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The map label for this gene is gcvH [H]

Identifier: 124027078

GI number: 124027078

Start: 163244

End: 163663

Strand: Reverse

Name: gcvH [H]

Synonym: Hbut_0181

Alternate gene names: 124027078

Gene position: 163663-163244 (Counterclockwise)

Preceding gene: 124027081

Following gene: 124027074

Centisome position: 9.82

GC content: 59.29

Gene sequence:

>420_bases
TTGGCTGGAGAGATACGTGTCGGAGACTACCTCGTCCTAACCGATAGGAGGTACACAAAGTCCGACGAGTGGATCAAGGA
GGAGGACGGTGTCGTCACCGTTGGGATAACGGATTACGCGCAGAAGAAGCTACGGGACATCGTTGGCGTAGACCTGCCCG
AGAAGGGTAGGAGGGTGCAGGCGGGGGAGGCCGTGGCGAGCATAGAGTCGGTTAAGGCCGCCGCAGACGTCTATGCGCCG
GTCAGCGGGGAGGTGGTGGAGGTTAACGAGAGGCTCTACGATGAGCCGGAGCTGCTGAACAGGGACCCCTATGGGGAGGG
CTGGATGTTCAAGATAAAGATTGAGGATCGCTCCGAGCTGGAGAAGCTGCTTAGCCCCGAGGAGTACGCCGAGAAGATCA
GGAAGGAGGAGGGTCTCTAG

Upstream 100 bases:

>100_bases
CTAAACCGTAATGCACCATCCACAGTTCTGTTAGCCTTAAGAGCCCTTAGCCTTAGCATTCTGTAGACCTGGTGCTTGCT
GCTCCCTGGGTGCATAAGCA

Downstream 100 bases:

>100_bases
CCCTCCCTCTTCTTCAGCCCAAGTCTCATCTGCGTTTTTGGCTTAACCCTACCAGTCTTCTTCCAGTCACCCTTATCGTG
ACGTTACGGCCACGCCTTTT

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 139; Mature: 138

Protein sequence:

>139_residues
MAGEIRVGDYLVLTDRRYTKSDEWIKEEDGVVTVGITDYAQKKLRDIVGVDLPEKGRRVQAGEAVASIESVKAAADVYAP
VSGEVVEVNERLYDEPELLNRDPYGEGWMFKIKIEDRSELEKLLSPEEYAEKIRKEEGL

Sequences:

>Translated_139_residues
MAGEIRVGDYLVLTDRRYTKSDEWIKEEDGVVTVGITDYAQKKLRDIVGVDLPEKGRRVQAGEAVASIESVKAAADVYAP
VSGEVVEVNERLYDEPELLNRDPYGEGWMFKIKIEDRSELEKLLSPEEYAEKIRKEEGL
>Mature_138_residues
AGEIRVGDYLVLTDRRYTKSDEWIKEEDGVVTVGITDYAQKKLRDIVGVDLPEKGRRVQAGEAVASIESVKAAADVYAPV
SGEVVEVNERLYDEPELLNRDPYGEGWMFKIKIEDRSELEKLLSPEEYAEKIRKEEGL

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI49574537, Length=133, Percent_Identity=41.3533834586466, Blast_Score=117, Evalue=3e-27,
Organism=Homo sapiens, GI89057342, Length=133, Percent_Identity=40.6015037593985, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1789271, Length=119, Percent_Identity=57.1428571428571, Blast_Score=134, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17507493, Length=133, Percent_Identity=45.1127819548872, Blast_Score=128, Evalue=9e-31,
Organism=Caenorhabditis elegans, GI17551294, Length=122, Percent_Identity=45.0819672131148, Blast_Score=119, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6319272, Length=121, Percent_Identity=41.3223140495868, Blast_Score=98, Evalue=5e-22,
Organism=Drosophila melanogaster, GI17865652, Length=120, Percent_Identity=41.6666666666667, Blast_Score=104, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 15764; Mature: 15633

Theoretical pI: Translated: 4.33; Mature: 4.33

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGEIRVGDYLVLTDRRYTKSDEWIKEEDGVVTVGITDYAQKKLRDIVGVDLPEKGRRVQ
CCCCEECCCEEEEECCCCCCCCCCEECCCCEEEEECHHHHHHHHHHHHCCCCCCCCCEEC
AGEAVASIESVKAAADVYAPVSGEVVEVNERLYDEPELLNRDPYGEGWMFKIKIEDRSEL
CHHHHHHHHHHHHHHHHCCCCCCCEEEECHHHCCCHHHCCCCCCCCCCEEEEEECCHHHH
EKLLSPEEYAEKIRKEEGL
HHHCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
AGEIRVGDYLVLTDRRYTKSDEWIKEEDGVVTVGITDYAQKKLRDIVGVDLPEKGRRVQ
CCCEECCCEEEEECCCCCCCCCCEECCCCEEEEECHHHHHHHHHHHHCCCCCCCCCEEC
AGEAVASIESVKAAADVYAPVSGEVVEVNERLYDEPELLNRDPYGEGWMFKIKIEDRSEL
CHHHHHHHHHHHHHHHHCCCCCCCEEEECHHHCCCHHHCCCCCCCCCCEEEEEECCHHHH
EKLLSPEEYAEKIRKEEGL
HHHCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]