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The map label for this gene is folE

Identifier: 124027744

GI number: 124027744

Start: 870429

End: 871010

Strand: Direct

Name: folE

Synonym: Hbut_0868

Alternate gene names: 124027744

Gene position: 870429-871010 (Clockwise)

Preceding gene: 124027738

Following gene: 124027745

Centisome position: 52.21

GC content: 50.34

Gene sequence:

>582_bases
TTGCCCATCGATAAGGCTAAGATAGAGAAGGCTGTGAGAATGATCCTTGAGGCTATCGGGGAGGATCCAGAGCGTGAAGG
GCTCCGTGAGACGCCGCGACGAGTTGCCGACATGTTTGAGGAGCTGCTTGAGGGCTATGACTTCACCGAAGAATACACAT
GGTTTACCGAGGCTACCGACCTGGTTGTGGTTAGCGGTATAAGGTTCTATAGTCTCTGCGAGCATCACCTGCTACCATTC
TTCGGTGTAGCCCATGTAGCTTATCTTCCTCGCGGCAAGGTTATCGGTTTGAGTAAGATTGTGCGTATAGTTAACAAGTA
TTCTAGAAGGCTACAAATCCAGGAGCGTATGACTAAACAGATAGCTGATGAAATCTCCAAGGCTACAGGCTCACCCGACG
TGATGGTTATAACTGAGGCTGTACACCTCTGCATGGCTATGAGGGGTGTACGTAATGGGGCGCCAACTGTCGTAGCTGCA
GTGCGGGGCGAATTTGAGCGCGACCAGAGCCTAAAGGAGGAGGTTTACAGGATTATAGAGCCACATAGACTGTCAAGGGT
TATCGCGCTTAGCTTCTTCTAG

Upstream 100 bases:

>100_bases
GTCTGGGCTCTATGCGCTAGTATAAATATATTAGTTCTTGGCTTTGTTGTGCTCCAAGGGGCTGTAAGGAGACATTACAG
GGCCTAGTGGGGTGGCAGGC

Downstream 100 bases:

>100_bases
CCCGCTTTTCTCTTCCATTTCCACGTTTTAGCTCATACAATGCCATTACAAGACTTGCAACCACGTTATAGCTCATTCCT
TCAACTCCAACTGGTATGAC

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I

Number of amino acids: Translated: 193; Mature: 192

Protein sequence:

>193_residues
MPIDKAKIEKAVRMILEAIGEDPEREGLRETPRRVADMFEELLEGYDFTEEYTWFTEATDLVVVSGIRFYSLCEHHLLPF
FGVAHVAYLPRGKVIGLSKIVRIVNKYSRRLQIQERMTKQIADEISKATGSPDVMVITEAVHLCMAMRGVRNGAPTVVAA
VRGEFERDQSLKEEVYRIIEPHRLSRVIALSFF

Sequences:

>Translated_193_residues
MPIDKAKIEKAVRMILEAIGEDPEREGLRETPRRVADMFEELLEGYDFTEEYTWFTEATDLVVVSGIRFYSLCEHHLLPF
FGVAHVAYLPRGKVIGLSKIVRIVNKYSRRLQIQERMTKQIADEISKATGSPDVMVITEAVHLCMAMRGVRNGAPTVVAA
VRGEFERDQSLKEEVYRIIEPHRLSRVIALSFF
>Mature_192_residues
PIDKAKIEKAVRMILEAIGEDPEREGLRETPRRVADMFEELLEGYDFTEEYTWFTEATDLVVVSGIRFYSLCEHHLLPFF
GVAHVAYLPRGKVIGLSKIVRIVNKYSRRLQIQERMTKQIADEISKATGSPDVMVITEAVHLCMAMRGVRNGAPTVVAAV
RGEFERDQSLKEEVYRIIEPHRLSRVIALSFF

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family

Homologues:

Organism=Homo sapiens, GI66932968, Length=183, Percent_Identity=40.9836065573771, Blast_Score=146, Evalue=1e-35,
Organism=Homo sapiens, GI4503949, Length=183, Percent_Identity=40.9836065573771, Blast_Score=146, Evalue=1e-35,
Organism=Homo sapiens, GI66932970, Length=153, Percent_Identity=39.8692810457516, Blast_Score=120, Evalue=8e-28,
Organism=Homo sapiens, GI66932972, Length=144, Percent_Identity=41.6666666666667, Blast_Score=119, Evalue=2e-27,
Organism=Escherichia coli, GI1788476, Length=190, Percent_Identity=34.7368421052632, Blast_Score=106, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17560486, Length=177, Percent_Identity=41.8079096045198, Blast_Score=147, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6321706, Length=174, Percent_Identity=41.9540229885057, Blast_Score=142, Evalue=3e-35,
Organism=Drosophila melanogaster, GI24656782, Length=179, Percent_Identity=41.8994413407821, Blast_Score=138, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24656791, Length=179, Percent_Identity=41.8994413407821, Blast_Score=138, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24656796, Length=179, Percent_Identity=41.8994413407821, Blast_Score=138, Evalue=3e-33,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): GCH1_HYPBU (A2BL58)

Other databases:

- EMBL:   CP000493
- RefSeq:   YP_001013064.1
- ProteinModelPortal:   A2BL58
- SMR:   A2BL58
- STRING:   A2BL58
- GeneID:   4782451
- GenomeReviews:   CP000493_GR
- KEGG:   hbu:Hbut_0868
- eggNOG:   arNOG12430
- HOGENOM:   HBG370191
- OMA:   QERICAQ
- PhylomeDB:   A2BL58
- ProtClustDB:   CLSK964527
- BioCyc:   HBUT415426:HBUT_0868-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00223
- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234
- TIGRFAMs:   TIGR00063

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =3.5.4.16

Molecular weight: Translated: 22099; Mature: 21968

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIDKAKIEKAVRMILEAIGEDPEREGLRETPRRVADMFEELLEGYDFTEEYTWFTEATD
CCCCHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
LVVVSGIRFYSLCEHHLLPFFGVAHVAYLPRGKVIGLSKIVRIVNKYSRRLQIQERMTKQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHH
IADEISKATGSPDVMVITEAVHLCMAMRGVRNGAPTVVAAVRGEFERDQSLKEEVYRIIE
HHHHHHHHCCCCCEEHHHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHHC
PHRLSRVIALSFF
HHHHHHHHHHHCC
>Mature Secondary Structure 
PIDKAKIEKAVRMILEAIGEDPEREGLRETPRRVADMFEELLEGYDFTEEYTWFTEATD
CCCHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
LVVVSGIRFYSLCEHHLLPFFGVAHVAYLPRGKVIGLSKIVRIVNKYSRRLQIQERMTKQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHH
IADEISKATGSPDVMVITEAVHLCMAMRGVRNGAPTVVAAVRGEFERDQSLKEEVYRIIE
HHHHHHHHCCCCCEEHHHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHHC
PHRLSRVIALSFF
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA