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The map label for this gene is acnB [H]

Identifier: 123967124

GI number: 123967124

Start: 1681728

End: 1684301

Strand: Direct

Name: acnB [H]

Synonym: P9515_18911

Alternate gene names: 123967124

Gene position: 1681728-1684301 (Clockwise)

Preceding gene: 123967122

Following gene: 123967125

Centisome position: 98.68

GC content: 34.62

Gene sequence:

>2574_bases
ATGAAGAAATTGGAAACATTGCTCAAACATTATGAAGACCACGTAGCTGCAAGAGCTGCTCAAGGCATACCTCCTTTACC
TCTGAATGCCGAGCAGATAAATTGTGTTACCCAACTATTAGAACAAGAAAACAATTTCGAAAGTAGCTATTTACTTGATT
TACTTATCAATAGAGTCCCACCGGGGGTGGATGAAGCAGCATATATAAAAGCAAGCTGGCTTACAGCGATTGTGAATGGA
GAAAAACATTGCAAATTTATTAATGCCCAAAAAGCAATACAGATTTTAGGGACAATGATAGGAGGATATAACGTAAACTC
GCTAATTGAAATACTTAAAAATAAAAATGATTTGCTAGCTAAAGAAGCAGCAAAAGTTTTAAAGAATATTATCCTTGTAT
ACGATGCAGCAAATGATATTTTTGATTTATCTCAAAATAATATTTATGCAGAAGAGGTTGTAAATAGTTGGGCCAATGCA
GAATGGTTTACAACTAAAAAATCCATTCCAAGAGAAATTACATGTTTAGCATTCAAAATCGATGGCGAAACAAACACTGA
TGACTTATCTCCTGCAGTACATGCTACATCACGTCCAGACATCCCATTACACGCATTATCTATGCTTGAATTCAAGAATC
AGGATGGACTAAAAATTCTTGCTGATCTTAAAAAACAAGATCTACAAATAGCGTATGTTGGAGATGTCGTTGGCACAGGC
AGTTCAAGAAAATCTGCCATTAATTCTGTTATTTGGCATTTAGGCGAAGATATCCCATTTGTACCTAATAAAAAAACTGG
TGGAATAATAATAGGAAATAAAATAGCACCAATTTTCTTTAATACAGCCCAAGATTCTGGAGCTCTACCGATTGAAACAG
ATGTCTCTAAAATTAATACTGGTGATTTACTAAAAATATTGCCCTATGAAGGTGTAATAAAAAAAATTGATAAAATTTCA
AATACTGAATTAACAATAAGTCATTTCAACTTGAAACCATCAACACTTACAGATGAAATTCAAGCTGGCGGAAGAATAAA
TCTCATGATTGGGAGATCTCTCACAGATAAAATCAGGAAAAAACTTGAATTACAACCTAGTAAAGTATTTATACGCCCAA
AAAGTCCAAAAGAAATTAATACTGGATTTACTCAAGCACAAAAAATAGTAGGTAAAGCATGTGGATTAAAAGGAGTACTT
CCAGGAATGACATGTGAGCCCATTATGACAACTGTTGGGAGCCAAGATACTACTGGTCCGATGACTAGAGACGAACTTAA
AGAGTTAGCTTGTTTAGGTTTTACTGCAGATTTAGTAATGCAAAGTTTTTGTCATACTGCTGCTTATCCGAAACCAGTTG
ATTTAGTTACTCATAAAGAATTACCTGATTTTATTTCGCAAAGAGGTGGCGTAGCTCTTAAACCTGGTGATGGCATCATT
CATAGTTGGCTTAATAGAATGCTACTTCCAGATACTGTAGGAACAGGAGGAGATAGTCATACGAGATTTCCTCTTGGCAT
TTCCTTCCCTGGAGGTTCGGGGATTGTTGCTTTTGCAGCAGCAATAGGATCAATGCCATTAAACATGCCAGAATCAGTAT
TAGTAAAATTTACAGGGAATTTACTACCAGGAATAACACTACGAGATCTTGTGAATGCAATTCCACTCTTTGCAATAAAA
AAAGGTTTATTAACCGTTGCTAAGGAAAACAAGAAAAATATCTTTAACGGAAAAATTATGGAAATAGAAGGTTTGCCTGA
TTTAAAACTTGAGCAAGCGTTTGAATTAACTGATGCCACTGCTGAACGCTCATGTGCTGGCAGCACAATACTCTTATCGC
ATAGGACAGTAGAAGAATATTTAAAAAGTAATATCTGCCTCTTAGAAAAGATGATTGAAAGCAATTATGAAGATGCAAAA
TCGATATCAAGACGAATCAGTGATATGAAAACGTGGCTAAAAAAACCCGAATTAATTCAGCCCGATAAAAATGCCTCTTA
TGAAGAGATTATAGAAATTGACCTAGCTAAAGTTACTCAACCAATAGTAGCTTGCCCAAATGACCCAGATAATGTAAAAG
AAATTGAAAATGTAGAAAATACTGAGATAGATGAAGTTTTTATCGGATCTTGTATGACTAATATCGGACACTATAGGGCT
GCAGCTAAGGTTCTTGAGGGTATAGAAAAATTAAATTCAAGACTATGGATTTGCCCTCCTACAAAAATGGATGAAGAAAC
ACTTAAAGCTGAAGGATACTATGAAATATTTGAAAAATGTGGCGCAAGATTAGAGTTACCAGGTTGTTCATTATGCATGG
GAAATCAAGCAAGAGTAGATGAAGGTTCAGTTGTTTTCTCCACCAGCACAAGAAATTTTGATAATAGACTTGGTAAAAAT
GCTCAGGTATTTTTAGGAAGTGCTGAGTTGGCAGCAGTTTGTGCTCTTTTAGGAAAAATACCTACAGTAAATGAATATCA
AGATATTACTAAAAACAAAATAAATCCATACTCAGACGAACTTTATCGTTATCTTCAATTTGATGAAATAGAAAATTTCA
GTTTGACAAAATAA

Upstream 100 bases:

>100_bases
TCGTCATTAATTCAGTAGTTTGAATATTTTAAAAAGCATTGATAAATACTCTCCTTATCAATATTAAAAATAACAATAGA
TTAGGCATTAAACCTCACAA

Downstream 100 bases:

>100_bases
TAAAGGACTATGAAAAAGCTAATAAAAGAAAATATTCAAAAAACCAGTAGTAACTCTTCTCAAAGCATAAAAAAATTACT
TAAACAGAGATCTTTTGTTG

Product: bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase

Products: NA

Alternate protein names: Aconitase 2; 2-methylisocitrate dehydratase; Citrate hydro-lyase 2 [H]

Number of amino acids: Translated: 857; Mature: 857

Protein sequence:

>857_residues
MKKLETLLKHYEDHVAARAAQGIPPLPLNAEQINCVTQLLEQENNFESSYLLDLLINRVPPGVDEAAYIKASWLTAIVNG
EKHCKFINAQKAIQILGTMIGGYNVNSLIEILKNKNDLLAKEAAKVLKNIILVYDAANDIFDLSQNNIYAEEVVNSWANA
EWFTTKKSIPREITCLAFKIDGETNTDDLSPAVHATSRPDIPLHALSMLEFKNQDGLKILADLKKQDLQIAYVGDVVGTG
SSRKSAINSVIWHLGEDIPFVPNKKTGGIIIGNKIAPIFFNTAQDSGALPIETDVSKINTGDLLKILPYEGVIKKIDKIS
NTELTISHFNLKPSTLTDEIQAGGRINLMIGRSLTDKIRKKLELQPSKVFIRPKSPKEINTGFTQAQKIVGKACGLKGVL
PGMTCEPIMTTVGSQDTTGPMTRDELKELACLGFTADLVMQSFCHTAAYPKPVDLVTHKELPDFISQRGGVALKPGDGII
HSWLNRMLLPDTVGTGGDSHTRFPLGISFPGGSGIVAFAAAIGSMPLNMPESVLVKFTGNLLPGITLRDLVNAIPLFAIK
KGLLTVAKENKKNIFNGKIMEIEGLPDLKLEQAFELTDATAERSCAGSTILLSHRTVEEYLKSNICLLEKMIESNYEDAK
SISRRISDMKTWLKKPELIQPDKNASYEEIIEIDLAKVTQPIVACPNDPDNVKEIENVENTEIDEVFIGSCMTNIGHYRA
AAKVLEGIEKLNSRLWICPPTKMDEETLKAEGYYEIFEKCGARLELPGCSLCMGNQARVDEGSVVFSTSTRNFDNRLGKN
AQVFLGSAELAAVCALLGKIPTVNEYQDITKNKINPYSDELYRYLQFDEIENFSLTK

Sequences:

>Translated_857_residues
MKKLETLLKHYEDHVAARAAQGIPPLPLNAEQINCVTQLLEQENNFESSYLLDLLINRVPPGVDEAAYIKASWLTAIVNG
EKHCKFINAQKAIQILGTMIGGYNVNSLIEILKNKNDLLAKEAAKVLKNIILVYDAANDIFDLSQNNIYAEEVVNSWANA
EWFTTKKSIPREITCLAFKIDGETNTDDLSPAVHATSRPDIPLHALSMLEFKNQDGLKILADLKKQDLQIAYVGDVVGTG
SSRKSAINSVIWHLGEDIPFVPNKKTGGIIIGNKIAPIFFNTAQDSGALPIETDVSKINTGDLLKILPYEGVIKKIDKIS
NTELTISHFNLKPSTLTDEIQAGGRINLMIGRSLTDKIRKKLELQPSKVFIRPKSPKEINTGFTQAQKIVGKACGLKGVL
PGMTCEPIMTTVGSQDTTGPMTRDELKELACLGFTADLVMQSFCHTAAYPKPVDLVTHKELPDFISQRGGVALKPGDGII
HSWLNRMLLPDTVGTGGDSHTRFPLGISFPGGSGIVAFAAAIGSMPLNMPESVLVKFTGNLLPGITLRDLVNAIPLFAIK
KGLLTVAKENKKNIFNGKIMEIEGLPDLKLEQAFELTDATAERSCAGSTILLSHRTVEEYLKSNICLLEKMIESNYEDAK
SISRRISDMKTWLKKPELIQPDKNASYEEIIEIDLAKVTQPIVACPNDPDNVKEIENVENTEIDEVFIGSCMTNIGHYRA
AAKVLEGIEKLNSRLWICPPTKMDEETLKAEGYYEIFEKCGARLELPGCSLCMGNQARVDEGSVVFSTSTRNFDNRLGKN
AQVFLGSAELAAVCALLGKIPTVNEYQDITKNKINPYSDELYRYLQFDEIENFSLTK
>Mature_857_residues
MKKLETLLKHYEDHVAARAAQGIPPLPLNAEQINCVTQLLEQENNFESSYLLDLLINRVPPGVDEAAYIKASWLTAIVNG
EKHCKFINAQKAIQILGTMIGGYNVNSLIEILKNKNDLLAKEAAKVLKNIILVYDAANDIFDLSQNNIYAEEVVNSWANA
EWFTTKKSIPREITCLAFKIDGETNTDDLSPAVHATSRPDIPLHALSMLEFKNQDGLKILADLKKQDLQIAYVGDVVGTG
SSRKSAINSVIWHLGEDIPFVPNKKTGGIIIGNKIAPIFFNTAQDSGALPIETDVSKINTGDLLKILPYEGVIKKIDKIS
NTELTISHFNLKPSTLTDEIQAGGRINLMIGRSLTDKIRKKLELQPSKVFIRPKSPKEINTGFTQAQKIVGKACGLKGVL
PGMTCEPIMTTVGSQDTTGPMTRDELKELACLGFTADLVMQSFCHTAAYPKPVDLVTHKELPDFISQRGGVALKPGDGII
HSWLNRMLLPDTVGTGGDSHTRFPLGISFPGGSGIVAFAAAIGSMPLNMPESVLVKFTGNLLPGITLRDLVNAIPLFAIK
KGLLTVAKENKKNIFNGKIMEIEGLPDLKLEQAFELTDATAERSCAGSTILLSHRTVEEYLKSNICLLEKMIESNYEDAK
SISRRISDMKTWLKKPELIQPDKNASYEEIIEIDLAKVTQPIVACPNDPDNVKEIENVENTEIDEVFIGSCMTNIGHYRA
AAKVLEGIEKLNSRLWICPPTKMDEETLKAEGYYEIFEKCGARLELPGCSLCMGNQARVDEGSVVFSTSTRNFDNRLGKN
AQVFLGSAELAAVCALLGKIPTVNEYQDITKNKINPYSDELYRYLQFDEIENFSLTK

Specific function: Catalyzes the isomerization of citrate to isocitrate via cis-aconitate as well as the dehydration of 2-methylisocitrate to cis-2-methylaconitate [H]

COG id: COG1049

COG function: function code C; Aconitase B

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family [H]

Homologues:

Organism=Escherichia coli, GI2367097, Length=860, Percent_Identity=58.3720930232558, Blast_Score=999, Evalue=0.0,
Organism=Escherichia coli, GI1786259, Length=371, Percent_Identity=24.5283018867925, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6322261, Length=406, Percent_Identity=24.6305418719212, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6323335, Length=429, Percent_Identity=24.2424242424242, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6321429, Length=401, Percent_Identity=23.6907730673317, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015928
- InterPro:   IPR018136
- InterPro:   IPR004406
- InterPro:   IPR015930
- InterPro:   IPR015933
- InterPro:   IPR015929 [H]

Pfam domain/function: PF00330 Aconitase; PF06434 Aconitase_2_N; PF11791 Aconitase_B_N [H]

EC number: =4.2.1.3; =4.2.1.99 [H]

Molecular weight: Translated: 94385; Mature: 94385

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLETLLKHYEDHVAARAAQGIPPLPLNAEQINCVTQLLEQENNFESSYLLDLLINRVP
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC
PGVDEAAYIKASWLTAIVNGEKHCKFINAQKAIQILGTMIGGYNVNSLIEILKNKNDLLA
CCCCCHHHHHHHHHHHHHCCCHHHEEHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHH
KEAAKVLKNIILVYDAANDIFDLSQNNIYAEEVVNSWANAEWFTTKKSIPREITCLAFKI
HHHHHHHHHHEEEEECCCCHHCCCCCCEEHHHHHHHHCCCCEEEEHHCCCCEEEEEEEEE
DGETNTDDLSPAVHATSRPDIPLHALSMLEFKNQDGLKILADLKKQDLQIAYVGDVVGTG
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCEEEEEECCCCCCC
SSRKSAINSVIWHLGEDIPFVPNKKTGGIIIGNKIAPIFFNTAQDSGALPIETDVSKINT
CHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCEEEEECCCCCCCCCCCCCCCCCCC
GDLLKILPYEGVIKKIDKISNTELTISHFNLKPSTLTDEIQAGGRINLMIGRSLTDKIRK
CCEEEECCCHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHCCCEEEEEECCHHHHHHHH
KLELQPSKVFIRPKSPKEINTGFTQAQKIVGKACGLKGVLPGMTCEPIMTTVGSQDTTGP
HHCCCCCEEEECCCCCCHHHCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCCCC
MTRDELKELACLGFTADLVMQSFCHTAAYPKPVDLVTHKELPDFISQRGGVALKPGDGII
CCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCHH
HSWLNRMLLPDTVGTGGDSHTRFPLGISFPGGSGIVAFAAAIGSMPLNMPESVLVKFTGN
HHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCHHHHHHHHCC
LLPGITLRDLVNAIPLFAIKKGLLTVAKENKKNIFNGKIMEIEGLPDLKLEQAFELTDAT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHH
AERSCAGSTILLSHRTVEEYLKSNICLLEKMIESNYEDAKSISRRISDMKTWLKKPELIQ
HHHHCCCCEEEEEHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCC
PDKNASYEEIIEIDLAKVTQPIVACPNDPDNVKEIENVENTEIDEVFIGSCMTNIGHYRA
CCCCCCHHHHHHHHHHHHCCCCEECCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
AAKVLEGIEKLNSRLWICPPTKMDEETLKAEGYYEIFEKCGARLELPGCSLCMGNQARVD
HHHHHHHHHHHCCCEEEECCCCCCHHHHHCCCHHHHHHHCCCEEECCCCCEECCCCCCCC
EGSVVFSTSTRNFDNRLGKNAQVFLGSAELAAVCALLGKIPTVNEYQDITKNKINPYSDE
CCCEEEEECCCCHHHHHCCCCEEEECCHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCHHH
LYRYLQFDEIENFSLTK
HHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKKLETLLKHYEDHVAARAAQGIPPLPLNAEQINCVTQLLEQENNFESSYLLDLLINRVP
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC
PGVDEAAYIKASWLTAIVNGEKHCKFINAQKAIQILGTMIGGYNVNSLIEILKNKNDLLA
CCCCCHHHHHHHHHHHHHCCCHHHEEHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHH
KEAAKVLKNIILVYDAANDIFDLSQNNIYAEEVVNSWANAEWFTTKKSIPREITCLAFKI
HHHHHHHHHHEEEEECCCCHHCCCCCCEEHHHHHHHHCCCCEEEEHHCCCCEEEEEEEEE
DGETNTDDLSPAVHATSRPDIPLHALSMLEFKNQDGLKILADLKKQDLQIAYVGDVVGTG
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCEEEEEECCCCCCC
SSRKSAINSVIWHLGEDIPFVPNKKTGGIIIGNKIAPIFFNTAQDSGALPIETDVSKINT
CHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCEEEEECCCCCCCCCCCCCCCCCCC
GDLLKILPYEGVIKKIDKISNTELTISHFNLKPSTLTDEIQAGGRINLMIGRSLTDKIRK
CCEEEECCCHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHCCCEEEEEECCHHHHHHHH
KLELQPSKVFIRPKSPKEINTGFTQAQKIVGKACGLKGVLPGMTCEPIMTTVGSQDTTGP
HHCCCCCEEEECCCCCCHHHCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCCCC
MTRDELKELACLGFTADLVMQSFCHTAAYPKPVDLVTHKELPDFISQRGGVALKPGDGII
CCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCHH
HSWLNRMLLPDTVGTGGDSHTRFPLGISFPGGSGIVAFAAAIGSMPLNMPESVLVKFTGN
HHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCHHHHHHHHCC
LLPGITLRDLVNAIPLFAIKKGLLTVAKENKKNIFNGKIMEIEGLPDLKLEQAFELTDAT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHH
AERSCAGSTILLSHRTVEEYLKSNICLLEKMIESNYEDAKSISRRISDMKTWLKKPELIQ
HHHHCCCCEEEEEHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCC
PDKNASYEEIIEIDLAKVTQPIVACPNDPDNVKEIENVENTEIDEVFIGSCMTNIGHYRA
CCCCCCHHHHHHHHHHHHCCCCEECCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
AAKVLEGIEKLNSRLWICPPTKMDEETLKAEGYYEIFEKCGARLELPGCSLCMGNQARVD
HHHHHHHHHHHCCCEEEECCCCCCHHHHHCCCHHHHHHHCCCEEECCCCCEECCCCCCCC
EGSVVFSTSTRNFDNRLGKNAQVFLGSAELAAVCALLGKIPTVNEYQDITKNKINPYSDE
CCCEEEEECCCCHHHHHCCCCEEEECCHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCHHH
LYRYLQFDEIENFSLTK
HHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]