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The map label for this gene is glgX [H]

Identifier: 123966716

GI number: 123966716

Start: 1317851

End: 1319857

Strand: Direct

Name: glgX [H]

Synonym: P9515_14831

Alternate gene names: 123966716

Gene position: 1317851-1319857 (Clockwise)

Preceding gene: 123966711

Following gene: 123966717

Centisome position: 77.33

GC content: 31.64

Gene sequence:

>2007_bases
TTGCCTCTAGGAAGTTCAATAACATCAAAAGGAGTCAATTTCTCTTTAATAGCTACAAACGCAGAATATATAGAAATTTT
AGTATTTGAGAAAGAAGATTCAGTTGCTCCAAAAGCAATCTTCAAATTAGATCAAAATCATAAGACTGGGCCTTACTGGC
ATGCGGAAATAAACGATCTCAAGGAAGGATCTATCTACGCCTTCAGAATAAAGCAGAAGGTAAACGAGCCTAATAAAGAT
TATTCTAAAACAGTTTTAATTGATCCTTGTTCAAGAGGCATTACAGGATGGAGTAAATACTCTAGAAAAAATGCGCTAAA
TAATTTTGATAACATGGATTCATGTCTCAAAAGTGTTGTTTGTGATAGGAAAGTTTTTAATTTTAAAAATTTCCCTAGAC
CAAAACACTCTTGGGAAGAAACAATAATTTATGAGCTCCATATCAAAGCATTTACTCAATCCTCTAATGAAGATGAAAGT
TGCTTTAAAAAATTTTTAAAAAAAATTCCATATCTAAAAGAACTTGGCATAACAAGTATTGAATTACTTCCAGTATTTTG
TTTTGACCCAAATGATGCTCCTAATGGATTAGAAAATTTTTGGGGTTATAGTCCTATTAACTGGTTTACTCCTCATTTTC
AATATCTTTCAAATAAATCTGCCGAACAAAATAGAGAAGAATTTAGAAAATTTGTGGAAGAATGCCATAAAGAAAATATT
GAGGTTATTTTAGATGTGGTATACAACCATACCTCTGAAGGCGATTCCGGGGGGCCAGCAATATCTTGGAAAGGAATAGA
TGAAAATCTTTACTATTTCATCGATAAAGATAAAAATTATCAGGATGTTACTGGTTGTGGCAATACAATTGCAGCAAATA
GAGGGTTAGTTAGAAAATTAATAATAGAGTCATTAAAGTGCTGGGCAAATGAATTTGGCGTTGATGGTTTTAGATTTGAT
TTAGGAATTGCCTTATCTAGAGGAGAAGATCTTGTCCCACTTGATAATCCACCAATTTTTGAAGATATTGAATGTGAGCC
TGAACTTGCCGATATAAAACTAATTAGTGAGCCTTGGGATTGTGGAGGATTATACAAATTAAATAATTTCCCTTCAAAGA
AAACGTTTACATGGAACGGTCATTTCAGAGATGATTTAAGAAGATTTTGGAGAGGGGATAAAGATTCGGCTTGGAATATG
AGTGACAAAATCAAAGGCAGTCCATCACATTACAAAGAGAATATTTCTTTCCCAAAGTCTATTAATTTCATAACTTCTCA
CGATGGATTTACACTAAAAGATTTAGTTTCTTTTAATAAAAAGCATAATTTTGCAAATAAAGAACAAAATAGAGATGGAG
ATAACCACAACAATTCATGGAATCATGGAGTGGAAGGACCTACATCAAACTTATTTATTAAAAAATTAAGAAAGCGGCAA
CAAAAAAATTTCCTTTTAAGTTTATTAATCTCAAAAGGAGTCCCTATGATACTAATGGGTGATGAAATCGGAAGATCTCA
GGGCGGTAATAACAATTCTTGGTGCCAAAATAATTCCTTGGGTTGGATGAATTGGGATGAAAGCCAACAAGATTTAGAGT
TATTAACTTATCTTAAATATCTAATAAAAATCAGAAAAAAATTTATTAAATTTTTTAATCCTATCTCTCCACAAAATAGA
AAAGATATTGATAATTTAGTTAATTACTACTGGCATGGTGCAAAGTTAGAAAGCCCAGATTGGAGTAGCTGGTCACATAC
AATTGCTTTTAGTATGAATAAAGGTAAAAATAATCCTTTAATATGGGCAGGCCTAAATGCATATACAAAAAGTATTGATT
TTCACCTGCCAGAATCTAAAAGCAAATGGCTTAAAGTTGTTGATACTAACGAGTCTGGAACTATCATGCCTATTCCCCTA
GAACATAAATCTATTACAGTAGAAAATAGGAGCTCTGTATTAATTATTTCAGAAGAAGTATTTGGAACAAAAAATATTAT
ATTCTAA

Upstream 100 bases:

>100_bases
CTTATGATTCAGATGTCTGGATAATTTTATTAAATTTGTTCATTCATAAAAATATCAAAGAAATAACGACACAGTGAATG
ATATAAAGCTTGGGAACCCA

Downstream 100 bases:

>100_bases
AAGCGGGCGGCGGGAATCGAACCCGCATCTTCAGCTTGGAAGGCTGAGGTTTTACCACTAAACCACGCCCGCAATAAGTA
ATAGAATTACATTTGCAATA

Product: putative isoamylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 668; Mature: 667

Protein sequence:

>668_residues
MPLGSSITSKGVNFSLIATNAEYIEILVFEKEDSVAPKAIFKLDQNHKTGPYWHAEINDLKEGSIYAFRIKQKVNEPNKD
YSKTVLIDPCSRGITGWSKYSRKNALNNFDNMDSCLKSVVCDRKVFNFKNFPRPKHSWEETIIYELHIKAFTQSSNEDES
CFKKFLKKIPYLKELGITSIELLPVFCFDPNDAPNGLENFWGYSPINWFTPHFQYLSNKSAEQNREEFRKFVEECHKENI
EVILDVVYNHTSEGDSGGPAISWKGIDENLYYFIDKDKNYQDVTGCGNTIAANRGLVRKLIIESLKCWANEFGVDGFRFD
LGIALSRGEDLVPLDNPPIFEDIECEPELADIKLISEPWDCGGLYKLNNFPSKKTFTWNGHFRDDLRRFWRGDKDSAWNM
SDKIKGSPSHYKENISFPKSINFITSHDGFTLKDLVSFNKKHNFANKEQNRDGDNHNNSWNHGVEGPTSNLFIKKLRKRQ
QKNFLLSLLISKGVPMILMGDEIGRSQGGNNNSWCQNNSLGWMNWDESQQDLELLTYLKYLIKIRKKFIKFFNPISPQNR
KDIDNLVNYYWHGAKLESPDWSSWSHTIAFSMNKGKNNPLIWAGLNAYTKSIDFHLPESKSKWLKVVDTNESGTIMPIPL
EHKSITVENRSSVLIISEEVFGTKNIIF

Sequences:

>Translated_668_residues
MPLGSSITSKGVNFSLIATNAEYIEILVFEKEDSVAPKAIFKLDQNHKTGPYWHAEINDLKEGSIYAFRIKQKVNEPNKD
YSKTVLIDPCSRGITGWSKYSRKNALNNFDNMDSCLKSVVCDRKVFNFKNFPRPKHSWEETIIYELHIKAFTQSSNEDES
CFKKFLKKIPYLKELGITSIELLPVFCFDPNDAPNGLENFWGYSPINWFTPHFQYLSNKSAEQNREEFRKFVEECHKENI
EVILDVVYNHTSEGDSGGPAISWKGIDENLYYFIDKDKNYQDVTGCGNTIAANRGLVRKLIIESLKCWANEFGVDGFRFD
LGIALSRGEDLVPLDNPPIFEDIECEPELADIKLISEPWDCGGLYKLNNFPSKKTFTWNGHFRDDLRRFWRGDKDSAWNM
SDKIKGSPSHYKENISFPKSINFITSHDGFTLKDLVSFNKKHNFANKEQNRDGDNHNNSWNHGVEGPTSNLFIKKLRKRQ
QKNFLLSLLISKGVPMILMGDEIGRSQGGNNNSWCQNNSLGWMNWDESQQDLELLTYLKYLIKIRKKFIKFFNPISPQNR
KDIDNLVNYYWHGAKLESPDWSSWSHTIAFSMNKGKNNPLIWAGLNAYTKSIDFHLPESKSKWLKVVDTNESGTIMPIPL
EHKSITVENRSSVLIISEEVFGTKNIIF
>Mature_667_residues
PLGSSITSKGVNFSLIATNAEYIEILVFEKEDSVAPKAIFKLDQNHKTGPYWHAEINDLKEGSIYAFRIKQKVNEPNKDY
SKTVLIDPCSRGITGWSKYSRKNALNNFDNMDSCLKSVVCDRKVFNFKNFPRPKHSWEETIIYELHIKAFTQSSNEDESC
FKKFLKKIPYLKELGITSIELLPVFCFDPNDAPNGLENFWGYSPINWFTPHFQYLSNKSAEQNREEFRKFVEECHKENIE
VILDVVYNHTSEGDSGGPAISWKGIDENLYYFIDKDKNYQDVTGCGNTIAANRGLVRKLIIESLKCWANEFGVDGFRFDL
GIALSRGEDLVPLDNPPIFEDIECEPELADIKLISEPWDCGGLYKLNNFPSKKTFTWNGHFRDDLRRFWRGDKDSAWNMS
DKIKGSPSHYKENISFPKSINFITSHDGFTLKDLVSFNKKHNFANKEQNRDGDNHNNSWNHGVEGPTSNLFIKKLRKRQQ
KNFLLSLLISKGVPMILMGDEIGRSQGGNNNSWCQNNSLGWMNWDESQQDLELLTYLKYLIKIRKKFIKFFNPISPQNRK
DIDNLVNYYWHGAKLESPDWSSWSHTIAFSMNKGKNNPLIWAGLNAYTKSIDFHLPESKSKWLKVVDTNESGTIMPIPLE
HKSITVENRSSVLIISEEVFGTKNIIF

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=564, Percent_Identity=38.2978723404255, Blast_Score=359, Evalue=1e-100,
Organism=Escherichia coli, GI1789839, Length=183, Percent_Identity=27.8688524590164, Blast_Score=63, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 77055; Mature: 76924

Theoretical pI: Translated: 7.40; Mature: 7.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLGSSITSKGVNFSLIATNAEYIEILVFEKEDSVAPKAIFKLDQNHKTGPYWHAEINDL
CCCCCCCCCCCCEEEEEEECCCEEEEEEEECCCCCCCHHHHEECCCCCCCCEEECCCCCC
KEGSIYAFRIKQKVNEPNKDYSKTVLIDPCSRGITGWSKYSRKNALNNFDNMDSCLKSVV
CCCCEEEEEEEHHCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHH
CDRKVFNFKNFPRPKHSWEETIIYELHIKAFTQSSNEDESCFKKFLKKIPYLKELGITSI
HHHHHHCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCHHHHHHHHHHHCCCHHHCCCCEE
ELLPVFCFDPNDAPNGLENFWGYSPINWFTPHFQYLSNKSAEQNREEFRKFVEECHKENI
EEEEEEEECCCCCCCHHHHHCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCH
EVILDVVYNHTSEGDSGGPAISWKGIDENLYYFIDKDKNYQDVTGCGNTIAANRGLVRKL
HEEHHHHHCCCCCCCCCCCEEEEECCCCCEEEEEECCCCCCHHHCCCCCHHHCCHHHHHH
IIESLKCWANEFGVDGFRFDLGIALSRGEDLVPLDNPPIFEDIECEPELADIKLISEPWD
HHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCC
CGGLYKLNNFPSKKTFTWNGHFRDDLRRFWRGDKDSAWNMSDKIKGSPSHYKENISFPKS
CCCEEEECCCCCCCEEEECCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHCCCCCCC
INFITSHDGFTLKDLVSFNKKHNFANKEQNRDGDNHNNSWNHGVEGPTSNLFIKKLRKRQ
CCEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
QKNFLLSLLISKGVPMILMGDEIGRSQGGNNNSWCQNNSLGWMNWDESQQDLELLTYLKY
HHHHHHHHHHHCCCCEEEECCHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
LIKIRKKFIKFFNPISPQNRKDIDNLVNYYWHGAKLESPDWSSWSHTIAFSMNKGKNNPL
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEECCCCCCCCCEEEEEEECCCCCCCE
IWAGLNAYTKSIDFHLPESKSKWLKVVDTNESGTIMPIPLEHKSITVENRSSVLIISEEV
EEECCHHHHHCEEEECCCCCCCEEEEEECCCCCCEEEEECCCCEEEEECCCCEEEEEHHH
FGTKNIIF
CCCCCCCC
>Mature Secondary Structure 
PLGSSITSKGVNFSLIATNAEYIEILVFEKEDSVAPKAIFKLDQNHKTGPYWHAEINDL
CCCCCCCCCCCEEEEEEECCCEEEEEEEECCCCCCCHHHHEECCCCCCCCEEECCCCCC
KEGSIYAFRIKQKVNEPNKDYSKTVLIDPCSRGITGWSKYSRKNALNNFDNMDSCLKSVV
CCCCEEEEEEEHHCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHH
CDRKVFNFKNFPRPKHSWEETIIYELHIKAFTQSSNEDESCFKKFLKKIPYLKELGITSI
HHHHHHCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCHHHHHHHHHHHCCCHHHCCCCEE
ELLPVFCFDPNDAPNGLENFWGYSPINWFTPHFQYLSNKSAEQNREEFRKFVEECHKENI
EEEEEEEECCCCCCCHHHHHCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCH
EVILDVVYNHTSEGDSGGPAISWKGIDENLYYFIDKDKNYQDVTGCGNTIAANRGLVRKL
HEEHHHHHCCCCCCCCCCCEEEEECCCCCEEEEEECCCCCCHHHCCCCCHHHCCHHHHHH
IIESLKCWANEFGVDGFRFDLGIALSRGEDLVPLDNPPIFEDIECEPELADIKLISEPWD
HHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCC
CGGLYKLNNFPSKKTFTWNGHFRDDLRRFWRGDKDSAWNMSDKIKGSPSHYKENISFPKS
CCCEEEECCCCCCCEEEECCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHCCCCCCC
INFITSHDGFTLKDLVSFNKKHNFANKEQNRDGDNHNNSWNHGVEGPTSNLFIKKLRKRQ
CCEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
QKNFLLSLLISKGVPMILMGDEIGRSQGGNNNSWCQNNSLGWMNWDESQQDLELLTYLKY
HHHHHHHHHHHCCCCEEEECCHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
LIKIRKKFIKFFNPISPQNRKDIDNLVNYYWHGAKLESPDWSSWSHTIAFSMNKGKNNPL
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEECCCCCCCCCEEEEEEECCCCCCCE
IWAGLNAYTKSIDFHLPESKSKWLKVVDTNESGTIMPIPLEHKSITVENRSSVLIISEEV
EEECCHHHHHCEEEECCCCCCCEEEEEECCCCCCEEEEECCCCEEEEECCCCEEEEEHHH
FGTKNIIF
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]