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The map label for this gene is truD [H]

Identifier: 124027510

GI number: 124027510

Start: 604553

End: 605647

Strand: Reverse

Name: truD [H]

Synonym: Hbut_0628

Alternate gene names: 124027510

Gene position: 605647-604553 (Counterclockwise)

Preceding gene: 124027511

Following gene: 124027508

Centisome position: 36.33

GC content: 60.73

Gene sequence:

>1095_bases
GTGCTTGACTCCGAGCCTGTGGCATGCATAGCGGCGCCCCGGGAGAGGAGCTTCATAGTGGTTGAGGATCACGGCCTGGA
GCTGGGAGGCACTGGGCGCTTCTGCGTCTACATCGTTGGCAAGAGGAGCCTATCTACCCATGAGGCTCTGCGCCTCCTAG
CCCGCGCCCTCGGCGTCAAAGCATCCCTCCTGAAGGCTCTAGGGCTCAAGGACACTGAAGCTACCACTGTGCAAGCTGTA
TGTACGCCGTGCCCCCAGGAGCCGCCACGCTACATCGTCCTCGACAATCTGTGGGCTCGTCTCGCCGGCCGCCTCGAGGC
GTGTCCGCGGCCGGGAAGGCTCCGGGGTAACAGGTTCACAATAGTGCTCGAAACCGGTGATACCGGGGGGCTTCTCGGGG
CCCTGGATGTGCTTCGGGGTAGGAGGCTCCCAGCATACTATGGTTATCAGAGGTTTGGGACGAGACGCCCGAACACGCAT
ACAGTCGGCATACTATTGTTGAGGGGCGAGGTTGACGCGGCACTCTACGAGGCTGTAGCCGCCGCGTACCCGGACGAGTC
TAGCGTCATCACACGGTGCAGACTCTCCATGTGGAGGGATGGGGGTTGCCCACGGGGAATGTATGAGGCGTGGATGGCCG
AGTCGGCTGGGGACCCCCTGGAGGCTATTCGCCGCATACCACGCAGCATAATGGAGGTTTTCATCGGGGCTGTACAAGCA
TACATCTTCAACCTCTACCTCTCGCTCCGAACACGCTACGGCTATGACCTAGGCGAGAAGCTCCGCGGCGAACGGCAAGA
GGACAGTGGGAGGCCCCTAGCACTAGTACCAGGCATAGGCTACAGAATCGGCGTAGACGGCGAGGCCAGGAAGCTGCTAA
TGGAGGCCATCGAACTTGCCGGCCTAACGCCCAGGGGAGCTCGCAGAGCCGCCAAGCTATGTGCCACGGCTAAAGCCATA
CTGGAGGCCAGTCTACACGGTAGCACGCGAGCTGAAAGCGCAGGTCCTCAGCAGCAACCGCACAGCCGTATCATTCTGGC
TCGAGAGGGGCATGTATGCAACTATACTGTTAAGGGAGCTTGTAAAAGTCCCTGA

Upstream 100 bases:

>100_bases
CTATCCCGGGTCTAATCGAAACATCCGGGTACCAGTGCGGTGGTGTCCCGTGTATCCTGCCTCGAGTATTCTTGATCTAC
ACGTCGGGATATGGTACCGT

Downstream 100 bases:

>100_bases
ATGCATATGAGGTGGGAATATATGTAATGTTAACCGGTACTTTCACATACCTTGTCCCCGATACAACATCCACCTCCTTA
ACTAAAGGTTTTATTACCAG

Product: putative tRNA pseudouridine synthase D, TruD

Products: NA

Alternate protein names: tRNA pseudouridylate synthase D; tRNA-uridine isomerase D [H]

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MLDSEPVACIAAPRERSFIVVEDHGLELGGTGRFCVYIVGKRSLSTHEALRLLARALGVKASLLKALGLKDTEATTVQAV
CTPCPQEPPRYIVLDNLWARLAGRLEACPRPGRLRGNRFTIVLETGDTGGLLGALDVLRGRRLPAYYGYQRFGTRRPNTH
TVGILLLRGEVDAALYEAVAAAYPDESSVITRCRLSMWRDGGCPRGMYEAWMAESAGDPLEAIRRIPRSIMEVFIGAVQA
YIFNLYLSLRTRYGYDLGEKLRGERQEDSGRPLALVPGIGYRIGVDGEARKLLMEAIELAGLTPRGARRAAKLCATAKAI
LEASLHGSTRAESAGPQQQPHSRIILAREGHVCNYTVKGACKSP

Sequences:

>Translated_364_residues
MLDSEPVACIAAPRERSFIVVEDHGLELGGTGRFCVYIVGKRSLSTHEALRLLARALGVKASLLKALGLKDTEATTVQAV
CTPCPQEPPRYIVLDNLWARLAGRLEACPRPGRLRGNRFTIVLETGDTGGLLGALDVLRGRRLPAYYGYQRFGTRRPNTH
TVGILLLRGEVDAALYEAVAAAYPDESSVITRCRLSMWRDGGCPRGMYEAWMAESAGDPLEAIRRIPRSIMEVFIGAVQA
YIFNLYLSLRTRYGYDLGEKLRGERQEDSGRPLALVPGIGYRIGVDGEARKLLMEAIELAGLTPRGARRAAKLCATAKAI
LEASLHGSTRAESAGPQQQPHSRIILAREGHVCNYTVKGACKSP
>Mature_364_residues
MLDSEPVACIAAPRERSFIVVEDHGLELGGTGRFCVYIVGKRSLSTHEALRLLARALGVKASLLKALGLKDTEATTVQAV
CTPCPQEPPRYIVLDNLWARLAGRLEACPRPGRLRGNRFTIVLETGDTGGLLGALDVLRGRRLPAYYGYQRFGTRRPNTH
TVGILLLRGEVDAALYEAVAAAYPDESSVITRCRLSMWRDGGCPRGMYEAWMAESAGDPLEAIRRIPRSIMEVFIGAVQA
YIFNLYLSLRTRYGYDLGEKLRGERQEDSGRPLALVPGIGYRIGVDGEARKLLMEAIELAGLTPRGARRAAKLCATAKAI
LEASLHGSTRAESAGPQQQPHSRIILAREGHVCNYTVKGACKSP

Specific function: Could be responsible for synthesis of pseudouridine from uracil-13 in transfer RNAs [H]

COG id: COG0585

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRUD domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001656
- InterPro:   IPR020119
- InterPro:   IPR011760 [H]

Pfam domain/function: PF01142 TruD [H]

EC number: NA

Molecular weight: Translated: 39728; Mature: 39728

Theoretical pI: Translated: 9.34; Mature: 9.34

Prosite motif: PS50984 TRUD ; PS01268 UPF0024

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDSEPVACIAAPRERSFIVVEDHGLELGGTGRFCVYIVGKRSLSTHEALRLLARALGVK
CCCCCCEEEEECCCCCCEEEEECCCEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHCHH
ASLLKALGLKDTEATTVQAVCTPCPQEPPRYIVLDNLWARLAGRLEACPRPGRLRGNRFT
HHHHHHHCCCCCCCCEEEEEECCCCCCCCCEEEEHHHHHHHHHHHHCCCCCCCCCCCEEE
IVLETGDTGGLLGALDVLRGRRLPAYYGYQRFGTRRPNTHTVGILLLRGEVDAALYEAVA
EEEEECCCCCHHHHHHHHCCCCCCHHHCHHHHCCCCCCCEEEEEEEEECCHHHHHHHHHH
AAYPDESSVITRCRLSMWRDGGCPRGMYEAWMAESAGDPLEAIRRIPRSIMEVFIGAVQA
HCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
YIFNLYLSLRTRYGYDLGEKLRGERQEDSGRPLALVPGIGYRIGVDGEARKLLMEAIELA
HHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCEEEECCCCEEECCCHHHHHHHHHHHHHC
GLTPRGARRAAKLCATAKAILEASLHGSTRAESAGPQQQPHSRIILAREGHVCNYTVKGA
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCEEEEEECCC
CKSP
CCCC
>Mature Secondary Structure
MLDSEPVACIAAPRERSFIVVEDHGLELGGTGRFCVYIVGKRSLSTHEALRLLARALGVK
CCCCCCEEEEECCCCCCEEEEECCCEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHCHH
ASLLKALGLKDTEATTVQAVCTPCPQEPPRYIVLDNLWARLAGRLEACPRPGRLRGNRFT
HHHHHHHCCCCCCCCEEEEEECCCCCCCCCEEEEHHHHHHHHHHHHCCCCCCCCCCCEEE
IVLETGDTGGLLGALDVLRGRRLPAYYGYQRFGTRRPNTHTVGILLLRGEVDAALYEAVA
EEEEECCCCCHHHHHHHHCCCCCCHHHCHHHHCCCCCCCEEEEEEEEECCHHHHHHHHHH
AAYPDESSVITRCRLSMWRDGGCPRGMYEAWMAESAGDPLEAIRRIPRSIMEVFIGAVQA
HCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
YIFNLYLSLRTRYGYDLGEKLRGERQEDSGRPLALVPGIGYRIGVDGEARKLLMEAIELA
HHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCEEEECCCCEEECCCHHHHHHHHHHHHHC
GLTPRGARRAAKLCATAKAILEASLHGSTRAESAGPQQQPHSRIILAREGHVCNYTVKGA
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCEEEEEECCC
CKSP
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA