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The map label for this gene is sppA [C]

Identifier: 123966585

GI number: 123966585

Start: 1186675

End: 1187484

Strand: Direct

Name: sppA [C]

Synonym: P9515_13521

Alternate gene names: 123966585

Gene position: 1186675-1187484 (Clockwise)

Preceding gene: 123966581

Following gene: 123966586

Centisome position: 69.63

GC content: 33.33

Gene sequence:

>810_bases
ATGATTTGGCCTTTTAGACGAAAGTCAAAAAAAAGAATGGCTCGCATAGTAATTGATGAGCCAATCACAAGTTCAACCAG
AGTTTCTGTCATCAAAGCTTTAAAACAGATTGAAGATAGAGAATTTCCAGCATTAATTCTAAGAATTGACTCTCCAGGAG
GTACCGTTGGAGATAGCCAAGAAATATACTCTGCTATTAAACGTCTAAGAGAGAAAGGATGTAAGGTAGTTGCTAGTTTT
GGAAACATATCTGCTTCTGGTGGTGTTTACATCGGAGTTGCTTCAAACAAAATTGTAGCTAACCCAGGTACAATTACGGG
CTCAATTGGTGTAATAATAAGAGGAAATAATTTATCTGAACTATTGAATAAAATAGGTATCAAGTTTGAAACTGTAAAAA
GTGGTATCTATAAAGATATTCTTTCGCCAGATAAACCTTTAAGTGATGAAGGAAGAGAAATCTTACAAGGATTAATAGAT
GAAAGTTACAAACAATTTACTGAGGCCGTAGCAGAAGGAAGGGATTTACCGGTAGAAGATGTAAAGAAATTTGCCGATGG
AAGAATCTTTACTGGAACTCAAGCTAAAAAACTTGGTTTAGTTGATGAAATTGGTGATGAATTTACCGCGAGAGAGCTTG
CAGCAGAAATGGTGAAAATCGATCCCAAAATACAACCTGTAACTTTTGGTAAGAAAAAAAAGAAAATACTAGGACTTATC
CCTGGTAGTAAAATTATTGAGAAAATTATTCAAAATATATTTTTTGAAGTAAATTCAACTAATAAAATTCTTTGGTTATA
TAAACCTTAA

Upstream 100 bases:

>100_bases
AGTGCAAAAGGGAGTATCATTAAAAACCAATTTAGGATTGAATTCATTATGTCTGCTGATAGTCTTTTTAGAGTAGATTT
ATGATTTGTACCTTAAAAAC

Downstream 100 bases:

>100_bases
TTTAAATATGAATAAGGATTATCTTGACAGTGAAAATATTACTGCTCTCAGAGGAGCAACAACTTCAAGTGGAAATTCGA
TAAAAGAGATTGAAAACGCA

Product: signal peptide peptidase SppA (protease IV)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MIWPFRRKSKKRMARIVIDEPITSSTRVSVIKALKQIEDREFPALILRIDSPGGTVGDSQEIYSAIKRLREKGCKVVASF
GNISASGGVYIGVASNKIVANPGTITGSIGVIIRGNNLSELLNKIGIKFETVKSGIYKDILSPDKPLSDEGREILQGLID
ESYKQFTEAVAEGRDLPVEDVKKFADGRIFTGTQAKKLGLVDEIGDEFTARELAAEMVKIDPKIQPVTFGKKKKKILGLI
PGSKIIEKIIQNIFFEVNSTNKILWLYKP

Sequences:

>Translated_269_residues
MIWPFRRKSKKRMARIVIDEPITSSTRVSVIKALKQIEDREFPALILRIDSPGGTVGDSQEIYSAIKRLREKGCKVVASF
GNISASGGVYIGVASNKIVANPGTITGSIGVIIRGNNLSELLNKIGIKFETVKSGIYKDILSPDKPLSDEGREILQGLID
ESYKQFTEAVAEGRDLPVEDVKKFADGRIFTGTQAKKLGLVDEIGDEFTARELAAEMVKIDPKIQPVTFGKKKKKILGLI
PGSKIIEKIIQNIFFEVNSTNKILWLYKP
>Mature_269_residues
MIWPFRRKSKKRMARIVIDEPITSSTRVSVIKALKQIEDREFPALILRIDSPGGTVGDSQEIYSAIKRLREKGCKVVASF
GNISASGGVYIGVASNKIVANPGTITGSIGVIIRGNNLSELLNKIGIKFETVKSGIYKDILSPDKPLSDEGREILQGLID
ESYKQFTEAVAEGRDLPVEDVKKFADGRIFTGTQAKKLGLVDEIGDEFTARELAAEMVKIDPKIQPVTFGKKKKKILGLI
PGSKIIEKIIQNIFFEVNSTNKILWLYKP

Specific function: Digestion Of The Cleaved Signal Peptides. This Activity Is Necessary To Maintain Proper Secretion Of Mature Proteins Across The Membrane. [C]

COG id: COG0616

COG function: function code OU; Periplasmic serine proteases (ClpP class)

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S49 family [H]

Homologues:

Organism=Escherichia coli, GI1788064, Length=182, Percent_Identity=37.9120879120879, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1787527, Length=160, Percent_Identity=34.375, Blast_Score=93, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004635
- InterPro:   IPR002142 [H]

Pfam domain/function: PF01343 Peptidase_S49 [H]

EC number: 3.4.21.-

Molecular weight: Translated: 29706; Mature: 29706

Theoretical pI: Translated: 10.21; Mature: 10.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIWPFRRKSKKRMARIVIDEPITSSTRVSVIKALKQIEDREFPALILRIDSPGGTVGDSQ
CCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHH
EIYSAIKRLREKGCKVVASFGNISASGGVYIGVASNKIVANPGTITGSIGVIIRGNNLSE
HHHHHHHHHHHCCCEEEECCCCCCCCCCEEEEEECCEEEECCCCEEECEEEEEECCCHHH
LLNKIGIKFETVKSGIYKDILSPDKPLSDEGREILQGLIDESYKQFTEAVAEGRDLPVED
HHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
VKKFADGRIFTGTQAKKLGLVDEIGDEFTARELAAEMVKIDPKIQPVTFGKKKKKILGLI
HHHHHCCCEEECCCHHHCCCHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHEEEEC
PGSKIIEKIIQNIFFEVNSTNKILWLYKP
CCHHHHHHHHHHHHEEECCCCEEEEEECC
>Mature Secondary Structure
MIWPFRRKSKKRMARIVIDEPITSSTRVSVIKALKQIEDREFPALILRIDSPGGTVGDSQ
CCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHH
EIYSAIKRLREKGCKVVASFGNISASGGVYIGVASNKIVANPGTITGSIGVIIRGNNLSE
HHHHHHHHHHHCCCEEEECCCCCCCCCCEEEEEECCEEEECCCCEEECEEEEEECCCHHH
LLNKIGIKFETVKSGIYKDILSPDKPLSDEGREILQGLIDESYKQFTEAVAEGRDLPVED
HHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
VKKFADGRIFTGTQAKKLGLVDEIGDEFTARELAAEMVKIDPKIQPVTFGKKKKKILGLI
HHHHHCCCEEECCCHHHCCCHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHEEEEC
PGSKIIEKIIQNIFFEVNSTNKILWLYKP
CCHHHHHHHHHHHHEEECCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]