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The map label for this gene is livK [C]

Identifier: 124027499

GI number: 124027499

Start: 592039

End: 593364

Strand: Direct

Name: livK [C]

Synonym: Hbut_0616

Alternate gene names: 124027499

Gene position: 592039-593364 (Clockwise)

Preceding gene: 124027495

Following gene: 124027500

Centisome position: 35.51

GC content: 51.36

Gene sequence:

>1326_bases
ATGAAAGGATTTAGAGCAATCTCAATATATGTTGTTGTGGGCCTGCTAATACTGGGCCTGCTAGTAGGCCTAGCAGTAGG
CTACTTTATTGGTGGCGGCGGCGGTGAGAGTAAGTCAGCTACTAGTACACCAGCCTTCAACGAAAGCTCAAGCGTAAACT
TTACTGCTGCAGGAGGCGGCCTTCAGGGCGAGATACCTATCGGCATATTGCTCCCGCAGACGGGAAGCTTGGCGCAGGAT
GGAAGGGACAATATTAAGGCTGCCAAGCTGGCAATAGAAGACTTTAACAAGCTACTAGAGTCGATAGGTGCTCCATTCCG
CTTTAAGGCTATAGTTGCTGATAGTGGTACTGACCCCAATAAGGCGCTTGCAGCACTACAGACACTCTACAACCAGCACC
ACGTACAGATTGTCGTGGGTACTGATAGCAGCTGGGTGCTAAGCGGCGTAATGAGCTTTGCCAACGAGCACCACATAGTC
ATGATAAGCCCAGCCAGTACGAGCCCTGCGCTAGCACTGAAGGACTACATATTCAGGATCGTTGGCAACGATAAGGGTCA
GGGTAAGGCGCTAGCAGCACTAGTTCACAATAATGGGTTCAACGCTGCTGTCGTGATATTCAGAGACGAAGCTTATGGCC
GTGGCATAGCTGAGGCATTCAAGGAGAACTTTGAAGCCTTGGGTGGTACAGCGGTCCTAGTACCGTATAACCCACAGAAG
AGCGACTATGCTCCAGAGGTTCAGAAGCTAGCATCGGAGGTTGAGAAGCTCCTCAACGAGGGTAAAAATGTTGCAGTTAT
CATCGTCGCGTTCGAAACAGACGGGATAAACATACTGAGCCATGCAGCCGATATACCGGTGCTAAGCCAGGTAAAGTGGT
TTGCCAGCGAGTCTATCAGGTCGCCAGCGCTGCTCAAGGCCCCTGACAAGGTTAGACAGTTTCTAGCCAAGGTGGAGCTT
GAGGGTACATTCCCAATACCGCCCAAGAGCCCGCTAGGTGAATGGTTTGAAAAGCACTTCGAGGAGGTATACGGCCATCC
GCCAGAGTCTCCGTTCTCGCCATACGCTTATGATGCCGCCTACCTGGCATGCCTGGCTGTAACCATTGCAGGTAAGTACG
ACGGTACGGTCATAGCTAAGGTGTTGCCGGAGGTGGCTAAGAACTACTATGGTGTTACGGGCTGGAAGTTGCTAGACGAG
AATGGTGACCTCAAGTACCAGGAGTACAGCATCTGGAAGTACGTCTGCGAAGGGGGATCCTGCGAGTTCATCGATATCGC
CATATACGATCCACAAAGTGGCAAGATACAGCAGATAGCAGGCTAG

Upstream 100 bases:

>100_bases
GAGAGACATGCGAATGATTTTATCATTATTTTATATCATTTACATGTAGAAATACTTTTAATATGGGGTGATTGGAATCC
CCGGACGAGAGAGTGGGATC

Downstream 100 bases:

>100_bases
ACCTAATCTAAACGCCTAGTACACATCATAGCCCAAAGCCTCTTTTTTACAAGCTTCATGGACACGGCATGGGCTTATTA
GGATTGTTAGGGGATGGCAG

Product: ABC-type branched-chain amino acid transport, periplasmic component

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 441; Mature: 441

Protein sequence:

>441_residues
MKGFRAISIYVVVGLLILGLLVGLAVGYFIGGGGGESKSATSTPAFNESSSVNFTAAGGGLQGEIPIGILLPQTGSLAQD
GRDNIKAAKLAIEDFNKLLESIGAPFRFKAIVADSGTDPNKALAALQTLYNQHHVQIVVGTDSSWVLSGVMSFANEHHIV
MISPASTSPALALKDYIFRIVGNDKGQGKALAALVHNNGFNAAVVIFRDEAYGRGIAEAFKENFEALGGTAVLVPYNPQK
SDYAPEVQKLASEVEKLLNEGKNVAVIIVAFETDGINILSHAADIPVLSQVKWFASESIRSPALLKAPDKVRQFLAKVEL
EGTFPIPPKSPLGEWFEKHFEEVYGHPPESPFSPYAYDAAYLACLAVTIAGKYDGTVIAKVLPEVAKNYYGVTGWKLLDE
NGDLKYQEYSIWKYVCEGGSCEFIDIAIYDPQSGKIQQIAG

Sequences:

>Translated_441_residues
MKGFRAISIYVVVGLLILGLLVGLAVGYFIGGGGGESKSATSTPAFNESSSVNFTAAGGGLQGEIPIGILLPQTGSLAQD
GRDNIKAAKLAIEDFNKLLESIGAPFRFKAIVADSGTDPNKALAALQTLYNQHHVQIVVGTDSSWVLSGVMSFANEHHIV
MISPASTSPALALKDYIFRIVGNDKGQGKALAALVHNNGFNAAVVIFRDEAYGRGIAEAFKENFEALGGTAVLVPYNPQK
SDYAPEVQKLASEVEKLLNEGKNVAVIIVAFETDGINILSHAADIPVLSQVKWFASESIRSPALLKAPDKVRQFLAKVEL
EGTFPIPPKSPLGEWFEKHFEEVYGHPPESPFSPYAYDAAYLACLAVTIAGKYDGTVIAKVLPEVAKNYYGVTGWKLLDE
NGDLKYQEYSIWKYVCEGGSCEFIDIAIYDPQSGKIQQIAG
>Mature_441_residues
MKGFRAISIYVVVGLLILGLLVGLAVGYFIGGGGGESKSATSTPAFNESSSVNFTAAGGGLQGEIPIGILLPQTGSLAQD
GRDNIKAAKLAIEDFNKLLESIGAPFRFKAIVADSGTDPNKALAALQTLYNQHHVQIVVGTDSSWVLSGVMSFANEHHIV
MISPASTSPALALKDYIFRIVGNDKGQGKALAALVHNNGFNAAVVIFRDEAYGRGIAEAFKENFEALGGTAVLVPYNPQK
SDYAPEVQKLASEVEKLLNEGKNVAVIIVAFETDGINILSHAADIPVLSQVKWFASESIRSPALLKAPDKVRQFLAKVEL
EGTFPIPPKSPLGEWFEKHFEEVYGHPPESPFSPYAYDAAYLACLAVTIAGKYDGTVIAKVLPEVAKNYYGVTGWKLLDE
NGDLKYQEYSIWKYVCEGGSCEFIDIAIYDPQSGKIQQIAG

Specific function: This Protein Is A Component Of The Leucine-Specific Transport System, Which Is One Of The Two Periplasmic Binding Protein-Dependent Transport Systems Of The High-Affinity Transport Of The Branched-Chain Amino Acids In E.Coli. [C]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 47279; Mature: 47279

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGFRAISIYVVVGLLILGLLVGLAVGYFIGGGGGESKSATSTPAFNESSSVNFTAAGGG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCEEEEECCCC
LQGEIPIGILLPQTGSLAQDGRDNIKAAKLAIEDFNKLLESIGAPFRFKAIVADSGTDPN
CCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHH
KALAALQTLYNQHHVQIVVGTDSSWVLSGVMSFANEHHIVMISPASTSPALALKDYIFRI
HHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHEE
VGNDKGQGKALAALVHNNGFNAAVVIFRDEAYGRGIAEAFKENFEALGGTAVLVPYNPQK
ECCCCCCCCEEEEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEEECCCCC
SDYAPEVQKLASEVEKLLNEGKNVAVIIVAFETDGINILSHAADIPVLSQVKWFASESIR
CCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHCCCCHHHHHHHHHHHHCC
SPALLKAPDKVRQFLAKVELEGTFPIPPKSPLGEWFEKHFEEVYGHPPESPFSPYAYDAA
CCCEECCCHHHHHHHHHEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
YLACLAVTIAGKYDGTVIAKVLPEVAKNYYGVTGWKLLDENGDLKYQEYSIWKYVCEGGS
HHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCEEEEHHHHHHHEECCCC
CEFIDIAIYDPQSGKIQQIAG
EEEEEEEEECCCCCCCCCCCC
>Mature Secondary Structure
MKGFRAISIYVVVGLLILGLLVGLAVGYFIGGGGGESKSATSTPAFNESSSVNFTAAGGG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCEEEEECCCC
LQGEIPIGILLPQTGSLAQDGRDNIKAAKLAIEDFNKLLESIGAPFRFKAIVADSGTDPN
CCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHH
KALAALQTLYNQHHVQIVVGTDSSWVLSGVMSFANEHHIVMISPASTSPALALKDYIFRI
HHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHEE
VGNDKGQGKALAALVHNNGFNAAVVIFRDEAYGRGIAEAFKENFEALGGTAVLVPYNPQK
ECCCCCCCCEEEEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEEECCCCC
SDYAPEVQKLASEVEKLLNEGKNVAVIIVAFETDGINILSHAADIPVLSQVKWFASESIR
CCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHCCCCHHHHHHHHHHHHCC
SPALLKAPDKVRQFLAKVELEGTFPIPPKSPLGEWFEKHFEEVYGHPPESPFSPYAYDAA
CCCEECCCHHHHHHHHHEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
YLACLAVTIAGKYDGTVIAKVLPEVAKNYYGVTGWKLLDENGDLKYQEYSIWKYVCEGGS
HHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCEEEEHHHHHHHEECCCC
CEFIDIAIYDPQSGKIQQIAG
EEEEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]