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The map label for this gene is dnaK [H]

Identifier: 123966843

GI number: 123966843

Start: 1406861

End: 1408426

Strand: Direct

Name: dnaK [H]

Synonym: P9515_16101

Alternate gene names: 123966843

Gene position: 1406861-1408426 (Clockwise)

Preceding gene: 123966839

Following gene: 123966845

Centisome position: 82.55

GC content: 31.23

Gene sequence:

>1566_bases
ATGGAAAAGAATTTTTCAGGAACTCTTGCTCTTGATTTGGGAAATACTAATACCGTAATTGCTTTTCAAGATGAAAAAGG
TAAAGATTTAAATTTAATAGAAATACCAGGCATTACATCTTCTCCTGGAGTCATCCCAACAGTCATTTGGTTCGAAGGTG
AAGACAATATCGCTAAGATAGGATTATCTGCATTGAAAAAGAAAAATTTATCTAATTCAGAAACATATTTTCATTCAAAT
TTCAAAAGATTAATTGCTAATCCTGTTGAGAGGCTTCAAAAGAAAGTTTTAAGTCCTAATGAATCCGGTGAAAAATTTTT
CAAAATTCTTTGGGAAAATATTCCAAATGAACTAGAAATCAAAAGGCTCGTCTTGACTGCACCAATTGACACCTATAAAG
GTTATAGAAAGTGGTTAATAAATCTCTGCGAAAGTTTACCTATTAATGAGATTGCACTAGTTGATGAACCTACTGCTGCG
GGAATAGGCATCAATGTTCCATTGGGTTCAATCATTATGATTATCGATATTGGGGGAAGTACAATCGATATGAGTGTAAT
TAAAACACAAGGTGGCGAAGGTAAATCTGCCCCTATTGCAGAACTATTGAAGTTTCAAGGAAAGGATGTGAGTTCAATAT
CAAAGCAAAAAATAAGATGTGCAGAAACAATAAGCAAATCGGGATCAAAAATTGGAGGTAAAGATATAGATCAATGGATT
GTTAATTATTTTTTACCATCAAATCAAGATGAAAGAAATCTTTCAATAGCAGAAAAATTAAAATGTAAGCTTAGTAGTCC
AGGAGTCGAATCTGAAACAAGGCACCTTATCTCATTATTTACAGAAGAAAATGAACAAAAGGAATTTTTTTTGAGTAAAG
AAATTTTTGAAAAGATTTTGATAGATAACAATCTTTTAAATCACTTAAATTCTTTACTTAAAGATTTATTAAATGAAGCT
AGAGGCAAATTTTGTACTATAGATGATTTGAACTCTGTGATTTTGGTTGGAGGAGGAACACAAATTCCTTTAATCAAAGA
GTGGATAGCTAATGAGATTTCAGGGATACAAATCAGAACTCCTCCTCCTATTGAATCAATAGCAGTTGGAGCTCTTGCTA
TGACTCCCGGAGTTAAGATTAAAGATATTCTAATTAAAGGTATATCTATAAGATTATTTAATAAAAGAGAGCAAAAACAA
TTCTGGCATCCGATTTTTTATAAGGGCCAAACATGGCCTACAGAAAAACCATTTGAGTTAATTCTTCAAGCCAGAAAAGA
TGGTCAGAGCATTTTCGAGATCATTATTGGAGAAACTAAAGAGAAAAGAAATTATGATGTGATTTTTGAGAATGGTTTAC
CCAAGTTATCAGAGTATCAAAATGAAGAGGAGGTTCTTAAATGGAATAAAAAGCCCTTAAAAATAAAATTAAACAATGAG
TGTAAAATTGGAGAAGATTGCTTAAAGCTCTATTTCAGTATTACGAAGGATTCATCTCTTTATGTAAAATATCTAGATAT
TAATGAAAAAGAATTAGGCGAATCTAATTTAGGAAATATCTTTTAA

Upstream 100 bases:

>100_bases
AAAATTTTTATTTTAATTATACAATAAAGTTTACATCAACATTAAAGCCATTAAATTTATAAATTTCCAAACAAACTTAA
TAACATAAAAGTAATTTGAT

Downstream 100 bases:

>100_bases
ACATTAACGATCCAAGAAGATACCCTCTTCCCCGTCCACTTGCTTTAAACATAAATGAACAGTTTCTTTTTTGTTAGTAG
GAACTTTTCTTCCACAAAAA

Product: HSP70 family molecular chaperone

Products: NA

Alternate protein names: HSP70; Heat shock 70 kDa protein; Heat shock protein 70 [H]

Number of amino acids: Translated: 521; Mature: 521

Protein sequence:

>521_residues
MEKNFSGTLALDLGNTNTVIAFQDEKGKDLNLIEIPGITSSPGVIPTVIWFEGEDNIAKIGLSALKKKNLSNSETYFHSN
FKRLIANPVERLQKKVLSPNESGEKFFKILWENIPNELEIKRLVLTAPIDTYKGYRKWLINLCESLPINEIALVDEPTAA
GIGINVPLGSIIMIIDIGGSTIDMSVIKTQGGEGKSAPIAELLKFQGKDVSSISKQKIRCAETISKSGSKIGGKDIDQWI
VNYFLPSNQDERNLSIAEKLKCKLSSPGVESETRHLISLFTEENEQKEFFLSKEIFEKILIDNNLLNHLNSLLKDLLNEA
RGKFCTIDDLNSVILVGGGTQIPLIKEWIANEISGIQIRTPPPIESIAVGALAMTPGVKIKDILIKGISIRLFNKREQKQ
FWHPIFYKGQTWPTEKPFELILQARKDGQSIFEIIIGETKEKRNYDVIFENGLPKLSEYQNEEEVLKWNKKPLKIKLNNE
CKIGEDCLKLYFSITKDSSLYVKYLDINEKELGESNLGNIF

Sequences:

>Translated_521_residues
MEKNFSGTLALDLGNTNTVIAFQDEKGKDLNLIEIPGITSSPGVIPTVIWFEGEDNIAKIGLSALKKKNLSNSETYFHSN
FKRLIANPVERLQKKVLSPNESGEKFFKILWENIPNELEIKRLVLTAPIDTYKGYRKWLINLCESLPINEIALVDEPTAA
GIGINVPLGSIIMIIDIGGSTIDMSVIKTQGGEGKSAPIAELLKFQGKDVSSISKQKIRCAETISKSGSKIGGKDIDQWI
VNYFLPSNQDERNLSIAEKLKCKLSSPGVESETRHLISLFTEENEQKEFFLSKEIFEKILIDNNLLNHLNSLLKDLLNEA
RGKFCTIDDLNSVILVGGGTQIPLIKEWIANEISGIQIRTPPPIESIAVGALAMTPGVKIKDILIKGISIRLFNKREQKQ
FWHPIFYKGQTWPTEKPFELILQARKDGQSIFEIIIGETKEKRNYDVIFENGLPKLSEYQNEEEVLKWNKKPLKIKLNNE
CKIGEDCLKLYFSITKDSSLYVKYLDINEKELGESNLGNIF
>Mature_521_residues
MEKNFSGTLALDLGNTNTVIAFQDEKGKDLNLIEIPGITSSPGVIPTVIWFEGEDNIAKIGLSALKKKNLSNSETYFHSN
FKRLIANPVERLQKKVLSPNESGEKFFKILWENIPNELEIKRLVLTAPIDTYKGYRKWLINLCESLPINEIALVDEPTAA
GIGINVPLGSIIMIIDIGGSTIDMSVIKTQGGEGKSAPIAELLKFQGKDVSSISKQKIRCAETISKSGSKIGGKDIDQWI
VNYFLPSNQDERNLSIAEKLKCKLSSPGVESETRHLISLFTEENEQKEFFLSKEIFEKILIDNNLLNHLNSLLKDLLNEA
RGKFCTIDDLNSVILVGGGTQIPLIKEWIANEISGIQIRTPPPIESIAVGALAMTPGVKIKDILIKGISIRLFNKREQKQ
FWHPIFYKGQTWPTEKPFELILQARKDGQSIFEIIIGETKEKRNYDVIFENGLPKLSEYQNEEEVLKWNKKPLKIKLNNE
CKIGEDCLKLYFSITKDSSLYVKYLDINEKELGESNLGNIF

Specific function: Acts as a chaperone [H]

COG id: COG0443

COG function: function code O; Molecular chaperone

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the heat shock protein 70 family [H]

Homologues:

Organism=Escherichia coli, GI1786196, Length=487, Percent_Identity=21.5605749486653, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012725
- InterPro:   IPR018181
- InterPro:   IPR001023
- InterPro:   IPR013126 [H]

Pfam domain/function: PF00012 HSP70 [H]

EC number: NA

Molecular weight: Translated: 58600; Mature: 58600

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00329 HSP70_2 ; PS01036 HSP70_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKNFSGTLALDLGNTNTVIAFQDEKGKDLNLIEIPGITSSPGVIPTVIWFEGEDNIAKI
CCCCCCCEEEEEECCCEEEEEEECCCCCCCCEEEECCCCCCCCCEEEEEEECCCCCHHHH
GLSALKKKNLSNSETYFHSNFKRLIANPVERLQKKVLSPNESGEKFFKILWENIPNELEI
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEE
KRLVLTAPIDTYKGYRKWLINLCESLPINEIALVDEPTAAGIGINVPLGSIIMIIDIGGS
EEEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCEEEECCCCCEEEEEECCCC
TIDMSVIKTQGGEGKSAPIAELLKFQGKDVSSISKQKIRCAETISKSGSKIGGKDIDQWI
EEEEEEEEECCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
VNYFLPSNQDERNLSIAEKLKCKLSSPGVESETRHLISLFTEENEQKEFFLSKEIFEKIL
HHHHCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
IDNNLLNHLNSLLKDLLNEARGKFCTIDDLNSVILVGGGTQIPLIKEWIANEISGIQIRT
HCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEECCCCCHHHHHHHHHCCCCEEEEC
PPPIESIAVGALAMTPGVKIKDILIKGISIRLFNKREQKQFWHPIFYKGQTWPTEKPFEL
CCCHHHHHHHHEEECCCCEEEHHHHCCCEEEEECCHHHHHHCCCEEECCCCCCCCCHHHH
ILQARKDGQSIFEIIIGETKEKRNYDVIFENGLPKLSEYQNEEEVLKWNKKPLKIKLNNE
HHHHHHCHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHCCHHHHHHCCCCCEEEEECCC
CKIGEDCLKLYFSITKDSSLYVKYLDINEKELGESNLGNIF
CCCCHHHHHHHHHCCCCCCEEEEEEECCHHHCCCCCCCCCC
>Mature Secondary Structure
MEKNFSGTLALDLGNTNTVIAFQDEKGKDLNLIEIPGITSSPGVIPTVIWFEGEDNIAKI
CCCCCCCEEEEEECCCEEEEEEECCCCCCCCEEEECCCCCCCCCEEEEEEECCCCCHHHH
GLSALKKKNLSNSETYFHSNFKRLIANPVERLQKKVLSPNESGEKFFKILWENIPNELEI
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEE
KRLVLTAPIDTYKGYRKWLINLCESLPINEIALVDEPTAAGIGINVPLGSIIMIIDIGGS
EEEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCEEEECCCCCEEEEEECCCC
TIDMSVIKTQGGEGKSAPIAELLKFQGKDVSSISKQKIRCAETISKSGSKIGGKDIDQWI
EEEEEEEEECCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
VNYFLPSNQDERNLSIAEKLKCKLSSPGVESETRHLISLFTEENEQKEFFLSKEIFEKIL
HHHHCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
IDNNLLNHLNSLLKDLLNEARGKFCTIDDLNSVILVGGGTQIPLIKEWIANEISGIQIRT
HCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEECCCCCHHHHHHHHHCCCCEEEEC
PPPIESIAVGALAMTPGVKIKDILIKGISIRLFNKREQKQFWHPIFYKGQTWPTEKPFEL
CCCHHHHHHHHEEECCCCEEEHHHHCCCEEEEECCHHHHHHCCCEEECCCCCCCCCHHHH
ILQARKDGQSIFEIIIGETKEKRNYDVIFENGLPKLSEYQNEEEVLKWNKKPLKIKLNNE
HHHHHHCHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHCCHHHHHHCCCCCEEEEECCC
CKIGEDCLKLYFSITKDSSLYVKYLDINEKELGESNLGNIF
CCCCHHHHHHHHHCCCCCCEEEEEEECCHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA