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The map label for this gene is thiE [H]

Identifier: 123966668

GI number: 123966668

Start: 1272654

End: 1273715

Strand: Direct

Name: thiE [H]

Synonym: P9515_14351

Alternate gene names: 123966668

Gene position: 1272654-1273715 (Clockwise)

Preceding gene: 123966667

Following gene: 123966669

Centisome position: 74.68

GC content: 29.94

Gene sequence:

>1062_bases
ATGGAACAACCAAAAATAAATCAACCGGAAGACTTACGAATTTCTCAGATTATTGACGCCAATCTAGATAGAGCAAGAGA
AGGATTAAGAGTCTTAGAGGACTGGGCCAGATTTGGCTTAGGAAATGAAGATTTTGTCATAAGAATAAAAAACCTCCGAC
AAATATTAGGTAAAAATCATTTAGAAATTTACAAAAAATCAAGAAATCATATAGAAGATCAATGTAAAGGGTTATCTCAT
ATTGAACAAATCCACCGGAAAAGTCCCTCTAAAATAATAAGTTCTAATTCTGCTAGGGTTCAAGAGGCTCTTAGAGTTAT
TGAAGAGTTTTCAAGAAACCATAATAATAAACTTTCCAAAATAGCTTCTGATATTAGATATGAAATTTACACTTTAGAAA
TTGAACTATTAAATCTAAACACTCGTAAGAGAGCAGAGTTAATAATTAGAGAAAACAATTTATATTCGATAACAGATCAT
AGAGACAACTTATTACAAATAATTGAAAAAATATTGTTAGGAGGAGTAAAAATTATTCAGCACAGATTTAAAGAAGGTAA
TGATAAAAATCATCTCAAAGAAGCAATTCAAGTAAAGAACCTATGTGAAAAATATAATTCTTTGTTCATCGTTAATGACA
GAGTAGATATAGCAATGGCATCAAATGCAGACGGTGTTCATCTTGGGCAAGAAGACATTGATGTAAAAACAGCAAGAAAA
TTACTAGGCAGTTCTAAAATCATTGGTGTTTCAGCAAATAATTCAACTGATATCAATAAAGCTATAAAAGATGGATGCGA
TTACATTGGTATTGGGCCAGTTTTTCAATCCTTAACAAAAAAGGGAAAAGAACCACTCGGGGTTGAGAAGATTAAAACTT
TAATAAAAGATATAAACATTCCTTGTTTTGCTATAGGAGGTATTAACAAATTAAATATTTCTTGTTTAAAAAGTCATAGA
ATTAGCAAGGTTGCAGTAGTTTCAGGGCTACTAAATTCAGAAGATCCAAAAGAAGAAGCTATTATTATCTTAAAAAAACT
TTCCAATGAAAATTATAGTTAA

Upstream 100 bases:

>100_bases
AATCAAGTTTCAGAATATTGATCAACTCTCTAATCAAATTACTATAGATAGAGAAAATGCTTTAAAAATTCTTAATGAGC
ATAGTAAATAAAATTTTAAT

Downstream 100 bases:

>100_bases
TGGTAAAGAAAAAAATATTGAGATAGGAAATGAAAAGGCTTTACTCTCAAGCGCTTTAAAATTGCTAGGTTATAAACCAA
AGACTATAGTCGTTGAAGTG

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MEQPKINQPEDLRISQIIDANLDRAREGLRVLEDWARFGLGNEDFVIRIKNLRQILGKNHLEIYKKSRNHIEDQCKGLSH
IEQIHRKSPSKIISSNSARVQEALRVIEEFSRNHNNKLSKIASDIRYEIYTLEIELLNLNTRKRAELIIRENNLYSITDH
RDNLLQIIEKILLGGVKIIQHRFKEGNDKNHLKEAIQVKNLCEKYNSLFIVNDRVDIAMASNADGVHLGQEDIDVKTARK
LLGSSKIIGVSANNSTDINKAIKDGCDYIGIGPVFQSLTKKGKEPLGVEKIKTLIKDINIPCFAIGGINKLNISCLKSHR
ISKVAVVSGLLNSEDPKEEAIIILKKLSNENYS

Sequences:

>Translated_353_residues
MEQPKINQPEDLRISQIIDANLDRAREGLRVLEDWARFGLGNEDFVIRIKNLRQILGKNHLEIYKKSRNHIEDQCKGLSH
IEQIHRKSPSKIISSNSARVQEALRVIEEFSRNHNNKLSKIASDIRYEIYTLEIELLNLNTRKRAELIIRENNLYSITDH
RDNLLQIIEKILLGGVKIIQHRFKEGNDKNHLKEAIQVKNLCEKYNSLFIVNDRVDIAMASNADGVHLGQEDIDVKTARK
LLGSSKIIGVSANNSTDINKAIKDGCDYIGIGPVFQSLTKKGKEPLGVEKIKTLIKDINIPCFAIGGINKLNISCLKSHR
ISKVAVVSGLLNSEDPKEEAIIILKKLSNENYS
>Mature_353_residues
MEQPKINQPEDLRISQIIDANLDRAREGLRVLEDWARFGLGNEDFVIRIKNLRQILGKNHLEIYKKSRNHIEDQCKGLSH
IEQIHRKSPSKIISSNSARVQEALRVIEEFSRNHNNKLSKIASDIRYEIYTLEIELLNLNTRKRAELIIRENNLYSITDH
RDNLLQIIEKILLGGVKIIQHRFKEGNDKNHLKEAIQVKNLCEKYNSLFIVNDRVDIAMASNADGVHLGQEDIDVKTARK
LLGSSKIIGVSANNSTDINKAIKDGCDYIGIGPVFQSLTKKGKEPLGVEKIKTLIKDINIPCFAIGGINKLNISCLKSHR
ISKVAVVSGLLNSEDPKEEAIIILKKLSNENYS

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=186, Percent_Identity=29.0322580645161, Blast_Score=91, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6325042, Length=207, Percent_Identity=34.7826086956522, Blast_Score=113, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR016229
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 40021; Mature: 40021

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQPKINQPEDLRISQIIDANLDRAREGLRVLEDWARFGLGNEDFVIRIKNLRQILGKNH
CCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHEEEHHHHHHHHCCCH
LEIYKKSRNHIEDQCKGLSHIEQIHRKSPSKIISSNSARVQEALRVIEEFSRNHNNKLSK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHH
IASDIRYEIYTLEIELLNLNTRKRAELIIRENNLYSITDHRDNLLQIIEKILLGGVKIIQ
HHHHHEEEEEEEEEEEEECCCCCCEEEEEECCCEEEECCCHHHHHHHHHHHHHHHHHHHH
HRFKEGNDKNHLKEAIQVKNLCEKYNSLFIVNDRVDIAMASNADGVHLGQEDIDVKTARK
HHHHCCCCHHHHHHHHHHHHHHHHHCCEEEEECCEEEEEECCCCCCCCCCHHHHHHHHHH
LLGSSKIIGVSANNSTDINKAIKDGCDYIGIGPVFQSLTKKGKEPLGVEKIKTLIKDINI
HHCCCCEEEEECCCCCHHHHHHHCCCCEEEHHHHHHHHHHCCCCCCCHHHHHHHHHHCCC
PCFAIGGINKLNISCLKSHRISKVAVVSGLLNSEDPKEEAIIILKKLSNENYS
CEEEECCCCCCHHHHHHHCCHHHHHHHHHHHCCCCCCHHHEEHHHHHCCCCCC
>Mature Secondary Structure
MEQPKINQPEDLRISQIIDANLDRAREGLRVLEDWARFGLGNEDFVIRIKNLRQILGKNH
CCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHEEEHHHHHHHHCCCH
LEIYKKSRNHIEDQCKGLSHIEQIHRKSPSKIISSNSARVQEALRVIEEFSRNHNNKLSK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHH
IASDIRYEIYTLEIELLNLNTRKRAELIIRENNLYSITDHRDNLLQIIEKILLGGVKIIQ
HHHHHEEEEEEEEEEEEECCCCCCEEEEEECCCEEEECCCHHHHHHHHHHHHHHHHHHHH
HRFKEGNDKNHLKEAIQVKNLCEKYNSLFIVNDRVDIAMASNADGVHLGQEDIDVKTARK
HHHHCCCCHHHHHHHHHHHHHHHHHCCEEEEECCEEEEEECCCCCCCCCCHHHHHHHHHH
LLGSSKIIGVSANNSTDINKAIKDGCDYIGIGPVFQSLTKKGKEPLGVEKIKTLIKDINI
HHCCCCEEEEECCCCCHHHHHHHCCCCEEEHHHHHHHHHHCCCCCCCHHHHHHHHHHCCC
PCFAIGGINKLNISCLKSHRISKVAVVSGLLNSEDPKEEAIIILKKLSNENYS
CEEEECCCCCCHHHHHHHCCHHHHHHHHHHHCCCCCCHHHEEHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]