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The map label for this gene is top6A

Identifier: 124027834

GI number: 124027834

Start: 970927

End: 972090

Strand: Direct

Name: top6A

Synonym: Hbut_0961

Alternate gene names: 124027834

Gene position: 970927-972090 (Clockwise)

Preceding gene: 124027833

Following gene: 124027835

Centisome position: 58.24

GC content: 45.27

Gene sequence:

>1164_bases
GTGGCTGAGGAGTATGTAGACGTAGTCGATATTGAGGCTAGGAAGAAAGCACTAGCGGTGTTCAGGGAGAAGTTTAAGGA
GATACTAGAGCAGGTAATGAGGGGTGAAAACCCCACAATAATGCTTCCAAAGAGGACACTATCAAACACAATATACGATG
AGAAGCGTAAACTACTACTCCTGGGCCCTGAGAAGCTTAAGCGTAGTTTCTTCGACCTTCACGAGTCAAAGAAGTTTATG
CAGACACTTCTAATGGCAAGAATAATATATGAAGCACTTGAACGTAATGAATACCCAACTATCCGTGACCTTTACTATCG
CGGCAAGCACACCATAGTGTATCGTGAGCCTGGCGGGAGAAGACACGAAGAGAACACTTGGGATGAGCAGCGTGAATCAG
ATGCAGTTATACGTGACATAGAGGTATTCACCGGGCTCCTCCGCGAAGAAATGCTAATACTGAGTAAGGAGAAGGGCAAA
GTAGTAGGCAACATGAGGATAAGAAGTGGAGGCGACATTATCGATTTAAGCAAGATGGGTCACGGCGCCTACGCTATAGA
GCCAACACCTGACTTAATAGAGTTCATAGACGTTGATGCCGAGTATGTGCTAGTCGTAGAGAAAGACGCAGTATTTCAGC
AACTTCACAGAATAGGATTCTGGAAGAAGCATCGTGCAATACTAATAACAAGTGCTGGCCAGCCAGACCGTGCAACGAGA
AGATTCGTGCGAAGATTAAACGAAGAGTTAGGCTTACCAGTCTACATACTAACAGACGCGGATCCCTATGGATGGTATAT
CTACAGTGTCTTCAAAATAGGCTCCATAACCCTAAGCTACGAGAGCGAGAGACTAGCTACGCCCAACGCCCGCTTCATAG
GCGTAAGCATGACAGACATCTTTGGCTACAAGAGCAAGAAGCCATACCTAACAGAACAGGAGAGAAGAAACTTCATAATC
AAGGCGAAGGAAGCAGACATAAAGCGTGCCTACGAGCTAAAGAACTACAAGTGGTTCCAGACAAAGAAATGGCAGATAGA
GATAGATATCTTCCTAGAGAAGAAGGCGAAGCTAGAGATAGAGGCTATGACAAGCAAGGGTCTACGATTCCTAGCAGACA
AGTACTTACCAGAGAAGATCGAAACTGGAGACTGGATAGAATAA

Upstream 100 bases:

>100_bases
TATCAGCCATGAAGAGGAGGCTGCGCATAGAGGATGACAAGTTTGCAAAACTAGTAGAGGGTCTAGAGGTAGATATTGAA
GAATAAAGAGGGGGTAGGGC

Downstream 100 bases:

>100_bases
CTACGTAACCACGAGAATCGATAATTCCAGGTAACAGCAATGTAGAAACATGGTGAGGCAGGAAACATGTCCGAGTCCTT
CTTCTGCAGGTTTGGAGTCA

Product: DNA topoisomerase VI subunit A

Products: NA

Alternate protein names: Type II DNA topoisomerase VI subunit A

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MAEEYVDVVDIEARKKALAVFREKFKEILEQVMRGENPTIMLPKRTLSNTIYDEKRKLLLLGPEKLKRSFFDLHESKKFM
QTLLMARIIYEALERNEYPTIRDLYYRGKHTIVYREPGGRRHEENTWDEQRESDAVIRDIEVFTGLLREEMLILSKEKGK
VVGNMRIRSGGDIIDLSKMGHGAYAIEPTPDLIEFIDVDAEYVLVVEKDAVFQQLHRIGFWKKHRAILITSAGQPDRATR
RFVRRLNEELGLPVYILTDADPYGWYIYSVFKIGSITLSYESERLATPNARFIGVSMTDIFGYKSKKPYLTEQERRNFII
KAKEADIKRAYELKNYKWFQTKKWQIEIDIFLEKKAKLEIEAMTSKGLRFLADKYLPEKIETGDWIE

Sequences:

>Translated_387_residues
MAEEYVDVVDIEARKKALAVFREKFKEILEQVMRGENPTIMLPKRTLSNTIYDEKRKLLLLGPEKLKRSFFDLHESKKFM
QTLLMARIIYEALERNEYPTIRDLYYRGKHTIVYREPGGRRHEENTWDEQRESDAVIRDIEVFTGLLREEMLILSKEKGK
VVGNMRIRSGGDIIDLSKMGHGAYAIEPTPDLIEFIDVDAEYVLVVEKDAVFQQLHRIGFWKKHRAILITSAGQPDRATR
RFVRRLNEELGLPVYILTDADPYGWYIYSVFKIGSITLSYESERLATPNARFIGVSMTDIFGYKSKKPYLTEQERRNFII
KAKEADIKRAYELKNYKWFQTKKWQIEIDIFLEKKAKLEIEAMTSKGLRFLADKYLPEKIETGDWIE
>Mature_386_residues
AEEYVDVVDIEARKKALAVFREKFKEILEQVMRGENPTIMLPKRTLSNTIYDEKRKLLLLGPEKLKRSFFDLHESKKFMQ
TLLMARIIYEALERNEYPTIRDLYYRGKHTIVYREPGGRRHEENTWDEQRESDAVIRDIEVFTGLLREEMLILSKEKGKV
VGNMRIRSGGDIIDLSKMGHGAYAIEPTPDLIEFIDVDAEYVLVVEKDAVFQQLHRIGFWKKHRAILITSAGQPDRATRR
FVRRLNEELGLPVYILTDADPYGWYIYSVFKIGSITLSYESERLATPNARFIGVSMTDIFGYKSKKPYLTEQERRNFIIK
AKEADIKRAYELKNYKWFQTKKWQIEIDIFLEKKAKLEIEAMTSKGLRFLADKYLPEKIETGDWIE

