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The map label for this gene is pcnA [H]

Identifier: 124026921

GI number: 124026921

Start: 14098

End: 14844

Strand: Direct

Name: pcnA [H]

Synonym: Hbut_0018

Alternate gene names: 124026921

Gene position: 14098-14844 (Clockwise)

Preceding gene: 124026918

Following gene: 124026928

Centisome position: 0.85

GC content: 47.52

Gene sequence:

>747_bases
ATGGCTAAGGCAAGACTGTCATACCCTGACGCAAAATCGTTTTACAACATGCTTGATGCGATATCGAAAATTGTTGATGA
GCTTGCAATGACTATAACGCCTAATGGCGTAAAAGCGATAGCCCTTGACCCAGCCCATGTAGCGCTAATGGTCATGGAGC
TGCCGCCAGAAAGCTTCATAGAGTACGAGGTTGAGGATGAAGTGAAGCTTGGTTTTAATGTTGCTAACATAGCGAAGATA
ATTAAGAGGGGTAAGAAGGGCGATAAGCTAGACATAGAAGTCGACGAGGATAGGGTTACATGGTCTATTGTTGGAGCTAC
GATCAAAAGGTATAGACTGCTAAATCTTGACGTGCCGGTGCCAGAGCTACCAGAGGCAGAGCTTGAGTTTAAGGTACACG
TTTCACTGATAGTGGATCCGTTCAAGAACGCACTAAAAGATGCTGAAGCCGTCGGGGATACTGTTGAGCTAGAGGCACCA
GACGACACGACACTAATCATACGTGGTGTCGGAGCTGCAGTAGCGGAGACAAAGATTAGGAGTGATACGCCTGCCGTAGT
GGAGTTTAATGTTGAGGAGCCCTCCAAGTCAGCCTATAGTATAGAGTACTTGAAGCATATGCTTTCGCTGACAAAGGTTG
CAGACACGATAACCATAGAATTTGCGCAGGACATGCCGCTAAGGCTACAGTTCAGGTTACCTGCTGGCGGCAACGTGACA
TATCTCCTAGCGCCGAAAGCGGCATAG

Upstream 100 bases:

>100_bases
GCGTTTTGCCGTGGTGGATTATTATCTCGTTATGCAAGTGAGAGAAACCCTCACTCTTATACCCTCACAGAAACCCATAT
CCTGGGTTAGGGTGCAGGGG

Downstream 100 bases:

>100_bases
CGCACCGGGCAGTGATGAGTTTCACCGGCTCATCCGACGCTCTCTAGGCCGTGAGGATGGCCAGGACAGCTGAGCCACCT
ACTTCTTCACAAGTTCGAGT

Product: DNA polymerase sliding clamp subunit

Products: NA

Alternate protein names: Proliferating cell nuclear antigen homolog A; PCNA A [H]

Number of amino acids: Translated: 248; Mature: 247

Protein sequence:

>248_residues
MAKARLSYPDAKSFYNMLDAISKIVDELAMTITPNGVKAIALDPAHVALMVMELPPESFIEYEVEDEVKLGFNVANIAKI
IKRGKKGDKLDIEVDEDRVTWSIVGATIKRYRLLNLDVPVPELPEAELEFKVHVSLIVDPFKNALKDAEAVGDTVELEAP
DDTTLIIRGVGAAVAETKIRSDTPAVVEFNVEEPSKSAYSIEYLKHMLSLTKVADTITIEFAQDMPLRLQFRLPAGGNVT
YLLAPKAA

Sequences:

>Translated_248_residues
MAKARLSYPDAKSFYNMLDAISKIVDELAMTITPNGVKAIALDPAHVALMVMELPPESFIEYEVEDEVKLGFNVANIAKI
IKRGKKGDKLDIEVDEDRVTWSIVGATIKRYRLLNLDVPVPELPEAELEFKVHVSLIVDPFKNALKDAEAVGDTVELEAP
DDTTLIIRGVGAAVAETKIRSDTPAVVEFNVEEPSKSAYSIEYLKHMLSLTKVADTITIEFAQDMPLRLQFRLPAGGNVT
YLLAPKAA
>Mature_247_residues
AKARLSYPDAKSFYNMLDAISKIVDELAMTITPNGVKAIALDPAHVALMVMELPPESFIEYEVEDEVKLGFNVANIAKII
KRGKKGDKLDIEVDEDRVTWSIVGATIKRYRLLNLDVPVPELPEAELEFKVHVSLIVDPFKNALKDAEAVGDTVELEAPD
DTTLIIRGVGAAVAETKIRSDTPAVVEFNVEEPSKSAYSIEYLKHMLSLTKVADTITIEFAQDMPLRLQFRLPAGGNVTY
LLAPKAA

Specific function: Sliding clamp subunit. Responsible for tethering the catalytic subunit of DNA polymerase to DNA during high-speed replication [H]

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PCNA family [H]

Homologues:

Organism=Homo sapiens, GI33239451, Length=242, Percent_Identity=23.1404958677686, Blast_Score=84, Evalue=8e-17,
Organism=Homo sapiens, GI4505641, Length=242, Percent_Identity=23.1404958677686, Blast_Score=84, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI71995072, Length=220, Percent_Identity=22.2727272727273, Blast_Score=65, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6319564, Length=249, Percent_Identity=21.6867469879518, Blast_Score=72, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24585303, Length=253, Percent_Identity=22.5296442687747, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI17136790, Length=258, Percent_Identity=22.4806201550388, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI45552759, Length=258, Percent_Identity=22.4806201550388, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000730
- InterPro:   IPR022649
- InterPro:   IPR022659
- InterPro:   IPR022648 [H]

Pfam domain/function: PF02747 PCNA_C; PF00705 PCNA_N [H]

EC number: NA

Molecular weight: Translated: 27311; Mature: 27180

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKARLSYPDAKSFYNMLDAISKIVDELAMTITPNGVKAIALDPAHVALMVMELPPESFI
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCEEEEEECCHHEEEEEEECCCHHHE
EYEVEDEVKLGFNVANIAKIIKRGKKGDKLDIEVDEDRVTWSIVGATIKRYRLLNLDVPV
EEECCCCEEECCCHHHHHHHHHCCCCCCEEEEEECCCEEEEEEEHHHHHEEEEEECCCCC
PELPEAELEFKVHVSLIVDPFKNALKDAEAVGDTVELEAPDDTTLIIRGVGAAVAETKIR
CCCCCCCEEEEEEEEEEEHHHHHHHHHHHHCCCEEEEECCCCCEEEEEECCCHHHHHCCC
SDTPAVVEFNVEEPSKSAYSIEYLKHMLSLTKVADTITIEFAQDMPLRLQFRLPAGGNVT
CCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHEEEEEECCCCCEEEEEECCCCCCEE
YLLAPKAA
EEEECCCC
>Mature Secondary Structure 
AKARLSYPDAKSFYNMLDAISKIVDELAMTITPNGVKAIALDPAHVALMVMELPPESFI
CCCCCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCEEEEEECCHHEEEEEEECCCHHHE
EYEVEDEVKLGFNVANIAKIIKRGKKGDKLDIEVDEDRVTWSIVGATIKRYRLLNLDVPV
EEECCCCEEECCCHHHHHHHHHCCCCCCEEEEEECCCEEEEEEEHHHHHEEEEEECCCCC
PELPEAELEFKVHVSLIVDPFKNALKDAEAVGDTVELEAPDDTTLIIRGVGAAVAETKIR
CCCCCCCEEEEEEEEEEEHHHHHHHHHHHHCCCEEEEECCCCCEEEEEECCCHHHHHCCC
SDTPAVVEFNVEEPSKSAYSIEYLKHMLSLTKVADTITIEFAQDMPLRLQFRLPAGGNVT
CCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHEEEEEECCCCCEEEEEECCCCCCEE
YLLAPKAA
EEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]