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The map label for this gene is radA [H]

Identifier: 124027840

GI number: 124027840

Start: 979368

End: 980339

Strand: Direct

Name: radA [H]

Synonym: Hbut_0967

Alternate gene names: 124027840

Gene position: 979368-980339 (Clockwise)

Preceding gene: 124027835

Following gene: 124027842

Centisome position: 58.74

GC content: 50.31

Gene sequence:

>972_bases
ATGGCTAAACAAGAAATAAAGTCGATAACCGATCTTCCTGGCGTAGGTCCAGCTACAGCTAACAAGCTAATTGAAGCAGG
CTATGCTACCATAGAAGCTATAGCTGTTGCGACGCCACAGGAGCTGAGCGCAGCAGCAGGCATACCGCTAACGGCTGCCC
AGAGAATAATAAAGGCTGCACGTGAAGCTCTCGATATAAGATTCAAGACAGCCCTCGAGGTAAAGAAGGAGCGTATGCAG
ACCAGGAAGATAACAACTGGCAGTAGAAACCTTGACGATCTACTAGGCGGCGGAATAGAGACCAAGACAATAACAGAGTT
CTTTGGCGAGTTTGGTAGTGGAAAGACACAAATATGCCACCAGCTAGCTGTAAACGTGCAGTTACCCATTGAGAAGGGAG
GTCTAAGCACGCAGGACAGGGTAGCTAAGGCTGTCTACATCGATACTGAGGGAACATTCCGGTGGGAACGCTTAGAAAAC
ATGGCGAAGAGATGGGGGCTAGACCCCGACGAGGTTATGTCGAACATATTCTACATAAGAGCCATAAATAGTGACCATCA
GATGGCGATAGTCGACGAACTGTTTAACATAGTGCCCAAGGAGAACATTAAGCTCGTAATAGTGGACTCAGTTACTAGCC
ACTTCAGAGCCGAATACCCGGGCAGGGAGAACCTTGCTGCTAGACAGCAGAAGCTCAACAGACACCTGCACCAGCTTGCA
AAGCTAGCAGAGGTCTATGATCTAGCAGTGGTAATCACGAACCAGGTTATGGCTAGGCCAGACGTCTTCTATGGCGATCC
AACCCAGGCTGTAGGTGGGCATGTATTATACCATGCGCCGGGTGTGAGGGTGCAGCTTAGGAAGAGCAGGGGTAACAAGC
GCATAGCAAGAATAGTGGACGCGCCGCATCTGCCAGAGGGCGAGGCAGTGTTCGTGATAACAGACTACGGCATAGCAGAC
CCGGAGGACTAG

Upstream 100 bases:

>100_bases
GACATGTGCTTTTAAGCTAAGGCCGTCAAGAGAATAGTTTCGGCCGGGTCAAGAGCAGTATATAAAGGTGTTACTACACC
TTCATGCCCGGGATAGTAGG

Downstream 100 bases:

>100_bases
CGAGCTAAGCTAGCACCTCCACACTATGCAACTATTACATGCTGCTATAGCTTCTTCATCTAGCTCTACAACCGATCTTT
TCCAGTGAAACTTGATCATG

Product: DNA repair and recombination protein RadA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MAKQEIKSITDLPGVGPATANKLIEAGYATIEAIAVATPQELSAAAGIPLTAAQRIIKAAREALDIRFKTALEVKKERMQ
TRKITTGSRNLDDLLGGGIETKTITEFFGEFGSGKTQICHQLAVNVQLPIEKGGLSTQDRVAKAVYIDTEGTFRWERLEN
MAKRWGLDPDEVMSNIFYIRAINSDHQMAIVDELFNIVPKENIKLVIVDSVTSHFRAEYPGRENLAARQQKLNRHLHQLA
KLAEVYDLAVVITNQVMARPDVFYGDPTQAVGGHVLYHAPGVRVQLRKSRGNKRIARIVDAPHLPEGEAVFVITDYGIAD
PED

Sequences:

>Translated_323_residues
MAKQEIKSITDLPGVGPATANKLIEAGYATIEAIAVATPQELSAAAGIPLTAAQRIIKAAREALDIRFKTALEVKKERMQ
TRKITTGSRNLDDLLGGGIETKTITEFFGEFGSGKTQICHQLAVNVQLPIEKGGLSTQDRVAKAVYIDTEGTFRWERLEN
MAKRWGLDPDEVMSNIFYIRAINSDHQMAIVDELFNIVPKENIKLVIVDSVTSHFRAEYPGRENLAARQQKLNRHLHQLA
KLAEVYDLAVVITNQVMARPDVFYGDPTQAVGGHVLYHAPGVRVQLRKSRGNKRIARIVDAPHLPEGEAVFVITDYGIAD
PED
>Mature_322_residues
AKQEIKSITDLPGVGPATANKLIEAGYATIEAIAVATPQELSAAAGIPLTAAQRIIKAAREALDIRFKTALEVKKERMQT
RKITTGSRNLDDLLGGGIETKTITEFFGEFGSGKTQICHQLAVNVQLPIEKGGLSTQDRVAKAVYIDTEGTFRWERLENM
AKRWGLDPDEVMSNIFYIRAINSDHQMAIVDELFNIVPKENIKLVIVDSVTSHFRAEYPGRENLAARQQKLNRHLHQLAK
LAEVYDLAVVITNQVMARPDVFYGDPTQAVGGHVLYHAPGVRVQLRKSRGNKRIARIVDAPHLPEGEAVFVITDYGIADP
ED

Specific function: Involved in DNA repair and in homologous recombination. Binds and assemble on single-stranded DNA to form a nucleoprotein filament. Hydrolyzes ATP in a ssDNA-dependent manner and promotes DNA strand exchange between homologous DNA molecules [H]

COG id: COG0468

COG function: function code L; RecA/RadA recombinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the eukaryotic recA-like protein family [H]

Homologues:

Organism=Homo sapiens, GI19924133, Length=313, Percent_Identity=46.3258785942492, Blast_Score=274, Evalue=6e-74,
Organism=Homo sapiens, GI23238219, Length=315, Percent_Identity=42.8571428571429, Blast_Score=247, Evalue=1e-65,
Organism=Homo sapiens, GI256017143, Length=314, Percent_Identity=42.6751592356688, Blast_Score=243, Evalue=2e-64,
Organism=Homo sapiens, GI256017141, Length=314, Percent_Identity=42.6751592356688, Blast_Score=243, Evalue=2e-64,
Organism=Homo sapiens, GI256017145, Length=231, Percent_Identity=46.3203463203463, Blast_Score=199, Evalue=2e-51,
Organism=Homo sapiens, GI17402896, Length=340, Percent_Identity=30.5882352941176, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI19924117, Length=351, Percent_Identity=25.3561253561254, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI46255039, Length=351, Percent_Identity=25.3561253561254, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI10835029, Length=351, Percent_Identity=25.3561253561254, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI153946430, Length=281, Percent_Identity=30.2491103202847, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI4885659, Length=281, Percent_Identity=30.2491103202847, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI153946427, Length=281, Percent_Identity=30.2491103202847, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71997301, Length=306, Percent_Identity=45.7516339869281, Blast_Score=266, Evalue=1e-71,
Organism=Caenorhabditis elegans, GI71997295, Length=306, Percent_Identity=45.7516339869281, Blast_Score=265, Evalue=2e-71,
Organism=Saccharomyces cerevisiae, GI6321027, Length=314, Percent_Identity=41.0828025477707, Blast_Score=253, Evalue=2e-68,
Organism=Saccharomyces cerevisiae, GI6320942, Length=313, Percent_Identity=41.2140575079872, Blast_Score=243, Evalue=3e-65,
Organism=Saccharomyces cerevisiae, GI6320207, Length=188, Percent_Identity=38.2978723404255, Blast_Score=104, Evalue=2e-23,
Organism=Drosophila melanogaster, GI17864108, Length=322, Percent_Identity=43.167701863354, Blast_Score=238, Evalue=3e-63,
Organism=Drosophila melanogaster, GI24651285, Length=275, Percent_Identity=44, Blast_Score=223, Evalue=1e-58,
Organism=Drosophila melanogaster, GI17136506, Length=285, Percent_Identity=30.5263157894737, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24650551, Length=219, Percent_Identity=29.2237442922374, Blast_Score=82, Evalue=4e-16,

Paralogues:

None

Copy number: 1548 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800-1200 (L-broth) 40,000-60,000 (L-broth + Nalidixate) 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013632
- InterPro:   IPR011938
- InterPro:   IPR016467
- InterPro:   IPR020588
- InterPro:   IPR010995
- InterPro:   IPR003583
- InterPro:   IPR020587 [H]

Pfam domain/function: PF08423 Rad51 [H]

EC number: NA

Molecular weight: Translated: 35689; Mature: 35558

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: PS50162 RECA_2 ; PS50163 RECA_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKQEIKSITDLPGVGPATANKLIEAGYATIEAIAVATPQELSAAAGIPLTAAQRIIKAA
CCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHEECCCHHHHHHCCCCHHHHHHHHHHH
REALDIRFKTALEVKKERMQTRKITTGSRNLDDLLGGGIETKTITEFFGEFGSGKTQICH
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCHHHHHHHHHHHCCCHHHHHH
QLAVNVQLPIEKGGLSTQDRVAKAVYIDTEGTFRWERLENMAKRWGLDPDEVMSNIFYIR
HHHHEEEEEECCCCCCCHHHHEEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHEEEEE
AINSDHQMAIVDELFNIVPKENIKLVIVDSVTSHFRAEYPGRENLAARQQKLNRHLHQLA
EECCCCCHHHHHHHHHHCCCCCEEEEEEECHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
KLAEVYDLAVVITNQVMARPDVFYGDPTQAVGGHVLYHAPGVRVQLRKSRGNKRIARIVD
HHHHHHHHHHHHHHHHHCCCCCEECCCCHHHCCEEEEECCCCEEEEECCCCCHHHHHHHC
APHLPEGEAVFVITDYGIADPED
CCCCCCCCEEEEEEECCCCCCCC
>Mature Secondary Structure 
AKQEIKSITDLPGVGPATANKLIEAGYATIEAIAVATPQELSAAAGIPLTAAQRIIKAA
CHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHEECCCHHHHHHCCCCHHHHHHHHHHH
REALDIRFKTALEVKKERMQTRKITTGSRNLDDLLGGGIETKTITEFFGEFGSGKTQICH
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCHHHHHHHHHHHCCCHHHHHH
QLAVNVQLPIEKGGLSTQDRVAKAVYIDTEGTFRWERLENMAKRWGLDPDEVMSNIFYIR
HHHHEEEEEECCCCCCCHHHHEEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHEEEEE
AINSDHQMAIVDELFNIVPKENIKLVIVDSVTSHFRAEYPGRENLAARQQKLNRHLHQLA
EECCCCCHHHHHHHHHHCCCCCEEEEEEECHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
KLAEVYDLAVVITNQVMARPDVFYGDPTQAVGGHVLYHAPGVRVQLRKSRGNKRIARIVD
HHHHHHHHHHHHHHHHHCCCCCEECCCCHHHCCEEEEECCCCEEEEECCCCCHHHHHHHC
APHLPEGEAVFVITDYGIADPED
CCCCCCCCEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA