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The map label for this gene is purB [H]

Identifier: 123966878

GI number: 123966878

Start: 1435108

End: 1436403

Strand: Direct

Name: purB [H]

Synonym: P9515_16451

Alternate gene names: 123966878

Gene position: 1435108-1436403 (Clockwise)

Preceding gene: 123966877

Following gene: 123966879

Centisome position: 84.21

GC content: 34.1

Gene sequence:

>1296_bases
GTGATTGAACGTTACACATTGCCCGAAATGGGGAGAATCTGGACTGAACATGCGAAATTCCAGAGTTGGCTTAAAGTTGA
AATTGCAGCATGCGAAGCAAATTATTCTCTTGGGAAAATTCCTGAAGATGCACTTCAAGAGATTCGTTTAAAGGCTAAAT
TTGAAGAATCAAGAATTAAAGAAATTGAGAAAGAGGTTAAACATGATGTAATAGCATTTCTTACAAATATTAATGAATAT
GTTGGAGATTCCGGAAGATATATTCATGTTGGAATGACTAGCAGTGATGTCCTTGATACAGGCCTATCATTACAGCTAAA
AGATTCCTGCAAATTGTTATTAGAAGAGATTGAAAAATTAGAAAATGGAGTCAGGTTATTAGCGAGAAAGCATAAAAATA
CATTAATGATTGGAAGATCGCATGCCATTCACGGAGAGCCTATCTCTTTTGGATTTAAATTGGCGGGATGGTTAGCGGAA
ATATTAAGAGATAAGAAACGATTATTAAACCTTAAAGAATCTATTTCCATAGGACAAATAAGTGGAGCCATGGGAACTTA
CGCTAACACAAACCCCGAAATAGAAAAAATAACTTGCGATTTACTAGATTTAAAACCAGATACTGCAAGTACTCAGGTTA
TATCAAGAGATAGACATGCTGAATATGTTCAGACAATTGCTTTAGTAGGTGCTTCACTTGACAGATTTGCGACTGAAATA
AGGAACTTGCAGAGAACAGATGTGTTAGAAGTTGAAGAAGGATTTTCAAAAGGGCAAAAAGGAAGCTCAGCCATGCCACA
TAAAAGAAACCCAATTCGCAGTGAAAGGGTTAGTGGTTTATCTAGAATTTTGAGAAGTTATGTTGTTACAGCTCTTGAGA
ATGTTCCTCTTTGGCACGAAAGAGATATTAGCCATAGTTCTAATGAGCGCATCATGTTACCAGATGTTTCTATATGTCTT
CATTTTATGCTTAGGGAAATGCAGGAAATAATAAATAATTTAGAAGTTTATCCAGAAAATATGTTTAAAAATTTAAATAT
ATACGGGGGGGTAATCTTTAGCCAGAAAGTTCTACTTTTACTTGTAGAAAAAGGTTTGTCTAGGGAAAAGGCTTATAGTT
TGGTACAGAAAAATGCTCACCAAGCTTGGAATAATGAGAATGGCAATTTCAAAAAGAATATCGAGAAAGATCAAGAGATA
ATGAAATTAGTAACTGAGAATGATTTAAAGGAATGCTTCGACCCCAACATTCATCTAAATAATTTAAATGTAATATGGGA
AAAATTAAGTATTTAA

Upstream 100 bases:

>100_bases
AATTCTTATGCAAATATGTTCAATGGCAAGTAGATCTTTAAGGTATAGAGCAATTATGAGATTTTAGTTATAGAGTTGAA
ATTGAAGATGTTTAAAAATT

Downstream 100 bases:

>100_bases
ATCACCTAAATATTATTTGAATTTTAACCACAGTTTTTTTTACAACTAATGACCAAAAACTTTAGATTTGAAAAAGATAG
TATGGGAAAAATTAAAGTTC

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MIERYTLPEMGRIWTEHAKFQSWLKVEIAACEANYSLGKIPEDALQEIRLKAKFEESRIKEIEKEVKHDVIAFLTNINEY
VGDSGRYIHVGMTSSDVLDTGLSLQLKDSCKLLLEEIEKLENGVRLLARKHKNTLMIGRSHAIHGEPISFGFKLAGWLAE
ILRDKKRLLNLKESISIGQISGAMGTYANTNPEIEKITCDLLDLKPDTASTQVISRDRHAEYVQTIALVGASLDRFATEI
RNLQRTDVLEVEEGFSKGQKGSSAMPHKRNPIRSERVSGLSRILRSYVVTALENVPLWHERDISHSSNERIMLPDVSICL
HFMLREMQEIINNLEVYPENMFKNLNIYGGVIFSQKVLLLLVEKGLSREKAYSLVQKNAHQAWNNENGNFKKNIEKDQEI
MKLVTENDLKECFDPNIHLNNLNVIWEKLSI

Sequences:

>Translated_431_residues
MIERYTLPEMGRIWTEHAKFQSWLKVEIAACEANYSLGKIPEDALQEIRLKAKFEESRIKEIEKEVKHDVIAFLTNINEY
VGDSGRYIHVGMTSSDVLDTGLSLQLKDSCKLLLEEIEKLENGVRLLARKHKNTLMIGRSHAIHGEPISFGFKLAGWLAE
ILRDKKRLLNLKESISIGQISGAMGTYANTNPEIEKITCDLLDLKPDTASTQVISRDRHAEYVQTIALVGASLDRFATEI
RNLQRTDVLEVEEGFSKGQKGSSAMPHKRNPIRSERVSGLSRILRSYVVTALENVPLWHERDISHSSNERIMLPDVSICL
HFMLREMQEIINNLEVYPENMFKNLNIYGGVIFSQKVLLLLVEKGLSREKAYSLVQKNAHQAWNNENGNFKKNIEKDQEI
MKLVTENDLKECFDPNIHLNNLNVIWEKLSI
>Mature_431_residues
MIERYTLPEMGRIWTEHAKFQSWLKVEIAACEANYSLGKIPEDALQEIRLKAKFEESRIKEIEKEVKHDVIAFLTNINEY
VGDSGRYIHVGMTSSDVLDTGLSLQLKDSCKLLLEEIEKLENGVRLLARKHKNTLMIGRSHAIHGEPISFGFKLAGWLAE
ILRDKKRLLNLKESISIGQISGAMGTYANTNPEIEKITCDLLDLKPDTASTQVISRDRHAEYVQTIALVGASLDRFATEI
RNLQRTDVLEVEEGFSKGQKGSSAMPHKRNPIRSERVSGLSRILRSYVVTALENVPLWHERDISHSSNERIMLPDVSICL
HFMLREMQEIINNLEVYPENMFKNLNIYGGVIFSQKVLLLLVEKGLSREKAYSLVQKNAHQAWNNENGNFKKNIEKDQEI
MKLVTENDLKECFDPNIHLNNLNVIWEKLSI

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=398, Percent_Identity=26.8844221105528, Blast_Score=149, Evalue=7e-36,
Organism=Homo sapiens, GI183227688, Length=385, Percent_Identity=27.2727272727273, Blast_Score=146, Evalue=4e-35,
Organism=Escherichia coli, GI1787376, Length=373, Percent_Identity=26.0053619302949, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790398, Length=251, Percent_Identity=24.3027888446215, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17508577, Length=388, Percent_Identity=27.8350515463918, Blast_Score=140, Evalue=9e-34,
Organism=Caenorhabditis elegans, GI32564234, Length=334, Percent_Identity=28.1437125748503, Blast_Score=117, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6323391, Length=396, Percent_Identity=25.5050505050505, Blast_Score=139, Evalue=6e-34,
Organism=Drosophila melanogaster, GI24647570, Length=382, Percent_Identity=25.9162303664921, Blast_Score=126, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 49294; Mature: 49294

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIERYTLPEMGRIWTEHAKFQSWLKVEIAACEANYSLGKIPEDALQEIRLKAKFEESRIK
CCCCCCCCHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHH
EIEKEVKHDVIAFLTNINEYVGDSGRYIHVGMTSSDVLDTGLSLQLKDSCKLLLEEIEKL
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHCCCCEEEHHHHHHHHHHHHHH
ENGVRLLARKHKNTLMIGRSHAIHGEPISFGFKLAGWLAEILRDKKRLLNLKESISIGQI
HHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
SGAMGTYANTNPEIEKITCDLLDLKPDTASTQVISRDRHAEYVQTIALVGASLDRFATEI
CCCCCCCCCCCCCHHHEEEEHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RNLQRTDVLEVEEGFSKGQKGSSAMPHKRNPIRSERVSGLSRILRSYVVTALENVPLWHE
HHCHHCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
RDISHSSNERIMLPDVSICLHFMLREMQEIINNLEVYPENMFKNLNIYGGVIFSQKVLLL
CCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHH
LVEKGLSREKAYSLVQKNAHQAWNNENGNFKKNIEKDQEIMKLVTENDLKECFDPNIHLN
HHHHCCCHHHHHHHHHHHHHHHCCCCCCCCHHCHHHHHHHHHHHHHHHHHHHCCCCEEEC
NLNVIWEKLSI
CHHHHHHHHCC
>Mature Secondary Structure
MIERYTLPEMGRIWTEHAKFQSWLKVEIAACEANYSLGKIPEDALQEIRLKAKFEESRIK
CCCCCCCCHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHH
EIEKEVKHDVIAFLTNINEYVGDSGRYIHVGMTSSDVLDTGLSLQLKDSCKLLLEEIEKL
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHCCCCEEEHHHHHHHHHHHHHH
ENGVRLLARKHKNTLMIGRSHAIHGEPISFGFKLAGWLAEILRDKKRLLNLKESISIGQI
HHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
SGAMGTYANTNPEIEKITCDLLDLKPDTASTQVISRDRHAEYVQTIALVGASLDRFATEI
CCCCCCCCCCCCCHHHEEEEHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RNLQRTDVLEVEEGFSKGQKGSSAMPHKRNPIRSERVSGLSRILRSYVVTALENVPLWHE
HHCHHCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
RDISHSSNERIMLPDVSICLHFMLREMQEIINNLEVYPENMFKNLNIYGGVIFSQKVLLL
CCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHH
LVEKGLSREKAYSLVQKNAHQAWNNENGNFKKNIEKDQEIMKLVTENDLKECFDPNIHLN
HHHHCCCHHHHHHHHHHHHHHHCCCCCCCCHHCHHHHHHHHHHHHHHHHHHHCCCCEEEC
NLNVIWEKLSI
CHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]