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The map label for this gene is 124027512

Identifier: 124027512

GI number: 124027512

Start: 606840

End: 607760

Strand: Reverse

Name: 124027512

Synonym: Hbut_0630

Alternate gene names: NA

Gene position: 607760-606840 (Counterclockwise)

Preceding gene: 124027520

Following gene: 124027511

Centisome position: 36.45

GC content: 59.39

Gene sequence:

>921_bases
GTGCACGTGGGGTATGGGAGACTTAGCGCCGCCGAGAGCAGGCCCATCGAGATCAAGCCGTTGTTCCACTACTGGCCGAA
CAGTACGGCGCTAACGTTCTCTGGGTGGGGCTGCAACTTCTACTGCCCATGGTGCCAGAACTATCCCCTCAGCTTCCGCC
ACCCACGCCCCGACGACCCGGTCGTGGAGCCCGAGGTGCTCGTGAGGGAGGCCCTGCGAGCCGGCGATGAGGGTCTATGC
GCGAGCTTCAATGAGCCAGCTACGCTCTTCGACTATCTCGTCGAGGTGTTCAGCCTGGGCCGCCGCTACGGCCTCTACGC
GACAATTGTCACCAACGCATACTTCACCCTGAAGGCTGTAGACGTGCTGGTCTCCGAGGCGGGGGTTGACGGGTGGAGCA
TAGACATCAAGGGCTGCCCGGGGATGAAGGGGAAGGGGATACTCCCCGCGATAGACCATAGCGTGGTGTTCCGGAATGCG
AGGAGGATAATAGACCTCGGCGGCCACGTAGAAATGGTCCACCTAGTGGTGACAGGGGCAAACGACGACCCAGAATGCTT
TGAGTGGATAATAGACCAGCACATATCAAACCTTGGCCACCAGGTACCACTCCACATCAACCGGTACTACCCAGCCCACC
GCTGGCACAAGCCGCCAACACCCATAGGGAAGCTGCTAGAGTGGAGGGAGAAGGCTAGGAGGGCCGGCATAGAGTTCGTA
TACGTTGGCAACGTGGGCGACCCGGAGCTAGAGTCTACCCGGTGCCCAAGGTGCGGCAAGCTGCTCATAAGAAGGGAGGG
GTACCGCGTCACGTACTACAACCTAGACTGCAGCAATGGTAGGTGCCGCTGTCCACGTTGTGGATACCCTATACCACTAC
GTGGCACATACATAGCAGGAAAATCAATACCGAGCCGCTGA

Upstream 100 bases:

>100_bases
GCCGGGGTTTGCCAGGTGCCTGGTTTGCGAGCGCCGGTGCCCCATAGCGCCTGGTGCCCGTGGCGCTTGCGGCAACTACC
TCAACGAGGGAGGCGTACTA

Downstream 100 bases:

>100_bases
TGAAACATGCTAAAAACTAGGATTGGCATTACTCCCCCGAGGGGTTTACACGTAAACCGCCCAAGTGTAACGTAGGCAAT
ACGACGCATCCTACCCTAGG

Product: pyruvate-formate lyase-activating enzyme, PflA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MHVGYGRLSAAESRPIEIKPLFHYWPNSTALTFSGWGCNFYCPWCQNYPLSFRHPRPDDPVVEPEVLVREALRAGDEGLC
ASFNEPATLFDYLVEVFSLGRRYGLYATIVTNAYFTLKAVDVLVSEAGVDGWSIDIKGCPGMKGKGILPAIDHSVVFRNA
RRIIDLGGHVEMVHLVVTGANDDPECFEWIIDQHISNLGHQVPLHINRYYPAHRWHKPPTPIGKLLEWREKARRAGIEFV
YVGNVGDPELESTRCPRCGKLLIRREGYRVTYYNLDCSNGRCRCPRCGYPIPLRGTYIAGKSIPSR

Sequences:

>Translated_306_residues
MHVGYGRLSAAESRPIEIKPLFHYWPNSTALTFSGWGCNFYCPWCQNYPLSFRHPRPDDPVVEPEVLVREALRAGDEGLC
ASFNEPATLFDYLVEVFSLGRRYGLYATIVTNAYFTLKAVDVLVSEAGVDGWSIDIKGCPGMKGKGILPAIDHSVVFRNA
RRIIDLGGHVEMVHLVVTGANDDPECFEWIIDQHISNLGHQVPLHINRYYPAHRWHKPPTPIGKLLEWREKARRAGIEFV
YVGNVGDPELESTRCPRCGKLLIRREGYRVTYYNLDCSNGRCRCPRCGYPIPLRGTYIAGKSIPSR
>Mature_306_residues
MHVGYGRLSAAESRPIEIKPLFHYWPNSTALTFSGWGCNFYCPWCQNYPLSFRHPRPDDPVVEPEVLVREALRAGDEGLC
ASFNEPATLFDYLVEVFSLGRRYGLYATIVTNAYFTLKAVDVLVSEAGVDGWSIDIKGCPGMKGKGILPAIDHSVVFRNA
RRIIDLGGHVEMVHLVVTGANDDPECFEWIIDQHISNLGHQVPLHINRYYPAHRWHKPPTPIGKLLEWREKARRAGIEFV
YVGNVGDPELESTRCPRCGKLLIRREGYRVTYYNLDCSNGRCRCPRCGYPIPLRGTYIAGKSIPSR

Specific function: Unknown

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR016431
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 34608; Mature: 34608

Theoretical pI: Translated: 8.16; Mature: 8.16

Prosite motif: PS00086 CYTOCHROME_P450

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.9 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
3.9 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHVGYGRLSAAESRPIEIKPLFHYWPNSTALTFSGWGCNFYCPWCQNYPLSFRHPRPDDP
CCCCCCCCCCCCCCCEEEEEEEEECCCCCEEEEECCCCCEECCCCCCCCCCCCCCCCCCC
VVEPEVLVREALRAGDEGLCASFNEPATLFDYLVEVFSLGRRYGLYATIVTNAYFTLKAV
CCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCHHCCEEEEEECCCEEHHHHH
DVLVSEAGVDGWSIDIKGCPGMKGKGILPAIDHSVVFRNARRIIDLGGHVEMVHLVVTGA
HHHHHHCCCCCEEEEECCCCCCCCCCCCCHHCHHHHHHCCHHEEECCCCEEEEEEEEECC
NDDPECFEWIIDQHISNLGHQVPLHINRYYPAHRWHKPPTPIGKLLEWREKARRAGIEFV
CCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEE
YVGNVGDPELESTRCPRCGKLLIRREGYRVTYYNLDCSNGRCRCPRCGYPIPLRGTYIAG
EECCCCCCCCCCCCCCHHHHEEEEECCCEEEEEEEECCCCCEECCCCCCCCCCCEEEEEC
KSIPSR
CCCCCC
>Mature Secondary Structure
MHVGYGRLSAAESRPIEIKPLFHYWPNSTALTFSGWGCNFYCPWCQNYPLSFRHPRPDDP
CCCCCCCCCCCCCCCEEEEEEEEECCCCCEEEEECCCCCEECCCCCCCCCCCCCCCCCCC
VVEPEVLVREALRAGDEGLCASFNEPATLFDYLVEVFSLGRRYGLYATIVTNAYFTLKAV
CCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCHHCCEEEEEECCCEEHHHHH
DVLVSEAGVDGWSIDIKGCPGMKGKGILPAIDHSVVFRNARRIIDLGGHVEMVHLVVTGA
HHHHHHCCCCCEEEEECCCCCCCCCCCCCHHCHHHHHHCCHHEEECCCCEEEEEEEEECC
NDDPECFEWIIDQHISNLGHQVPLHINRYYPAHRWHKPPTPIGKLLEWREKARRAGIEFV
CCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEE
YVGNVGDPELESTRCPRCGKLLIRREGYRVTYYNLDCSNGRCRCPRCGYPIPLRGTYIAG
EECCCCCCCCCCCCCCHHHHEEEEECCCEEEEEEEECCCCCEECCCCCCCCCCCEEEEEC
KSIPSR
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]