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The map label for this gene is tgtA [H]

Identifier: 124027358

GI number: 124027358

Start: 449426

End: 451009

Strand: Direct

Name: tgtA [H]

Synonym: Hbut_0469

Alternate gene names: 124027358

Gene position: 449426-451009 (Clockwise)

Preceding gene: 124027357

Following gene: 124027363

Centisome position: 26.96

GC content: 50.32

Gene sequence:

>1584_bases
TTGAAGCGCGAGATAGGAGTTTTTGAAATCCGCGAAAAAGACCTAGCGGGAAGAATAGCTAGACTCTATACTCCACACGG
CGTACTCGAAACTCCTGCACTACTGCCAGTGATAGACCCGGTTAGGCAAGAGGTACCACTGGTCGAGATCAAGAGGCTCG
GCTTTAACGCCGTGATAACAAACGCCTATCTACTCTGGAAACGGCTTGGCGAGCAAGCGGCAGAGAAGGGCGTACATGGA
GTCTTAGGCTTTGAAGGCATAGTAATGACTGACTCTGGTGCCTACCAGCTACTCGAATACGGCCATGTGGAGGTTGAGCC
AAGAGAAATAGTTGAGTACGAGAAGAAGATAGGTAGCGATATTGCTGTAATCCTGGACGTACCAACGGGGAATACGAGGG
ATAGGAAACAAGCCGAAGAGAGTGTTAGGGAGACACTTAGACGCGCCGAGGAGGCGCAAAGCTACATAGATCCCGATAAG
AGAATATGGACCCTACCCATCCAGGGCGGCCCCTTCATAGACCTTGTCCGCGAAAGCGCGGAGAAAGCCAGGAAGCTCAA
GGACTACAAGCTATACGCCCTTGGGAGCCCAACGGTTCTCCTCGAGAGGTATGAATACAGTACGCTACTAGACATGGTAT
TTACAGCAAGGTTTAGTCTACCAGTTACAAGGCCGCTACACCTCTTCGGAGCAGGACACCCAATGATAATACCTTTCGCA
GTCGCGCTGGGCGTAGACATGTTCGACTCAGCATCTTACATCCTCTACGCGAGGGATAACCGGTACATGACCCTAACAAA
GACCTATCGGCTAGAAAACCTAGAGTATTTCCCCTGCAGCTGCCCTGTGTGCAGTAGGTATACACCACAAGAGTTGAGAG
AAATGCCGCGCGATGAGAGGGTAAAGTTGCTCGCCTTACACAACCTATACGTACTCCGACAAGCAATAAACGAGGTTAAG
ACGGCAATCCGTGAGGGCAGGCTGTGGGAGCTGCTGGAGGAAAGGAGTAGAGCCCACCCATCCCTAGCAACAGCCTTCGC
AAAATTCAGACAGTACATCGAGAAGATAGAGCAGCTAGCACCACGTGTAAAGACAGGGTTTGTAAGAGGCCTACTCCTTT
ACGGTTCTGAAAGCCTCTACAACCCTAGAGTAGTAGCCTATAGGCGCAGCATAAGAATAAACTACAAGCCGCGCATAAGG
GGAGAGCTAAAGCTAATACCTGTATCACCGTTCGAGAAGCCATTCACGTCATCAAAGCTGTACCGCAGTATCAAAAAAGG
CAGACGAGACATCCACGTGGTAGGATACATGTTGTACCTCGGCCCTATACCAGAGGAGCTGGCTGAGACCTATCCTGGCT
CACAGTTTGAGGTAAACCTAACACCATACTGGGAGGTTGTTGAAGAAACCGCAAGGAGCATAGCAGAGTACGTGGAGGCA
AACAAGGCCAACTATACAAGCATAGAGATATACTACTGTCAGGGCGATGGGCTCAAGTGGAGTAGGCCCGTTGCGGAGAA
AACAACGGAGCTACTAAGGAAAAGAGGTGTAGAAGTTAAGCTGGCTGAGCTTGGAGAGTGCTAA

Upstream 100 bases:

>100_bases
TCACAGCAAACGGCGTAGAAGGGGCTGGCACTCTCGCCGCAAAGATTAGCTACCTTCTGAAGGAGTATCTTGAGGCTAAG
AGGAGGGGCGAGAAGCCCGC

Downstream 100 bases:

>100_bases
ACAGCTTACATGTAGGCACGCTCGCTGCTCGGTGGAATAAGCGCCTCTTTCTGCTGCTGCTCCATCAACACTATCATTTC
CTCAATCTTGCGAGCCTCTT

Product: 7-cyano-7-deazaguanine tRNA-ribosyltransferase

Products: tRNAqueuine; guanine [C]

Alternate protein names: Archaeal tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 527; Mature: 527

Protein sequence:

>527_residues
MKREIGVFEIREKDLAGRIARLYTPHGVLETPALLPVIDPVRQEVPLVEIKRLGFNAVITNAYLLWKRLGEQAAEKGVHG
VLGFEGIVMTDSGAYQLLEYGHVEVEPREIVEYEKKIGSDIAVILDVPTGNTRDRKQAEESVRETLRRAEEAQSYIDPDK
RIWTLPIQGGPFIDLVRESAEKARKLKDYKLYALGSPTVLLERYEYSTLLDMVFTARFSLPVTRPLHLFGAGHPMIIPFA
VALGVDMFDSASYILYARDNRYMTLTKTYRLENLEYFPCSCPVCSRYTPQELREMPRDERVKLLALHNLYVLRQAINEVK
TAIREGRLWELLEERSRAHPSLATAFAKFRQYIEKIEQLAPRVKTGFVRGLLLYGSESLYNPRVVAYRRSIRINYKPRIR
GELKLIPVSPFEKPFTSSKLYRSIKKGRRDIHVVGYMLYLGPIPEELAETYPGSQFEVNLTPYWEVVEETARSIAEYVEA
NKANYTSIEIYYCQGDGLKWSRPVAEKTTELLRKRGVEVKLAELGEC

Sequences:

>Translated_527_residues
MKREIGVFEIREKDLAGRIARLYTPHGVLETPALLPVIDPVRQEVPLVEIKRLGFNAVITNAYLLWKRLGEQAAEKGVHG
VLGFEGIVMTDSGAYQLLEYGHVEVEPREIVEYEKKIGSDIAVILDVPTGNTRDRKQAEESVRETLRRAEEAQSYIDPDK
RIWTLPIQGGPFIDLVRESAEKARKLKDYKLYALGSPTVLLERYEYSTLLDMVFTARFSLPVTRPLHLFGAGHPMIIPFA
VALGVDMFDSASYILYARDNRYMTLTKTYRLENLEYFPCSCPVCSRYTPQELREMPRDERVKLLALHNLYVLRQAINEVK
TAIREGRLWELLEERSRAHPSLATAFAKFRQYIEKIEQLAPRVKTGFVRGLLLYGSESLYNPRVVAYRRSIRINYKPRIR
GELKLIPVSPFEKPFTSSKLYRSIKKGRRDIHVVGYMLYLGPIPEELAETYPGSQFEVNLTPYWEVVEETARSIAEYVEA
NKANYTSIEIYYCQGDGLKWSRPVAEKTTELLRKRGVEVKLAELGEC
>Mature_527_residues
MKREIGVFEIREKDLAGRIARLYTPHGVLETPALLPVIDPVRQEVPLVEIKRLGFNAVITNAYLLWKRLGEQAAEKGVHG
VLGFEGIVMTDSGAYQLLEYGHVEVEPREIVEYEKKIGSDIAVILDVPTGNTRDRKQAEESVRETLRRAEEAQSYIDPDK
RIWTLPIQGGPFIDLVRESAEKARKLKDYKLYALGSPTVLLERYEYSTLLDMVFTARFSLPVTRPLHLFGAGHPMIIPFA
VALGVDMFDSASYILYARDNRYMTLTKTYRLENLEYFPCSCPVCSRYTPQELREMPRDERVKLLALHNLYVLRQAINEVK
TAIREGRLWELLEERSRAHPSLATAFAKFRQYIEKIEQLAPRVKTGFVRGLLLYGSESLYNPRVVAYRRSIRINYKPRIR
GELKLIPVSPFEKPFTSSKLYRSIKKGRRDIHVVGYMLYLGPIPEELAETYPGSQFEVNLTPYWEVVEETARSIAEYVEA
NKANYTSIEIYYCQGDGLKWSRPVAEKTTELLRKRGVEVKLAELGEC

Specific function: Exchanges the guanine residue with 7-cyano-7- deazaguanine (preQ0) at position 15 in the dihydrouridine loop (D- loop) of archaeal tRNAs [H]

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the archaeosine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=351, Percent_Identity=25.0712250712251, Blast_Score=102, Evalue=7e-22,
Organism=Escherichia coli, GI1786607, Length=357, Percent_Identity=25.4901960784314, Blast_Score=107, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17544680, Length=355, Percent_Identity=21.1267605633803, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI19920510, Length=335, Percent_Identity=25.0746268656716, Blast_Score=86, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: 2.4.2.29 [C]

