAGCCCT
AGTGCTATCGAGGAGCTGTTAGGAACGGAGAACGATGCTGTACTCCTAGCTGTTCCAGGCCTGCTTAGACCCAACCTGCT
AGCAGCAGCCGCTGAAACCGTGAGGGGCGGTGGCGTTGTAGTCCTCGTTGTACCCAGGCTTGACGTGTGGCAGCCCGGCG
GTGGAATGAGCACTGGGCTCTACCGCGAATACCTCCTCCGCAGTCTCGGCAGGCTGGAGAGCCTCTTCTGGGCAGATGCA
GACCTTGACACGGTCTATTCCATGAGGCTCCCTCGCGGCAAGGCCAGAAAGCCACCAGGCCCCGAGGGCTACCGTGCCAG
GAGCCCAGTACACCGCAAGCTTCTCGAAGCGGCTGCTACGATAGAGCAGGCAGAGGCCCTCGACAAGATAGTAGCCCACG
TGAGGTCGAGGGGTAGGAGCATCCTAGTACTGGGCGACCGGGGACGGGGCAAGTCTGGCCTCCTAGGCCTGGCGGTAGCC
TACATAGTTGCAAGCCACATGGCTGGCTTTGTATCCGTGACAGCGCCGAGTCCAGCAAATGTTCAAAGCTTCTTCCGCAT
ACTCAGACTCGCGCTTGGAAAGCTGGGTACCAGGCACTGGGAGATAAGGCGTGGAAACATTGTAATCGGTATTGCCGGGC
CATGGTTTCACGTGAGATACCATACCCCAGACCGTGCCGATCCGGGCTCCTTCACCGTGGTTGATGAGGCGGCTGCACTA
GGCCCCTCAAGGCTTAGGAGTATTGCTAGACGGGCTCCCAGGCTCCTAGCAGCTACAACCATACACGGCTACGAGGGTAG
CGGCCGCGTGCTAGCCAAGATTGCGGAGGAGATACTCCCTGAGCCAAGACTCGTAGTGGAGCTCCGAACACCGGTCCGCT
ACCCACCCGGGGATCCCCTAGAGGGCTGGCTCTACGACACCTTCATGCTTCGGGTCTCGGAGCCTGAACCGCCAAGCCTT
GACGGTGTAGAACTGCGTCCAGCAAAGCTCGACCGCAAGGAGCTGGTAGAGAAGCAGCAGCTCCTCAGGGCAGTCTATTC
AATCCTTGTATCGGCACATTATCGCAACGAGCCAGACGACCTAGCACTACTCCTCGACGCTCCACACCACCGCGTATACG
TTCTCCTAGCGGGGAATACTCCTGTCGCGGTAGCCCATGCAGCCTTGGAGGGTCCCGAGCTGCCTGTAACGGCTAGGATA
CTGCCAGACATACTCGCCCAGTACGCACCCGAAGCAGGCAAGCTCAGCGGCGTGAGGATCGTAAGGATCGCTGTGCATAG
CCGGCTCCAGCGCCGCGGCCTGGGCAGCAAGCTTCTACGCTATGTGGAGGAGGTGGCATCTAGCGATGGATACGACTGGG
TTGGAGCAGTTTTTGGTAGGCCGGACGTACTCGGCTTCTGGCTCGCAAACAACTACACTCCGATCTACGTGTCGCCATTG
CCCAACAAGGTTACGGGTGAGCATAACATAGCAGTCGTGAAGCCCATAGCTAACAAGGCCGCAAAGATACTAGGAGGCGT
CGCAGCGGCTGCGAGGAAGAGAATGCTTCTAGCATCGCATAGCCTCTACCGTGGCCTCCCCGCAGAAATATTAGTAGAAA
TATTCTCCCACCAGGTGCCAGCGCAGCAGCCCCTATCGGATATAGATGAGTGGCAAAGGTATAGGCTCAGCTTATTCCTA
TGCGGTAGCCTTGAAGCAGAATCTGTGCTCGACGTGCTATGGCTACTACTCGTAAACGATATACTGGCAAGAGGTAGGCT
GGACCTTGAAGACCCTAAAGCGGAACTGGTGGCGGTTATGAGGATACTGCAGGGTAAAACCCTTGCAGACATAACAGCAG
CCATAAGGGTCGATAGTAGTGAGGTGCGGAAGCATCTTAGAAGCCTAGCGCTACTACTAGCCAAGCGGGCTGGTATAGAA
GCTGGTGACAGTTGTGAAGCAGCCTCAGAGCCTAGTAGTATTAGCACTAATCCAAGGCTTGCTACTAGCTAG

Upstream 100 bases:

>100_bases
TGATAGGTAGAGTCAAAGCGGACACCCCTCCGGTGGGGCTGTGTGGGCCGTGGTTTTGCTTGTTGTTGCTGCGTGAAAAT
AGTATCTCTGGTAGGGTTAT

Downstream 100 bases:

>100_bases
CTTCCCAGCAGGGGCAACAAGGCTAGAAGACTGCAATTGTACTGTCAACATGGTTTCGAGTGAACCCATCCAATCCATGC
CAGGTGTTTACATTGTGCAG

Product: P-loop ATPase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 743; Mature: 742

Protein sequence:

>743_residues
MAAVLGRLDLYMVLRQLSAWLTELRGSGYRGLVLLHSTAIGVCASAVAGLLSEVYSQVACIAPAELRRELQERCSRVLSP
SAIEELLGTENDAVLLAVPGLLRPNLLAAAAETVRGGGVVVLVVPRLDVWQPGGGMSTGLYREYLLRSLGRLESLFWADA
DLDTVYSMRLPRGKARKPPGPEGYRARSPVHRKLLEAAATIEQAEALDKIVAHVRSRGRSILVLGDRGRGKSGLLGLAVA
YIVASHMAGFVSVTAPSPANVQSFFRILRLALGKLGTRHWEIRRGNIVIGIAGPWFHVRYHTPDRADPGSFTVVDEAAAL
GPSRLRSIARRAPRLLAATTIHGYEGSGRVLAKIAEEILPEPRLVVELRTPVRYPPGDPLEGWLYDTFMLRVSEPEPPSL
DGVELRPAKLDRKELVEKQQLLRAVYSILVSAHYRNEPDDLALLLDAPHHRVYVLLAGNTPVAVAHAALEGPELPVTARI
LPDILAQYAPEAGKLSGVRIVRIAVHSRLQRRGLGSKLLRYVEEVASSDGYDWVGAVFGRPDVLGFWLANNYTPIYVSPL
PNKVTGEHNIAVVKPIANKAAKILGGVAAAARKRMLLASHSLYRGLPAEILVEIFSHQVPAQQPLSDIDEWQRYRLSLFL
CGSLEAESVLDVLWLLLVNDILARGRLDLEDPKAELVAVMRILQGKTLADITAAIRVDSSEVRKHLRSLALLLAKRAGIE
AGDSCEAASEPSSISTNPRLATS

Sequences:

>Translated_743_residues
MAAVLGRLDLYMVLRQLSAWLTELRGSGYRGLVLLHSTAIGVCASAVAGLLSEVYSQVACIAPAELRRELQERCSRVLSP
SAIEELLGTENDAVLLAVPGLLRPNLLAAAAETVRGGGVVVLVVPRLDVWQPGGGMSTGLYREYLLRSLGRLESLFWADA
DLDTVYSMRLPRGKARKPPGPEGYRARSPVHRKLLEAAATIEQAEALDKIVAHVRSRGRSILVLGDRGRGKSGLLGLAVA
YIVASHMAGFVSVTAPSPANVQSFFRILRLALGKLGTRHWEIRRGNIVIGIAGPWFHVRYHTPDRADPGSFTVVDEAAAL
GPSRLRSIARRAPRLLAATTIHGYEGSGRVLAKIAEEILPEPRLVVELRTPVRYPPGDPLEGWLYDTFMLRVSEPEPPSL
DGVELRPAKLDRKELVEKQQLLRAVYSILVSAHYRNEPDDLALLLDAPHHRVYVLLAGNTPVAVAHAALEGPELPVTARI
LPDILAQYAPEAGKLSGVRIVRIAVHSRLQRRGLGSKLLRYVEEVASSDGYDWVGAVFGRPDVLGFWLANNYTPIYVSPL
PNKVTGEHNIAVVKPIANKAAKILGGVAAAARKRMLLASHSLYRGLPAEILVEIFSHQVPAQQPLSDIDEWQRYRLSLFL
CGSLEAESVLDVLWLLLVNDILARGRLDLEDPKAELVAVMRILQGKTLADITAAIRVDSSEVRKHLRSLALLLAKRAGIE
AGDSCEAASEPSSISTNPRLATS
>Mature_742_residues
AAVLGRLDLYMVLRQLSAWLTELRGSGYRGLVLLHSTAIGVCASAVAGLLSEVYSQVACIAPAELRRELQERCSRVLSPS
AIEELLGTENDAVLLAVPGLLRPNLLAAAAETVRGGGVVVLVVPRLDVWQPGGGMSTGLYREYLLRSLGRLESLFWADAD
LDTVYSMRLPRGKARKPPGPEGYRARSPVHRKLLEAAATIEQAEALDKIVAHVRSRGRSILVLGDRGRGKSGLLGLAVAY
IVASHMAGFVSVTAPSPANVQSFFRILRLALGKLGTRHWEIRRGNIVIGIAGPWFHVRYHTPDRADPGSFTVVDEAAALG
PSRLRSIARRAPRLLAATTIHGYEGSGRVLAKIAEEILPEPRLVVELRTPVRYPPGDPLEGWLYDTFMLRVSEPEPPSLD
GVELRPAKLDRKELVEKQQLLRAVYSILVSAHYRNEPDDLALLLDAPHHRVYVLLAGNTPVAVAHAALEGPELPVTARIL
PDILAQYAPEAGKLSGVRIVRIAVHSRLQRRGLGSKLLRYVEEVASSDGYDWVGAVFGRPDVLGFWLANNYTPIYVSPLP
NKVTGEHNIAVVKPIANKAAKILGGVAAAARKRMLLASHSLYRGLPAEILVEIFSHQVPAQQPLSDIDEWQRYRLSLFLC
GSLEAESVLDVLWLLLVNDILARGRLDLEDPKAELVAVMRILQGKTLADITAAIRVDSSEVRKHLRSLALLLAKRAGIEA
GDSCEAASEPSSISTNPRLATS

Specific function: Catalyzes the formation of N(4)-acetylcytidine (ac(4)C) at the wobble position of tRNA(Met), by using acetyl-CoA as an acetyl donor and ATP (or GTP) [H]

COG id: COG1444

COG function: function code R; Predicted P-loop ATPase fused to an acetyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 N-acetyltransferase domain [H]

Homologues:

Organism=Homo sapiens, GI221316741, Length=707, Percent_Identity=26.025459688826, Blast_Score=123, Evalue=5e-28,
Organism=Homo sapiens, GI221316723, Length=707, Percent_Identity=26.025459688826, Blast_Score=123, Evalue=7e-28,
Organism=Escherichia coli, GI1788818, Length=603, Percent_Identity=24.0464344941957, Blast_Score=97, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17507621, Length=651, Percent_Identity=24.4239631336406, Blast_Score=126, Evalue=5e-29,
Organism=Saccharomyces cerevisiae, GI6324197, Length=551, Percent_Identity=25.5898366606171, Blast_Score=126, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24640662, Length=660, Percent_Identity=25.6060606060606, Blast_Score=135, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000182
- InterPro:   IPR016181
- InterPro:   IPR007807 [H]

Pfam domain/function: PF00583 Acetyltransf_1; PF05127 DUF699 [H]

EC number: NA

Molecular weight: Translated: 80833; Mature: 80702

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAVLGRLDLYMVLRQLSAWLTELRGSGYRGLVLLHSTAIGVCASAVAGLLSEVYSQVAC
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHH
IAPAELRRELQERCSRVLSPSAIEELLGTENDAVLLAVPGLLRPNLLAAAAETVRGGGVV
CCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCEE
VLVVPRLDVWQPGGGMSTGLYREYLLRSLGRLESLFWADADLDTVYSMRLPRGKARKPPG
EEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCC
PEGYRARSPVHRKLLEAAATIEQAEALDKIVAHVRSRGRSILVLGDRGRGKSGLLGLAVA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHH
YIVASHMAGFVSVTAPSPANVQSFFRILRLALGKLGTRHWEIRRGNIVIGIAGPWFHVRY
HHHHHHHHCEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCEEEEECCCEEEEEE
HTPDRADPGSFTVVDEAAALGPSRLRSIARRAPRLLAATTIHGYEGSGRVLAKIAEEILP
CCCCCCCCCCEEEECHHHHCCHHHHHHHHHHCCHHEEEEEEECCCCCCHHHHHHHHHHCC
EPRLVVELRTPVRYPPGDPLEGWLYDTFMLRVSEPEPPSLDGVELRPAKLDRKELVEKQQ
CCEEEEEECCCCCCCCCCCCCHHEEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHH
LLRAVYSILVSAHYRNEPDDLALLLDAPHHRVYVLLAGNTPVAVAHAALEGPELPVTARI
HHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEEEECCCCEEHHHHHCCCCCCCCHHHH
LPDILAQYAPEAGKLSGVRIVRIAVHSRLQRRGLGSKLLRYVEEVASSDGYDWVGAVFGR
HHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHCCC
PDVLGFWLANNYTPIYVSPLPNKVTGEHNIAVVKPIANKAAKILGGVAAAARKRMLLASH
CCEEEEEEECCCCEEEECCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHH
SLYRGLPAEILVEIFSHQVPAQQPLSDIDEWQRYRLSLFLCGSLEAESVLDVLWLLLVND
HHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHHHHH
ILARGRLDLEDPKAELVAVMRILQGKTLADITAAIRVDSSEVRKHLRSLALLLAKRAGIE
HHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHEEECHHHHHHHHHHHHHHHHHHCCCC
AGDSCEAASEPSSISTNPRLATS
CCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AAVLGRLDLYMVLRQLSAWLTELRGSGYRGLVLLHSTAIGVCASAVAGLLSEVYSQVAC
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHH
IAPAELRRELQERCSRVLSPSAIEELLGTENDAVLLAVPGLLRPNLLAAAAETVRGGGVV
CCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCEE
VLVVPRLDVWQPGGGMSTGLYREYLLRSLGRLESLFWADADLDTVYSMRLPRGKARKPPG
EEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCC
PEGYRARSPVHRKLLEAAATIEQAEALDKIVAHVRSRGRSILVLGDRGRGKSGLLGLAVA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHH
YIVASHMAGFVSVTAPSPANVQSFFRILRLALGKLGTRHWEIRRGNIVIGIAGPWFHVRY
HHHHHHHHCEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCEEEEECCCEEEEEE
HTPDRADPGSFTVVDEAAALGPSRLRSIARRAPRLLAATTIHGYEGSGRVLAKIAEEILP
CCCCCCCCCCEEEECHHHHCCHHHHHHHHHHCCHHEEEEEEECCCCCCHHHHHHHHHHCC
EPRLVVELRTPVRYPPGDPLEGWLYDTFMLRVSEPEPPSLDGVELRPAKLDRKELVEKQQ
CCEEEEEECCCCCCCCCCCCCHHEEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHH
LLRAVYSILVSAHYRNEPDDLALLLDAPHHRVYVLLAGNTPVAVAHAALEGPELPVTARI
HHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEEEECCCCEEHHHHHCCCCCCCCHHHH
LPDILAQYAPEAGKLSGVRIVRIAVHSRLQRRGLGSKLLRYVEEVASSDGYDWVGAVFGR
HHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHCCC
PDVLGFWLANNYTPIYVSPLPNKVTGEHNIAVVKPIANKAAKILGGVAAAARKRMLLASH
CCEEEEEEECCCCEEEECCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHH
SLYRGLPAEILVEIFSHQVPAQQPLSDIDEWQRYRLSLFLCGSLEAESVLDVLWLLLVND
HHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHHHHH
ILARGRLDLEDPKAELVAVMRILQGKTLADITAAIRVDSSEVRKHLRSLALLLAKRAGIE
HHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHEEECHHHHHHHHHHHHHHHHHHCCCC
AGDSCEAASEPSSISTNPRLATS
CCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]