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The map label for this gene is cpkA [H]

Identifier: 124027166

GI number: 124027166

Start: 255405

End: 256370

Strand: Direct

Name: cpkA [H]

Synonym: Hbut_0269

Alternate gene names: 124027166

Gene position: 255405-256370 (Clockwise)

Preceding gene: 124027164

Following gene: 124027169

Centisome position: 15.32

GC content: 59.01

Gene sequence:

>966_bases
TTGGCTTCAGCCACCCCCACCCCCAACGACCTCTACGTAATAGCACTGGGCGGCAACGCGTTCATGAAGAAGGGCGAGGG
CGTTGAGGCCCAGTGGCACAATGTGGAGAAAGCGGCTCGACAGCTGGTTGACCTCATCGAGCAGGGCTACAGGATAGTGG
TAACCCACGGTAATGGGCCCCAGGTGGGCCTCATAGTTAACTGGGTACACCACTACTACGGTCGGCCCGTGGAAAGGTTG
ACACTCGACATCGCTGGAGCCATGACGCAGGGCTGGCTGGGCTACATGCTCCAGCAAGCCATAGGTAACGAGCTGGAGCG
CCGCGGCATGCCCCGTCGCGTAGTCACGCTCGTCTCCCAGGTCCTCGTAGACCGCAACGACCCTGCATTCCAGAACCCGA
CAAAGTACGTGGGCCCATACTACTCGAGGGAGGAAGCTGAGAGGATAGCCAAGGAGACCGGCTGGGTCTTCAAGCCGGAC
CCCAGGGGTGGCTATCGCCGCGTAGTGCCGAGCCCCAAGCCGCTCCGTGTCGTGGAGATAGAGGCTGTGAAGAGGCTTAT
CGAGCAAGGCTTCATAGTGATAACAGTTGGTGGCGGTGGCATACCAGTGGTAAGCAGTGACGGTCGCCTACGTGGCGTGG
AGGCTGTGATAGACAAGGATCTGGCCTCGAGCCTCCTCGCCCAGAGCCTCAGAGCTAGAGCCCTCATAATACTCACTGAT
GTCGACTACGTCTACCTAAACTATGGTAAGCCGAACCAGCAGCCGCTAAAGTTGCTAAAGGCGTCGGAGGCCCGTAGGCT
GCTAGAGGAGGGACACTTCCCTCCAGGCAGTATGGGTCCAAAGGTGCAAGCTGCAATAGAGTTCGTAGAGGCTATGGGTG
AGGGATACTTCGCCGCCATAGGGAGCCTTGACAACGCGTTAGCAGTGGCGCGTGGCGAAAAAGGTACAAGGATTGTCCCC
GGCTAG

Upstream 100 bases:

>100_bases
TGGCTATGCGTGGTACTGTTTGGGGGTGGGAAAACCATTGGAGAACCAGTAGCCCACTATTTATAACTGGTGAATCCACT
GGAACTCCATGGTGTAGAGC

Downstream 100 bases:

>100_bases
GCGTTCCTCTGGTATAGAGGGCATAGCGCGCAGAACCAGGGCGCAGCGCTCACCTTAACCCGGTAAATCCCCTCGTCTTC
GACGACTATGTCCATGCCGA

Product: carbamate kinase

Products: NA

Alternate protein names: Carbamate kinase-like carbamoylphosphate synthase [H]

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MASATPTPNDLYVIALGGNAFMKKGEGVEAQWHNVEKAARQLVDLIEQGYRIVVTHGNGPQVGLIVNWVHHYYGRPVERL
TLDIAGAMTQGWLGYMLQQAIGNELERRGMPRRVVTLVSQVLVDRNDPAFQNPTKYVGPYYSREEAERIAKETGWVFKPD
PRGGYRRVVPSPKPLRVVEIEAVKRLIEQGFIVITVGGGGIPVVSSDGRLRGVEAVIDKDLASSLLAQSLRARALIILTD
VDYVYLNYGKPNQQPLKLLKASEARRLLEEGHFPPGSMGPKVQAAIEFVEAMGEGYFAAIGSLDNALAVARGEKGTRIVP
G

Sequences:

>Translated_321_residues
MASATPTPNDLYVIALGGNAFMKKGEGVEAQWHNVEKAARQLVDLIEQGYRIVVTHGNGPQVGLIVNWVHHYYGRPVERL
TLDIAGAMTQGWLGYMLQQAIGNELERRGMPRRVVTLVSQVLVDRNDPAFQNPTKYVGPYYSREEAERIAKETGWVFKPD
PRGGYRRVVPSPKPLRVVEIEAVKRLIEQGFIVITVGGGGIPVVSSDGRLRGVEAVIDKDLASSLLAQSLRARALIILTD
VDYVYLNYGKPNQQPLKLLKASEARRLLEEGHFPPGSMGPKVQAAIEFVEAMGEGYFAAIGSLDNALAVARGEKGTRIVP
G
>Mature_320_residues
ASATPTPNDLYVIALGGNAFMKKGEGVEAQWHNVEKAARQLVDLIEQGYRIVVTHGNGPQVGLIVNWVHHYYGRPVERLT
LDIAGAMTQGWLGYMLQQAIGNELERRGMPRRVVTLVSQVLVDRNDPAFQNPTKYVGPYYSREEAERIAKETGWVFKPDP
RGGYRRVVPSPKPLRVVEIEAVKRLIEQGFIVITVGGGGIPVVSSDGRLRGVEAVIDKDLASSLLAQSLRARALIILTDV
DYVYLNYGKPNQQPLKLLKASEARRLLEEGHFPPGSMGPKVQAAIEFVEAMGEGYFAAIGSLDNALAVARGEKGTRIVPG

Specific function: Carbamate kinase that plays a biosynthetic role in that it produces carbamoyl-phosphate [H]

COG id: COG0549

COG function: function code E; Carbamate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbamate kinase family [H]

Homologues:

Organism=Escherichia coli, GI1789238, Length=274, Percent_Identity=49.2700729927007, Blast_Score=253, Evalue=1e-68,
Organism=Escherichia coli, GI1786516, Length=318, Percent_Identity=43.7106918238994, Blast_Score=220, Evalue=8e-59,
Organism=Escherichia coli, GI1786732, Length=310, Percent_Identity=40.6451612903226, Blast_Score=199, Evalue=2e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR003964 [H]

Pfam domain/function: PF00696 AA_kinase [H]

EC number: =2.7.2.2 [H]

Molecular weight: Translated: 35179; Mature: 35048

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASATPTPNDLYVIALGGNAFMKKGEGVEAQWHNVEKAARQLVDLIEQGYRIVVTHGNGP
CCCCCCCCCCEEEEEECCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCC
QVGLIVNWVHHYYGRPVERLTLDIAGAMTQGWLGYMLQQAIGNELERRGMPRRVVTLVSQ
EEHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
VLVDRNDPAFQNPTKYVGPYYSREEAERIAKETGWVFKPDPRGGYRRVVPSPKPLRVVEI
HHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEECCCCCCCCEECCCCCCCEEEEEH
EAVKRLIEQGFIVITVGGGGIPVVSSDGRLRGVEAVIDKDLASSLLAQSLRARALIILTD
HHHHHHHHCCEEEEEECCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEEE
VDYVYLNYGKPNQQPLKLLKASEARRLLEEGHFPPGSMGPKVQAAIEFVEAMGEGYFAAI
CCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEEC
GSLDNALAVARGEKGTRIVPG
CCCCHHHHEECCCCCCCCCCC
>Mature Secondary Structure 
ASATPTPNDLYVIALGGNAFMKKGEGVEAQWHNVEKAARQLVDLIEQGYRIVVTHGNGP
CCCCCCCCCEEEEEECCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCC
QVGLIVNWVHHYYGRPVERLTLDIAGAMTQGWLGYMLQQAIGNELERRGMPRRVVTLVSQ
EEHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
VLVDRNDPAFQNPTKYVGPYYSREEAERIAKETGWVFKPDPRGGYRRVVPSPKPLRVVEI
HHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEECCCCCCCCEECCCCCCCEEEEEH
EAVKRLIEQGFIVITVGGGGIPVVSSDGRLRGVEAVIDKDLASSLLAQSLRARALIILTD
HHHHHHHHCCEEEEEECCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEEE
VDYVYLNYGKPNQQPLKLLKASEARRLLEEGHFPPGSMGPKVQAAIEFVEAMGEGYFAAI
CCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEEC
GSLDNALAVARGEKGTRIVPG
CCCCHHHHEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371756; 10860751 [H]