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The map label for this gene is thiB [H]

Identifier: 124027223

GI number: 124027223

Start: 316364

End: 317608

Strand: Reverse

Name: thiB [H]

Synonym: Hbut_0326

Alternate gene names: 124027223

Gene position: 317608-316364 (Counterclockwise)

Preceding gene: 124027225

Following gene: 124027222

Centisome position: 19.05

GC content: 57.27

Gene sequence:

>1245_bases
GTGACGGGTAGGGCGGTCTACTTCCTAGCGGGGGTTCTGGCAATTCTCCTTGTGGCTGCGCTCGCGGTCTACGTGTCCTA
TCAGCGTAGTGGCGTTGGCGAGGGAACGAGCAATAGCAAAACCAACCTTACCAGCACCAATAACAGCTACCCTAACACCC
TGGAAGAGCCATCCCCGGGCACCACTGTAAACTCACGCCCGATACGACAAGCTATAACCCCTAAGCCGGTTAAGCTTGTT
GTCTACACTTATGATGATTTTATGGCGTGGGGTGAGGATGAGGAGCTTTTCGACAAGCTTGTCCAGCGCTTCGAGAACGA
GACTGGGATAGACGTGGTGATATACCGGTTTGACGGTGCTAGGCAGATGGTTACCCAGGTGCTGAACGAGCTGGACCAGG
GAGTGGAGACGGCAGACGTTGTGATAGGTATTGATCCCATCTTGCTGCAGGAGCTGAAGAAGCATAACGCGGTTGTGTGC
TATCTGTCGCCCAGTGCTCCCCTGGAGGTTGCCAATGCGCTTGACCCGGATCATTGTGCGACGCCGATAGACTATGGATT
GATAGCACTGGTCTATGACCCATCCCGGCTGACCGAGGAGGAGCTAGCTATGCTCCGCGACGGTGTAACCCTCGACGAGC
TAGTGAAGCTTGCACCGCGTATTGTGGGCGAGGACCCGACCAAGAGTAGTACGGGGCTTAACTTCCTCCTCTACACTATG
GCCCTATCGAGGCTTGAGGGGAGGGACTGGAAGGAGCTATGGCGCCAGCTCGTAGGGAATGGCATGATGATCGCGCCTAG
CTGGGGTAGTGCGTACGACGAGTTCTTGAGGGAGGGCAGTCCCAGAGCCATAGTTGTAAGCTATGGCACCGACCCTGCAT
ACAGCGCCTGGTACAGCGCCAAGGAGGGTGGCGCTGAGGAGCCCTCGGTTAACGCCACGGTGCTAGCAGCGGGTGGGAAG
AGGCTCGGCTGGATGCAGGTCGAGGGCGTCGCGATTATACGTGGTGCAGAGCTTGATGCTGCCAAGAAGTTTGTGGACTG
GCTGCTCAGCGAGGAGGTGCAGCGCGAGATACCGGCAAGCCAGTGGATGTTCCCAGCGGTGGATGTTAGCCTGCCGAGCT
TCTACAAGTACGCGCTTACGGCCAAAGACGTGGACGGCATGGCTAACAGTGTTCTACCGCCAGACGAGATATACGAGTCC
CTCGAGGTGTGGCTGAAGGATTGGCTCAGCATAGCTGGGGGCTAG

Upstream 100 bases:

>100_bases
GTTGATGTTGTTAGGGAGGTACTTCTGGCCCACTTCTTGCCTAGGAGAGGGTTATTGACCCCCCTATCACTTTGTTATTG
CCAGCTTGGGGTGGCTAGGT

Downstream 100 bases:

>100_bases
CCTGGAACCCCCTCGGCCGCCTATGGTGGCTACTAGCGCTAGCCGCCTGGCTAACCGTCGCCGTCTATACAGCCCCTATT
TTCATCTCCCTAGCCTACTC

Product: thiamine-binding, periplasmic protein

Products: ADP; phosphate; thiamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 414; Mature: 413

Protein sequence:

>414_residues
MTGRAVYFLAGVLAILLVAALAVYVSYQRSGVGEGTSNSKTNLTSTNNSYPNTLEEPSPGTTVNSRPIRQAITPKPVKLV
VYTYDDFMAWGEDEELFDKLVQRFENETGIDVVIYRFDGARQMVTQVLNELDQGVETADVVIGIDPILLQELKKHNAVVC
YLSPSAPLEVANALDPDHCATPIDYGLIALVYDPSRLTEEELAMLRDGVTLDELVKLAPRIVGEDPTKSSTGLNFLLYTM
ALSRLEGRDWKELWRQLVGNGMMIAPSWGSAYDEFLREGSPRAIVVSYGTDPAYSAWYSAKEGGAEEPSVNATVLAAGGK
RLGWMQVEGVAIIRGAELDAAKKFVDWLLSEEVQREIPASQWMFPAVDVSLPSFYKYALTAKDVDGMANSVLPPDEIYES
LEVWLKDWLSIAGG

Sequences:

>Translated_414_residues
MTGRAVYFLAGVLAILLVAALAVYVSYQRSGVGEGTSNSKTNLTSTNNSYPNTLEEPSPGTTVNSRPIRQAITPKPVKLV
VYTYDDFMAWGEDEELFDKLVQRFENETGIDVVIYRFDGARQMVTQVLNELDQGVETADVVIGIDPILLQELKKHNAVVC
YLSPSAPLEVANALDPDHCATPIDYGLIALVYDPSRLTEEELAMLRDGVTLDELVKLAPRIVGEDPTKSSTGLNFLLYTM
ALSRLEGRDWKELWRQLVGNGMMIAPSWGSAYDEFLREGSPRAIVVSYGTDPAYSAWYSAKEGGAEEPSVNATVLAAGGK
RLGWMQVEGVAIIRGAELDAAKKFVDWLLSEEVQREIPASQWMFPAVDVSLPSFYKYALTAKDVDGMANSVLPPDEIYES
LEVWLKDWLSIAGG
>Mature_413_residues
TGRAVYFLAGVLAILLVAALAVYVSYQRSGVGEGTSNSKTNLTSTNNSYPNTLEEPSPGTTVNSRPIRQAITPKPVKLVV
YTYDDFMAWGEDEELFDKLVQRFENETGIDVVIYRFDGARQMVTQVLNELDQGVETADVVIGIDPILLQELKKHNAVVCY
LSPSAPLEVANALDPDHCATPIDYGLIALVYDPSRLTEEELAMLRDGVTLDELVKLAPRIVGEDPTKSSTGLNFLLYTMA
LSRLEGRDWKELWRQLVGNGMMIAPSWGSAYDEFLREGSPRAIVVSYGTDPAYSAWYSAKEGGAEEPSVNATVLAAGGKR
LGWMQVEGVAIIRGAELDAAKKFVDWLLSEEVQREIPASQWMFPAVDVSLPSFYKYALTAKDVDGMANSVLPPDEIYESL
EVWLKDWLSIAGG

Specific function: Part of the ABC transporter complex thiBPQ involved in thiamine import [H]

COG id: COG4143

COG function: function code H; ABC-type thiamine transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

Organism=Escherichia coli, GI1786255, Length=339, Percent_Identity=27.1386430678466, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR005948
- InterPro:   IPR005967 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 45507; Mature: 45376

Theoretical pI: Translated: 4.23; Mature: 4.23

Prosite motif: PS01037 SBP_BACTERIAL_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGRAVYFLAGVLAILLVAALAVYVSYQRSGVGEGTSNSKTNLTSTNNSYPNTLEEPSPG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
TTVNSRPIRQAITPKPVKLVVYTYDDFMAWGEDEELFDKLVQRFENETGIDVVIYRFDGA
CCCCCCCHHHCCCCCCEEEEEEEEHHHHHCCCCHHHHHHHHHHHCCCCCCEEEEEEECCH
RQMVTQVLNELDQGVETADVVIGIDPILLQELKKHNAVVCYLSPSAPLEVANALDPDHCA
HHHHHHHHHHHHCCCCHHHEEEECCHHHHHHHHHCCCEEEEECCCCCHHHHHCCCCCCCC
TPIDYGLIALVYDPSRLTEEELAMLRDGVTLDELVKLAPRIVGEDPTKSSTGLNFLLYTM
CCCCCCEEEEEECCHHCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
ALSRLEGRDWKELWRQLVGNGMMIAPSWGSAYDEFLREGSPRAIVVSYGTDPAYSAWYSA
HHHHHCCCCHHHHHHHHHCCCEEEECCCCHHHHHHHHCCCCCEEEEECCCCCCHHHHHCC
KEGGAEEPSVNATVLAAGGKRLGWMQVEGVAIIRGAELDAAKKFVDWLLSEEVQREIPAS
CCCCCCCCCCCEEEEECCCCEECEEEECCEEEEECCCHHHHHHHHHHHHHHHHHHHCCHH
QWMFPAVDVSLPSFYKYALTAKDVDGMANSVLPPDEIYESLEVWLKDWLSIAGG
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TGRAVYFLAGVLAILLVAALAVYVSYQRSGVGEGTSNSKTNLTSTNNSYPNTLEEPSPG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
TTVNSRPIRQAITPKPVKLVVYTYDDFMAWGEDEELFDKLVQRFENETGIDVVIYRFDGA
CCCCCCCHHHCCCCCCEEEEEEEEHHHHHCCCCHHHHHHHHHHHCCCCCCEEEEEEECCH
RQMVTQVLNELDQGVETADVVIGIDPILLQELKKHNAVVCYLSPSAPLEVANALDPDHCA
HHHHHHHHHHHHCCCCHHHEEEECCHHHHHHHHHCCCEEEEECCCCCHHHHHCCCCCCCC
TPIDYGLIALVYDPSRLTEEELAMLRDGVTLDELVKLAPRIVGEDPTKSSTGLNFLLYTM
CCCCCCEEEEEECCHHCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
ALSRLEGRDWKELWRQLVGNGMMIAPSWGSAYDEFLREGSPRAIVVSYGTDPAYSAWYSA
HHHHHCCCCHHHHHHHHHCCCEEEECCCCHHHHHHHHCCCCCEEEEECCCCCCHHHHHCC
KEGGAEEPSVNATVLAAGGKRLGWMQVEGVAIIRGAELDAAKKFVDWLLSEEVQREIPAS
CCCCCCCCCCCEEEEECCCCEECEEEECCEEEEECCCHHHHHHHHHHHHHHHHHHHCCHH
QWMFPAVDVSLPSFYKYALTAKDVDGMANSVLPPDEIYESLEVWLKDWLSIAGG
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; thiamine [Periplasm]; H2O [C]

Specific reaction: ATP + thiamine [Periplasm] + H2O = ADP + phosphate + thiamine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA