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The map label for this gene is nfrA2 [H]

Identifier: 124027887

GI number: 124027887

Start: 1028092

End: 1028799

Strand: Reverse

Name: nfrA2 [H]

Synonym: Hbut_1016

Alternate gene names: 124027887

Gene position: 1028799-1028092 (Counterclockwise)

Preceding gene: 124027888

Following gene: 124027886

Centisome position: 61.71

GC content: 57.91

Gene sequence:

>708_bases
ATGTCCTGCTGCATAGAAACAATTTCGCGTCACGTCAGCATCCGCAAATACACAGATGAGCCCGTCAAGCCTGAGGATCT
CGAAGCCATTGTTGAGGCTGCACGGAGGGCACCCACGTCCTGGGGTATACAGCCTTTCACTGTAACCATTGTAACTGATG
GAGAGCTGAAGGCTAAGCTTGCAGAAGCTGTAGGCGGCCAGGAGCACGTAGCTAAGGCCCCGGTGTTCCTCGTGTTTAGC
GTCGACTACCGCAAGCTCGCCGAAGCCGCAAGGATTCACGGCGTGGAGTTTGCCGAGCCCGGGCTGGGCCACCTCCTCAT
AGGGGTGGTGGATGCCGGTATTGCAGCTGGCTGGGCGGCACTCGCAGCGGAGAGTCTGGGCTACGGCATAGTCTTCATAG
CGCTCTACTCGAACCCCTGCCGCATAGCGGAAATCCTCAACCTGCCAGAGCAAGTGCTACCCGTTGTAGGACTATGTGTT
GGGAGGCCTGCTGAGAAGCCATCACCCAAGCCGAGGCAGCCCAGGGAGGTATTCGCGGCAGAGAACCGCTACATAGCCCT
AGACGAGGAGGCCGCCAGGAAGGTCGCATACGTGTTCGGCGAGAAGACACAAAGGATCTACAGCTACGTCCTAAGCAAGG
GCGGTTACTACGAACAGGTTACCAGGAGGCTGCTGGAGTGCGCTAGGAGGAGGGGCTTCAGAATATAA

Upstream 100 bases:

>100_bases
CAAGCACTAGCACCATTTGTTCGCTTACTACGCTTCGATGTTTGGGGCGGGTTATGTATTCTAGTCTGCTAGCTACAGTA
CCCTAGACCGGTGTGCCAGC

Downstream 100 bases:

>100_bases
TAGTAGCCAGAGGTGACCATCGAAAGCATAGGCTCGGAGCTAGGGATAAGAGTAGAGCCCGACACCACAACCCTCACCAT
CTCCCCGGAGCCCCGGTACT

Product: nitroreductase

Products: NA

Alternate protein names: NAD(P)H-dependent FMN reductase; NAD(P)H-dependent nitroreductase; NAD(P)H-dependent oxidoreductase [H]

Number of amino acids: Translated: 235; Mature: 234

Protein sequence:

>235_residues
MSCCIETISRHVSIRKYTDEPVKPEDLEAIVEAARRAPTSWGIQPFTVTIVTDGELKAKLAEAVGGQEHVAKAPVFLVFS
VDYRKLAEAARIHGVEFAEPGLGHLLIGVVDAGIAAGWAALAAESLGYGIVFIALYSNPCRIAEILNLPEQVLPVVGLCV
GRPAEKPSPKPRQPREVFAAENRYIALDEEAARKVAYVFGEKTQRIYSYVLSKGGYYEQVTRRLLECARRRGFRI

Sequences:

>Translated_235_residues
MSCCIETISRHVSIRKYTDEPVKPEDLEAIVEAARRAPTSWGIQPFTVTIVTDGELKAKLAEAVGGQEHVAKAPVFLVFS
VDYRKLAEAARIHGVEFAEPGLGHLLIGVVDAGIAAGWAALAAESLGYGIVFIALYSNPCRIAEILNLPEQVLPVVGLCV
GRPAEKPSPKPRQPREVFAAENRYIALDEEAARKVAYVFGEKTQRIYSYVLSKGGYYEQVTRRLLECARRRGFRI
>Mature_234_residues
SCCIETISRHVSIRKYTDEPVKPEDLEAIVEAARRAPTSWGIQPFTVTIVTDGELKAKLAEAVGGQEHVAKAPVFLVFSV
DYRKLAEAARIHGVEFAEPGLGHLLIGVVDAGIAAGWAALAAESLGYGIVFIALYSNPCRIAEILNLPEQVLPVVGLCVG
RPAEKPSPKPRQPREVFAAENRYIALDEEAARKVAYVFGEKTQRIYSYVLSKGGYYEQVTRRLLECARRRGFRI

Specific function: Reduces FMNH(2) to FMN, with NADPH as reductant. It also reduces nitroaromatic compounds, quinones, chromates and azo dyes. It could supply the reduced form of FMN to luciferase-like protein and contribute to the degradation of aromatic compounds [H]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the flavin oxidoreductase frp family [H]

Homologues:

Organism=Escherichia coli, GI1787075, Length=222, Percent_Identity=36.9369369369369, Blast_Score=130, Evalue=6e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016446
- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: =1.5.1.29 [H]

Molecular weight: Translated: 25854; Mature: 25723

Theoretical pI: Translated: 8.19; Mature: 8.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSCCIETISRHVSIRKYTDEPVKPEDLEAIVEAARRAPTSWGIQPFTVTIVTDGELKAKL
CCHHHHHHHHHCEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCHHHHHH
AEAVGGQEHVAKAPVFLVFSVDYRKLAEAARIHGVEFAEPGLGHLLIGVVDAGIAAGWAA
HHHCCCHHHHHCCCEEEEEECCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LAAESLGYGIVFIALYSNPCRIAEILNLPEQVLPVVGLCVGRPAEKPSPKPRQPREVFAA
HHHHHCCCCEEEEEEECCCHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHC
ENRYIALDEEAARKVAYVFGEKTQRIYSYVLSKGGYYEQVTRRLLECARRRGFRI
CCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SCCIETISRHVSIRKYTDEPVKPEDLEAIVEAARRAPTSWGIQPFTVTIVTDGELKAKL
CHHHHHHHHHCEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCHHHHHH
AEAVGGQEHVAKAPVFLVFSVDYRKLAEAARIHGVEFAEPGLGHLLIGVVDAGIAAGWAA
HHHCCCHHHHHCCCEEEEEECCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LAAESLGYGIVFIALYSNPCRIAEILNLPEQVLPVVGLCVGRPAEKPSPKPRQPREVFAA
HHHHHCCCCEEEEEEECCCHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHC
ENRYIALDEEAARKVAYVFGEKTQRIYSYVLSKGGYYEQVTRRLLECARRRGFRI
CCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377 [H]