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The map label for this gene is pheS [H]

Identifier: 123966664

GI number: 123966664

Start: 1269255

End: 1270262

Strand: Reverse

Name: pheS [H]

Synonym: P9515_14311

Alternate gene names: 123966664

Gene position: 1270262-1269255 (Counterclockwise)

Preceding gene: 123966666

Following gene: 123966663

Centisome position: 74.54

GC content: 34.33

Gene sequence:

>1008_bases
GTGAGTCAAATTGAATCATTAAGCCAAATAGAGGAAAAGTTAAATAATCTTTCTTTATTGGCCAATGAAAACATTAAAAA
TGCTAATACTGATGCTGAATTGGATCAACTACGAGTTTCTTTACTAGGAAAGAAAGGTGAGTTATCAATAATATTAAAAA
CTATGGGAAAATTGTCCTCAGTTGATAGACCTATTGTTGGGCAGAAGGCCAACTTGATAAAGATAAATTTGCAAGATTTA
ATAACTGAAAGAAAAGATCAACTTAATAGTGACGCTATAGATCAACAAATTAAAGATGAAAAAATTGATGTAACTATACC
TTCCACAGGAACCCACTCAGGTCATAAACACCCTTTGATTTCCACACAAGATGAGATTATAGATATTTTTTGCGGACTAG
GTTACTCCGTTGAAAATGGTCCTGAAATAGAGAGTGATTTTTATAATTTCGAGTCGCTCAACATACCTAAAAATCATCCT
GCAAGAGATATGCAGGATACTTTTTATTTAGATGAAAATCGTTTACTCAGAACTCACACCTCTCCTGTTCAGATTAGATA
TCTTGAAAAAAATCCGCCTCCAGTAAGAATTATTGCACCTGGGAGAGTTTATAGGAGAGATGCGGTCGACGCTACTCACT
CCCCTGTTTTTAATCAAGTAGAGGTTTTATGTCTAGATCAAGATATAAATTTTAGTCATTTAAGAGGAACTGTACTTACT
TTTTTAAAAACTTTCTTTGGGGACATTCCAGTTAGATTTAGGGCTAGTTATTTTCCTTTTACTGAACCCTCAGCAGAAGT
GGATGTTCAATGGAAAGGTAAATGGCTGGAAGTAATGGGTTGTGGAATGGTAGATCCAAAGGTTTTAGAAAAATTAGGAA
TTGACTCTGAAAGATATACTGGTTTTGCCGCTGGATTAGGTGTAGAAAGATTTTGTATGGTGAGACATCAGATTGATGAC
ATAAGGAGGTTTTACACTAATGATCTAAGATTCCTAGAACAGTTTTAA

Upstream 100 bases:

>100_bases
ATCATTACTTATTAATATATTTAACGATTCCATAGGTTATTTCATTTTATTATGGACGATATTTAAGTAAGATAAGAAAA
TACTTATTTTTACTATATCA

Downstream 100 bases:

>100_bases
AATAATTAGTGTTTAAATTAGGTTTCTTAATCAATTTAGATTAAAATATTAGTAGTTTGTAATTTATTGATTGGTACGTA
AAGCTGGACTAATCGTCAAT

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS [H]

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MSQIESLSQIEEKLNNLSLLANENIKNANTDAELDQLRVSLLGKKGELSIILKTMGKLSSVDRPIVGQKANLIKINLQDL
ITERKDQLNSDAIDQQIKDEKIDVTIPSTGTHSGHKHPLISTQDEIIDIFCGLGYSVENGPEIESDFYNFESLNIPKNHP
ARDMQDTFYLDENRLLRTHTSPVQIRYLEKNPPPVRIIAPGRVYRRDAVDATHSPVFNQVEVLCLDQDINFSHLRGTVLT
FLKTFFGDIPVRFRASYFPFTEPSAEVDVQWKGKWLEVMGCGMVDPKVLEKLGIDSERYTGFAAGLGVERFCMVRHQIDD
IRRFYTNDLRFLEQF

Sequences:

>Translated_335_residues
MSQIESLSQIEEKLNNLSLLANENIKNANTDAELDQLRVSLLGKKGELSIILKTMGKLSSVDRPIVGQKANLIKINLQDL
ITERKDQLNSDAIDQQIKDEKIDVTIPSTGTHSGHKHPLISTQDEIIDIFCGLGYSVENGPEIESDFYNFESLNIPKNHP
ARDMQDTFYLDENRLLRTHTSPVQIRYLEKNPPPVRIIAPGRVYRRDAVDATHSPVFNQVEVLCLDQDINFSHLRGTVLT
FLKTFFGDIPVRFRASYFPFTEPSAEVDVQWKGKWLEVMGCGMVDPKVLEKLGIDSERYTGFAAGLGVERFCMVRHQIDD
IRRFYTNDLRFLEQF
>Mature_334_residues
SQIESLSQIEEKLNNLSLLANENIKNANTDAELDQLRVSLLGKKGELSIILKTMGKLSSVDRPIVGQKANLIKINLQDLI
TERKDQLNSDAIDQQIKDEKIDVTIPSTGTHSGHKHPLISTQDEIIDIFCGLGYSVENGPEIESDFYNFESLNIPKNHPA
RDMQDTFYLDENRLLRTHTSPVQIRYLEKNPPPVRIIAPGRVYRRDAVDATHSPVFNQVEVLCLDQDINFSHLRGTVLTF
LKTFFGDIPVRFRASYFPFTEPSAEVDVQWKGKWLEVMGCGMVDPKVLEKLGIDSERYTGFAAGLGVERFCMVRHQIDDI
RRFYTNDLRFLEQF

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5729820, Length=269, Percent_Identity=30.8550185873606, Blast_Score=135, Evalue=4e-32,
Organism=Homo sapiens, GI4758340, Length=257, Percent_Identity=33.852140077821, Blast_Score=130, Evalue=2e-30,
Organism=Escherichia coli, GI1788007, Length=324, Percent_Identity=50.6172839506173, Blast_Score=328, Evalue=3e-91,
Organism=Caenorhabditis elegans, GI32563657, Length=285, Percent_Identity=29.8245614035088, Blast_Score=131, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI17508957, Length=285, Percent_Identity=29.8245614035088, Blast_Score=131, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI32566635, Length=241, Percent_Identity=34.8547717842324, Blast_Score=130, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6321087, Length=262, Percent_Identity=31.6793893129771, Blast_Score=139, Evalue=9e-34,
Organism=Saccharomyces cerevisiae, GI6325304, Length=88, Percent_Identity=42.0454545454545, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI17137424, Length=278, Percent_Identity=29.8561151079137, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI18858079, Length=271, Percent_Identity=29.8892988929889, Blast_Score=117, Evalue=7e-27,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978 [H]

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 38214; Mature: 38083

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQIESLSQIEEKLNNLSLLANENIKNANTDAELDQLRVSLLGKKGELSIILKTMGKLSS
CCHHHHHHHHHHHHHCEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
VDRPIVGQKANLIKINLQDLITERKDQLNSDAIDQQIKDEKIDVTIPSTGTHSGHKHPLI
CCCCCCCCCCCEEEEEHHHHHHHHHHHCCHHHHHHHHCCCEEEEEECCCCCCCCCCCCCC
STQDEIIDIFCGLGYSVENGPEIESDFYNFESLNIPKNHPARDMQDTFYLDENRLLRTHT
CCHHHHHHHHHCCCCCCCCCCCCCHHHCCCEECCCCCCCCCCCCCHHEEECCCCEEEECC
SPVQIRYLEKNPPPVRIIAPGRVYRRDAVDATHSPVFNQVEVLCLDQDINFSHLRGTVLT
CCEEEEEEECCCCCEEEECCCCHHHHHCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHH
FLKTFFGDIPVRFRASYFPFTEPSAEVDVQWKGKWLEVMGCGMVDPKVLEKLGIDSERYT
HHHHHHCCCCEEEEECCCCCCCCCCCEEEEECCCEEEEECCCCCCHHHHHHHCCCCHHCC
GFAAGLGVERFCMVRHQIDDIRRFYTNDLRFLEQF
CHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SQIESLSQIEEKLNNLSLLANENIKNANTDAELDQLRVSLLGKKGELSIILKTMGKLSS
CHHHHHHHHHHHHHCEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
VDRPIVGQKANLIKINLQDLITERKDQLNSDAIDQQIKDEKIDVTIPSTGTHSGHKHPLI
CCCCCCCCCCCEEEEEHHHHHHHHHHHCCHHHHHHHHCCCEEEEEECCCCCCCCCCCCCC
STQDEIIDIFCGLGYSVENGPEIESDFYNFESLNIPKNHPARDMQDTFYLDENRLLRTHT
CCHHHHHHHHHCCCCCCCCCCCCCHHHCCCEECCCCCCCCCCCCCHHEEECCCCEEEECC
SPVQIRYLEKNPPPVRIIAPGRVYRRDAVDATHSPVFNQVEVLCLDQDINFSHLRGTVLT
CCEEEEEEECCCCCEEEECCCCHHHHHCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHH
FLKTFFGDIPVRFRASYFPFTEPSAEVDVQWKGKWLEVMGCGMVDPKVLEKLGIDSERYT
HHHHHHCCCCEEEEECCCCCCCCCCCEEEEECCCEEEEECCCCCCHHHHHHHCCCCHHCC
GFAAGLGVERFCMVRHQIDDIRRFYTNDLRFLEQF
CHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]