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The map label for this gene is yfjR [H]

Identifier: 124027090

GI number: 124027090

Start: 174731

End: 175627

Strand: Reverse

Name: yfjR [H]

Synonym: Hbut_0193

Alternate gene names: 124027090

Gene position: 175627-174731 (Counterclockwise)

Preceding gene: 124027092

Following gene: 124027081

Centisome position: 10.53

GC content: 61.87

Gene sequence:

>897_bases
TTGGAGCAGTGTGCAGCGGTTTTAGGTCTTGGCAGGATGGGCTCGGCTATTGCCGAGAGGCTACACGATAGGGGCTTCAG
ACTGGTTGTATGGAACCGTACGAGGAGCCGGGCTGAAGAGCTCGCAGCTAGGCTTGACGCGGAAGTTGCTGGGTCTCCGG
CTGGGGCTGCAGAGAAGTGTAACGCCGTACACATCGTGGTGTCTGACGATGAGGCCTCAATGAGCGTCATAGCGGGGCCC
ACGGGAATCACATCTATTAATCTAGGGGGGAGGCTCGTCTATAACCACACCACCGTAACCCCCCGCCACACTATGCTGGC
GCTAGAGCTTGTAGAGCGCGCCGGTGGCACCTACGTGGAGGCCCCAGTTCTCGGAGGCCCCTCCGCCGCGAGGGCTGGCC
GGCTCGTAGTACTCTGCGGCTCCAAGAGGGGGGAGAGCGTCTGCCAGCGCCTCAAAGCTCTAGGCGAGACTGTATGGGTT
GGAGAGCCCCCCGCCGCCACGGCTGCAAAGCTGGCCTTCAACACGCTGTTCCTCTCGGTGGTAGCAGGCTTCGCGGAGGC
TGCAGCCCTCGTAGAGGCCTATGGGATTAGCTTCGACGAGTTCCTCGGGAGGATCATCTCGAAGACCTGGCTTGGAAGCA
TAGCTGAGCGATACGGCGAGCGGTTCAGGCTAGGCCACCCAGCCAGCTTTGCAGCCCGCCTCGCCGGGAAGGATGCGAGG
TATGCTGCTGAGGCTCTACAGGATGCCGGGCTCCCCGGACACCTGGCGGCAGCCGTTGCAGCATACTATGCGCTCATGGC
CGGGGAGGGCTACGGGGAGGAGGACTACCCAGCAATAGTAGGGTTCATGGCCAGCTACGCGAAAAAGAGGAGGCAGAAAG
GTAGAGGAGCCGTTTAG

Upstream 100 bases:

>100_bases
TACGGCTTGTGGAAATATGTGGTATAGCTTGAATATCTAGCTAAGGGGAATAAGAGTATGGGGCACCCAGGGGCCTAGAA
GGTGGGGAGGCCGGGACGCC

Downstream 100 bases:

>100_bases
GGGTACGGGTAGACTGTGAGACTGTAGAACTGCCAGGCCACCAGGCCCAGCAGTACCAGCAGCAGCGCCGCTGCAGTCGC
TGCAATGTCCGTGTTTCCAC

Product: 3-hydroxyisobutyrate dehydrogenase

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MEQCAAVLGLGRMGSAIAERLHDRGFRLVVWNRTRSRAEELAARLDAEVAGSPAGAAEKCNAVHIVVSDDEASMSVIAGP
TGITSINLGGRLVYNHTTVTPRHTMLALELVERAGGTYVEAPVLGGPSAARAGRLVVLCGSKRGESVCQRLKALGETVWV
GEPPAATAAKLAFNTLFLSVVAGFAEAAALVEAYGISFDEFLGRIISKTWLGSIAERYGERFRLGHPASFAARLAGKDAR
YAAEALQDAGLPGHLAAAVAAYYALMAGEGYGEEDYPAIVGFMASYAKKRRQKGRGAV

Sequences:

>Translated_298_residues
MEQCAAVLGLGRMGSAIAERLHDRGFRLVVWNRTRSRAEELAARLDAEVAGSPAGAAEKCNAVHIVVSDDEASMSVIAGP
TGITSINLGGRLVYNHTTVTPRHTMLALELVERAGGTYVEAPVLGGPSAARAGRLVVLCGSKRGESVCQRLKALGETVWV
GEPPAATAAKLAFNTLFLSVVAGFAEAAALVEAYGISFDEFLGRIISKTWLGSIAERYGERFRLGHPASFAARLAGKDAR
YAAEALQDAGLPGHLAAAVAAYYALMAGEGYGEEDYPAIVGFMASYAKKRRQKGRGAV
>Mature_298_residues
MEQCAAVLGLGRMGSAIAERLHDRGFRLVVWNRTRSRAEELAARLDAEVAGSPAGAAEKCNAVHIVVSDDEASMSVIAGP
TGITSINLGGRLVYNHTTVTPRHTMLALELVERAGGTYVEAPVLGGPSAARAGRLVVLCGSKRGESVCQRLKALGETVWV
GEPPAATAAKLAFNTLFLSVVAGFAEAAALVEAYGISFDEFLGRIISKTWLGSIAERYGERFRLGHPASFAARLAGKDAR
YAAEALQDAGLPGHLAAAVAAYYALMAGEGYGEEDYPAIVGFMASYAKKRRQKGRGAV

Specific function: Unknown

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI40556376, Length=279, Percent_Identity=30.4659498207885, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI23308751, Length=290, Percent_Identity=25.5172413793103, Blast_Score=82, Evalue=5e-16,
Organism=Escherichia coli, GI1790315, Length=282, Percent_Identity=27.3049645390071, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI145693186, Length=290, Percent_Identity=26.551724137931, Blast_Score=66, Evalue=3e-12,
Organism=Drosophila melanogaster, GI28574115, Length=290, Percent_Identity=27.2413793103448, Blast_Score=86, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 31337; Mature: 31337

Theoretical pI: Translated: 8.59; Mature: 8.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQCAAVLGLGRMGSAIAERLHDRGFRLVVWNRTRSRAEELAARLDAEVAGSPAGAAEKC
CCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCHHCC
NAVHIVVSDDEASMSVIAGPTGITSINLGGRLVYNHTTVTPRHTMLALELVERAGGTYVE
CEEEEEEECCCCCEEEEECCCCCEEECCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEE
APVLGGPSAARAGRLVVLCGSKRGESVCQRLKALGETVWVGEPPAATAAKLAFNTLFLSV
CCCCCCCCHHCCCCEEEEECCCCCHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHH
VAGFAEAAALVEAYGISFDEFLGRIISKTWLGSIAERYGERFRLGHPASFAARLAGKDAR
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHH
YAAEALQDAGLPGHLAAAVAAYYALMAGEGYGEEDYPAIVGFMASYAKKRRQKGRGAV
HHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MEQCAAVLGLGRMGSAIAERLHDRGFRLVVWNRTRSRAEELAARLDAEVAGSPAGAAEKC
CCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCHHCC
NAVHIVVSDDEASMSVIAGPTGITSINLGGRLVYNHTTVTPRHTMLALELVERAGGTYVE
CEEEEEEECCCCCEEEEECCCCCEEECCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEE
APVLGGPSAARAGRLVVLCGSKRGESVCQRLKALGETVWVGEPPAATAAKLAFNTLFLSV
CCCCCCCCHHCCCCEEEEECCCCCHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHH
VAGFAEAAALVEAYGISFDEFLGRIISKTWLGSIAERYGERFRLGHPASFAARLAGKDAR
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHH
YAAEALQDAGLPGHLAAAVAAYYALMAGEGYGEEDYPAIVGFMASYAKKRRQKGRGAV
HHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]