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The map label for this gene is purU [H]

Identifier: 123967126

GI number: 123967126

Start: 1685679

End: 1686533

Strand: Reverse

Name: purU [H]

Synonym: P9515_18931

Alternate gene names: 123967126

Gene position: 1686533-1685679 (Counterclockwise)

Preceding gene: 123967128

Following gene: 123967123

Centisome position: 98.96

GC content: 32.05

Gene sequence:

>855_bases
TTGGAAAGACCATCTATTATATTCAGAACAGTTTGTCCTGATCGTTCCGGACTAGTTAGTCAATTAACTAGTTGGATATC
TAATTATGGGGGCAATATCAAACATTCAGATCACCATACAGATCAAGAAGCAGGATTGTTTTTGAGTAGAATAGAATGGA
ATTTAAATGATTTACCAATTAATAAAGTTGAAATCTATGAGCAATTTGAGAAAAATGCAATAGATATAAATGGCCAATTT
AATATTAATTACTCAGATGAGATACCAAATGTAGGTATTTTTGTAAGTAAGCAAAATCACTGTTTAATAGACTTGCTTTG
GCGAGTAAGAAACGGCGAATTAAAAATGAATGTACCATTAATAATTTCTAATCATCCTGATCTTGAAGAAATCGCTAAGG
ATTTTAATGCCCAATTTGTTTATATTGATAATTTAAAATACTCTAAATCTACTGTCGAAAACCAGATTTTAAATTTGTTA
AATGATTTTGATATCGAACTGGTTGTATTAGCCAAATATATGCAAATTTTAAGTGATTCTTTTTTGAAAAGTTATTCCTC
AATTATTAACATACATCATTCTTTTTTGCCTGCATTTAAAGGCGCGCAACCTTATCATCGAGCATGGAAGAGAGGTGTGA
AATTGATTGGTGCGACTGCACATTATGTTACTCAAGATTTAGACGAAGGGCCAATAATTGAGCAATGTACTGTCAATGTA
AGTCATAGAGATGAAGTTGCTGATTTGATTAGAAAAGGACGCGATACTGAGAGGATTGCGCTTGCAAGGGCAGTTAGATT
ACATTTGAATCATCAGATTTTCGTATACGATAGCAAAACAGCTGTTTTTGATTAG

Upstream 100 bases:

>100_bases
CTGAACCGATAATTAATATATGAGATTTTTTAAAATGTTTTTTTAATACTTTCATTGATATATTATATGAAATTAACTAA
AAAATTAAATACTTAGATTT

Downstream 100 bases:

>100_bases
AAATCAATTTTATTCTTCTAATTCAATCTGTATTTGTCTTTCATAAATAGATTTTTCATTAAAAGCTCTTGCCACTAAAA
AACTTGTAATACAACTAATA

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MERPSIIFRTVCPDRSGLVSQLTSWISNYGGNIKHSDHHTDQEAGLFLSRIEWNLNDLPINKVEIYEQFEKNAIDINGQF
NINYSDEIPNVGIFVSKQNHCLIDLLWRVRNGELKMNVPLIISNHPDLEEIAKDFNAQFVYIDNLKYSKSTVENQILNLL
NDFDIELVVLAKYMQILSDSFLKSYSSIINIHHSFLPAFKGAQPYHRAWKRGVKLIGATAHYVTQDLDEGPIIEQCTVNV
SHRDEVADLIRKGRDTERIALARAVRLHLNHQIFVYDSKTAVFD

Sequences:

>Translated_284_residues
MERPSIIFRTVCPDRSGLVSQLTSWISNYGGNIKHSDHHTDQEAGLFLSRIEWNLNDLPINKVEIYEQFEKNAIDINGQF
NINYSDEIPNVGIFVSKQNHCLIDLLWRVRNGELKMNVPLIISNHPDLEEIAKDFNAQFVYIDNLKYSKSTVENQILNLL
NDFDIELVVLAKYMQILSDSFLKSYSSIINIHHSFLPAFKGAQPYHRAWKRGVKLIGATAHYVTQDLDEGPIIEQCTVNV
SHRDEVADLIRKGRDTERIALARAVRLHLNHQIFVYDSKTAVFD
>Mature_284_residues
MERPSIIFRTVCPDRSGLVSQLTSWISNYGGNIKHSDHHTDQEAGLFLSRIEWNLNDLPINKVEIYEQFEKNAIDINGQF
NINYSDEIPNVGIFVSKQNHCLIDLLWRVRNGELKMNVPLIISNHPDLEEIAKDFNAQFVYIDNLKYSKSTVENQILNLL
NDFDIELVVLAKYMQILSDSFLKSYSSIINIHHSFLPAFKGAQPYHRAWKRGVKLIGATAHYVTQDLDEGPIIEQCTVNV
SHRDEVADLIRKGRDTERIALARAVRLHLNHQIFVYDSKTAVFD

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=204, Percent_Identity=32.3529411764706, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI209869995, Length=204, Percent_Identity=32.3529411764706, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI209869993, Length=204, Percent_Identity=32.3529411764706, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1787483, Length=285, Percent_Identity=35.0877192982456, Blast_Score=184, Evalue=4e-48,
Organism=Escherichia coli, GI1788846, Length=172, Percent_Identity=27.3255813953488, Blast_Score=72, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17567511, Length=165, Percent_Identity=33.9393939393939, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF01842 ACT; PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 32679; Mature: 32679

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERPSIIFRTVCPDRSGLVSQLTSWISNYGGNIKHSDHHTDQEAGLFLSRIEWNLNDLPI
CCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHEEEEEECCCCCCCC
NKVEIYEQFEKNAIDINGQFNINYSDEIPNVGIFVSKQNHCLIDLLWRVRNGELKMNVPL
HHHHHHHHHCCCEEEECCEEEEEECCCCCCCEEEEECCCCCHHHHHHHHCCCCEEEECCE
IISNHPDLEEIAKDFNAQFVYIDNLKYSKSTVENQILNLLNDFDIELVVLAKYMQILSDS
EECCCCCHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
FLKSYSSIINIHHSFLPAFKGAQPYHRAWKRGVKLIGATAHYVTQDLDEGPIIEQCTVNV
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCEEEEHHHHHHHHCCCCCCCCHHEEECC
SHRDEVADLIRKGRDTERIALARAVRLHLNHQIFVYDSKTAVFD
CCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCC
>Mature Secondary Structure
MERPSIIFRTVCPDRSGLVSQLTSWISNYGGNIKHSDHHTDQEAGLFLSRIEWNLNDLPI
CCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHEEEEEECCCCCCCC
NKVEIYEQFEKNAIDINGQFNINYSDEIPNVGIFVSKQNHCLIDLLWRVRNGELKMNVPL
HHHHHHHHHCCCEEEECCEEEEEECCCCCCCEEEEECCCCCHHHHHHHHCCCCEEEECCE
IISNHPDLEEIAKDFNAQFVYIDNLKYSKSTVENQILNLLNDFDIELVVLAKYMQILSDS
EECCCCCHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
FLKSYSSIINIHHSFLPAFKGAQPYHRAWKRGVKLIGATAHYVTQDLDEGPIIEQCTVNV
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCEEEEHHHHHHHHCCCCCCCCHHEEECC
SHRDEVADLIRKGRDTERIALARAVRLHLNHQIFVYDSKTAVFD
CCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]