Molecular weight: Translated: 60582; Mature: 60582

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKREIGVFEIREKDLAGRIARLYTPHGVLETPALLPVIDPVRQEVPLVEIKRLGFNAVIT
CCCCCCEEEECCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHCCCHHHHH
NAYLLWKRLGEQAAEKGVHGVLGFEGIVMTDSGAYQLLEYGHVEVEPREIVEYEKKIGSD
HHHHHHHHHHHHHHHCCCCEEECCCEEEEECCCHHHHHHCCCCEECHHHHHHHHHHCCCC
IAVILDVPTGNTRDRKQAEESVRETLRRAEEAQSYIDPDKRIWTLPIQGGPFIDLVRESA
EEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHH
EKARKLKDYKLYALGSPTVLLERYEYSTLLDMVFTARFSLPVTRPLHLFGAGHPMIIPFA
HHHHHHHCEEEEEECCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCHHEECCCCCHHHHHH
VALGVDMFDSASYILYARDNRYMTLTKTYRLENLEYFPCSCPVCSRYTPQELREMPRDER
HHHCCHHCCCCCEEEEEECCCEEEEEEEEEECCCEECCCCCCCCCCCCHHHHHHCCCCCC
VKLLALHNLYVLRQAINEVKTAIREGRLWELLEERSRAHPSLATAFAKFRQYIEKIEQLA
EEEHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
PRVKTGFVRGLLLYGSESLYNPRVVAYRRSIRINYKPRIRGELKLIPVSPFEKPFTSSKL
HHHHHHHHHHHHEECCCCCCCCEEEEEEEEEEEEECCCCCCEEEEEECCCCCCCCCHHHH
YRSIKKGRRDIHVVGYMLYLGPIPEELAETYPGSQFEVNLTPYWEVVEETARSIAEYVEA
HHHHHHCCCCEEHEEEEEHHCCCCHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHC
NKANYTSIEIYYCQGDGLKWSRPVAEKTTELLRKRGVEVKLAELGEC
CCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCC
>Mature Secondary Structure
MKREIGVFEIREKDLAGRIARLYTPHGVLETPALLPVIDPVRQEVPLVEIKRLGFNAVIT
CCCCCCEEEECCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHCCCHHHHH
NAYLLWKRLGEQAAEKGVHGVLGFEGIVMTDSGAYQLLEYGHVEVEPREIVEYEKKIGSD
HHHHHHHHHHHHHHHCCCCEEECCCEEEEECCCHHHHHHCCCCEECHHHHHHHHHHCCCC
IAVILDVPTGNTRDRKQAEESVRETLRRAEEAQSYIDPDKRIWTLPIQGGPFIDLVRESA
EEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHH
EKARKLKDYKLYALGSPTVLLERYEYSTLLDMVFTARFSLPVTRPLHLFGAGHPMIIPFA
HHHHHHHCEEEEEECCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCHHEECCCCCHHHHHH
VALGVDMFDSASYILYARDNRYMTLTKTYRLENLEYFPCSCPVCSRYTPQELREMPRDER
HHHCCHHCCCCCEEEEEECCCEEEEEEEEEECCCEECCCCCCCCCCCCHHHHHHCCCCCC
VKLLALHNLYVLRQAINEVKTAIREGRLWELLEERSRAHPSLATAFAKFRQYIEKIEQLA
EEEHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
PRVKTGFVRGLLLYGSESLYNPRVVAYRRSIRINYKPRIRGELKLIPVSPFEKPFTSSKL
HHHHHHHHHHHHEECCCCCCCCEEEEEEEEEEEEECCCCCCEEEEEECCCCCCCCCHHHH
YRSIKKGRRDIHVVGYMLYLGPIPEELAETYPGSQFEVNLTPYWEVVEETARSIAEYVEA
HHHHHHCCCCEEHEEEEEHHCCCCHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHC
NKANYTSIEIYYCQGDGLKWSRPVAEKTTELLRKRGVEVKLAELGEC
CCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Zn; Mg [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.000014 {7-(aminomethyl)-7-deazaguanine}} 0.00006 {guanine}} 0.000053 {guanine}} [C]

Substrates: tRNAguanine; queuine [C]

Specific reaction: tRNAguanine + queuine = tRNAqueuine + guanine [C]

General reaction: Pentosyl group transfer [C]

Inhibitor: Bisimidoester; Dimethyl suberimidate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]