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The map label for this gene is clpR [H]

Identifier: 123966707

GI number: 123966707

Start: 1311907

End: 1312569

Strand: Direct

Name: clpR [H]

Synonym: P9515_14741

Alternate gene names: 123966707

Gene position: 1311907-1312569 (Clockwise)

Preceding gene: 123966706

Following gene: 123966708

Centisome position: 76.98

GC content: 38.61

Gene sequence:

>663_bases
ATGACTGTATCTGCTCCTTATTATGGCGAAAATACCGCTATGAGGACTCCGCCCCCAGATTTACCTTCTCTTCTTTTAAA
GGAAAGAATTGTCTATCTTGGATTGCCGTTATTTTCAGATGATGACGCTAAAAGGCAATTAGGAATGGATGTTACTGAGC
TCATTATTGCTCAACTCCTTTATCTAGAGTTTGATGATCCTGATAAACCTATCTACTTTTATATCAATTCAACAGGTACT
AGTTGGTACACTGGAGATGCTGTTGGTTTTGAAACAGAAGCTTTCGCCATCTGCGATACTATCAACTACATCAAACCACC
TATTCATACAATTTGTATTGGACAAGCCATGGGTACAGCAGCAGTTATCCTATCTTCTGGGACAAAAGGTCATAGAGCAG
CTCTCCCGCATGCTTCTATTGTTCTCCACCAACCTATTAGTGGCGCAAGAGGGCAAGCAACCGATATCCAGATAAGAGCC
GAAGAAGTGTTAAAAAATAAAAAATCAATGCTTGAGATATTATCACGCAACACTGGGAAAAGTATTGAAGATCTCTCCAA
AGACTCTGACAGGATGAGTTATCTAAATCCTCAAGAAGCTTTAGATTACGGAGTAATCGATAGAATACTTACAAGTCAAA
AAGATTTGCCAAATAAAATTTAA

Upstream 100 bases:

>100_bases
AAACTGATAAATGATCCTGAGTCAAACAAATCTTTTAAATCAACAGCGACACAGGGCTAAATCTAGCTAGAATCATTCCA
GTATTTATTTTGTGGAACAT

Downstream 100 bases:

>100_bases
CTCTTACTTTTAATTAACTATTTTTCTAAAATTATGCCAATAGGAACTCCAAGCGTGCCTTATAGACTTCCAGGAAGTCA
ATATGAAAGATGGGTAGACA

Product: ATP-dependent Clp protease-like protein

Products: NA

Alternate protein names: Endopeptidase Clp-like [H]

Number of amino acids: Translated: 220; Mature: 219

Protein sequence:

>220_residues
MTVSAPYYGENTAMRTPPPDLPSLLLKERIVYLGLPLFSDDDAKRQLGMDVTELIIAQLLYLEFDDPDKPIYFYINSTGT
SWYTGDAVGFETEAFAICDTINYIKPPIHTICIGQAMGTAAVILSSGTKGHRAALPHASIVLHQPISGARGQATDIQIRA
EEVLKNKKSMLEILSRNTGKSIEDLSKDSDRMSYLNPQEALDYGVIDRILTSQKDLPNKI

Sequences:

>Translated_220_residues
MTVSAPYYGENTAMRTPPPDLPSLLLKERIVYLGLPLFSDDDAKRQLGMDVTELIIAQLLYLEFDDPDKPIYFYINSTGT
SWYTGDAVGFETEAFAICDTINYIKPPIHTICIGQAMGTAAVILSSGTKGHRAALPHASIVLHQPISGARGQATDIQIRA
EEVLKNKKSMLEILSRNTGKSIEDLSKDSDRMSYLNPQEALDYGVIDRILTSQKDLPNKI
>Mature_219_residues
TVSAPYYGENTAMRTPPPDLPSLLLKERIVYLGLPLFSDDDAKRQLGMDVTELIIAQLLYLEFDDPDKPIYFYINSTGTS
WYTGDAVGFETEAFAICDTINYIKPPIHTICIGQAMGTAAVILSSGTKGHRAALPHASIVLHQPISGARGQATDIQIRAE
EVLKNKKSMLEILSRNTGKSIEDLSKDSDRMSYLNPQEALDYGVIDRILTSQKDLPNKI

Specific function: Has lost the two conserved residues (Ser and His) proposed to be part of the active site. Therefore it could be inactive [H]

COG id: COG0740

COG function: function code OU; Protease subunit of ATP-dependent Clp proteases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S14 family [H]

Homologues:

Organism=Homo sapiens, GI5174419, Length=192, Percent_Identity=41.6666666666667, Blast_Score=148, Evalue=3e-36,
Organism=Escherichia coli, GI1786641, Length=209, Percent_Identity=39.7129186602871, Blast_Score=160, Evalue=7e-41,
Organism=Caenorhabditis elegans, GI17538017, Length=216, Percent_Identity=37.5, Blast_Score=138, Evalue=2e-33,
Organism=Drosophila melanogaster, GI20129427, Length=205, Percent_Identity=38.5365853658537, Blast_Score=144, Evalue=5e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001907 [H]

Pfam domain/function: PF00574 CLP_protease [H]

EC number: 3.4.21.92

Molecular weight: Translated: 24307; Mature: 24176

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVSAPYYGENTAMRTPPPDLPSLLLKERIVYLGLPLFSDDDAKRQLGMDVTELIIAQLL
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHH
YLEFDDPDKPIYFYINSTGTSWYTGDAVGFETEAFAICDTINYIKPPIHTICIGQAMGTA
HHCCCCCCCCEEEEEECCCCCEEECCCCCCCCHHHHHHHHHHHCCCCHHHEEHHHHHCCE
AVILSSGTKGHRAALPHASIVLHQPISGARGQATDIQIRAEEVLKNKKSMLEILSRNTGK
EEEEECCCCCCEECCCCCEEEEECCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCC
SIEDLSKDSDRMSYLNPQEALDYGVIDRILTSQKDLPNKI
CHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TVSAPYYGENTAMRTPPPDLPSLLLKERIVYLGLPLFSDDDAKRQLGMDVTELIIAQLL
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHH
YLEFDDPDKPIYFYINSTGTSWYTGDAVGFETEAFAICDTINYIKPPIHTICIGQAMGTA
HHCCCCCCCCEEEEEECCCCCEEECCCCCCCCHHHHHHHHHHHCCCCHHHEEHHHHHCCE
AVILSSGTKGHRAALPHASIVLHQPISGARGQATDIQIRAEEVLKNKKSMLEILSRNTGK
EEEEECCCCCCEECCCCCEEEEECCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCC
SIEDLSKDSDRMSYLNPQEALDYGVIDRILTSQKDLPNKI
CHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: ATP imidodiphosphate; dATP degradation, [C]

Metal ions: Ca2+; Mg2+; Mn2+; Zn2+ [C]

Kcat value (1/min): 800 [C]

Specific activity: NA

Km value (mM): 0.21 {ATP}} 1.3 {N-succinyl-Leu-Tyr} [C]

Substrates: NA

Specific reaction: NA

General reaction: Hydrolysis of peptide bonds [C]

Inhibitor: Diisopropyl fluorophosphate ClpAP; Fluorosulfonyl benzoyl adenosine; Kappa-Casein; Mg2+; NEM hydrolysis; Neohydrin; Succinyl -Leu-Tyr4-methyl coumarin7-amide; Xaa-Tyr-Leu-Tyr-Trp [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]