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The map label for this gene is fusA [H]

Identifier: 123966922

GI number: 123966922

Start: 1485123

End: 1487198

Strand: Reverse

Name: fusA [H]

Synonym: P9515_16891

Alternate gene names: 123966922

Gene position: 1487198-1485123 (Counterclockwise)

Preceding gene: 123966923

Following gene: 123966921

Centisome position: 87.27

GC content: 38.92

Gene sequence:

>2076_bases
TTGGCACGCGACTTTCCCCTTGAACGAGTAAGGAATATAGGTATAGCCGCTCATATTGATGCTGGTAAAACAACTACTAC
TGAAAGAATACTTTTTTATTCAGGGGTTGTTCACAAAATCGGAGAAGTACATGATGGTGCTGCAGTAACGGATTGGATGG
ACCAAGAAAGAGAAAGAGGGATTACCATCACAGCTGCTGCAATCTCTACAAGTTGGCAGGATCATAGAATTAATATCATT
GATACTCCAGGTCACGTTGACTTCACAATTGAAGTAGAAAGATCAATGAGAGTTTTAGATGGTGTCATAGCTGTATTTTG
TGCTGTTGGAGGAGTTCAGCCACAATCAGAGACTGTATGGAGACAAGCTGATAGATATTCTGTTCCAAGGATGGTCTTCG
TCAATAAAATGGACAGAACAGGAGCAGACTTTTTAAAAGTTAATAAACAAATTAAAGAGCGCCTCAAAGCAAATGCTTTC
CCTATTCAGCTACCGATTGGTGCTGAGGGTGATCTTACAGGAATTATCGATTTAGTTAGTAACAAGGCTTACTTATACAA
AAATGATTTAGGGACTGATATTGAGGAGGCTCCAATACCAGATGAGATGAAAGATGAGGCATTGGAGTGGAGAAGTAAGC
TAATGGAAAGTGTCGCAGAAAATGACGAAGAATTAATTGAAATATTTTTGGATAAAGGAGAATTAACTGAAGATCAATTA
AAAAAAGGCATTAGAGAAGGAGTTCTTAAGCATGGTTTAGTTCCCGTTTTATGTGGATCAGCCTTTAAAAATAAAGGGGT
TCAATTAGTTCTTGATGCAGTAGTTGATTATTTGCCAGCTCCAGTTGATGTAAAGCCAATTCAAGGGGTTTTACCAAATG
GTAAAGAAGATATCAGACCCTCAGATGATAATGCGCCTTTCAGTGCATTAGCTTTTAAAGTAATGTCAGATCCTTACGGA
AAACTAACTTTTGTAAGAATGTATTCAGGAGTGCTTTCAAAAGGTAGTTATGTTATGAATTCAACTAAGGATGCTAAGGA
GAGAATCTCTAGATTAGTAATCTTAAAAGCTGATGAAAGGGAAGAAGTTGATGAATTAAGAGCAGGTGATTTAGGGGCTG
TTTTAGGCTTGAAAAACACAACAACTGGTGACACATTATGTACTACGGATGATCCAATAGTTCTAGAAACTTTATTTATT
CCTGAACCAGTTATTTCAGTTGCTGTTGAACCTAAAACTAAAGGAGATATGGAAAAGCTCTCAAAAGCTTTACAAGCCTT
ATCAGAGGAAGATCCAACCTTCAGGGTTAGCACAGATCAAGAGACGAATCAGACCGTTATAGCTGGTATGGGTGAATTAC
ATTTGGAAATACTTGTAGATAGAATGTTAAGAGAGTTTAAAGTTGAGGCAAATATCGGTGCTCCTCAAGTCTCGTATAGA
GAAACAATCAGATCTAGTTCTAGGGGTGAAGGTAAGTATGCAAGACAAACAGGAGGTAAGGGGCAGTATGGACACGTAGT
TATTGAAATGGAACCTGCTGAAGTTGGTAAAGGTTTTGAATTTGTTAACAAAATTGTTGGTGGAACAGTTCCAAAAGAAT
ATATTGGACCAGCTTCAAATGGGATGAAAGAAACCTGTGAATCAGGCGTTCTTGCCGGTTATCCGCTGATTGATGTAAAA
GTAACACTAGTCGATGGTTCGTTCCACGATGTAGACTCTTCTGAAATGGCCTTCAAAATTGCAGGTTCCATGGCTTTTAA
AGATGGGGTTAAAAAATGCAATCCTGTCCTATTAGAGCCTATGATGAAAGTTGAGGTCGAAAGTCCTGATGATTTTCTTG
GATCTGTAATTGGTGACCTCTCCTCAAGGAGAGGTCAAGTCGAAGGACAATCTGTCGATGATGGATTGTCTAAAGTACAG
GCCAAAGTGCCTTTAGCCGAAATGTTCGGTTATGCCACTCAACTCCGATCAATGACACAAGGTCGGGGTATATTTTCAAT
GGAGTTCGCAAATTATGAGGAAGTTCCTCGTAATGTTGCTGAAGCTATCATTTCCAAGAATCAGGGCAACTCCTGA

