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The map label for this gene is proC [H]

Identifier: 124027225

GI number: 124027225

Start: 320038

End: 320781

Strand: Reverse

Name: proC [H]

Synonym: Hbut_0328

Alternate gene names: 124027225

Gene position: 320781-320038 (Counterclockwise)

Preceding gene: 124027227

Following gene: 124027223

Centisome position: 19.24

GC content: 61.02

Gene sequence:

>744_bases
GTGGTTGCGACGGCTCGCAGCCAGGAGAGGCTTGTGTTGCTCCGTGAGGCTGGGTTGGAGGCTACCAGTTGTAACCGGTG
TGCTGTCGAGAGTGCAGACCTGGTGTTCATAAGTGTCAAGCCCTACCACTTCCCCATTGTTGCCCGTGACATAGGGGGTA
TTGTTGCTGGCAAGCCGGTCGTCTCTGTGATGGCTGGTGTGCCTGTTTCGCTCCTACAGAAGGTGCTGAAGGGTGCTGAG
GTCTACCGCGCTATGCCCAACTTGAACATACGGGTTGGGAGGAGCCTCACAGCCCTCTACTCTCCGCCCAACGCGAGGCA
CTACGGGCTCGTGAGGAGGGCCCTTTCCTGCTTTGGCACGGTGTACGATGTCCCGGAGGAGTTCATTGATGCATGGACGG
CGGTGGCCGGGTCGGGGCCAGCGCTGGTCGCAGAGTTTCTCGACGCGCTCGTGCTGGCAGCGCTGGCTGTTGGGCTTCCG
AGGAGTATTGCCCGGAGGGTGGCGGCGGAGCTTCTCGAGTCTACCGCGAGGTACTTGCTCGAGAACCCTGATGTGCACCC
CGGCGTTCTCAGGGACGAGGTTATAACGCCGGCGGGTACTACGATAGCTGCTATTCGGACCCTCGAGAGCAGGGGGTTCA
AGTCGGCTGTGATCGATGCCGTTCGTGATGCTACGTTGAGGGCGGGCGAGATAGCGCGGGAGATAGCGTCCATGCTGGAT
AATAGCCTGGGCGGCAGCGACTAA

Upstream 100 bases:

>100_bases
TGCCCGGGGATGGGTCTTCCAGGGTGTTAGGGTAGCTGTTATTGGTGCTGGTAAGGTTGGTTTTGCTATTGCTCGTTCTC
TGCGTGGCTGTGGCGCGTCT

Downstream 100 bases:

>100_bases
TAGTAGTCTCCCAGCAGCCAGATGTTCCCGATGTAGACCTCTTTCACGCCTGCCTCGAGCACGGCTTTTCTCGCCTCGGC
GGCGTGTCTGCGGCTGGTTG

Product: pyrroline-5-carboxylate reductase

Products: NA

Alternate protein names: P5C reductase; P5CR [H]

Number of amino acids: Translated: 247; Mature: 247

Protein sequence:

>247_residues
MVATARSQERLVLLREAGLEATSCNRCAVESADLVFISVKPYHFPIVARDIGGIVAGKPVVSVMAGVPVSLLQKVLKGAE
VYRAMPNLNIRVGRSLTALYSPPNARHYGLVRRALSCFGTVYDVPEEFIDAWTAVAGSGPALVAEFLDALVLAALAVGLP
RSIARRVAAELLESTARYLLENPDVHPGVLRDEVITPAGTTIAAIRTLESRGFKSAVIDAVRDATLRAGEIAREIASMLD
NSLGGSD

Sequences:

>Translated_247_residues
MVATARSQERLVLLREAGLEATSCNRCAVESADLVFISVKPYHFPIVARDIGGIVAGKPVVSVMAGVPVSLLQKVLKGAE
VYRAMPNLNIRVGRSLTALYSPPNARHYGLVRRALSCFGTVYDVPEEFIDAWTAVAGSGPALVAEFLDALVLAALAVGLP
RSIARRVAAELLESTARYLLENPDVHPGVLRDEVITPAGTTIAAIRTLESRGFKSAVIDAVRDATLRAGEIAREIASMLD
NSLGGSD
>Mature_247_residues
MVATARSQERLVLLREAGLEATSCNRCAVESADLVFISVKPYHFPIVARDIGGIVAGKPVVSVMAGVPVSLLQKVLKGAE
VYRAMPNLNIRVGRSLTALYSPPNARHYGLVRRALSCFGTVYDVPEEFIDAWTAVAGSGPALVAEFLDALVLAALAVGLP
RSIARRVAAELLESTARYLLENPDVHPGVLRDEVITPAGTTIAAIRTLESRGFKSAVIDAVRDATLRAGEIAREIASMLD
NSLGGSD

Specific function: Proline biosynthesis; third (last) step. [C]

COG id: COG0345

COG function: function code E; Pyrroline-5-carboxylate reductase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyrroline-5-carboxylate reductase family [H]

Homologues:

Organism=Homo sapiens, GI24797097, Length=235, Percent_Identity=36.5957446808511, Blast_Score=131, Evalue=7e-31,
Organism=Homo sapiens, GI24797095, Length=235, Percent_Identity=36.5957446808511, Blast_Score=131, Evalue=7e-31,
Organism=Homo sapiens, GI198041662, Length=241, Percent_Identity=34.8547717842324, Blast_Score=127, Evalue=7e-30,
Organism=Homo sapiens, GI21361454, Length=235, Percent_Identity=35.7446808510638, Blast_Score=125, Evalue=4e-29,
Organism=Escherichia coli, GI1786585, Length=227, Percent_Identity=32.15859030837, Blast_Score=107, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI17569021, Length=215, Percent_Identity=35.3488372093023, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17540664, Length=253, Percent_Identity=31.6205533596838, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6320861, Length=212, Percent_Identity=32.0754716981132, Blast_Score=111, Evalue=9e-26,
Organism=Drosophila melanogaster, GI21358587, Length=237, Percent_Identity=35.8649789029536, Blast_Score=125, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24648116, Length=243, Percent_Identity=35.3909465020576, Blast_Score=123, Evalue=9e-29,
Organism=Drosophila melanogaster, GI24647700, Length=170, Percent_Identity=37.6470588235294, Blast_Score=97, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008927
- InterPro:   IPR016040
- InterPro:   IPR004455
- InterPro:   IPR000304 [H]

Pfam domain/function: PF03807 F420_oxidored [H]

EC number: =1.5.1.2 [H]

Molecular weight: Translated: 26233; Mature: 26233

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVATARSQERLVLLREAGLEATSCNRCAVESADLVFISVKPYHFPIVARDIGGIVAGKPV
CCCCCCCCHHHHHHHHCCCCCCCCCHHCCCCCCEEEEEECCCCCEEHHHHHCCHHCCCHH
VSVMAGVPVSLLQKVLKGAEVYRAMPNLNIRVGRSLTALYSPPNARHYGLVRRALSCFGT
HHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEECCCCCHHHHHHHHHHHHHHH
VYDVPEEFIDAWTAVAGSGPALVAEFLDALVLAALAVGLPRSIARRVAAELLESTARYLL
HHHCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
ENPDVHPGVLRDEVITPAGTTIAAIRTLESRGFKSAVIDAVRDATLRAGEIAREIASMLD
CCCCCCCCCHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NSLGGSD
HCCCCCC
>Mature Secondary Structure
MVATARSQERLVLLREAGLEATSCNRCAVESADLVFISVKPYHFPIVARDIGGIVAGKPV
CCCCCCCCHHHHHHHHCCCCCCCCCHHCCCCCCEEEEEECCCCCEEHHHHHCCHHCCCHH
VSVMAGVPVSLLQKVLKGAEVYRAMPNLNIRVGRSLTALYSPPNARHYGLVRRALSCFGT
HHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEECCCCCHHHHHHHHHHHHHHH
VYDVPEEFIDAWTAVAGSGPALVAEFLDALVLAALAVGLPRSIARRVAAELLESTARYLL
HHHCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
ENPDVHPGVLRDEVITPAGTTIAAIRTLESRGFKSAVIDAVRDATLRAGEIAREIASMLD
CCCCCCCCCHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NSLGGSD
HCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]