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The map label for this gene is recF [H]

Identifier: 123966993

GI number: 123966993

Start: 1545297

End: 1546274

Strand: Reverse

Name: recF [H]

Synonym: P9515_17601

Alternate gene names: 123966993

Gene position: 1546274-1545297 (Counterclockwise)

Preceding gene: 123966994

Following gene: 123966992

Centisome position: 90.73

GC content: 30.98

Gene sequence:

>978_bases
TTGCTTGAATCAGTTGAAGTTCTTAGCCAATTAAGATCTAGTAGAGCATTAAGTGATAAGGATTTAATAAAAAATGATAG
CGAGATGGCAGTTATATATGGCCAAATCGATTTTACTGATGATTTGAAAGTGAATTTATTTAGGAAAGGATCTAAAAAGA
TTTATGTTAATGATTCTTTATTGAAAAAACAGAGTGAAATTAAAAATTATATTAGAAGTGTATGTTTCTGCTCAAATGAT
ATCAACATTGTTAAAAGTGAACCTGGCTATAGAAGAACTTGGATTGATAGAGTTGTATCTCAGCTTGAACCGATATATGT
AGAACTGATTCATAGATTTAATAGACTTTTAAAACAAAGAAGTTATTTTTGGCGTTCAGAAAGCTTTCAAAAAGATCAAT
CTTCAGAAGTTATTGAAAGTTTTGATATTCAAATGTCATTAATTTGCACAAGAATTTTTAGGCGTAGGAGAAGAGCAATA
TCAAGAATTAGACCGTATGTTGAATATTGGCATAATCATTTAAGTAAATCCAAAGAGCAAATCAGTATAAATTATCTTTC
TAGTTTTGAAAATATAGATGAGGCTGAAGAAGAAGAAGAAGTGATAAGTAATCAAATGGTGGACCAATTACAAAAGCAGA
GGGCAATAGAGGCATTAACTGGTAAATGTAGCTTTGGTCCTCATCGTGATGATATCGAATTTCTTATCAATGATATCTCT
TTAAGGAAATATGGCTCATCTGGGCAGCAAAGGACTTTTATTTTGGCTCTAAAAATGGCTGAACTCGATTTATTGCGTAA
CATGATAAATTTACCTCCCCTTCTGATATTAGATGATGTCTTGGCTGAGCTTGACATGAATAGGCAAAATTTGTTATTAA
ATTCGGTAGGGAAAGAGAGTCAATGTTTTATAAGTGCGACGCATTTAGATACATTCAATCAATCTTTTATAAGCTCATCA
CAAATGATTCATTTATAA

Upstream 100 bases:

>100_bases
CTTAAAAATAAAAAATTTTCGAAACCATAAAAGTTTTGAGATAGATCTTCATGAGCAAAGAGCTATTATTCTTGGTTGTA
ATGGTATAGGTAAGTCGAAT

Downstream 100 bases:

>100_bases
TGGAAAAGTTGTAATTTTTACCTAACTTAAAAAATATATTAATGGATGTTCCCAAAAAAAATAAAACTTTTAAAGCATTT
AATGATGAGAAGAAATTAAT

Product: putative DNA repair and genetic recombination protein RecF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MLESVEVLSQLRSSRALSDKDLIKNDSEMAVIYGQIDFTDDLKVNLFRKGSKKIYVNDSLLKKQSEIKNYIRSVCFCSND
INIVKSEPGYRRTWIDRVVSQLEPIYVELIHRFNRLLKQRSYFWRSESFQKDQSSEVIESFDIQMSLICTRIFRRRRRAI
SRIRPYVEYWHNHLSKSKEQISINYLSSFENIDEAEEEEEVISNQMVDQLQKQRAIEALTGKCSFGPHRDDIEFLINDIS
LRKYGSSGQQRTFILALKMAELDLLRNMINLPPLLILDDVLAELDMNRQNLLLNSVGKESQCFISATHLDTFNQSFISSS
QMIHL

Sequences:

>Translated_325_residues
MLESVEVLSQLRSSRALSDKDLIKNDSEMAVIYGQIDFTDDLKVNLFRKGSKKIYVNDSLLKKQSEIKNYIRSVCFCSND
INIVKSEPGYRRTWIDRVVSQLEPIYVELIHRFNRLLKQRSYFWRSESFQKDQSSEVIESFDIQMSLICTRIFRRRRRAI
SRIRPYVEYWHNHLSKSKEQISINYLSSFENIDEAEEEEEVISNQMVDQLQKQRAIEALTGKCSFGPHRDDIEFLINDIS
LRKYGSSGQQRTFILALKMAELDLLRNMINLPPLLILDDVLAELDMNRQNLLLNSVGKESQCFISATHLDTFNQSFISSS
QMIHL
>Mature_325_residues
MLESVEVLSQLRSSRALSDKDLIKNDSEMAVIYGQIDFTDDLKVNLFRKGSKKIYVNDSLLKKQSEIKNYIRSVCFCSND
INIVKSEPGYRRTWIDRVVSQLEPIYVELIHRFNRLLKQRSYFWRSESFQKDQSSEVIESFDIQMSLICTRIFRRRRRAI
SRIRPYVEYWHNHLSKSKEQISINYLSSFENIDEAEEEEEVISNQMVDQLQKQRAIEALTGKCSFGPHRDDIEFLINDIS
LRKYGSSGQQRTFILALKMAELDLLRNMINLPPLLILDDVLAELDMNRQNLLLNSVGKESQCFISATHLDTFNQSFISSS
QMIHL

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP [H]

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001238
- InterPro:   IPR018078 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 38083; Mature: 38083

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: PS00617 RECF_1 ; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLESVEVLSQLRSSRALSDKDLIKNDSEMAVIYGQIDFTDDLKVNLFRKGSKKIYVNDSL
CCCHHHHHHHHHHHCCCCHHHHHCCCCCEEEEEEEEECCCCCEEEEEECCCCEEEECHHH
LKKQSEIKNYIRSVCFCSNDINIVKSEPGYRRTWIDRVVSQLEPIYVELIHRFNRLLKQR
HHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SYFWRSESFQKDQSSEVIESFDIQMSLICTRIFRRRRRAISRIRPYVEYWHNHLSKSKEQ
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
ISINYLSSFENIDEAEEEEEVISNQMVDQLQKQRAIEALTGKCSFGPHRDDIEFLINDIS
EEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
LRKYGSSGQQRTFILALKMAELDLLRNMINLPPLLILDDVLAELDMNRQNLLLNSVGKES
HHHCCCCCCCEEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCC
QCFISATHLDTFNQSFISSSQMIHL
CEEEEEHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MLESVEVLSQLRSSRALSDKDLIKNDSEMAVIYGQIDFTDDLKVNLFRKGSKKIYVNDSL
CCCHHHHHHHHHHHCCCCHHHHHCCCCCEEEEEEEEECCCCCEEEEEECCCCEEEECHHH
LKKQSEIKNYIRSVCFCSNDINIVKSEPGYRRTWIDRVVSQLEPIYVELIHRFNRLLKQR
HHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SYFWRSESFQKDQSSEVIESFDIQMSLICTRIFRRRRRAISRIRPYVEYWHNHLSKSKEQ
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
ISINYLSSFENIDEAEEEEEVISNQMVDQLQKQRAIEALTGKCSFGPHRDDIEFLINDIS
EEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
LRKYGSSGQQRTFILALKMAELDLLRNMINLPPLLILDDVLAELDMNRQNLLLNSVGKES
HHHCCCCCCCEEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCC
QCFISATHLDTFNQSFISSSQMIHL
CEEEEEHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917641 [H]