Upstream 100 bases:

>100_bases
CGACCCAAAATGGTCCTTAATTAGTCTATTATTGTAAGAAGTTTATTTGATTAGATAAACTGCAGCTATTTAGCAATTCA
TTCTATTTGGAGTTTTAACA

Downstream 100 bases:

>100_bases
TCTCTAAACTAAACACTCTTTAACCCTCGATTCTTTAATTCAAATGGCTCGCGAGAAGTTCGAAAGGAACAAACCACATG
TCAACATAGGTACTATCGGC

Product: elongation factor G

Products: GDP; phosphate

Alternate protein names: EF-G [H]

Number of amino acids: Translated: 691; Mature: 690

Protein sequence:

>691_residues
MARDFPLERVRNIGIAAHIDAGKTTTTERILFYSGVVHKIGEVHDGAAVTDWMDQERERGITITAAAISTSWQDHRINII
DTPGHVDFTIEVERSMRVLDGVIAVFCAVGGVQPQSETVWRQADRYSVPRMVFVNKMDRTGADFLKVNKQIKERLKANAF
PIQLPIGAEGDLTGIIDLVSNKAYLYKNDLGTDIEEAPIPDEMKDEALEWRSKLMESVAENDEELIEIFLDKGELTEDQL
KKGIREGVLKHGLVPVLCGSAFKNKGVQLVLDAVVDYLPAPVDVKPIQGVLPNGKEDIRPSDDNAPFSALAFKVMSDPYG
KLTFVRMYSGVLSKGSYVMNSTKDAKERISRLVILKADEREEVDELRAGDLGAVLGLKNTTTGDTLCTTDDPIVLETLFI
PEPVISVAVEPKTKGDMEKLSKALQALSEEDPTFRVSTDQETNQTVIAGMGELHLEILVDRMLREFKVEANIGAPQVSYR
ETIRSSSRGEGKYARQTGGKGQYGHVVIEMEPAEVGKGFEFVNKIVGGTVPKEYIGPASNGMKETCESGVLAGYPLIDVK
VTLVDGSFHDVDSSEMAFKIAGSMAFKDGVKKCNPVLLEPMMKVEVESPDDFLGSVIGDLSSRRGQVEGQSVDDGLSKVQ
AKVPLAEMFGYATQLRSMTQGRGIFSMEFANYEEVPRNVAEAIISKNQGNS

Sequences:

>Translated_691_residues
MARDFPLERVRNIGIAAHIDAGKTTTTERILFYSGVVHKIGEVHDGAAVTDWMDQERERGITITAAAISTSWQDHRINII
DTPGHVDFTIEVERSMRVLDGVIAVFCAVGGVQPQSETVWRQADRYSVPRMVFVNKMDRTGADFLKVNKQIKERLKANAF
PIQLPIGAEGDLTGIIDLVSNKAYLYKNDLGTDIEEAPIPDEMKDEALEWRSKLMESVAENDEELIEIFLDKGELTEDQL
KKGIREGVLKHGLVPVLCGSAFKNKGVQLVLDAVVDYLPAPVDVKPIQGVLPNGKEDIRPSDDNAPFSALAFKVMSDPYG
KLTFVRMYSGVLSKGSYVMNSTKDAKERISRLVILKADEREEVDELRAGDLGAVLGLKNTTTGDTLCTTDDPIVLETLFI
PEPVISVAVEPKTKGDMEKLSKALQALSEEDPTFRVSTDQETNQTVIAGMGELHLEILVDRMLREFKVEANIGAPQVSYR
ETIRSSSRGEGKYARQTGGKGQYGHVVIEMEPAEVGKGFEFVNKIVGGTVPKEYIGPASNGMKETCESGVLAGYPLIDVK
VTLVDGSFHDVDSSEMAFKIAGSMAFKDGVKKCNPVLLEPMMKVEVESPDDFLGSVIGDLSSRRGQVEGQSVDDGLSKVQ
AKVPLAEMFGYATQLRSMTQGRGIFSMEFANYEEVPRNVAEAIISKNQGNS
>Mature_690_residues
ARDFPLERVRNIGIAAHIDAGKTTTTERILFYSGVVHKIGEVHDGAAVTDWMDQERERGITITAAAISTSWQDHRINIID
TPGHVDFTIEVERSMRVLDGVIAVFCAVGGVQPQSETVWRQADRYSVPRMVFVNKMDRTGADFLKVNKQIKERLKANAFP
IQLPIGAEGDLTGIIDLVSNKAYLYKNDLGTDIEEAPIPDEMKDEALEWRSKLMESVAENDEELIEIFLDKGELTEDQLK
KGIREGVLKHGLVPVLCGSAFKNKGVQLVLDAVVDYLPAPVDVKPIQGVLPNGKEDIRPSDDNAPFSALAFKVMSDPYGK
LTFVRMYSGVLSKGSYVMNSTKDAKERISRLVILKADEREEVDELRAGDLGAVLGLKNTTTGDTLCTTDDPIVLETLFIP
EPVISVAVEPKTKGDMEKLSKALQALSEEDPTFRVSTDQETNQTVIAGMGELHLEILVDRMLREFKVEANIGAPQVSYRE
TIRSSSRGEGKYARQTGGKGQYGHVVIEMEPAEVGKGFEFVNKIVGGTVPKEYIGPASNGMKETCESGVLAGYPLIDVKV
TLVDGSFHDVDSSEMAFKIAGSMAFKDGVKKCNPVLLEPMMKVEVESPDDFLGSVIGDLSSRRGQVEGQSVDDGLSKVQA
KVPLAEMFGYATQLRSMTQGRGIFSMEFANYEEVPRNVAEAIISKNQGNS

