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The map label for this gene is opuD [H]

Identifier: 123966836

GI number: 123966836

Start: 1401973

End: 1403532

Strand: Reverse

Name: opuD [H]

Synonym: P9515_16031

Alternate gene names: 123966836

Gene position: 1403532-1401973 (Counterclockwise)

Preceding gene: 123966838

Following gene: 123966826

Centisome position: 82.36

GC content: 33.85

Gene sequence:

>1560_bases
ATGTATAAAGCTATAAATAGTGCGAAAGCATTTTTAAGTGATCAATCTTTACGAAGTAAATCACTAATAATAGGGGGCTT
GCCATTAATTATATTTCTGGCTATTTGTTCAATAAATTTAAATCTTGCTGAAAAAATTACTAATTATGGCAAAGACTTTG
CTTTGACAAATTTTGGTTCTACTTGGCTAATAATGGTGGTTGGGATATGCATTGTTGGGTTCTTTTTAGGATTTTCTCCT
CTTGGCTCTTTAAGAATTGGTGGACCTGATGTAAAACCATCTCTGAATTTTTTTGATTGGTGTGCGGTTTTAATATGTAC
TCTTCTTGCCGGTGGTGGAGTTTTTTGGTCTGCTGCAGAACCTTTGATTCACTTCTTAAGTCCCGCTAGTTATTTTTCTG
ATATTACGGGTGGAACAGAACAGGCTATTGATCCTTCTTTAGCAGTAAGTTTTCTCCACTGGGGTTTTCTTGCTTGGGGA
TTAGTTGCAAGTACTGTAACTATAACTTTCTCTTTGCTTTCTCAAAATGGGTACCCTCTTAGACCAAGAAGTTTATTAAT
ACCAATATTCCCAAAAAGAATTGTTGAAGGATTAGTTGGAGATATTACTGATGGATTATCCGTCGTTGCAGCGGTAGCAG
GAACTGTGGGCCCTTTAGGATTTTTATCACTTCAATTAAGTAATGCTGCTGGAAAATTATCTTTTTTGTCAAATAATAGT
TTTTTACAAACTTTGATAGTTTTGGTCTTGACTTTGATTTTCACAATTTCAACCTTGAGCGGTATTCAAAAAGGTATTAA
GTTTCTTTCTGAAATTAATATTTGGCTTACCATTGTTCTAGGCGTAATACTTTTATTGATTGGACCTACTTTTTGGTTAG
TTAATCATTTTCTTAGTAGTAATTTTTTATACTTGAGTGATGTTAAAAAACTTGCATTACCAAATCCTAATGATGCTTGG
GTCAAGGGCTGGACTATTTTTTATTGGGGATGGTTTTTAGGTTATGCACCTTTAATGGGATTGTTTGCCGCTGGTGTTAG
TAAAGGTCGTACTTTTAGAGAATTAATAATTGTAGTTTGTATTATTTGTCCATTTGTGACTAATTTGTGGTTCACAATCC
TTGGAGGTAATGGAATATTTCTTGAATTAAATAATCCTAATTTATTAGGAAAAGAGTTAGTAGAGGGCGGAGAAGCAGGC
GTATTATTCTCAATTTTAAGTCAGTTGCCTTTATCGTTTTTAATGATACCAATTTCAATATTACTTATAGTCTTATTCAT
GTGTACGAGTGCTGATTCAATAAGTTATGCAGCTGCAATTGTCGTTAGTGGTAAGGAAAATCCTCCAAAAAAGATAAGAT
TATTTTGGGCATTGATGATAGGTTTTCTTACAATAGCTCTTTTAAGAATCGGATCAGAAGTTGGAGATAAAACAAGTATC
GATGCTCTTCAAGCATTTATTGTCATAACTGCAGTCCCTGTCACTCCCTTAATAATTTCCACCATATGGACAGCTCCGAA
ACTTGCAATAAAAGAATATGTAAGGTTAAAAAAAGGTTAG

Upstream 100 bases:

>100_bases
ATAATCTGACATTTCAATTGCCATAATAAATTATTTACTATCCAAGTTGCCTTGCTCTCTATTCTTATTTTGTTTAAAGT
AACAAAAGATTTGAAAATTT

Downstream 100 bases:

