Click here to switch to the map view.

The map label for this gene is prfA [H]

Identifier: 123966944

GI number: 123966944

Start: 1513017

End: 1514111

Strand: Reverse

Name: prfA [H]

Synonym: P9515_17111

Alternate gene names: 123966944

Gene position: 1514111-1513017 (Counterclockwise)

Preceding gene: 123966945

Following gene: 123966942

Centisome position: 88.85

GC content: 35.16

Gene sequence:

>1095_bases
TTGGAATACACAACATTAATTGCAAGGTTAAAAACTGCTTCAAAAAGTTTTGTGAATTTAGAAATGCAGTTGGCAGATCC
CGATATTGCAAATAATCCAAAAAAATTAGAATTAATAGCAAAAGAAAGAGCCAAACTAGAACCTTTGGTTCTTGATTTTA
ATCAATTGCTTGATACTGATAAAGAAATTGACGATTCCAGACAACTGCTCAAAGATAATAGGAATGATAAGGATATGGAA
TTGTTGATTAATGAAGAATTATGTAATCTAGAAGTTCTAAAGAATTCACTTATCCAAAAAGTAACAATAGCTTTACTTCC
AAAAGATCCTCGAGATGAAAGAAGTGTAATGCTTGAGATAAGAGCTGGCGCTGGAGGGAATGAAGCTTGTATTTGGGCTG
GTGATTTAGCAAGAATGTATGAACGATATGGACAAAAAATCGGGTGGTCAGTAAAACCTATTAGTGCCTCAGAATCTGAT
ATGGGAGGATTTAAAGAATTAGTTATTTCTATAAAAGGAGATTCTGTTTATAGTCAATTAAAATTTGAGGCAGGTGTACA
TAGAGTTCAAAGAGTTCCAGCGACGGAATCTCAAGGAAGAGTTCATACTTCTACAGCTACAGTTGCCGTTATGCCTGAGG
CTGATCCAGTTGAAGTTAAGATTGATCCAAGTGATTTAGAAATTGGTACAGCGAGGTCAGGTGGTGCCGGAGGCCAAAAT
GTTAACAAGGTAGAAACTGCTATTGACCTTATTCATAAGCCGACAGGTATAAGAGTGTTTTGTACTCAAGAAAGATCGCA
GCTACAGAACCGTGAGAGAGCCATGGAAATTTTAAGAGCCAAATTATACGAAATTCAATTAAAAGAAGCTAATGATAAGG
AAAGGTCACAAAGGTTAATGCAAGTTGGAACTGGAGATAGAAGCGAAAAAATTAGAACTTATAATTTTAAGGATAATAGA
ACCACTGATCATAGATTAGGCTCTAATTTTGCATTAGAGCCAATTCTGGCAGGACAATTAGATGAAGTTATAGATGCTTG
TATAGCTCAAGAACAAAAGAGGATAATGGAGGATTTTAATGAGAATGAGAATTAA

Upstream 100 bases:

>100_bases
GGAATGGGTTCAGCTGATTCCGCTACTTCGCAAGAAACAAAAGAAGCTAAGGAATCAGATAAATAGAAATTTACAAATCT
AAATTTAAGCAAAACTTCAA

Downstream 100 bases:

>100_bases
ATTTTTTTAGTTTTTAGCAGCTAAACCGATCACATATTTACTCCACTCTTTGTGTTTCCCAGAGTCTATTTGTCTCGTCG
CTTCAAAATTCAGATTGCTT

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MEYTTLIARLKTASKSFVNLEMQLADPDIANNPKKLELIAKERAKLEPLVLDFNQLLDTDKEIDDSRQLLKDNRNDKDME
LLINEELCNLEVLKNSLIQKVTIALLPKDPRDERSVMLEIRAGAGGNEACIWAGDLARMYERYGQKIGWSVKPISASESD
MGGFKELVISIKGDSVYSQLKFEAGVHRVQRVPATESQGRVHTSTATVAVMPEADPVEVKIDPSDLEIGTARSGGAGGQN
VNKVETAIDLIHKPTGIRVFCTQERSQLQNRERAMEILRAKLYEIQLKEANDKERSQRLMQVGTGDRSEKIRTYNFKDNR
TTDHRLGSNFALEPILAGQLDEVIDACIAQEQKRIMEDFNENEN

Sequences:

>Translated_364_residues
MEYTTLIARLKTASKSFVNLEMQLADPDIANNPKKLELIAKERAKLEPLVLDFNQLLDTDKEIDDSRQLLKDNRNDKDME
LLINEELCNLEVLKNSLIQKVTIALLPKDPRDERSVMLEIRAGAGGNEACIWAGDLARMYERYGQKIGWSVKPISASESD
MGGFKELVISIKGDSVYSQLKFEAGVHRVQRVPATESQGRVHTSTATVAVMPEADPVEVKIDPSDLEIGTARSGGAGGQN
VNKVETAIDLIHKPTGIRVFCTQERSQLQNRERAMEILRAKLYEIQLKEANDKERSQRLMQVGTGDRSEKIRTYNFKDNR
TTDHRLGSNFALEPILAGQLDEVIDACIAQEQKRIMEDFNENEN
>Mature_364_residues
MEYTTLIARLKTASKSFVNLEMQLADPDIANNPKKLELIAKERAKLEPLVLDFNQLLDTDKEIDDSRQLLKDNRNDKDME
LLINEELCNLEVLKNSLIQKVTIALLPKDPRDERSVMLEIRAGAGGNEACIWAGDLARMYERYGQKIGWSVKPISASESD
MGGFKELVISIKGDSVYSQLKFEAGVHRVQRVPATESQGRVHTSTATVAVMPEADPVEVKIDPSDLEIGTARSGGAGGQN
VNKVETAIDLIHKPTGIRVFCTQERSQLQNRERAMEILRAKLYEIQLKEANDKERSQRLMQVGTGDRSEKIRTYNFKDNR
TTDHRLGSNFALEPILAGQLDEVIDACIAQEQKRIMEDFNENEN

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=306, Percent_Identity=41.8300653594771, Blast_Score=243, Evalue=2e-64,
Organism=Homo sapiens, GI34577120, Length=333, Percent_Identity=35.7357357357357, Blast_Score=219, Evalue=4e-57,
Organism=Homo sapiens, GI166795305, Length=211, Percent_Identity=38.8625592417062, Blast_Score=159, Evalue=3e-39,
Organism=Escherichia coli, GI1787462, Length=361, Percent_Identity=40.7202216066482, Blast_Score=271, Evalue=3e-74,
Organism=Escherichia coli, GI2367172, Length=328, Percent_Identity=35.3658536585366, Blast_Score=195, Evalue=4e-51,
Organism=Caenorhabditis elegans, GI17542784, Length=334, Percent_Identity=34.4311377245509, Blast_Score=186, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6321295, Length=253, Percent_Identity=43.8735177865613, Blast_Score=191, Evalue=1e-49,
Organism=Drosophila melanogaster, GI19921226, Length=310, Percent_Identity=37.741935483871, Blast_Score=204, Evalue=6e-53,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40956; Mature: 40956

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEYTTLIARLKTASKSFVNLEMQLADPDIANNPKKLELIAKERAKLEPLVLDFNQLLDTD
CCHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHCCCHHEECHHHHHCCC
KEIDDSRQLLKDNRNDKDMELLINEELCNLEVLKNSLIQKVTIALLPKDPRDERSVMLEI
CCCHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHEECCCCCCCCCEEEEEE
RAGAGGNEACIWAGDLARMYERYGQKIGWSVKPISASESDMGGFKELVISIKGDSVYSQL
ECCCCCCCEEEEHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCEEEEEEECCCHHHHHH
KFEAGVHRVQRVPATESQGRVHTSTATVAVMPEADPVEVKIDPSDLEIGTARSGGAGGQN
HHHHHHHHHHCCCCCCCCCEEEECCEEEEEECCCCCEEEEECCCCCEEECCCCCCCCCCC
VNKVETAIDLIHKPTGIRVFCTQERSQLQNRERAMEILRAKLYEIQLKEANDKERSQRLM
HHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHH
QVGTGDRSEKIRTYNFKDNRTTDHRLGSNFALEPILAGQLDEVIDACIAQEQKRIMEDFN
HCCCCCCCCCEEEECCCCCCCCHHHCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHCC
ENEN
CCCC
>Mature Secondary Structure
MEYTTLIARLKTASKSFVNLEMQLADPDIANNPKKLELIAKERAKLEPLVLDFNQLLDTD
CCHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHCCCHHEECHHHHHCCC
KEIDDSRQLLKDNRNDKDMELLINEELCNLEVLKNSLIQKVTIALLPKDPRDERSVMLEI
CCCHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHEECCCCCCCCCEEEEEE
RAGAGGNEACIWAGDLARMYERYGQKIGWSVKPISASESDMGGFKELVISIKGDSVYSQL
ECCCCCCCEEEEHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCEEEEEEECCCHHHHHH
KFEAGVHRVQRVPATESQGRVHTSTATVAVMPEADPVEVKIDPSDLEIGTARSGGAGGQN
HHHHHHHHHHCCCCCCCCCEEEECCEEEEEECCCCCEEEEECCCCCEEECCCCCCCCCCC
VNKVETAIDLIHKPTGIRVFCTQERSQLQNRERAMEILRAKLYEIQLKEANDKERSQRLM
HHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHH
QVGTGDRSEKIRTYNFKDNRTTDHRLGSNFALEPILAGQLDEVIDACIAQEQKRIMEDFN
HCCCCCCCCCEEEECCCCCCCCHHHCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHCC
ENEN
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA