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The map label for this gene is mesJ [H]

Identifier: 123967074

GI number: 123967074

Start: 1627104

End: 1628120

Strand: Direct

Name: mesJ [H]

Synonym: P9515_18411

Alternate gene names: 123967074

Gene position: 1627104-1628120 (Clockwise)

Preceding gene: 123967071

Following gene: 123967078

Centisome position: 95.48

GC content: 29.11

Gene sequence:

>1017_bases
ATGTCTAATAAAAATTCAACGCAAAAAAATTGGACATCCTGGCATCATCTCCTACAAAAAGAGATTTTGAGCAATAAAAC
ATTCATTCCAGATGGAGCAAAACTATTAATATCAGTTTCCGGAGGACAGGATTCGATGGCTCTATTAAACCTAATCAATG
ACATGAAAATGCAACATAATTGGTTTGTGAATATTTGGCATGGTGATCATCAATGGCACGAAAAATCTGAAACATATGCA
CTTGAATTGAAAAATTACTGTAATCAAAAAAATATTTCATTTTTTTTTGATCGCGCTAATAAACAAACTATTTCTTCTGA
AGAAAAAGCACGCGATTGGAGATATAAGAAATTAACTGAAAGGGCAAATCAATTATTTATTGAGAACCAAAAAGAAATTG
ATATTTACTTATTAACGGGGCACACTAATACAGATAATGCTGAAACATTTTTACTAAATTTAGCTAGAGGAAGCAATTAT
GCTGGACTAAGTTATATCGAAAAAAAAAGATTGCTCGAACCTCACATTTTTTTAATAAGACCATTGTTAATATTCAGCAG
AGAAGATACTAAGAAATTTTGCCAACTTCAGAAGATTCCTATTTGGGAAGACCCAACTAACTATGATCTAAAAATAAAAA
GAAATTTAATCAGAAAAAAAATTATTCCAACTTTAGAAACTATGTATCCTGGTTGTTCTACAAGGATAAATAATTTTGCT
GAAAAAATGAGAAATTTTAAAAATGAACAAGACGACCTCAGTAAACTTGCATTCCTATCTTGTAAAGATACAAATGGTGT
AAAGAGAGAATTATTAAATGGCTTATGCGTGGAAGCAAGATGTACTATTTTAAATACTTTTATAAAGAAGGATTGTGCAA
AACAATTAAGCTCAAAAAATATAACTTATTTGGTCTCTTCGATTTTTAAAAAACATAGAGGCCAAATAAATTTACCAAAT
GGATTAAAGATTATTTGGGATAAGAATTACATTAATATTGAGAAAACTAGGAAGTAA

Upstream 100 bases:

>100_bases
ATTAATCAGACTCATAGACTTGATGAATTTATTACCTGATTGTGACATTGTTTTATTAAAACAGTGAATTTTTTTAAAAA
TATTATCTGAGTTAAACAAT

Downstream 100 bases:

>100_bases
CTGCCGATCTTCTTCTCCTAGTCCTACCCGCAGGCGCATCTTCCGTCTTAGCTACTGAAGGTGATACAGAATCTTTTTGA
GGTTGGTTATTACTTACTTG

Product: ATPase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MSNKNSTQKNWTSWHHLLQKEILSNKTFIPDGAKLLISVSGGQDSMALLNLINDMKMQHNWFVNIWHGDHQWHEKSETYA
LELKNYCNQKNISFFFDRANKQTISSEEKARDWRYKKLTERANQLFIENQKEIDIYLLTGHTNTDNAETFLLNLARGSNY
AGLSYIEKKRLLEPHIFLIRPLLIFSREDTKKFCQLQKIPIWEDPTNYDLKIKRNLIRKKIIPTLETMYPGCSTRINNFA
EKMRNFKNEQDDLSKLAFLSCKDTNGVKRELLNGLCVEARCTILNTFIKKDCAKQLSSKNITYLVSSIFKKHRGQINLPN
GLKIIWDKNYINIEKTRK

Sequences:

>Translated_338_residues
MSNKNSTQKNWTSWHHLLQKEILSNKTFIPDGAKLLISVSGGQDSMALLNLINDMKMQHNWFVNIWHGDHQWHEKSETYA
LELKNYCNQKNISFFFDRANKQTISSEEKARDWRYKKLTERANQLFIENQKEIDIYLLTGHTNTDNAETFLLNLARGSNY
AGLSYIEKKRLLEPHIFLIRPLLIFSREDTKKFCQLQKIPIWEDPTNYDLKIKRNLIRKKIIPTLETMYPGCSTRINNFA
EKMRNFKNEQDDLSKLAFLSCKDTNGVKRELLNGLCVEARCTILNTFIKKDCAKQLSSKNITYLVSSIFKKHRGQINLPN
GLKIIWDKNYINIEKTRK
>Mature_337_residues
SNKNSTQKNWTSWHHLLQKEILSNKTFIPDGAKLLISVSGGQDSMALLNLINDMKMQHNWFVNIWHGDHQWHEKSETYAL
ELKNYCNQKNISFFFDRANKQTISSEEKARDWRYKKLTERANQLFIENQKEIDIYLLTGHTNTDNAETFLLNLARGSNYA
GLSYIEKKRLLEPHIFLIRPLLIFSREDTKKFCQLQKIPIWEDPTNYDLKIKRNLIRKKIIPTLETMYPGCSTRINNFAE
KMRNFKNEQDDLSKLAFLSCKDTNGVKRELLNGLCVEARCTILNTFIKKDCAKQLSSKNITYLVSSIFKKHRGQINLPNG
LKIIWDKNYINIEKTRK

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=200, Percent_Identity=26.5, Blast_Score=72, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 39756; Mature: 39625

Theoretical pI: Translated: 10.03; Mature: 10.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNKNSTQKNWTSWHHLLQKEILSNKTFIPDGAKLLISVSGGQDSMALLNLINDMKMQHN
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCC
WFVNIWHGDHQWHEKSETYALELKNYCNQKNISFFFDRANKQTISSEEKARDWRYKKLTE
EEEEEEECCCCHHCCCCCEEEEHHHHCCCCCCEEEEECCCCHHCCCHHHHHHHHHHHHHH
RANQLFIENQKEIDIYLLTGHTNTDNAETFLLNLARGSNYAGLSYIEKKRLLEPHIFLIR
HHHHEEECCCCEEEEEEEECCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCHHHHHH
PLLIFSREDTKKFCQLQKIPIWEDPTNYDLKIKRNLIRKKIIPTLETMYPGCSTRINNFA
HHHHHCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
EKMRNFKNEQDDLSKLAFLSCKDTNGVKRELLNGLCVEARCTILNTFIKKDCAKQLSSKN
HHHHHHCCCHHHHHHHHEEECCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCC
ITYLVSSIFKKHRGQINLPNGLKIIWDKNYINIEKTRK
HHHHHHHHHHHHCCCEECCCCCEEEEECCEEECCCCCC
>Mature Secondary Structure 
SNKNSTQKNWTSWHHLLQKEILSNKTFIPDGAKLLISVSGGQDSMALLNLINDMKMQHN
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCC
WFVNIWHGDHQWHEKSETYALELKNYCNQKNISFFFDRANKQTISSEEKARDWRYKKLTE
EEEEEEECCCCHHCCCCCEEEEHHHHCCCCCCEEEEECCCCHHCCCHHHHHHHHHHHHHH
RANQLFIENQKEIDIYLLTGHTNTDNAETFLLNLARGSNYAGLSYIEKKRLLEPHIFLIR
HHHHEEECCCCEEEEEEEECCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCHHHHHH
PLLIFSREDTKKFCQLQKIPIWEDPTNYDLKIKRNLIRKKIIPTLETMYPGCSTRINNFA
HHHHHCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
EKMRNFKNEQDDLSKLAFLSCKDTNGVKRELLNGLCVEARCTILNTFIKKDCAKQLSSKN
HHHHHHCCCHHHHHHHHEEECCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCC
ITYLVSSIFKKHRGQINLPNGLKIIWDKNYINIEKTRK
HHHHHHHHHHHHCCCEECCCCCEEEEECCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]