>100_bases
TTTAAAATAAAATATAAATTACAGTGGGTGCTTTGGGAGCACTAGGTCGCAGGTTCTAATCCTGTCGCCCTGATCAGTAA
AAACCTAAATTATGCAGTAA

Product: hypothetical protein

Products: Proton [Cytoplasm]; choline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 519; Mature: 519

Protein sequence:

>519_residues
MYKAINSAKAFLSDQSLRSKSLIIGGLPLIIFLAICSINLNLAEKITNYGKDFALTNFGSTWLIMVVGICIVGFFLGFSP
LGSLRIGGPDVKPSLNFFDWCAVLICTLLAGGGVFWSAAEPLIHFLSPASYFSDITGGTEQAIDPSLAVSFLHWGFLAWG
LVASTVTITFSLLSQNGYPLRPRSLLIPIFPKRIVEGLVGDITDGLSVVAAVAGTVGPLGFLSLQLSNAAGKLSFLSNNS
FLQTLIVLVLTLIFTISTLSGIQKGIKFLSEINIWLTIVLGVILLLIGPTFWLVNHFLSSNFLYLSDVKKLALPNPNDAW
VKGWTIFYWGWFLGYAPLMGLFAAGVSKGRTFRELIIVVCIICPFVTNLWFTILGGNGIFLELNNPNLLGKELVEGGEAG
VLFSILSQLPLSFLMIPISILLIVLFMCTSADSISYAAAIVVSGKENPPKKIRLFWALMIGFLTIALLRIGSEVGDKTSI
DALQAFIVITAVPVTPLIISTIWTAPKLAIKEYVRLKKG

Sequences:

>Translated_519_residues
MYKAINSAKAFLSDQSLRSKSLIIGGLPLIIFLAICSINLNLAEKITNYGKDFALTNFGSTWLIMVVGICIVGFFLGFSP
LGSLRIGGPDVKPSLNFFDWCAVLICTLLAGGGVFWSAAEPLIHFLSPASYFSDITGGTEQAIDPSLAVSFLHWGFLAWG
LVASTVTITFSLLSQNGYPLRPRSLLIPIFPKRIVEGLVGDITDGLSVVAAVAGTVGPLGFLSLQLSNAAGKLSFLSNNS
FLQTLIVLVLTLIFTISTLSGIQKGIKFLSEINIWLTIVLGVILLLIGPTFWLVNHFLSSNFLYLSDVKKLALPNPNDAW
VKGWTIFYWGWFLGYAPLMGLFAAGVSKGRTFRELIIVVCIICPFVTNLWFTILGGNGIFLELNNPNLLGKELVEGGEAG
VLFSILSQLPLSFLMIPISILLIVLFMCTSADSISYAAAIVVSGKENPPKKIRLFWALMIGFLTIALLRIGSEVGDKTSI
DALQAFIVITAVPVTPLIISTIWTAPKLAIKEYVRLKKG
>Mature_519_residues
MYKAINSAKAFLSDQSLRSKSLIIGGLPLIIFLAICSINLNLAEKITNYGKDFALTNFGSTWLIMVVGICIVGFFLGFSP
LGSLRIGGPDVKPSLNFFDWCAVLICTLLAGGGVFWSAAEPLIHFLSPASYFSDITGGTEQAIDPSLAVSFLHWGFLAWG
LVASTVTITFSLLSQNGYPLRPRSLLIPIFPKRIVEGLVGDITDGLSVVAAVAGTVGPLGFLSLQLSNAAGKLSFLSNNS
FLQTLIVLVLTLIFTISTLSGIQKGIKFLSEINIWLTIVLGVILLLIGPTFWLVNHFLSSNFLYLSDVKKLALPNPNDAW
VKGWTIFYWGWFLGYAPLMGLFAAGVSKGRTFRELIIVVCIICPFVTNLWFTILGGNGIFLELNNPNLLGKELVEGGEAG
VLFSILSQLPLSFLMIPISILLIVLFMCTSADSISYAAAIVVSGKENPPKKIRLFWALMIGFLTIALLRIGSEVGDKTSI
DALQAFIVITAVPVTPLIISTIWTAPKLAIKEYVRLKKG

Specific function: High-affinity uptake of glycine betaine [H]

COG id: COG1292

COG function: function code M; Choline-glycine betaine transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCCT transporter (TC 2.A.15) family [H]

Homologues:

Organism=Escherichia coli, GI1786506, Length=474, Percent_Identity=27.0042194092827, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI1788102, Length=433, Percent_Identity=24.4803695150115, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1786224, Length=471, Percent_Identity=25.4777070063694, Blast_Score=132, Evalue=5e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000060
- InterPro:   IPR018093 [H]

Pfam domain/function: PF02028 BCCT [H]

EC number: NA

Molecular weight: Translated: 56153; Mature: 56153

Theoretical pI: Translated: 8.99; Mature: 8.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKAINSAKAFLSDQSLRSKSLIIGGLPLIIFLAICSINLNLAEKITNYGKDFALTNFGS
CCCCHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHCCCHHHHHHHHCCCCCEECCCCH
TWLIMVVGICIVGFFLGFSPLGSLRIGGPDVKPSLNFFDWCAVLICTLLAGGGVFWSAAE
HHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHCCCEEHHHHH
PLIHFLSPASYFSDITGGTEQAIDPSLAVSFLHWGFLAWGLVASTVTITFSLLSQNGYPL
HHHHHHCHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
RPRSLLIPIFPKRIVEGLVGDITDGLSVVAAVAGTVGPLGFLSLQLSNAAGKLSFLSNNS
CCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHEEEECCCCCCEEEECCCH
FLQTLIVLVLTLIFTISTLSGIQKGIKFLSEINIWLTIVLGVILLLIGPTFWLVNHFLSS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NFLYLSDVKKLALPNPNDAWVKGWTIFYWGWFLGYAPLMGLFAAGVSKGRTFRELIIVVC
CCEEEHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
IICPFVTNLWFTILGGNGIFLELNNPNLLGKELVEGGEAGVLFSILSQLPLSFLMIPISI
HHHHHHHHHHHHEECCCEEEEEECCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
LLIVLFMCTSADSISYAAAIVVSGKENPPKKIRLFWALMIGFLTIALLRIGSEVGDKTSI
HHHHHHHHCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
DALQAFIVITAVPVTPLIISTIWTAPKLAIKEYVRLKKG
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MYKAINSAKAFLSDQSLRSKSLIIGGLPLIIFLAICSINLNLAEKITNYGKDFALTNFGS
CCCCHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHCCCHHHHHHHHCCCCCEECCCCH
TWLIMVVGICIVGFFLGFSPLGSLRIGGPDVKPSLNFFDWCAVLICTLLAGGGVFWSAAE
HHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHCCCEEHHHHH
PLIHFLSPASYFSDITGGTEQAIDPSLAVSFLHWGFLAWGLVASTVTITFSLLSQNGYPL
HHHHHHCHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
RPRSLLIPIFPKRIVEGLVGDITDGLSVVAAVAGTVGPLGFLSLQLSNAAGKLSFLSNNS
CCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHEEEECCCCCCEEEECCCH
FLQTLIVLVLTLIFTISTLSGIQKGIKFLSEINIWLTIVLGVILLLIGPTFWLVNHFLSS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NFLYLSDVKKLALPNPNDAWVKGWTIFYWGWFLGYAPLMGLFAAGVSKGRTFRELIIVVC
CCEEEHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
IICPFVTNLWFTILGGNGIFLELNNPNLLGKELVEGGEAGVLFSILSQLPLSFLMIPISI
HHHHHHHHHHHHEECCCEEEEEECCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
LLIVLFMCTSADSISYAAAIVVSGKENPPKKIRLFWALMIGFLTIALLRIGSEVGDKTSI
HHHHHHHHCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
DALQAFIVITAVPVTPLIISTIWTAPKLAIKEYVRLKKG
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; choline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + choline [Periplasm] = Proton [Cytoplasm] + choline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8752321; 9387221; 9384377 [H]