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The map label for this gene is ytsJ [H]

Identifier: 124027050

GI number: 124027050

Start: 137603

End: 139000

Strand: Reverse

Name: ytsJ [H]

Synonym: Hbut_0153

Alternate gene names: 124027050

Gene position: 139000-137603 (Counterclockwise)

Preceding gene: 124027055

Following gene: 124027041

Centisome position: 8.34

GC content: 55.44

Gene sequence:

>1398_bases
ATGTCCGAAAAAGAGAGGATAGACTGGTATAGTATCTCCAAGAAACTACACCGGCTCTACGAGGGCAAGATAGAGGTTAT
ACCTAAGGTTCCTGCATTTCGCCAAGACGACTTCGCTATCTGGTACACCCCTGGCGTAGCAGGCCCCGCCAAGGAGGACG
CCGAGGATCCAGACGCAACATTCCACAATACCTGGCGTTGGAACACCGTCGCCGTTGTAAGCGACGGTACTAGGGTTCTA
GGCCTAGGCGCTATTGGGCCCGAAGGCGCCCTCCCGGTAATGGAGGGTAAGGCCCTTCTCTTCAAAGCCCTGGGCGGCGT
TGATGCCGTGCCCCTAGTTCACAGGGCGAGGGATCGGGAGAAGTTCCTCGAGCTACTCGTGTTAATAGAGCCGAGCTTTG
GCGGCATAAACCTCGAGGATATCGAGAGCCCCAAGTGCTTCTACCTCCTTGACGAGGCTCGGAAACGGCTAAACATACCC
ATATGGCATGACGACCAGCAGGGTACCGCAACCGTAACCCTTGCAGGCCTCATAAACGCCGCTAAGCTCACTGGCAGAGA
CCTGCGCAAGATGAGGATAGCACTCATAGGTGCCGGTGCCGCCAACACTGCACTTTACAGACTGTTGAAGGCCTATGGCG
TAGATCCCAAGAATATGGTCGTGGTAGACTCTAAGGGTGTACTGCACAGCCAGAGAGAGGACATCGAGAAGCTGAAGGAG
AAGAACCCGTGGAAGTACCAGATAGCAGTCGAGACCGACGGCGGCTGGAACCAGCTACGAGGCGGCGGCATAAAGGAGGC
CCTCGAAGGTGCAGAAGCTGTTGTAGCGGCTTCCAAGCCAGGGCCAGGCGTGATAAAGCCCGAGTGGATAAAGCTAATGG
ACAAGGACCCCATAGTCTTCGCCGAGGCTAACCCTGTGCCAGAGATATGGCCATGGGAGGCTAAGGAGGCTGGTGCAAGG
GTCGTAGCGACTGGGCGCAGCGACTTCCCCAACCAGGTAAACAATAGCCTTGCATTCCCCTCAATATTCCGTGGCGTACT
GGACGTACGCGCCAGGACAATCACCGACACCATGGCTATAGCTGCTGCCGAGGAGCTGGCAAGATTCGCCGAGGAGAAGG
GGCTACGAGAAGACTACATCATACCATCGATGGAAGAGTGGGAGGTATATCCACGCGTAGCCGCCGCAACCGCAGTAAGG
GCTGTGGAGGAGGGAGTAGCAAGGCTCAAGACTACATACGAGGAGGAGTACGAGAAGGCCAAACAGATAATCGCTACGAG
CAGAGAGAAGGTACTCAAACTAGTAGACGTAGGCCTCATAAAGAAGCTGCCCGAAGAGGTAGAAGCTGTAATACAGCGTG
CTAGACGCCAGAGGGAAGAAATGCTCAAAGCTAGATAG

Upstream 100 bases:

>100_bases
AAACCTGTCACTGAACATTTTCAATCATTCTAAACCACAACATGTTTTAACATTTACAAATCGTTACCAAACACGGCCAG
CTAGAGGTGTCCAAAATATC

Downstream 100 bases:

>100_bases
ACCCAAAAAGACTAATCAATTCCGTTTTTTACGGAGACGCTGGCTCCTCTCCATGGACACTCCGGCTGTACTGCTAGAGG
AGCCGCCCGAGGAGCCTCTC

Product: malic enzyme

Products: NA

Alternate protein names: NAD-ME 4 [H]

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MSEKERIDWYSISKKLHRLYEGKIEVIPKVPAFRQDDFAIWYTPGVAGPAKEDAEDPDATFHNTWRWNTVAVVSDGTRVL
GLGAIGPEGALPVMEGKALLFKALGGVDAVPLVHRARDREKFLELLVLIEPSFGGINLEDIESPKCFYLLDEARKRLNIP
IWHDDQQGTATVTLAGLINAAKLTGRDLRKMRIALIGAGAANTALYRLLKAYGVDPKNMVVVDSKGVLHSQREDIEKLKE
KNPWKYQIAVETDGGWNQLRGGGIKEALEGAEAVVAASKPGPGVIKPEWIKLMDKDPIVFAEANPVPEIWPWEAKEAGAR
VVATGRSDFPNQVNNSLAFPSIFRGVLDVRARTITDTMAIAAAEELARFAEEKGLREDYIIPSMEEWEVYPRVAAATAVR
AVEEGVARLKTTYEEEYEKAKQIIATSREKVLKLVDVGLIKKLPEEVEAVIQRARRQREEMLKAR

Sequences:

>Translated_465_residues
MSEKERIDWYSISKKLHRLYEGKIEVIPKVPAFRQDDFAIWYTPGVAGPAKEDAEDPDATFHNTWRWNTVAVVSDGTRVL
GLGAIGPEGALPVMEGKALLFKALGGVDAVPLVHRARDREKFLELLVLIEPSFGGINLEDIESPKCFYLLDEARKRLNIP
IWHDDQQGTATVTLAGLINAAKLTGRDLRKMRIALIGAGAANTALYRLLKAYGVDPKNMVVVDSKGVLHSQREDIEKLKE
KNPWKYQIAVETDGGWNQLRGGGIKEALEGAEAVVAASKPGPGVIKPEWIKLMDKDPIVFAEANPVPEIWPWEAKEAGAR
VVATGRSDFPNQVNNSLAFPSIFRGVLDVRARTITDTMAIAAAEELARFAEEKGLREDYIIPSMEEWEVYPRVAAATAVR
AVEEGVARLKTTYEEEYEKAKQIIATSREKVLKLVDVGLIKKLPEEVEAVIQRARRQREEMLKAR
>Mature_464_residues
SEKERIDWYSISKKLHRLYEGKIEVIPKVPAFRQDDFAIWYTPGVAGPAKEDAEDPDATFHNTWRWNTVAVVSDGTRVLG
LGAIGPEGALPVMEGKALLFKALGGVDAVPLVHRARDREKFLELLVLIEPSFGGINLEDIESPKCFYLLDEARKRLNIPI
WHDDQQGTATVTLAGLINAAKLTGRDLRKMRIALIGAGAANTALYRLLKAYGVDPKNMVVVDSKGVLHSQREDIEKLKEK
NPWKYQIAVETDGGWNQLRGGGIKEALEGAEAVVAASKPGPGVIKPEWIKLMDKDPIVFAEANPVPEIWPWEAKEAGARV
VATGRSDFPNQVNNSLAFPSIFRGVLDVRARTITDTMAIAAAEELARFAEEKGLREDYIIPSMEEWEVYPRVAAATAVRA
VEEGVARLKTTYEEEYEKAKQIIATSREKVLKLVDVGLIKKLPEEVEAVIQRARRQREEMLKAR

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malic enzymes family [H]

Homologues:

Organism=Homo sapiens, GI4505143, Length=447, Percent_Identity=25.9507829977629, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI1788806, Length=401, Percent_Identity=41.645885286783, Blast_Score=294, Evalue=7e-81,
Organism=Saccharomyces cerevisiae, GI6322823, Length=397, Percent_Identity=26.448362720403, Blast_Score=97, Evalue=5e-21,
Organism=Drosophila melanogaster, GI78707242, Length=399, Percent_Identity=26.5664160401003, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI78707238, Length=399, Percent_Identity=26.5664160401003, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI78707240, Length=396, Percent_Identity=26.5151515151515, Blast_Score=93, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR001891
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M [H]

EC number: =1.1.1.38 [H]

Molecular weight: Translated: 51736; Mature: 51605

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKERIDWYSISKKLHRLYEGKIEVIPKVPAFRQDDFAIWYTPGVAGPAKEDAEDPDAT
CCCCHHCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCCCCC
FHNTWRWNTVAVVSDGTRVLGLGAIGPEGALPVMEGKALLFKALGGVDAVPLVHRARDRE
CCCCCCEEEEEEEECCCEEEEEECCCCCCCCCHHCCHHHHHHHHCCCCCHHHHHHHHHHH
KFLELLVLIEPSFGGINLEDIESPKCFYLLDEARKRLNIPIWHDDQQGTATVTLAGLINA
HHHHHHHEECCCCCCCCHHHCCCCCEEEEEHHHHHHCCCCEEECCCCCCEEEEHHHHHHH
AKLTGRDLRKMRIALIGAGAANTALYRLLKAYGVDPKNMVVVDSKGVLHSQREDIEKLKE
HHHHHHHHHHEEEEEEECCHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHH
KNPWKYQIAVETDGGWNQLRGGGIKEALEGAEAVVAASKPGPGVIKPEWIKLMDKDPIVF
CCCCEEEEEEECCCCHHHHCCCCHHHHHCCCCEEEEECCCCCCCCCHHHHHEECCCCEEE
AEANPVPEIWPWEAKEAGARVVATGRSDFPNQVNNSLAFPSIFRGVLDVRARTITDTMAI
ECCCCCCCCCCCCCHHCCCEEEEECCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
AAAEELARFAEEKGLREDYIIPSMEEWEVYPRVAAATAVRAVEEGVARLKTTYEEEYEKA
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KQIIATSREKVLKLVDVGLIKKLPEEVEAVIQRARRQREEMLKAR
HHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SEKERIDWYSISKKLHRLYEGKIEVIPKVPAFRQDDFAIWYTPGVAGPAKEDAEDPDAT
CCCHHCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCCCCC
FHNTWRWNTVAVVSDGTRVLGLGAIGPEGALPVMEGKALLFKALGGVDAVPLVHRARDRE
CCCCCCEEEEEEEECCCEEEEEECCCCCCCCCHHCCHHHHHHHHCCCCCHHHHHHHHHHH
KFLELLVLIEPSFGGINLEDIESPKCFYLLDEARKRLNIPIWHDDQQGTATVTLAGLINA
HHHHHHHEECCCCCCCCHHHCCCCCEEEEEHHHHHHCCCCEEECCCCCCEEEEHHHHHHH
AKLTGRDLRKMRIALIGAGAANTALYRLLKAYGVDPKNMVVVDSKGVLHSQREDIEKLKE
HHHHHHHHHHEEEEEEECCHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHH
KNPWKYQIAVETDGGWNQLRGGGIKEALEGAEAVVAASKPGPGVIKPEWIKLMDKDPIVF
CCCCEEEEEEECCCCHHHHCCCCHHHHHCCCCEEEEECCCCCCCCCHHHHHEECCCCEEE
AEANPVPEIWPWEAKEAGARVVATGRSDFPNQVNNSLAFPSIFRGVLDVRARTITDTMAI
ECCCCCCCCCCCCCHHCCCEEEEECCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
AAAEELARFAEEKGLREDYIIPSMEEWEVYPRVAAATAVRAVEEGVARLKTTYEEEYEKA
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KQIIATSREKVLKLVDVGLIKKLPEEVEAVIQRARRQREEMLKAR
HHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]