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The map label for this gene is thiC [H]

Identifier: 124027186

GI number: 124027186

Start: 274393

End: 275688

Strand: Direct

Name: thiC [H]

Synonym: Hbut_0289

Alternate gene names: 124027186

Gene position: 274393-275688 (Clockwise)

Preceding gene: 124027184

Following gene: 124027187

Centisome position: 16.46

GC content: 54.86

Gene sequence:

>1296_bases
TTGGCGAAGACGGTTATGCTTGAGGCTCGTAGTGGGTCGGTGCCGGAGGAAGTTCTGGAGGTTGCTAGGACTGAGGGTGT
GGATCCGAACAAGCTCCGGGTTAGGCTTGCTGAAGGCAGGGTGATTATTGCGAGGAATATCAAGCGGCAGGGCAGGATTA
GGCTTGTTGGTGTTGGTGAGGGGCTTACAACGAAGGTTAACGTTAACATTGGTGTTAGCACCCATGTGAACGATATTGAA
ATGGAGAAGGAGAAGGCTAGGATAGCGGTTCAATATGGTGCCGACACGATTATGGATTTGAGTGTTGGTGAGAACCTCGA
CGAGGTTAGAAGAATCCTTATGGAGGCTTCGGAGCCTCTACCATTTGGCACTGTGCCAACGTACCAGGCTTATATTGAGG
GCGTGAGAAAGCATGGGGGGATGCCCGACGAGGACTTCTTCCTCCAGGTTCTCGAGCGCCATCTACGGGACGGCGTGGAC
TTTATGACAATCCATGCCGGCATTACTAGGGAGCTGGCGGAGAGGGCGCTTAAGAGTAACCGTATACAGCCCATAGTTAG
CCGTGGCGGTGCCCTGCTGGCGGCATGGATGATGGAAACCGGCAGCGAGAACCCCTACTACCGGAACTGGGACTACATAC
TAGAGCTGTTTGCAGAGTACGATGCCGTGATAAGTCTTGGTGATGCGCTGCGCCCTGGCGCTATAGCTGATGCGCTAGAC
GAGCTTCAGATCCTGGAGCTGATCAATAATGCACGGCTCGTGGAGAGGGCTCGGGAAAAGGGTGTACAGGTTATGGTTGA
GGGACCTGGCCACGTGCCGATAAACAAAATCGTCGCTGATATAAGGCTTATGAAGAGCCTCACCAGGGGTGCCCCCTACT
ACGTGCTTGGCCCGCTTGTGACCGATATAGGGCTCGGCTACGACCACATAGCGGCAGCTATAGGCGCTGCAATAGCTGCT
GCCGAGGGTGCCGACCTCATCTGCTACCTGACACCCGCCGAGCACCTAAGCCTCCCAAACCCGGAGCAGGTTAAGGAGGG
CCTCATAGCAGCGAGAATAGCTGCGCACGCTGGTGACATAGTCAAGCTTGGCAGGAGGGTTATGGAGCCCGACATTAAGA
TGAGCATTCACCGTGCAAGGCTCGAGTGGAATAAGATGTTCAAGCTCTCGCTGGACCCGGAGAGGGCTAGGAGGATCTAT
AGCCAGTACGGCGTGCAGCTCCCATCGTGCACTATGTGTGGCAGCTACTGTGCTTTTCTCATCCTCAACAAGTACATGGA
GAAGACCCGGAGGTGA

Upstream 100 bases:

>100_bases
CCGGACTCAAGCTTCAGCCTGCTCCAGGCAATTATTACAGTGTTATGTATTGCTCTATAAACCCGTTCTAGATGCAGGGT
TCCTCACGGTGTGGTGCTTC

Downstream 100 bases:

>100_bases
TGCTGGATGACTGGAGAGCATGAGGAGAGGGCGGGTTATCCCTGGGTTTGGAGCACCTGGGTTAAGGCACCGATACTGAG
GAATAGGGATCTCCTCATAG

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC [H]

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MAKTVMLEARSGSVPEEVLEVARTEGVDPNKLRVRLAEGRVIIARNIKRQGRIRLVGVGEGLTTKVNVNIGVSTHVNDIE
MEKEKARIAVQYGADTIMDLSVGENLDEVRRILMEASEPLPFGTVPTYQAYIEGVRKHGGMPDEDFFLQVLERHLRDGVD
FMTIHAGITRELAERALKSNRIQPIVSRGGALLAAWMMETGSENPYYRNWDYILELFAEYDAVISLGDALRPGAIADALD
ELQILELINNARLVERAREKGVQVMVEGPGHVPINKIVADIRLMKSLTRGAPYYVLGPLVTDIGLGYDHIAAAIGAAIAA
AEGADLICYLTPAEHLSLPNPEQVKEGLIAARIAAHAGDIVKLGRRVMEPDIKMSIHRARLEWNKMFKLSLDPERARRIY
SQYGVQLPSCTMCGSYCAFLILNKYMEKTRR

Sequences:

>Translated_431_residues
MAKTVMLEARSGSVPEEVLEVARTEGVDPNKLRVRLAEGRVIIARNIKRQGRIRLVGVGEGLTTKVNVNIGVSTHVNDIE
MEKEKARIAVQYGADTIMDLSVGENLDEVRRILMEASEPLPFGTVPTYQAYIEGVRKHGGMPDEDFFLQVLERHLRDGVD
FMTIHAGITRELAERALKSNRIQPIVSRGGALLAAWMMETGSENPYYRNWDYILELFAEYDAVISLGDALRPGAIADALD
ELQILELINNARLVERAREKGVQVMVEGPGHVPINKIVADIRLMKSLTRGAPYYVLGPLVTDIGLGYDHIAAAIGAAIAA
AEGADLICYLTPAEHLSLPNPEQVKEGLIAARIAAHAGDIVKLGRRVMEPDIKMSIHRARLEWNKMFKLSLDPERARRIY
SQYGVQLPSCTMCGSYCAFLILNKYMEKTRR
>Mature_430_residues
AKTVMLEARSGSVPEEVLEVARTEGVDPNKLRVRLAEGRVIIARNIKRQGRIRLVGVGEGLTTKVNVNIGVSTHVNDIEM
EKEKARIAVQYGADTIMDLSVGENLDEVRRILMEASEPLPFGTVPTYQAYIEGVRKHGGMPDEDFFLQVLERHLRDGVDF
MTIHAGITRELAERALKSNRIQPIVSRGGALLAAWMMETGSENPYYRNWDYILELFAEYDAVISLGDALRPGAIADALDE
LQILELINNARLVERAREKGVQVMVEGPGHVPINKIVADIRLMKSLTRGAPYYVLGPLVTDIGLGYDHIAAAIGAAIAAA
EGADLICYLTPAEHLSLPNPEQVKEGLIAARIAAHAGDIVKLGRRVMEPDIKMSIHRARLEWNKMFKLSLDPERARRIYS
QYGVQLPSCTMCGSYCAFLILNKYMEKTRR

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction [H]

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family [H]

Homologues:

Organism=Escherichia coli, GI1790427, Length=409, Percent_Identity=40.5867970660147, Blast_Score=280, Evalue=2e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002817 [H]

Pfam domain/function: PF01964 ThiC [H]

EC number: NA

Molecular weight: Translated: 47853; Mature: 47722

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKTVMLEARSGSVPEEVLEVARTEGVDPNKLRVRLAEGRVIIARNIKRQGRIRLVGVGE
CCCEEEEECCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCEEEEECCCCCCCEEEEEECC
GLTTKVNVNIGVSTHVNDIEMEKEKARIAVQYGADTIMDLSVGENLDEVRRILMEASEPL
CCEEEEEEEEECCCCCCHHHHHHHHHEEEEEECCCEEEEECCCCCHHHHHHHHHHCCCCC
PFGTVPTYQAYIEGVRKHGGMPDEDFFLQVLERHLRDGVDFMTIHAGITRELAERALKSN
CCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCC
RIQPIVSRGGALLAAWMMETGSENPYYRNWDYILELFAEYDAVISLGDALRPGAIADALD
CCCHHHHCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
ELQILELINNARLVERAREKGVQVMVEGPGHVPINKIVADIRLMKSLTRGAPYYVLGPLV
HHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEHHHH
TDIGLGYDHIAAAIGAAIAAAEGADLICYLTPAEHLSLPNPEQVKEGLIAARIAAHAGDI
HHHCCCHHHHHHHHHHHHHHCCCCCEEEEECCHHHCCCCCHHHHHHHHHHHHHHHHCCHH
VKLGRRVMEPDIKMSIHRARLEWNKMFKLSLDPERARRIYSQYGVQLPSCTMCGSYCAFL
HHHHHHHHCCCHHHHHHHHHHHHHHEEEEECCHHHHHHHHHHHCCCCCCHHHHHHHHHHH
ILNKYMEKTRR
HHHHHHHHHCC
>Mature Secondary Structure 
AKTVMLEARSGSVPEEVLEVARTEGVDPNKLRVRLAEGRVIIARNIKRQGRIRLVGVGE
CCEEEEECCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCEEEEECCCCCCCEEEEEECC
GLTTKVNVNIGVSTHVNDIEMEKEKARIAVQYGADTIMDLSVGENLDEVRRILMEASEPL
CCEEEEEEEEECCCCCCHHHHHHHHHEEEEEECCCEEEEECCCCCHHHHHHHHHHCCCCC
PFGTVPTYQAYIEGVRKHGGMPDEDFFLQVLERHLRDGVDFMTIHAGITRELAERALKSN
CCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCC
RIQPIVSRGGALLAAWMMETGSENPYYRNWDYILELFAEYDAVISLGDALRPGAIADALD
CCCHHHHCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
ELQILELINNARLVERAREKGVQVMVEGPGHVPINKIVADIRLMKSLTRGAPYYVLGPLV
HHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEHHHH
TDIGLGYDHIAAAIGAAIAAAEGADLICYLTPAEHLSLPNPEQVKEGLIAARIAAHAGDI
HHHCCCHHHHHHHHHHHHHHCCCCCEEEEECCHHHCCCCCHHHHHHHHHHHHHHHHCCHH
VKLGRRVMEPDIKMSIHRARLEWNKMFKLSLDPERARRIYSQYGVQLPSCTMCGSYCAFL
HHHHHHHHCCCHHHHHHHHHHHHHHEEEEECCHHHHHHHHHHHCCCCCCHHHHHHHHHHH
ILNKYMEKTRR
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA