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The map label for this gene is pinE [H]

Identifier: 123966928

GI number: 123966928

Start: 1494401

End: 1495123

Strand: Direct

Name: pinE [H]

Synonym: P9515_16951

Alternate gene names: 123966928

Gene position: 1494401-1495123 (Clockwise)

Preceding gene: 123966926

Following gene: 123966929

Centisome position: 87.69

GC content: 24.07

Gene sequence:

>723_bases
TTGAATTCTCGCTTATTATCAATATTATTACATTTATTTGTTCTATTAAGTGTTTTATTAAGAGTATTTGATACAGTTAA
AAATATATGTATTAGAACATTGACTTTTAAATTTAAAAGAAAAAGATTTCTTTTATCTGAAAAAAACAAAACTTGTAAAT
CTATTGGATATGCTAGAGCTATAAATAGTGAATTTGATAACTTAGAAGAACAAATAAAACGTTTAAAAAATGAAGGTTGC
AGTTTAATCTTTTCAGAAATTATAAGTTTGGATGAAAAAATAAAACCAGAACTAAAAAAAGCTTTAAATTATTTATCAAA
AGGAGATGAATTAATCCTTACTGAACTAGATCGCGCATTTTCCAATAGAAATGAATGCATAAAAACAATCACTAAACTAT
TGAATAATGAAGTTAGTTTGAGAACTTTATCTGGTTTTTTATCTCCAAAAAATTCTTCAGAGATATATTCTTCAGTTTTT
AATATCTTATATGAATTAGACAAATTAGATAATGAATGCTTAGGAGAAAGAAAAAAAGAAATAATTTTACAAAGAAGATT
AAATGGTAATAATTTAGGCGGCAGACCAAAAATTAGTCCTTTAAAAGAATCATTAGTAATTAGATTACGTAACGAGGGTT
GTTCATATCGATCAATTAGAACTCAAACTGGAATTGCTTTATCAACAATTAGAAGAATAATTTTAGATGGAGAAGATAAT
TAA

Upstream 100 bases:

>100_bases
TTTTATGACAAATAAACCTAGGAAATGTGTTCTGAAATCATATTAAAGAACGCTTTATAGAACGTTATTTTTAATACAGT
TTCCGAACAACTTCAAACTC

Downstream 100 bases:

>100_bases
GATGAATAAAAAAGAAATTGAAAACCTTATTAAAGAAAATCTTAAAGATACAGCATTACGAATTCATGAATTAGATAGTG
AAGATAGAAATAGTTTAATT

Product: site-specific recombinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MNSRLLSILLHLFVLLSVLLRVFDTVKNICIRTLTFKFKRKRFLLSEKNKTCKSIGYARAINSEFDNLEEQIKRLKNEGC
SLIFSEIISLDEKIKPELKKALNYLSKGDELILTELDRAFSNRNECIKTITKLLNNEVSLRTLSGFLSPKNSSEIYSSVF
NILYELDKLDNECLGERKKEIILQRRLNGNNLGGRPKISPLKESLVIRLRNEGCSYRSIRTQTGIALSTIRRIILDGEDN

Sequences:

>Translated_240_residues
MNSRLLSILLHLFVLLSVLLRVFDTVKNICIRTLTFKFKRKRFLLSEKNKTCKSIGYARAINSEFDNLEEQIKRLKNEGC
SLIFSEIISLDEKIKPELKKALNYLSKGDELILTELDRAFSNRNECIKTITKLLNNEVSLRTLSGFLSPKNSSEIYSSVF
NILYELDKLDNECLGERKKEIILQRRLNGNNLGGRPKISPLKESLVIRLRNEGCSYRSIRTQTGIALSTIRRIILDGEDN
>Mature_240_residues
MNSRLLSILLHLFVLLSVLLRVFDTVKNICIRTLTFKFKRKRFLLSEKNKTCKSIGYARAINSEFDNLEEQIKRLKNEGC
SLIFSEIISLDEKIKPELKKALNYLSKGDELILTELDRAFSNRNECIKTITKLLNNEVSLRTLSGFLSPKNSSEIYSSVF
NILYELDKLDNECLGERKKEIILQRRLNGNNLGGRPKISPLKESLVIRLRNEGCSYRSIRTQTGIALSTIRRIILDGEDN

Specific function: This protein catalyzes the inversion of an 1800-bp E.coli DNA fragment, the P region, which can exist in either orientation. The function of the inversion is not yet clear [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=176, Percent_Identity=28.4090909090909, Blast_Score=68, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 27617; Mature: 27617

Theoretical pI: Translated: 10.09; Mature: 10.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSRLLSILLHLFVLLSVLLRVFDTVKNICIRTLTFKFKRKRFLLSEKNKTCKSIGYARA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
INSEFDNLEEQIKRLKNEGCSLIFSEIISLDEKIKPELKKALNYLSKGDELILTELDRAF
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHHHH
SNRNECIKTITKLLNNEVSLRTLSGFLSPKNSSEIYSSVFNILYELDKLDNECLGERKKE
CCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
IILQRRLNGNNLGGRPKISPLKESLVIRLRNEGCSYRSIRTQTGIALSTIRRIILDGEDN
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNSRLLSILLHLFVLLSVLLRVFDTVKNICIRTLTFKFKRKRFLLSEKNKTCKSIGYARA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
INSEFDNLEEQIKRLKNEGCSLIFSEIISLDEKIKPELKKALNYLSKGDELILTELDRAF
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHHHH
SNRNECIKTITKLLNNEVSLRTLSGFLSPKNSSEIYSSVFNILYELDKLDNECLGERKKE
CCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
IILQRRLNGNNLGGRPKISPLKESLVIRLRNEGCSYRSIRTQTGIALSTIRRIILDGEDN
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3894006; 6310572; 8905232; 9278503 [H]