Specific function: Relaxes both positive and negative superturns and exhibits a strong decatenase activity

COG id: COG1697

COG function: function code L; DNA topoisomerase VI, subunit A

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TOP6A family

Homologues:

Organism=Homo sapiens, GI6912680, Length=380, Percent_Identity=28.6842105263158, Blast_Score=151, Evalue=8e-37,
Organism=Homo sapiens, GI38201680, Length=314, Percent_Identity=31.2101910828025, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17542064, Length=325, Percent_Identity=28, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI45552753, Length=359, Percent_Identity=28.4122562674095, Blast_Score=119, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TOP6A_HYPBU (A2BLE8)

Other databases:

- EMBL:   CP000493
- RefSeq:   YP_001013154.1
- ProteinModelPortal:   A2BLE8
- SMR:   A2BLE8
- STRING:   A2BLE8
- GeneID:   4782390
- GenomeReviews:   CP000493_GR
- KEGG:   hbu:Hbut_0961
- eggNOG:   arNOG04570
- HOGENOM:   HBG559107
- OMA:   DGDPYGW
- ProtClustDB:   PRK04342
- BioCyc:   HBUT415426:HBUT_0961-MONOMER
- GO:   GO:0005694
- HAMAP:   MF_00132
- InterPro:   IPR002815
- InterPro:   IPR013049
- InterPro:   IPR004085
- InterPro:   IPR022904
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PANTHER:   PTHR10848
- PRINTS:   PR01550
- PRINTS:   PR01552

Pfam domain/function: PF04406 TP6A_N; SSF56726 Spo11/TopoVI_A

EC number: =5.99.1.3

Molecular weight: Translated: 45575; Mature: 45444

Theoretical pI: Translated: 9.23; Mature: 9.23

Prosite motif: NA

Important sites: ACT_SITE 106-106

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEEYVDVVDIEARKKALAVFREKFKEILEQVMRGENPTIMLPKRTLSNTIYDEKRKLLL
CCCCHHHHEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHCCCCEEE
LGPEKLKRSFFDLHESKKFMQTLLMARIIYEALERNEYPTIRDLYYRGKHTIVYREPGGR
ECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCEEEEEECCCCC
RHEENTWDEQRESDAVIRDIEVFTGLLREEMLILSKEKGKVVGNMRIRSGGDIIDLSKMG
CCCCCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCEEEEECCC
HGAYAIEPTPDLIEFIDVDAEYVLVVEKDAVFQQLHRIGFWKKHRAILITSAGQPDRATR
CCEEEECCCHHHHHHHCCCCEEEEEEECHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHH
RFVRRLNEELGLPVYILTDADPYGWYIYSVFKIGSITLSYESERLATPNARFIGVSMTDI
HHHHHHHHHHCCCEEEEECCCCCCEEEEEEEECCEEEEEECCCCCCCCCCEEEEEEHHHH
FGYKSKKPYLTEQERRNFIIKAKEADIKRAYELKNYKWFQTKKWQIEIDIFLEKKAKLEI
HCCCCCCCCCCCHHCCCEEEEECHHHHHHHHHHCCEEEEECEEEEEEEEEEEECCCCEEE
EAMTSKGLRFLADKYLPEKIETGDWIE
EEHHHCCHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
AEEYVDVVDIEARKKALAVFREKFKEILEQVMRGENPTIMLPKRTLSNTIYDEKRKLLL
CCCHHHHEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHCCCCEEE
LGPEKLKRSFFDLHESKKFMQTLLMARIIYEALERNEYPTIRDLYYRGKHTIVYREPGGR
ECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCEEEEEECCCCC
RHEENTWDEQRESDAVIRDIEVFTGLLREEMLILSKEKGKVVGNMRIRSGGDIIDLSKMG
CCCCCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCEEEEECCC
HGAYAIEPTPDLIEFIDVDAEYVLVVEKDAVFQQLHRIGFWKKHRAILITSAGQPDRATR
CCEEEECCCHHHHHHHCCCCEEEEEEECHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHH
RFVRRLNEELGLPVYILTDADPYGWYIYSVFKIGSITLSYESERLATPNARFIGVSMTDI
HHHHHHHHHHCCCEEEEECCCCCCEEEEEEEECCEEEEEECCCCCCCCCCEEEEEEHHHH
FGYKSKKPYLTEQERRNFIIKAKEADIKRAYELKNYKWFQTKKWQIEIDIFLEKKAKLEI
HCCCCCCCCCCCHHCCCEEEEECHHHHHHHHHHCCEEEEECEEEEEEEEEEEECCCCEEE
EAMTSKGLRFLADKYLPEKIETGDWIE
EEHHHCCHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA