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The map label for this gene is purD [H]

Identifier: 123966734

GI number: 123966734

Start: 1335790

End: 1337127

Strand: Direct

Name: purD [H]

Synonym: P9515_15011

Alternate gene names: 123966734

Gene position: 1335790-1337127 (Clockwise)

Preceding gene: 123966726

Following gene: 123966735

Centisome position: 78.38

GC content: 30.94

Gene sequence:

>1338_bases
ATGAGTATTAATTCAAACAACTCTAAAAATTCTAATAAATTAGAAAATATTTTAATAATTGGTAATGGTGGAAGAGAAAA
TGCATTGGCATGGGCCATTCAAAAAAACGAATTAATCAAAAGAATTTATTTACTTCCAGGAAATGCTGGCTCAAAAAATA
TAAATAAATCTGAGAGAATAAATTTAGATTTAAATAATATTGAAGCATTAATAAAAAAACTGAAATCACTAAATATTGAT
TTAGTAGTAATAGGTCCAGAGACTTATTTAGCAGATGGATTAGGAGGAATTCTTCGAAAAAACAATTTTGATGTATTTGG
ACCTGGCTCAGATGGAGCCAAATTAGAATCAAGCAAATCTTGGGCGAAGGAATTTATGAGAGAGGCAAAAATCCCTACTG
CAAATTTCTGGAAAATTAAATCATTAGAGGAAGCAAAAGAAATTATTTTTAAATCTCCAAATCCATTAGTAGTAAAAGCT
GATGGATTAGCTTCAGGGAAAGGAGTTTATATCCCTGAGACAAAAGAGTCATCTCTTCAAGCTACGGAAGAAATATTTAA
AGGTAAGTTTGGCAATGCTGGAAAAACAATTGTTTTGGAAGAAAAAATTGAAGGTCCAGAAGTCTCAGTTTTTGCTCTTT
GTGATGGGAAAAAATACGTTTTACTTCCTACAGCACAAGATCATAAAAGACTTAACGAGAATGATAAAGGGCCTAATACA
GGGGGTATGGGAGCCTATTCTCCTACTCCACTCATAACAAAAACTAATCTTGAAAAGATTACAAATGAAATCATTGAGCC
AACAATTAATACATTGTTGAAAAAGAATATTGACTACAGAGGCGTTTTATATTTTGGACTAATGATCACAAAATCAGGTC
CGAAAGTTATAGAATACAATTGTAGGTTTGGTGATCCGGAATGCCAAACGATAATGCCTTTATTGGATGAAAATTTTGTG
ATTCTTTTACAAAAATGTTCAAAAGGCAATCTTATCGGTAACGAAACAATTAAAATGCCTGATAAATTTAGTGGTTGTGT
AATCGCAACTTCAAAAGGTTATCCTCTTAAATACGAAATTGGTTTTCCTATAGATTTCGGGAATATTGATAAAGAAGATT
GTCAAATTTTTGATTCGGGCACATCTTTAAATTCAAATGGTGAAGTTATAACTAATGGTGGAAGAGTACTTAGTATTGTT
TGTCAAGGTAAGGATTTCGACAAAGTTTTTGAAAAAGCATACAAAAATTTGAAAGAGATAAGTTTTGAAGGGATTTTTTA
TAGAAAAGATATTGGTCATCAAGTCAGAACAAAAACTTTAGATGACGAGATTAATTAA

Upstream 100 bases:

>100_bases
AATTAGACTGTTTACTGGCATTAGAAACGTTTTATAGTTTTATTAATACAATTTTAACTGATTTTTCTTCCAAACCTTAA
TATATTTAATAAACGAATTC

Downstream 100 bases:

>100_bases
ATGATAAATAGCAATGAAACTAAAAAAATAGAAGTAAGTTCCTCAAACCAAACTATTGAGGATGATAGTTACGAATCTCT
TTCAAATAGAGTTTTAACAT

Product: phosphoribosylglycinamide synthetase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 445; Mature: 444

Protein sequence:

>445_residues
MSINSNNSKNSNKLENILIIGNGGRENALAWAIQKNELIKRIYLLPGNAGSKNINKSERINLDLNNIEALIKKLKSLNID
LVVIGPETYLADGLGGILRKNNFDVFGPGSDGAKLESSKSWAKEFMREAKIPTANFWKIKSLEEAKEIIFKSPNPLVVKA
DGLASGKGVYIPETKESSLQATEEIFKGKFGNAGKTIVLEEKIEGPEVSVFALCDGKKYVLLPTAQDHKRLNENDKGPNT
GGMGAYSPTPLITKTNLEKITNEIIEPTINTLLKKNIDYRGVLYFGLMITKSGPKVIEYNCRFGDPECQTIMPLLDENFV
ILLQKCSKGNLIGNETIKMPDKFSGCVIATSKGYPLKYEIGFPIDFGNIDKEDCQIFDSGTSLNSNGEVITNGGRVLSIV
CQGKDFDKVFEKAYKNLKEISFEGIFYRKDIGHQVRTKTLDDEIN

Sequences:

>Translated_445_residues
MSINSNNSKNSNKLENILIIGNGGRENALAWAIQKNELIKRIYLLPGNAGSKNINKSERINLDLNNIEALIKKLKSLNID
LVVIGPETYLADGLGGILRKNNFDVFGPGSDGAKLESSKSWAKEFMREAKIPTANFWKIKSLEEAKEIIFKSPNPLVVKA
DGLASGKGVYIPETKESSLQATEEIFKGKFGNAGKTIVLEEKIEGPEVSVFALCDGKKYVLLPTAQDHKRLNENDKGPNT
GGMGAYSPTPLITKTNLEKITNEIIEPTINTLLKKNIDYRGVLYFGLMITKSGPKVIEYNCRFGDPECQTIMPLLDENFV
ILLQKCSKGNLIGNETIKMPDKFSGCVIATSKGYPLKYEIGFPIDFGNIDKEDCQIFDSGTSLNSNGEVITNGGRVLSIV
CQGKDFDKVFEKAYKNLKEISFEGIFYRKDIGHQVRTKTLDDEIN
>Mature_444_residues
SINSNNSKNSNKLENILIIGNGGRENALAWAIQKNELIKRIYLLPGNAGSKNINKSERINLDLNNIEALIKKLKSLNIDL
VVIGPETYLADGLGGILRKNNFDVFGPGSDGAKLESSKSWAKEFMREAKIPTANFWKIKSLEEAKEIIFKSPNPLVVKAD
GLASGKGVYIPETKESSLQATEEIFKGKFGNAGKTIVLEEKIEGPEVSVFALCDGKKYVLLPTAQDHKRLNENDKGPNTG
GMGAYSPTPLITKTNLEKITNEIIEPTINTLLKKNIDYRGVLYFGLMITKSGPKVIEYNCRFGDPECQTIMPLLDENFVI
LLQKCSKGNLIGNETIKMPDKFSGCVIATSKGYPLKYEIGFPIDFGNIDKEDCQIFDSGTSLNSNGEVITNGGRVLSIVC
QGKDFDKVFEKAYKNLKEISFEGIFYRKDIGHQVRTKTLDDEIN

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=423, Percent_Identity=43.0260047281324, Blast_Score=347, Evalue=1e-95,
Organism=Homo sapiens, GI4503915, Length=420, Percent_Identity=43.3333333333333, Blast_Score=347, Evalue=1e-95,
Organism=Homo sapiens, GI209869995, Length=420, Percent_Identity=43.3333333333333, Blast_Score=347, Evalue=1e-95,
Organism=Homo sapiens, GI209869993, Length=420, Percent_Identity=43.3333333333333, Blast_Score=347, Evalue=1e-95,
Organism=Escherichia coli, GI1790438, Length=420, Percent_Identity=38.3333333333333, Blast_Score=306, Evalue=3e-84,
Organism=Caenorhabditis elegans, GI17567511, Length=432, Percent_Identity=39.8148148148148, Blast_Score=289, Evalue=2e-78,
Organism=Saccharomyces cerevisiae, GI6321203, Length=437, Percent_Identity=37.2997711670481, Blast_Score=287, Evalue=3e-78,
Organism=Drosophila melanogaster, GI62471619, Length=434, Percent_Identity=42.1658986175115, Blast_Score=311, Evalue=4e-85,
Organism=Drosophila melanogaster, GI24582400, Length=431, Percent_Identity=42.4593967517401, Blast_Score=310, Evalue=2e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 49221; Mature: 49090

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSINSNNSKNSNKLENILIIGNGGRENALAWAIQKNELIKRIYLLPGNAGSKNINKSERI
CCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEEHHHHEEEEEEECCCCCCCCCCCCCEE
NLDLNNIEALIKKLKSLNIDLVVIGPETYLADGLGGILRKNNFDVFGPGSDGAKLESSKS
EEEHHHHHHHHHHHHHCCEEEEEECCCHHHHHCHHHHHCCCCCEEECCCCCCCCCCCHHH
WAKEFMREAKIPTANFWKIKSLEEAKEIIFKSPNPLVVKADGLASGKGVYIPETKESSLQ
HHHHHHHHHCCCCCCEEEECCHHHHHHHHCCCCCCEEEEECCCCCCCEEECCCCCCHHHH
ATEEIFKGKFGNAGKTIVLEEKIEGPEVSVFALCDGKKYVLLPTAQDHKRLNENDKGPNT
HHHHHHCCCCCCCCCEEEEEECCCCCCEEEEEEECCCEEEEECCCHHHHHCCCCCCCCCC
GGMGAYSPTPLITKTNLEKITNEIIEPTINTLLKKNIDYRGVLYFGLMITKSGPKVIEYN
CCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCEEEEEE
CRFGDPECQTIMPLLDENFVILLQKCSKGNLIGNETIKMPDKFSGCVIATSKGYPLKYEI
CCCCCCHHHHHHHHHCCCEEEEEEECCCCCEECCCEEECCCCCCCEEEEECCCCEEEEEE
GFPIDFGNIDKEDCQIFDSGTSLNSNGEVITNGGRVLSIVCQGKDFDKVFEKAYKNLKEI
CCEECCCCCCHHHCEEEECCCCCCCCCCEEECCCEEEEEEECCCCHHHHHHHHHHHHHHC
SFEGIFYRKDIGHQVRTKTLDDEIN
CCCEEEEECCCCCHHHHCCCCCCCC
>Mature Secondary Structure 
SINSNNSKNSNKLENILIIGNGGRENALAWAIQKNELIKRIYLLPGNAGSKNINKSERI
CCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEEHHHHEEEEEEECCCCCCCCCCCCCEE
NLDLNNIEALIKKLKSLNIDLVVIGPETYLADGLGGILRKNNFDVFGPGSDGAKLESSKS
EEEHHHHHHHHHHHHHCCEEEEEECCCHHHHHCHHHHHCCCCCEEECCCCCCCCCCCHHH
WAKEFMREAKIPTANFWKIKSLEEAKEIIFKSPNPLVVKADGLASGKGVYIPETKESSLQ
HHHHHHHHHCCCCCCEEEECCHHHHHHHHCCCCCCEEEEECCCCCCCEEECCCCCCHHHH
ATEEIFKGKFGNAGKTIVLEEKIEGPEVSVFALCDGKKYVLLPTAQDHKRLNENDKGPNT
HHHHHHCCCCCCCCCEEEEEECCCCCCEEEEEEECCCEEEEECCCHHHHHCCCCCCCCCC
GGMGAYSPTPLITKTNLEKITNEIIEPTINTLLKKNIDYRGVLYFGLMITKSGPKVIEYN
CCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCEEEEEE
CRFGDPECQTIMPLLDENFVILLQKCSKGNLIGNETIKMPDKFSGCVIATSKGYPLKYEI
CCCCCCHHHHHHHHHCCCEEEEEEECCCCCEECCCEEECCCCCCCEEEEECCCCEEEEEE
GFPIDFGNIDKEDCQIFDSGTSLNSNGEVITNGGRVLSIVCQGKDFDKVFEKAYKNLKEI
CCEECCCCCCHHHCEEEECCCCCCCCCCEEECCCEEEEEEECCCCHHHHHHHHHHHHHHC
SFEGIFYRKDIGHQVRTKTLDDEIN
CCCEEEEECCCCCHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]