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The map label for this gene is nuoB [H]

Identifier: 124027080

GI number: 124027080

Start: 164231

End: 164860

Strand: Direct

Name: nuoB [H]

Synonym: Hbut_0183

Alternate gene names: 124027080

Gene position: 164231-164860 (Clockwise)

Preceding gene: 124027079

Following gene: 124027082

Centisome position: 9.85

GC content: 55.08

Gene sequence:

>630_bases
GTGGAGATGGCGTGTCCGCGTAAGGGTTTCATGCTCGTTGGCCGCTTGGAGGAGGCTGCCGCGAAGGCTATGAAGTGGTT
GCTCGAGAGGAAGCCTATCAAGTCCCTGCTGGACTGGGGTATAAGCTTCAGCCTCTGGCCCGTACACCTCACCACTAGCT
GCTGCGGCGCAGAGTTCGCAGCAGTCTACGACCCGAAGTATGACAGCGAGAGGCTTGGAAGTCTACCATTCACACATCCC
AGGAACACCAACGTACTGCTCGTGGAGGGCACCCTGACAAGAAAAATGGCCAGAGCCGCAAGGCTGGTCTGGGAGCAAAT
GCCATGGCCAAAGTTCGTCATAGCCATGGGGGCATGCGCTATAACAGGCGGCATATTCTACAATAGCTACAATATTGTGA
GGGTCAACCAAATCCTCCCCGTAGACTACTATGTGCCTGGCTGTCCTCCAACGCCAGAAGCTGTTGCTAACGCGCTGATA
AAGCTGCAGGAGAAAGTGAGGAAGGGCGAGTGGAAGCCAAGAGACATTGAGGATAAGAAGTTCCTAGAGGAGCTCATGGC
TCCACACTACGAGAAGGCAAGGAGGCACCTTGAACTGCTAGCCAGGACCCAGCGCACGGAAAGAGCATAA

Upstream 100 bases:

>100_bases
CCCTAAGCCTTGCGATTATAGCTTCTATCATTCCTCCACTCCGGTACGCTGTCCGCTATGCTGAGAAGAGGGAGTACTGG
GAGCTGGAGGTGTAGCCTCC

Downstream 100 bases:

>100_bases
ACCGGTAGGCATCACAATCAATCGCCGGGGCTCCAGAGAACGTCAGGGAAGCCTCCACAGCGTTTGTTAAAGGGACACCT
GCATCTTACATGGTTCCCCA

Product: NAD(P)H-quinone oxidoreductase chain K

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B [H]

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MEMACPRKGFMLVGRLEEAAAKAMKWLLERKPIKSLLDWGISFSLWPVHLTTSCCGAEFAAVYDPKYDSERLGSLPFTHP
RNTNVLLVEGTLTRKMARAARLVWEQMPWPKFVIAMGACAITGGIFYNSYNIVRVNQILPVDYYVPGCPPTPEAVANALI
KLQEKVRKGEWKPRDIEDKKFLEELMAPHYEKARRHLELLARTQRTERA

Sequences:

>Translated_209_residues
MEMACPRKGFMLVGRLEEAAAKAMKWLLERKPIKSLLDWGISFSLWPVHLTTSCCGAEFAAVYDPKYDSERLGSLPFTHP
RNTNVLLVEGTLTRKMARAARLVWEQMPWPKFVIAMGACAITGGIFYNSYNIVRVNQILPVDYYVPGCPPTPEAVANALI
KLQEKVRKGEWKPRDIEDKKFLEELMAPHYEKARRHLELLARTQRTERA
>Mature_209_residues
MEMACPRKGFMLVGRLEEAAAKAMKWLLERKPIKSLLDWGISFSLWPVHLTTSCCGAEFAAVYDPKYDSERLGSLPFTHP
RNTNVLLVEGTLTRKMARAARLVWEQMPWPKFVIAMGACAITGGIFYNSYNIVRVNQILPVDYYVPGCPPTPEAVANALI
KLQEKVRKGEWKPRDIEDKKFLEELMAPHYEKARRHLELLARTQRTERA

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=137, Percent_Identity=43.0656934306569, Blast_Score=136, Evalue=1e-32,
Organism=Escherichia coli, GI1788624, Length=138, Percent_Identity=39.8550724637681, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1789074, Length=151, Percent_Identity=37.0860927152318, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1788834, Length=125, Percent_Identity=36, Blast_Score=89, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17509685, Length=137, Percent_Identity=38.6861313868613, Blast_Score=121, Evalue=3e-28,
Organism=Drosophila melanogaster, GI18859983, Length=164, Percent_Identity=43.2926829268293, Blast_Score=146, Evalue=1e-35,
Organism=Drosophila melanogaster, GI24642371, Length=164, Percent_Identity=43.2926829268293, Blast_Score=146, Evalue=1e-35,
Organism=Drosophila melanogaster, GI24651058, Length=164, Percent_Identity=42.6829268292683, Blast_Score=141, Evalue=3e-34,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 23920; Mature: 23920

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEMACPRKGFMLVGRLEEAAAKAMKWLLERKPIKSLLDWGISFSLWPVHLTTSCCGAEFA
CCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCEEEHEEHHHHHHCCHHH
AVYDPKYDSERLGSLPFTHPRNTNVLLVEGTLTRKMARAARLVWEQMPWPKFVIAMGACA
HHCCCCCCHHHHCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
ITGGIFYNSYNIVRVNQILPVDYYVPGCPPTPEAVANALIKLQEKVRKGEWKPRDIEDKK
HHCCCEECCEEEEEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
FLEELMAPHYEKARRHLELLARTQRTERA
HHHHHHCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEMACPRKGFMLVGRLEEAAAKAMKWLLERKPIKSLLDWGISFSLWPVHLTTSCCGAEFA
CCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCEEEHEEHHHHHHCCHHH
AVYDPKYDSERLGSLPFTHPRNTNVLLVEGTLTRKMARAARLVWEQMPWPKFVIAMGACA
HHCCCCCCHHHHCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
ITGGIFYNSYNIVRVNQILPVDYYVPGCPPTPEAVANALIKLQEKVRKGEWKPRDIEDKK
HHCCCEECCEEEEEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
FLEELMAPHYEKARRHLELLARTQRTERA
HHHHHHCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA