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The map label for this gene is cobJ [H]

Identifier: 123966937

GI number: 123966937

Start: 1504223

End: 1506037

Strand: Direct

Name: cobJ [H]

Synonym: P9515_17041

Alternate gene names: 123966937

Gene position: 1504223-1506037 (Clockwise)

Preceding gene: 123966933

Following gene: 123966938

Centisome position: 88.27

GC content: 32.34

Gene sequence:

>1815_bases
TTGAAAGGTATTGCAATAGGTTTTTCAGATGCTTCAAAAGAGATTTTAAAAAGACTAAAAAAAACTGAATTTATTAATGA
GATTTATATCGGAAGCTCATCTCAAATAGATAAAAAAATCAAAGATTCAATTGAGTGCTTGAAACCCAAGCTAATTCTGA
AAAAAAAATGGAAAGAATTGGAGTTAATTATCTTTGTCGGGTCAGTAGGAGCCTCAATCAGACTTATCAATTCTTTATTA
TCTTCAAAAGATAAAGATCCTGGAATAATTGTGATTGATAAAAAAGGATCAAAAATCATTCCATTGATTGGAGCACATCA
ATCAAATGTACAAAATATTGCATTTCAAATATGTAATTTATTTGGTGGTGAAATTATAGAAACTAATAATTCAATTGAGG
AAAATTACTTAAATATTGATTCATTTGGATATCAATGGGGATGGAAAAGGTCTGGAGATACAAAAGACTGGTCAAAACTG
GTAATAAAGCAAGCCAATAATAAAGAAATTTTTTGTAGCCAATCCTCAGGCAATGATTTATGGAAAACTTCAGACTCAGG
AAAAAATATTACTCAAATTTCAGAGAAAGATAATGAGCCAAATAAAGCATCCTTTAATATAAGTATATTTAGTAATCATA
AAAATACTTGGCATCCCGCCATGTTATGGATCGGTTTAGGTTGCGAGAGAAATACTAGCAAACAACTAATTGAAGACTCC
ATACAAAAATTTTTAGCAACTAATAATTTATCTCCATTATCAATTGCAGGATATGCAACCATCGATATTAAAAAAGATGA
GCAAGCATTTTTAAAAATCTCACGAGAAAACAAATTGCCTATAAAATTTTTCAGGGCAGAGCTACTATCTACTATTAACG
TACCTAATCCATCAAATATAGTACTTAATGAGATTGGTACACCTTCAGTAGCTGAAGCCGCTTGCATTTTAGCTGCTGGA
GAAGGAGCAAAACTATTGGGTGAAAAGAAAATCTTTAAAAATATCGATCTTTCAAAAAGTAAATTTGGTGCGGTAACGAT
AGCCGTAGCTCGGTCTAAAAATCAATATTACCCACTAACAGGCGAAATTCATGTTGTAGGTATTGGACCGGGAGATATTT
CCTATTTAACAAATGATGCTCGAAAAGCCTTATCAAAATGTTCTATATGGATTGGATATAAAATGTATTTGGATCTAATT
AAACCGCTTAAAAGAAAAGATCAGGTAATTATTGAAAGCAATCTTACTGAAGAAAGAGAGAGATGTGAGAAAGCCATAAA
ACTTTCTGAGGAAGGAATAAAAGTAGCACTTATATCCTCTGGGGATGCAGGTGTTTATGGAATGGCTGGTCTGCTTTTGG
AATTAATTCAAACAATGAAAAAAGAATTAAGGCCCTCATTTGAAATACATCCTGGAATCAGTAGCATGCAACTAGCAGCT
GCACTTGGTGGTGCTCCATTAATGAACGACTTCTGTTCAATAAGCTTGAGTGATAAGTTAACCCCATGGGAATCCATTGA
AAAAAGAATTGAGGGTGCTTTATTGGGAGACTTTGTAATTACCATTTTCAATCCACAATCAATCGAAAGAAATTGGCAAT
TGAAAAGAGCTATAAAATTAGTCTTAGGTCATAGACCTGGAAATACTCCAGTATTAGTAGGCAGACAAGTAGGAAGGAAA
AATCAATCAAAGAGATTTTTTAGTTTGGATAGCATCCCTTTAGAAGAAATAGATATGCTTTCAATAATCATTATTGGCAA
TTCTAAAACTACCTTAGTTGATGATATATTTTTAACCCCAAGAGGATATCTTTGA

Upstream 100 bases:

>100_bases
TTCATAAAAATCTTAAATTTTTTTTTATATTTCATTCCTTATTTGTTAATTACTGCAACAAGATATAATTTCTAGAAGAT
ATCAAAAAGCACTTCTTAAA

Downstream 100 bases:

>100_bases
ACACTAATATCAAAAAATCTCATGTTTAATAATAATTTTTTTTGTTTTTGCTTTTTGTGTAAGAGAATATTAATCTTTAA
TAAATACTATTGAATTTAAT

Product: bifunctional cbiH protein and precorrin-3B C17-methyltransferase

Products: S-adenosyl-L-homocysteine; Precorrin 4

Alternate protein names: Cobalt-precorrin-3 methylase; Cobalt-precorrin-3 methyltransferase [H]

Number of amino acids: Translated: 604; Mature: 604

Protein sequence:

>604_residues
MKGIAIGFSDASKEILKRLKKTEFINEIYIGSSSQIDKKIKDSIECLKPKLILKKKWKELELIIFVGSVGASIRLINSLL
SSKDKDPGIIVIDKKGSKIIPLIGAHQSNVQNIAFQICNLFGGEIIETNNSIEENYLNIDSFGYQWGWKRSGDTKDWSKL
VIKQANNKEIFCSQSSGNDLWKTSDSGKNITQISEKDNEPNKASFNISIFSNHKNTWHPAMLWIGLGCERNTSKQLIEDS
IQKFLATNNLSPLSIAGYATIDIKKDEQAFLKISRENKLPIKFFRAELLSTINVPNPSNIVLNEIGTPSVAEAACILAAG
EGAKLLGEKKIFKNIDLSKSKFGAVTIAVARSKNQYYPLTGEIHVVGIGPGDISYLTNDARKALSKCSIWIGYKMYLDLI
KPLKRKDQVIIESNLTEERERCEKAIKLSEEGIKVALISSGDAGVYGMAGLLLELIQTMKKELRPSFEIHPGISSMQLAA
ALGGAPLMNDFCSISLSDKLTPWESIEKRIEGALLGDFVITIFNPQSIERNWQLKRAIKLVLGHRPGNTPVLVGRQVGRK
NQSKRFFSLDSIPLEEIDMLSIIIIGNSKTTLVDDIFLTPRGYL

Sequences:

>Translated_604_residues
MKGIAIGFSDASKEILKRLKKTEFINEIYIGSSSQIDKKIKDSIECLKPKLILKKKWKELELIIFVGSVGASIRLINSLL
SSKDKDPGIIVIDKKGSKIIPLIGAHQSNVQNIAFQICNLFGGEIIETNNSIEENYLNIDSFGYQWGWKRSGDTKDWSKL
VIKQANNKEIFCSQSSGNDLWKTSDSGKNITQISEKDNEPNKASFNISIFSNHKNTWHPAMLWIGLGCERNTSKQLIEDS
IQKFLATNNLSPLSIAGYATIDIKKDEQAFLKISRENKLPIKFFRAELLSTINVPNPSNIVLNEIGTPSVAEAACILAAG
EGAKLLGEKKIFKNIDLSKSKFGAVTIAVARSKNQYYPLTGEIHVVGIGPGDISYLTNDARKALSKCSIWIGYKMYLDLI
KPLKRKDQVIIESNLTEERERCEKAIKLSEEGIKVALISSGDAGVYGMAGLLLELIQTMKKELRPSFEIHPGISSMQLAA
ALGGAPLMNDFCSISLSDKLTPWESIEKRIEGALLGDFVITIFNPQSIERNWQLKRAIKLVLGHRPGNTPVLVGRQVGRK
NQSKRFFSLDSIPLEEIDMLSIIIIGNSKTTLVDDIFLTPRGYL
>Mature_604_residues
MKGIAIGFSDASKEILKRLKKTEFINEIYIGSSSQIDKKIKDSIECLKPKLILKKKWKELELIIFVGSVGASIRLINSLL
SSKDKDPGIIVIDKKGSKIIPLIGAHQSNVQNIAFQICNLFGGEIIETNNSIEENYLNIDSFGYQWGWKRSGDTKDWSKL
VIKQANNKEIFCSQSSGNDLWKTSDSGKNITQISEKDNEPNKASFNISIFSNHKNTWHPAMLWIGLGCERNTSKQLIEDS
IQKFLATNNLSPLSIAGYATIDIKKDEQAFLKISRENKLPIKFFRAELLSTINVPNPSNIVLNEIGTPSVAEAACILAAG
EGAKLLGEKKIFKNIDLSKSKFGAVTIAVARSKNQYYPLTGEIHVVGIGPGDISYLTNDARKALSKCSIWIGYKMYLDLI
KPLKRKDQVIIESNLTEERERCEKAIKLSEEGIKVALISSGDAGVYGMAGLLLELIQTMKKELRPSFEIHPGISSMQLAA
ALGGAPLMNDFCSISLSDKLTPWESIEKRIEGALLGDFVITIFNPQSIERNWQLKRAIKLVLGHRPGNTPVLVGRQVGRK
NQSKRFFSLDSIPLEEIDMLSIIIIGNSKTTLVDDIFLTPRGYL

Specific function: Methyltransferase that catalyzes the methylation of C-17 in cobalt-precorrin-3B to form cobalt-precorrin-4 [H]

COG id: COG1010

COG function: function code H; Precorrin-3B methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006363 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.131

Molecular weight: Translated: 67077; Mature: 67077

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGIAIGFSDASKEILKRLKKTEFINEIYIGSSSQIDKKIKDSIECLKPKLILKKKWKEL
CCCEEEECCHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHCCHHHHHHCCCEE
ELIIFVGSVGASIRLINSLLSSKDKDPGIIVIDKKGSKIIPLIGAHQSNVQNIAFQICNL
EEEEEEECCCHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEEECCCCCHHHHHHHHHHHH
FGGEIIETNNSIEENYLNIDSFGYQWGWKRSGDTKDWSKLVIKQANNKEIFCSQSSGNDL
CCCEEEECCCCHHHCEEECCCCCCCCCCCCCCCCCHHHHHHHCCCCCCEEEEECCCCCCC
WKTSDSGKNITQISEKDNEPNKASFNISIFSNHKNTWHPAMLWIGLGCERNTSKQLIEDS
CCCCCCCCCEEECCCCCCCCCCEEEEEEEEECCCCCCCCEEEEEECCCCCCCHHHHHHHH
IQKFLATNNLSPLSIAGYATIDIKKDEQAFLKISRENKLPIKFFRAELLSTINVPNPSNI
HHHHHHHCCCCCEEEEEEEEEEECCCCHHHEEEECCCCCCHHHHHHHHHHHCCCCCCCCE
VLNEIGTPSVAEAACILAAGEGAKLLGEKKIFKNIDLSKSKFGAVTIAVARSKNQYYPLT
EEECCCCCCHHHEEEEEECCCCHHHHCCHHHHHCCCCCCCCCCEEEEEEEECCCCEECCC
GEIHVVGIGPGDISYLTNDARKALSKCSIWIGYKMYLDLIKPLKRKDQVIIESNLTEERE
CCEEEEEECCCCHHHHHHHHHHHHHHCEEEEHHHHHHHHHHHHCCCCCEEEECCCHHHHH
RCEKAIKLSEEGIKVALISSGDAGVYGMAGLLLELIQTMKKELRPSFEIHPGISSMQLAA
HHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHH
ALGGAPLMNDFCSISLSDKLTPWESIEKRIEGALLGDFVITIFNPQSIERNWQLKRAIKL
HHCCCHHHHHHHCEEECCCCCCHHHHHHHHCCCEEEEEEEEEECCHHCCCCHHHHHHHHH
VLGHRPGNTPVLVGRQVGRKNQSKRFFSLDSIPLEEIDMLSIIIIGNSKTTLVDDIFLTP
HHCCCCCCCCEEEEHHHCCCCCCCEEEEECCCCHHCCCEEEEEEECCCCCEEEEEEEECC
RGYL
CCCC
>Mature Secondary Structure
MKGIAIGFSDASKEILKRLKKTEFINEIYIGSSSQIDKKIKDSIECLKPKLILKKKWKEL
CCCEEEECCHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHCCHHHHHHCCCEE
ELIIFVGSVGASIRLINSLLSSKDKDPGIIVIDKKGSKIIPLIGAHQSNVQNIAFQICNL
EEEEEEECCCHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEEECCCCCHHHHHHHHHHHH
FGGEIIETNNSIEENYLNIDSFGYQWGWKRSGDTKDWSKLVIKQANNKEIFCSQSSGNDL
CCCEEEECCCCHHHCEEECCCCCCCCCCCCCCCCCHHHHHHHCCCCCCEEEEECCCCCCC
WKTSDSGKNITQISEKDNEPNKASFNISIFSNHKNTWHPAMLWIGLGCERNTSKQLIEDS
CCCCCCCCCEEECCCCCCCCCCEEEEEEEEECCCCCCCCEEEEEECCCCCCCHHHHHHHH
IQKFLATNNLSPLSIAGYATIDIKKDEQAFLKISRENKLPIKFFRAELLSTINVPNPSNI
HHHHHHHCCCCCEEEEEEEEEEECCCCHHHEEEECCCCCCHHHHHHHHHHHCCCCCCCCE
VLNEIGTPSVAEAACILAAGEGAKLLGEKKIFKNIDLSKSKFGAVTIAVARSKNQYYPLT
EEECCCCCCHHHEEEEEECCCCHHHHCCHHHHHCCCCCCCCCCEEEEEEEECCCCEECCC
GEIHVVGIGPGDISYLTNDARKALSKCSIWIGYKMYLDLIKPLKRKDQVIIESNLTEERE
CCEEEEEECCCCHHHHHHHHHHHHHHCEEEEHHHHHHHHHHHHCCCCCEEEECCCHHHHH
RCEKAIKLSEEGIKVALISSGDAGVYGMAGLLLELIQTMKKELRPSFEIHPGISSMQLAA
HHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHH
ALGGAPLMNDFCSISLSDKLTPWESIEKRIEGALLGDFVITIFNPQSIERNWQLKRAIKL
HHCCCHHHHHHHCEEECCCCCCHHHHHHHHCCCEEEEEEEEEECCHHCCCCHHHHHHHHH
VLGHRPGNTPVLVGRQVGRKNQSKRFFSLDSIPLEEIDMLSIIIIGNSKTTLVDDIFLTP
HHCCCCCCCCEEEEHHHCCCCCCCEEEEECCCCHHCCCEEEEEEECCCCCEEEEEEEECC
RGYL
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 3B

Specific reaction: S-adenosyl-L-methionine + precorrin-3B = S-adenosyl-L-homocysteine + precorrin-4

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8501034; 11677609 [H]