Specific function: Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and

COG id: COG0480

COG function: function code J; Translation elongation factors (GTPases)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=697, Percent_Identity=43.4720229555237, Blast_Score=573, Evalue=1e-163,
Organism=Homo sapiens, GI19923640, Length=728, Percent_Identity=39.5604395604396, Blast_Score=484, Evalue=1e-136,
Organism=Homo sapiens, GI25306287, Length=727, Percent_Identity=37.5515818431912, Blast_Score=444, Evalue=1e-124,
Organism=Homo sapiens, GI25306283, Length=445, Percent_Identity=42.9213483146067, Blast_Score=327, Evalue=3e-89,
Organism=Homo sapiens, GI157426893, Length=157, Percent_Identity=37.5796178343949, Blast_Score=102, Evalue=2e-21,
Organism=Homo sapiens, GI4503483, Length=483, Percent_Identity=23.8095238095238, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI94966754, Length=134, Percent_Identity=38.0597014925373, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI310132016, Length=118, Percent_Identity=38.135593220339, Blast_Score=79, Evalue=2e-14,
Organism=Homo sapiens, GI310110807, Length=118, Percent_Identity=38.135593220339, Blast_Score=79, Evalue=2e-14,
Organism=Homo sapiens, GI310123363, Length=118, Percent_Identity=38.135593220339, Blast_Score=79, Evalue=2e-14,
Organism=Homo sapiens, GI94966752, Length=192, Percent_Identity=26.5625, Blast_Score=67, Evalue=5e-11,
Organism=Escherichia coli, GI1789738, Length=699, Percent_Identity=61.2303290414878, Blast_Score=867, Evalue=0.0,
Organism=Escherichia coli, GI1790835, Length=473, Percent_Identity=28.7526427061311, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI48994988, Length=203, Percent_Identity=37.9310344827586, Blast_Score=120, Evalue=3e-28,
Organism=Escherichia coli, GI1788922, Length=152, Percent_Identity=36.1842105263158, Blast_Score=92, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17533571, Length=680, Percent_Identity=42.6470588235294, Blast_Score=537, Evalue=1e-153,
Organism=Caenorhabditis elegans, GI17556745, Length=692, Percent_Identity=28.1791907514451, Blast_Score=281, Evalue=6e-76,
Organism=Caenorhabditis elegans, GI17506493, Length=824, Percent_Identity=25.2427184466019, Blast_Score=173, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI17557151, Length=155, Percent_Identity=37.4193548387097, Blast_Score=103, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI71988819, Length=134, Percent_Identity=31.3432835820896, Blast_Score=79, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI71988811, Length=134, Percent_Identity=31.3432835820896, Blast_Score=78, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17552882, Length=135, Percent_Identity=34.8148148148148, Blast_Score=73, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6323098, Length=690, Percent_Identity=43.3333333333333, Blast_Score=579, Evalue=1e-166,
Organism=Saccharomyces cerevisiae, GI6322359, Length=797, Percent_Identity=33.7515683814304, Blast_Score=400, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6324707, Length=799, Percent_Identity=24.7809762202753, Blast_Score=167, Evalue=6e-42,
Organism=Saccharomyces cerevisiae, GI6320593, Length=799, Percent_Identity=24.7809762202753, Blast_Score=167, Evalue=6e-42,
Organism=Saccharomyces cerevisiae, GI6323320, Length=201, Percent_Identity=34.8258706467662, Blast_Score=93, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6324166, Length=144, Percent_Identity=32.6388888888889, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24582462, Length=694, Percent_Identity=44.0922190201729, Blast_Score=599, Evalue=1e-171,
Organism=Drosophila melanogaster, GI221458488, Length=725, Percent_Identity=32.4137931034483, Blast_Score=360, Evalue=3e-99,
Organism=Drosophila melanogaster, GI24585711, Length=487, Percent_Identity=23.6139630390144, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24585713, Length=487, Percent_Identity=23.6139630390144, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24585709, Length=487, Percent_Identity=23.6139630390144, Blast_Score=102, Evalue=7e-22,
Organism=Drosophila melanogaster, GI78706572, Length=160, Percent_Identity=37.5, Blast_Score=99, Evalue=8e-21,
Organism=Drosophila melanogaster, GI28574573, Length=141, Percent_Identity=36.8794326241135, Blast_Score=87, Evalue=4e-17,
Organism=Drosophila melanogaster, GI21357743, Length=135, Percent_Identity=31.8518518518519, Blast_Score=70, Evalue=4e-12,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 7984 Molecules/Cell In: Growth Phase, Gl

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR000795
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR005225
- InterPro:   IPR004540
- InterPro:   IPR000640
- InterPro:   IPR005517
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF03764 EFG_IV; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 75778; Mature: 75647

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARDFPLERVRNIGIAAHIDAGKTTTTERILFYSGVVHKIGEVHDGAAVTDWMDQERERG
CCCCCCHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC
ITITAAAISTSWQDHRINIIDTPGHVDFTIEVERSMRVLDGVIAVFCAVGGVQPQSETVW
EEEEEEEEECCCCCCEEEEEECCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
RQADRYSVPRMVFVNKMDRTGADFLKVNKQIKERLKANAFPIQLPIGAEGDLTGIIDLVS
HHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHC
NKAYLYKNDLGTDIEEAPIPDEMKDEALEWRSKLMESVAENDEELIEIFLDKGELTEDQL
CCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHH
KKGIREGVLKHGLVPVLCGSAFKNKGVQLVLDAVVDYLPAPVDVKPIQGVLPNGKEDIRP
HHHHHHHHHHHCCHHHHHCCHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
SDDNAPFSALAFKVMSDPYGKLTFVRMYSGVLSKGSYVMNSTKDAKERISRLVILKADER
CCCCCCHHHHHHHHHCCCCCCEEHHHHHHHHHCCCCEEECCCHHHHHHHHEEEEEECCCH
EEVDELRAGDLGAVLGLKNTTTGDTLCTTDDPIVLETLFIPEPVISVAVEPKTKGDMEKL
HHHHHHCCCCCCEEEECCCCCCCCEEECCCCCEEEEEEECCCCHHEEEECCCCCCCHHHH
SKALQALSEEDPTFRVSTDQETNQTVIAGMGELHLEILVDRMLREFKVEANIGAPQVSYR
HHHHHHHCCCCCCEEEECCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
ETIRSSSRGEGKYARQTGGKGQYGHVVIEMEPAEVGKGFEFVNKIVGGTVPKEYIGPASN
HHHHCCCCCCCCHHHHCCCCCCCCEEEEEECHHHHCCHHHHHHHHHCCCCCHHHCCCCCC
GMKETCESGVLAGYPLIDVKVTLVDGSFHDVDSSEMAFKIAGSMAFKDGVKKCNPVLLEP
CHHHHHHCCCCCCCCEEEEEEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHCCHHHHCC
MMKVEVESPDDFLGSVIGDLSSRRGQVEGQSVDDGLSKVQAKVPLAEMFGYATQLRSMTQ
HHEEEECCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
GRGIFSMEFANYEEVPRNVAEAIISKNQGNS
CCCEEEEECCCHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
ARDFPLERVRNIGIAAHIDAGKTTTTERILFYSGVVHKIGEVHDGAAVTDWMDQERERG
CCCCCHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC
ITITAAAISTSWQDHRINIIDTPGHVDFTIEVERSMRVLDGVIAVFCAVGGVQPQSETVW
EEEEEEEEECCCCCCEEEEEECCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
RQADRYSVPRMVFVNKMDRTGADFLKVNKQIKERLKANAFPIQLPIGAEGDLTGIIDLVS
HHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHC
NKAYLYKNDLGTDIEEAPIPDEMKDEALEWRSKLMESVAENDEELIEIFLDKGELTEDQL
CCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHH
KKGIREGVLKHGLVPVLCGSAFKNKGVQLVLDAVVDYLPAPVDVKPIQGVLPNGKEDIRP
HHHHHHHHHHHCCHHHHHCCHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
SDDNAPFSALAFKVMSDPYGKLTFVRMYSGVLSKGSYVMNSTKDAKERISRLVILKADER
CCCCCCHHHHHHHHHCCCCCCEEHHHHHHHHHCCCCEEECCCHHHHHHHHEEEEEECCCH
EEVDELRAGDLGAVLGLKNTTTGDTLCTTDDPIVLETLFIPEPVISVAVEPKTKGDMEKL
HHHHHHCCCCCCEEEECCCCCCCCEEECCCCCEEEEEEECCCCHHEEEECCCCCCCHHHH
SKALQALSEEDPTFRVSTDQETNQTVIAGMGELHLEILVDRMLREFKVEANIGAPQVSYR
HHHHHHHCCCCCCEEEECCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
ETIRSSSRGEGKYARQTGGKGQYGHVVIEMEPAEVGKGFEFVNKIVGGTVPKEYIGPASN
HHHHCCCCCCCCHHHHCCCCCCCCEEEEEECHHHHCCHHHHHHHHHCCCCCHHHCCCCCC
GMKETCESGVLAGYPLIDVKVTLVDGSFHDVDSSEMAFKIAGSMAFKDGVKKCNPVLLEP
CHHHHHHCCCCCCCCEEEEEEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHCCHHHHCC
MMKVEVESPDDFLGSVIGDLSSRRGQVEGQSVDDGLSKVQAKVPLAEMFGYATQLRSMTQ
HHEEEECCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
GRGIFSMEFANYEEVPRNVAEAIISKNQGNS
CCCEEEEECCCHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA