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The map label for this gene is thiG

Identifier: 123967087

GI number: 123967087

Start: 1641838

End: 1642635

Strand: Direct

Name: thiG

Synonym: P9515_18541

Alternate gene names: 123967087

Gene position: 1641838-1642635 (Clockwise)

Preceding gene: 123967086

Following gene: 123967088

Centisome position: 96.34

GC content: 35.34

Gene sequence:

>798_bases
ATGAAAGAAGATTCAGCCTTAATTATTGGAGGTAAAAACTTTTCAAGCCGATTAATGGTAGGCACAGGAAAGTACACATC
TGCAGAAGTAATGGTAGAAAGTTTATTAAATACTGAATCTGAGATAGTAACTGTTGCAGTTAGAAGAGTGCAAAATCATC
AAAATGGTGAAAATTTATTAGAAAAAATTGATTGGGAAAAATTCTGGATGCTTCCTAATACTGCTGGCTGCGCTAATGCA
GATGAAGCAGTGAGAATAGCAATTTTAGGAAGAGAACTTGCAAAATTATCAGGTCAAGAAGAAAATAATTTCGTCAAATT
AGAAGTGATTCCCGATAAAAAATATTTATTACCTGATCCCATAGAAACTCTGAAAGCAGCTGAAATTTTAATAAAAAAAG
ATTTTATTGTTCTACCTTATATAAATGCTGATCCAATATTAGCTAAAAAGTTAGAAGAGATAGGTTGTTCAACAGTAATG
CCATTAGGATCACCTATCGGCTCCGGCCAAGGTCTTTTAAATCTATCTAATATTTCCATAATTATTGAAAATTCCAATAT
TCCAGTAATAATTGATGCTGGTATAGGTGTCCCGAGTGAAGCTTCTCAAGCTATGGAACTGGGAGCAGATGGGGTTTTAA
TCAATAGTGCAATTGCCTTGGCTAAAGACCCTTTAAAAATGGCTAAAGCCATGAATCATGGGGTAAGAGCTGGGAGAGAA
GCCTTTCTGGCTGGAAGAATTGAAAAACAGAGGTTGGCAAGCGCTAGTTCTCCTTTCACAAACATCTCGAAAAAGTAA

Upstream 100 bases:

>100_bases
TAGATCCAAACAACAAAACAGCAATCAAGAAGAGTAAAGAACTACAACAAAGGAATAATTACTCTCCTTATAAAGGAATA
AAAGATAAAGGGTTTTAAAA

Downstream 100 bases:

>100_bases
TATTAAAGCCTTTAAATATTTTATCAAAAACTAAAAAAAAGGTTTATGAAAACTAATTTATATATTGAAAAAAAAGATTT
GAAACTTTTTACAATATTTT

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MKEDSALIIGGKNFSSRLMVGTGKYTSAEVMVESLLNTESEIVTVAVRRVQNHQNGENLLEKIDWEKFWMLPNTAGCANA
DEAVRIAILGRELAKLSGQEENNFVKLEVIPDKKYLLPDPIETLKAAEILIKKDFIVLPYINADPILAKKLEEIGCSTVM
PLGSPIGSGQGLLNLSNISIIIENSNIPVIIDAGIGVPSEASQAMELGADGVLINSAIALAKDPLKMAKAMNHGVRAGRE
AFLAGRIEKQRLASASSPFTNISKK

Sequences:

>Translated_265_residues
MKEDSALIIGGKNFSSRLMVGTGKYTSAEVMVESLLNTESEIVTVAVRRVQNHQNGENLLEKIDWEKFWMLPNTAGCANA
DEAVRIAILGRELAKLSGQEENNFVKLEVIPDKKYLLPDPIETLKAAEILIKKDFIVLPYINADPILAKKLEEIGCSTVM
PLGSPIGSGQGLLNLSNISIIIENSNIPVIIDAGIGVPSEASQAMELGADGVLINSAIALAKDPLKMAKAMNHGVRAGRE
AFLAGRIEKQRLASASSPFTNISKK
>Mature_265_residues
MKEDSALIIGGKNFSSRLMVGTGKYTSAEVMVESLLNTESEIVTVAVRRVQNHQNGENLLEKIDWEKFWMLPNTAGCANA
DEAVRIAILGRELAKLSGQEENNFVKLEVIPDKKYLLPDPIETLKAAEILIKKDFIVLPYINADPILAKKLEEIGCSTVM
PLGSPIGSGQGLLNLSNISIIIENSNIPVIIDAGIGVPSEASQAMELGADGVLINSAIALAKDPLKMAKAMNHGVRAGRE
AFLAGRIEKQRLASASSPFTNISKK

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family

Homologues:

Organism=Escherichia coli, GI48994993, Length=256, Percent_Identity=48.828125, Blast_Score=253, Evalue=1e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIG_PROM5 (A2BZ50)

Other databases:

- EMBL:   CP000552
- RefSeq:   YP_001012168.1
- ProteinModelPortal:   A2BZ50
- SMR:   A2BZ50
- STRING:   A2BZ50
- GeneID:   4720432
- GenomeReviews:   CP000552_GR
- KEGG:   pmc:P9515_18541
- eggNOG:   COG2022
- HOGENOM:   HBG296821
- OMA:   VAIRRTN
- ProtClustDB:   PRK00208
- BioCyc:   PMAR167542:P9515ORF_1936-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00443
- InterPro:   IPR013785
- InterPro:   IPR008867
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF05690 ThiG; SSF110399 ThiG

EC number: NA

Molecular weight: Translated: 28549; Mature: 28549

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: NA

Important sites: ACT_SITE 106-106 BINDING 167-167

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEDSALIIGGKNFSSRLMVGTGKYTSAEVMVESLLNTESEIVTVAVRRVQNHQNGENLL
CCCCCEEEEECCCCCCEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHH
EKIDWEKFWMLPNTAGCANADEAVRIAILGRELAKLSGQEENNFVKLEVIPDKKYLLPDP
HHCCCHHEEECCCCCCCCCCCCEEEEEEEHHHHHHHCCCCCCCEEEEEEECCCCCCCCCH
IETLKAAEILIKKDFIVLPYINADPILAKKLEEIGCSTVMPLGSPIGSGQGLLNLSNISI
HHHHHHHHHHEECCEEEEEECCCCHHHHHHHHHCCCCEECCCCCCCCCCCCEEEEEEEEE
IIENSNIPVIIDAGIGVPSEASQAMELGADGVLINSAIALAKDPLKMAKAMNHGVRAGRE
EEECCCCCEEEECCCCCCCHHHHHHHCCCCCEEEHHHHHHHHCHHHHHHHHHHHHHHHHH
AFLAGRIEKQRLASASSPFTNISKK
HHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MKEDSALIIGGKNFSSRLMVGTGKYTSAEVMVESLLNTESEIVTVAVRRVQNHQNGENLL
CCCCCEEEEECCCCCCEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHH
EKIDWEKFWMLPNTAGCANADEAVRIAILGRELAKLSGQEENNFVKLEVIPDKKYLLPDP
HHCCCHHEEECCCCCCCCCCCCEEEEEEEHHHHHHHCCCCCCCEEEEEEECCCCCCCCCH
IETLKAAEILIKKDFIVLPYINADPILAKKLEEIGCSTVMPLGSPIGSGQGLLNLSNISI
HHHHHHHHHHEECCEEEEEECCCCHHHHHHHHHCCCCEECCCCCCCCCCCCEEEEEEEEE
IIENSNIPVIIDAGIGVPSEASQAMELGADGVLINSAIALAKDPLKMAKAMNHGVRAGRE
EEECCCCCEEEECCCCCCCHHHHHHHCCCCCEEEHHHHHHHHCHHHHHHHHHHHHHHHHH
AFLAGRIEKQRLASASSPFTNISKK
HHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA