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The map label for this gene is nfo [C]

Identifier: 124027908

GI number: 124027908

Start: 1054193

End: 1055044

Strand: Direct

Name: nfo [C]

Synonym: Hbut_1039

Alternate gene names: 124027908

Gene position: 1054193-1055044 (Clockwise)

Preceding gene: 124027907

Following gene: 124027909

Centisome position: 63.23

GC content: 57.16

Gene sequence:

>852_bases
ATGGCTAGGAGGCTCCACTTTGGTCCCGCTGGGAAACCAACTACGCTCAAGAGTGGCGACTACGTGAAGGCTATAGAGGT
CATAGCGGGGATGGGCCTGGATGCTATAGAGTATGAGGCTGTGCGCGGTGTAAGAATAAGCGAGGCCAAGGCACGGGCGA
TAGCAGAGGCCGCGGAGAGGTACGGGGTCATAGTCTCTATGCACGCCCCCTACTTCATTAACTTTGCCTCGCCCGACAAG
CAGACCGTGGAGAAGAGCCGTGAGAGGCTCATAGCTTCTGTCCGGGCGGCAAGCTGGATGAACGCCTACGCTGTTGTCTT
CCACCCCGGCTACTACAAGGGCAACAGTAGCCCCGAGGAGGCCCTGCAGAGGACCATTGAGAATCTACGCAGCGTGGATG
AGTACATAGAGCAGCAGGGCATCAAGGGTGTCTGGATAGCCCCCGAGACGACTGGGAGGACAGCACAAGTTGGGAGCGTC
GAGGAGGTCGTGGCTATCTGTAGGGAGCTGCGCCACGCACGCCCAGCTGTAGACTGGGCCCACATTCACGCTAGGAGCCT
CGGCAAACACGTGGTATCCGTCGACGATGTTGTCAGGATCGTAGAGTACATTGAGAGGGAGCTAGGCACCTGGGCGGTCA
AGCCGTTACACACGCACTTCAGTAAGATAGGGTACGGGGAGGGCGGCGAAAAGGAGCACCACACCTTGGCGGAGGGGGAG
TACGGTCCTGACTGGAGGATAGTGTGCAGAGCATACAAGAATCTCGGCATAGAGGCAGTAGTGATATCTGAAAGCCCCAT
ACTTGAGCAGGACGCGCTTGTTATGAAGAAGATATGCGAAGATGAAGGGTAA

Upstream 100 bases:

>100_bases
GACCCCTCCTATGGGGGATGGTGTGAACGGACCCGCTGAGCCCCTACAAGCCGTTAAACCCTCCCAAGCGGTAACCCCTT
CCTTATCGGTGAGCCACTCT

Downstream 100 bases:

>100_bases
ACCTAGACACAGCCGGGCTCCGCTACCGTTTTTACCTTCGTCCAAGTACGTGGCTACTATAGGCTCCTTAGGGGTGCCTA
GTAGAGTCTTGGGAGGCTAT

Product: endonuclease IV

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MARRLHFGPAGKPTTLKSGDYVKAIEVIAGMGLDAIEYEAVRGVRISEAKARAIAEAAERYGVIVSMHAPYFINFASPDK
QTVEKSRERLIASVRAASWMNAYAVVFHPGYYKGNSSPEEALQRTIENLRSVDEYIEQQGIKGVWIAPETTGRTAQVGSV
EEVVAICRELRHARPAVDWAHIHARSLGKHVVSVDDVVRIVEYIERELGTWAVKPLHTHFSKIGYGEGGEKEHHTLAEGE
YGPDWRIVCRAYKNLGIEAVVISESPILEQDALVMKKICEDEG

Sequences:

>Translated_283_residues
MARRLHFGPAGKPTTLKSGDYVKAIEVIAGMGLDAIEYEAVRGVRISEAKARAIAEAAERYGVIVSMHAPYFINFASPDK
QTVEKSRERLIASVRAASWMNAYAVVFHPGYYKGNSSPEEALQRTIENLRSVDEYIEQQGIKGVWIAPETTGRTAQVGSV
EEVVAICRELRHARPAVDWAHIHARSLGKHVVSVDDVVRIVEYIERELGTWAVKPLHTHFSKIGYGEGGEKEHHTLAEGE
YGPDWRIVCRAYKNLGIEAVVISESPILEQDALVMKKICEDEG
>Mature_282_residues
ARRLHFGPAGKPTTLKSGDYVKAIEVIAGMGLDAIEYEAVRGVRISEAKARAIAEAAERYGVIVSMHAPYFINFASPDKQ
TVEKSRERLIASVRAASWMNAYAVVFHPGYYKGNSSPEEALQRTIENLRSVDEYIEQQGIKGVWIAPETTGRTAQVGSVE
EVVAICRELRHARPAVDWAHIHARSLGKHVVSVDDVVRIVEYIERELGTWAVKPLHTHFSKIGYGEGGEKEHHTLAEGEY
GPDWRIVCRAYKNLGIEAVVISESPILEQDALVMKKICEDEG

Specific function: Endonuclease IV Plays A Role In DNA Repair. It Cleaves Phosphodiester Bonds At Apurinic Or Apyrimidinic Sites (Ap Sites) To Produce New 5' Ends That Are Base-Free Deoxyribose 5-Phosphate Residues. It Preferentially Attacks Modified Ap Sites Created By Bl

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307 [H]

Pfam domain/function: PF01261 AP_endonuc_2 [H]

EC number: 3.1.21.2

Molecular weight: Translated: 31436; Mature: 31305

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS00729 AP_NUCLEASE_F2_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARRLHFGPAGKPTTLKSGDYVKAIEVIAGMGLDAIEYEAVRGVRISEAKARAIAEAAER
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHH
YGVIVSMHAPYFINFASPDKQTVEKSRERLIASVRAASWMNAYAVVFHPGYYKGNSSPEE
CCEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCHHH
ALQRTIENLRSVDEYIEQQGIKGVWIAPETTGRTAQVGSVEEVVAICRELRHARPAVDWA
HHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHH
HIHARSLGKHVVSVDDVVRIVEYIERELGTWAVKPLHTHFSKIGYGEGGEKEHHTLAEGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECHHHHHHHHHCCCCCCCCCCCCCCCCCC
YGPDWRIVCRAYKNLGIEAVVISESPILEQDALVMKKICEDEG
CCCCHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ARRLHFGPAGKPTTLKSGDYVKAIEVIAGMGLDAIEYEAVRGVRISEAKARAIAEAAER
CCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHH
YGVIVSMHAPYFINFASPDKQTVEKSRERLIASVRAASWMNAYAVVFHPGYYKGNSSPEE
CCEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCHHH
ALQRTIENLRSVDEYIEQQGIKGVWIAPETTGRTAQVGSVEEVVAICRELRHARPAVDWA
HHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHH
HIHARSLGKHVVSVDDVVRIVEYIERELGTWAVKPLHTHFSKIGYGEGGEKEHHTLAEGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECHHHHHHHHHCCCCCCCCCCCCCCCCCC
YGPDWRIVCRAYKNLGIEAVVISESPILEQDALVMKKICEDEG
CCCCHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Ca; Co; Mg; Mn2+; Zn [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Phosphoric ester hydrolysis [C]

Inhibitor: (NH4)2SO4; KCl; NaCl; NH4Cl; p-Hydroxy mercuribenzoate; Potassium phosphate buffer [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]