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The map label for this gene is nadE [H]

Identifier: 123966858

GI number: 123966858

Start: 1420214

End: 1421911

Strand: Direct

Name: nadE [H]

Synonym: P9515_16251

Alternate gene names: 123966858

Gene position: 1420214-1421911 (Clockwise)

Preceding gene: 123966857

Following gene: 123966870

Centisome position: 83.34

GC content: 30.62

Gene sequence:

>1698_bases
ATGAAGTTTTTCCTTGCTCAATTAAATCCAATTGTGGGCGATCTAGATGGAAATGCAAAAAAAATACTCTATGTAGCTAA
TAAAGCACATTTAAATTCTGCTGATATGGTGCTCACCCCAGAATTATCTTTGTGGGGTTATCCTGCAAAAGATTTACTTT
TAAAAAAAAATTTAATTGAAAGACAATATACTATTCTAGATCAGCTATCCAAATCTATTAATAAAAAATATGGGGATTTG
AGCATTACGGTAGGGATTGCTGAAAAGATTGATGACTCTTTTTTCCCAAATCTTTATAATTCTGTTGTTCTAATTGAGAG
TGGTCATTGGAAAACAATTGCACGAAAAATTATTCTCCCTACTTATGAAGTATTTGATGAGAAAAGATACTTTCGATCCG
AAGAGAAAGTATCTGTAATCTCCAAGAAAATTAAAGATAAAACTTATAAGATTGGCTTAACTATATGTGAAGATTTATGG
GTTAATGAAAATATAGATGGGAGAGGTATTCATGAAAAGAATCCAATTTCGCAATTAAAGAGTAAAAAAATTGATCTTTT
ATTAAATTTATCGGCATCTCCATACACCTTCAAAAAATTTAAGTTAAGAAATAAAATTTCAAGTTTTGCGGCTCAATATC
TTAATGTGCCTTTGGTATACATCAATCAAGTAGGGGCTAATGATGATCTAATTTTTGATGGGAATAGTTTCATTATCAAT
AAAAATGGATCAAAAATTAAGCAATTAAAATCTTTTTCAGAAGATACATTCATTTGGAATATTAATGAAAATTTAAATTT
GGTAACTGAAAAAAAATCTTCTGATATTTCTTCAGTTTTTGATGCTTTAGTTTTGGGAGTAAAAGATTACGCTAAAAAAT
GCGGATTTAGATCAGCTTTGATTGGCTTAAGTGGAGGAATTGATTCGGCACTCGTTTCAGTAATTGCAACTGCTGCATTA
GGACATGAGAATATCTTCTGCGTCTCAATGCCTTCTAAATGGAGTTCAAATCATTCTAAAATCGATGCAAAAGATTTAGT
CGAAAGATTAAATGTAAATTTAAATACAATTTCAATTGAAAATATCATGTCTTCTTTTGAGGACTCTTTTTTAGAAAGCT
TTAATTTCAAAGTAGAAGGCGTAACAAATCAAAACATCCAATCGAGGATAAGGGGTACCCTTCTAATGGCTTTAGCTAAT
CAAGAAAAGCATTTATTACTTGCAACTGGTAATAAATCAGAACTAGCTGTTGGATATTGCACACTATATGGCGATATGAA
TGGAGGATTATCTGTAATAGGTGATTTATATAAAACTAATGTTTTTAAATTGTGTAATTGGCTAGATAGTGAAGATTCAT
TAGAACGCCGAAAAGCTTACAAATTAGATACTAAAGTTAAAATTATTGGGGACCAAATCCGAAAGAAGGCTCCCAGTGCT
GAGTTAGGACCTAATCAACTTGATACCGATTCACTGCCTCCATATTCTTTATTAGATAAAATTCTTAAAGGAATTATTGA
GGAAAAAAAAGATTTACAGCTTCTTGAACAAGATGGTCATACCAAAAAACAAATTTTAAAAATAATTACACTAATTAAAA
AAGCCGAATTCAAAAGAAAACAAGCTCCTCCTATACTAAAACTAAGTAGTCAATCACTGGGAAGTGACTGGAGAGTCCCA
ATTGCAATTTCTTACTAA

Upstream 100 bases:

>100_bases
AGTTCAACGGTTAGATTAAATAACAATTATTCTGACCTACCAAAAGCTTTAATTGATATAGTGAAGAAGAATAATTTATA
TGAATTTGTTACTTAGTTGA

Downstream 100 bases:

>100_bases
GATCCCATCTTTTTAAAAGTCATTTCTTGAATTTTATTTAAAGGTCTTTTAATTGATTTAGGGTTAATTCCTTCTCTTAT
AGCAAGAACTAAACCTGATG

Product: carbon-nitrogen hydrolase:NAD+ synthase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 565; Mature: 565

Protein sequence:

>565_residues
MKFFLAQLNPIVGDLDGNAKKILYVANKAHLNSADMVLTPELSLWGYPAKDLLLKKNLIERQYTILDQLSKSINKKYGDL
SITVGIAEKIDDSFFPNLYNSVVLIESGHWKTIARKIILPTYEVFDEKRYFRSEEKVSVISKKIKDKTYKIGLTICEDLW
VNENIDGRGIHEKNPISQLKSKKIDLLLNLSASPYTFKKFKLRNKISSFAAQYLNVPLVYINQVGANDDLIFDGNSFIIN
KNGSKIKQLKSFSEDTFIWNINENLNLVTEKKSSDISSVFDALVLGVKDYAKKCGFRSALIGLSGGIDSALVSVIATAAL
GHENIFCVSMPSKWSSNHSKIDAKDLVERLNVNLNTISIENIMSSFEDSFLESFNFKVEGVTNQNIQSRIRGTLLMALAN
QEKHLLLATGNKSELAVGYCTLYGDMNGGLSVIGDLYKTNVFKLCNWLDSEDSLERRKAYKLDTKVKIIGDQIRKKAPSA
ELGPNQLDTDSLPPYSLLDKILKGIIEEKKDLQLLEQDGHTKKQILKIITLIKKAEFKRKQAPPILKLSSQSLGSDWRVP
IAISY

Sequences:

>Translated_565_residues
MKFFLAQLNPIVGDLDGNAKKILYVANKAHLNSADMVLTPELSLWGYPAKDLLLKKNLIERQYTILDQLSKSINKKYGDL
SITVGIAEKIDDSFFPNLYNSVVLIESGHWKTIARKIILPTYEVFDEKRYFRSEEKVSVISKKIKDKTYKIGLTICEDLW
VNENIDGRGIHEKNPISQLKSKKIDLLLNLSASPYTFKKFKLRNKISSFAAQYLNVPLVYINQVGANDDLIFDGNSFIIN
KNGSKIKQLKSFSEDTFIWNINENLNLVTEKKSSDISSVFDALVLGVKDYAKKCGFRSALIGLSGGIDSALVSVIATAAL
GHENIFCVSMPSKWSSNHSKIDAKDLVERLNVNLNTISIENIMSSFEDSFLESFNFKVEGVTNQNIQSRIRGTLLMALAN
QEKHLLLATGNKSELAVGYCTLYGDMNGGLSVIGDLYKTNVFKLCNWLDSEDSLERRKAYKLDTKVKIIGDQIRKKAPSA
ELGPNQLDTDSLPPYSLLDKILKGIIEEKKDLQLLEQDGHTKKQILKIITLIKKAEFKRKQAPPILKLSSQSLGSDWRVP
IAISY
>Mature_565_residues
MKFFLAQLNPIVGDLDGNAKKILYVANKAHLNSADMVLTPELSLWGYPAKDLLLKKNLIERQYTILDQLSKSINKKYGDL
SITVGIAEKIDDSFFPNLYNSVVLIESGHWKTIARKIILPTYEVFDEKRYFRSEEKVSVISKKIKDKTYKIGLTICEDLW
VNENIDGRGIHEKNPISQLKSKKIDLLLNLSASPYTFKKFKLRNKISSFAAQYLNVPLVYINQVGANDDLIFDGNSFIIN
KNGSKIKQLKSFSEDTFIWNINENLNLVTEKKSSDISSVFDALVLGVKDYAKKCGFRSALIGLSGGIDSALVSVIATAAL
GHENIFCVSMPSKWSSNHSKIDAKDLVERLNVNLNTISIENIMSSFEDSFLESFNFKVEGVTNQNIQSRIRGTLLMALAN
QEKHLLLATGNKSELAVGYCTLYGDMNGGLSVIGDLYKTNVFKLCNWLDSEDSLERRKAYKLDTKVKIIGDQIRKKAPSA
ELGPNQLDTDSLPPYSLLDKILKGIIEEKKDLQLLEQDGHTKKQILKIITLIKKAEFKRKQAPPILKLSSQSLGSDWRVP
IAISY

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 63479; Mature: 63479

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFFLAQLNPIVGDLDGNAKKILYVANKAHLNSADMVLTPELSLWGYPAKDLLLKKNLIE
CCEEHHHCCCCEECCCCCCEEEEEEECCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHH
RQYTILDQLSKSINKKYGDLSITVGIAEKIDDSFFPNLYNSVVLIESGHWKTIARKIILP
HHHHHHHHHHHHHHCCCCCEEEEEEEHHHCCCHHCCHHHCCEEEEECCCHHHHHHHHHCC
TYEVFDEKRYFRSEEKVSVISKKIKDKTYKIGLTICEDLWVNENIDGRGIHEKNPISQLK
CHHHHHHHHHHCCHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCHHHHH
SKKIDLLLNLSASPYTFKKFKLRNKISSFAAQYLNVPLVYINQVGANDDLIFDGNSFIIN
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEECCCEEEEE
KNGSKIKQLKSFSEDTFIWNINENLNLVTEKKSSDISSVFDALVLGVKDYAKKCGFRSAL
CCCHHHHHHHCCCCCCEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHH
IGLSGGIDSALVSVIATAALGHENIFCVSMPSKWSSNHSKIDAKDLVERLNVNLNTISIE
HCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHCCCCEEEEHH
NIMSSFEDSFLESFNFKVEGVTNQNIQSRIRGTLLMALANQEKHLLLATGNKSELAVGYC
HHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHEEEECCCCCEEEEEECCCCCEEEEEE
TLYGDMNGGLSVIGDLYKTNVFKLCNWLDSEDSLERRKAYKLDTKVKIIGDQIRKKAPSA
EEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEECHHHHHCCCCC
ELGPNQLDTDSLPPYSLLDKILKGIIEEKKDLQLLEQDGHTKKQILKIITLIKKAEFKRK
CCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
QAPPILKLSSQSLGSDWRVPIAISY
CCCCEEEECCCCCCCCCCEEEEEEC
>Mature Secondary Structure
MKFFLAQLNPIVGDLDGNAKKILYVANKAHLNSADMVLTPELSLWGYPAKDLLLKKNLIE
CCEEHHHCCCCEECCCCCCEEEEEEECCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHH
RQYTILDQLSKSINKKYGDLSITVGIAEKIDDSFFPNLYNSVVLIESGHWKTIARKIILP
HHHHHHHHHHHHHHCCCCCEEEEEEEHHHCCCHHCCHHHCCEEEEECCCHHHHHHHHHCC
TYEVFDEKRYFRSEEKVSVISKKIKDKTYKIGLTICEDLWVNENIDGRGIHEKNPISQLK
CHHHHHHHHHHCCHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCHHHHH
SKKIDLLLNLSASPYTFKKFKLRNKISSFAAQYLNVPLVYINQVGANDDLIFDGNSFIIN
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEECCCEEEEE
KNGSKIKQLKSFSEDTFIWNINENLNLVTEKKSSDISSVFDALVLGVKDYAKKCGFRSAL
CCCHHHHHHHCCCCCCEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHH
IGLSGGIDSALVSVIATAALGHENIFCVSMPSKWSSNHSKIDAKDLVERLNVNLNTISIE
HCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHCCCCEEEEHH
NIMSSFEDSFLESFNFKVEGVTNQNIQSRIRGTLLMALANQEKHLLLATGNKSELAVGYC
HHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHEEEECCCCCEEEEEECCCCCEEEEEE
TLYGDMNGGLSVIGDLYKTNVFKLCNWLDSEDSLERRKAYKLDTKVKIIGDQIRKKAPSA
EEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEECHHHHHCCCCC
ELGPNQLDTDSLPPYSLLDKILKGIIEEKKDLQLLEQDGHTKKQILKIITLIKKAEFKRK
CCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
QAPPILKLSSQSLGSDWRVPIAISY
CCCCEEEECCCCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]