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The map label for this gene is endA

Identifier: 124026948

GI number: 124026948

Start: 35980

End: 36525

Strand: Reverse

Name: endA

Synonym: Hbut_0045

Alternate gene names: 124026948

Gene position: 36525-35980 (Counterclockwise)

Preceding gene: 124026949

Following gene: 124026947

Centisome position: 2.19

GC content: 51.83

Gene sequence:

>546_bases
ATGATACGGGCTGTATTGCTGGGGCTCAGAGCTGTAGTGTTTGACCGGGAGGCAGCCAGAAGGCTCTATGCAAGCGGATA
CTATGGTAAGCCTTTAGGCATACCTAAGCCCCGTGGCTCTAATTTTGACGCGCCGCTAGAGCTTGGGCTCCTGGAGACAC
TCCACCTGCTAGAGAGGGGTATGCTAGAGGTCTACGACGAGGAAGGCCACAGGGTAAGTGTTGAGGAGGTAAGGAGGCGA
GCTGAAAGGTTTATACCTAAATTTGAGCTGCTCTACAAGGTTTATCGTGACTTGAGAGACCGGGGCTATGTTGTTAGGTC
TGGGCTCAAATATGGCTCAGACTTTGCCGTGTATGAACGCGGACCTGGGCTCGAGCATGCACCCTATCTAGTCCATGTTA
TGCGTGTGGACGAGGAGATAGATCCTCTTGAAATAGTTCGCGCGGGAAGGCTTAGCCATAGTGTTAGGAAGGCATTTATC
CTAGCGCTTACAGGTCCGAGGGATACACCGATACGCTACCTGTTGCTTAAGTGGAGTAAGCCCTAA

Upstream 100 bases:

>100_bases
ATCTCATCGAAGATATAGCTGATACTATTAGGGATGCTGCTGAAAACGTTAAGTTCCTAGCACTCTACATGGTTGCCGGC
CGCTAGACAACGGTGATGCA

Downstream 100 bases:

>100_bases
TAAGATTGGCCAGAGAGACTGTAAGTGGCAAGCCAGCATTGCCTACGCTCTCTCGCCGGATGGTGCATAGCAGTATGCTG
GGCGAGGTGTAGAAATTTGT

Product: tRNA-splicing endonuclease subunit alpha

Products: NA

Alternate protein names: tRNA-intron endonuclease

Number of amino acids: Translated: 181; Mature: 181

Protein sequence:

>181_residues
MIRAVLLGLRAVVFDREAARRLYASGYYGKPLGIPKPRGSNFDAPLELGLLETLHLLERGMLEVYDEEGHRVSVEEVRRR
AERFIPKFELLYKVYRDLRDRGYVVRSGLKYGSDFAVYERGPGLEHAPYLVHVMRVDEEIDPLEIVRAGRLSHSVRKAFI
LALTGPRDTPIRYLLLKWSKP

Sequences:

>Translated_181_residues
MIRAVLLGLRAVVFDREAARRLYASGYYGKPLGIPKPRGSNFDAPLELGLLETLHLLERGMLEVYDEEGHRVSVEEVRRR
AERFIPKFELLYKVYRDLRDRGYVVRSGLKYGSDFAVYERGPGLEHAPYLVHVMRVDEEIDPLEIVRAGRLSHSVRKAFI
LALTGPRDTPIRYLLLKWSKP
>Mature_181_residues
MIRAVLLGLRAVVFDREAARRLYASGYYGKPLGIPKPRGSNFDAPLELGLLETLHLLERGMLEVYDEEGHRVSVEEVRRR
AERFIPKFELLYKVYRDLRDRGYVVRSGLKYGSDFAVYERGPGLEHAPYLVHVMRVDEEIDPLEIVRAGRLSHSVRKAFI
LALTGPRDTPIRYLLLKWSKP

Specific function: Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate

COG id: COG1676

COG function: function code J; tRNA splicing endonuclease

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA-intron endonuclease family. Archaeal short subfamily

Homologues:

Organism=Homo sapiens, GI116875840, Length=95, Percent_Identity=37.8947368421053, Blast_Score=66, Evalue=2e-11,
Organism=Homo sapiens, GI116875842, Length=95, Percent_Identity=37.8947368421053, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ENDA_HYPBU (A2BIW2)

Other databases:

- EMBL:   CP000493
- RefSeq:   YP_001012268.1
- ProteinModelPortal:   A2BIW2
- SMR:   A2BIW2
- STRING:   A2BIW2
- GeneID:   4782745
- GenomeReviews:   CP000493_GR
- KEGG:   hbu:Hbut_0045
- eggNOG:   arNOG05458
- HOGENOM:   HBG297289
- OMA:   DFRLYER
- ProtClustDB:   PRK09297
- BioCyc:   HBUT415426:HBUT_0045-MONOMER
- HAMAP:   MF_01833
- InterPro:   IPR011856
- InterPro:   IPR006676
- InterPro:   IPR006677
- InterPro:   IPR006678
- InterPro:   IPR016442
- Gene3D:   G3DSA:3.40.1350.10
- PIRSF:   PIRSF005285
- TIGRFAMs:   TIGR00324

Pfam domain/function: PF01974 tRNA_int_endo; PF02778 tRNA_int_endo_N; SSF53032 tRNA_int_endo_C; SSF55267 tRNA_int_endo_N

EC number: =3.1.27.9

Molecular weight: Translated: 20893; Mature: 20893

Theoretical pI: Translated: 9.88; Mature: 9.88

Prosite motif: NA

Important sites: ACT_SITE 118-118 ACT_SITE 126-126 ACT_SITE 157-157

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRAVLLGLRAVVFDREAARRLYASGYYGKPLGIPKPRGSNFDAPLELGLLETLHLLERG
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
MLEVYDEEGHRVSVEEVRRRAERFIPKFELLYKVYRDLRDRGYVVRSGLKYGSDFAVYER
HHHHHCCCCCEECHHHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEECCCCCCCCEEEEEC
GPGLEHAPYLVHVMRVDEEIDPLEIVRAGRLSHSVRKAFILALTGPRDTPIRYLLLKWSK
CCCCCCCCHHEEEEHHCCCCCHHHHHHHHHHHHHHHHEEEEEEECCCCCCEEEEEEEECC
P
C
>Mature Secondary Structure
MIRAVLLGLRAVVFDREAARRLYASGYYGKPLGIPKPRGSNFDAPLELGLLETLHLLERG
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
MLEVYDEEGHRVSVEEVRRRAERFIPKFELLYKVYRDLRDRGYVVRSGLKYGSDFAVYER
HHHHHCCCCCEECHHHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEECCCCCCCCEEEEEC
GPGLEHAPYLVHVMRVDEEIDPLEIVRAGRLSHSVRKAFILALTGPRDTPIRYLLLKWSK
CCCCCCCCHHEEEEHHCCCCCHHHHHHHHHHHHHHHHEEEEEEECCCCCCEEEEEEEECC
P
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA