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The map label for this gene is ligA [H]

Identifier: 123966480

GI number: 123966480

Start: 1097353

End: 1098108

Strand: Reverse

Name: ligA [H]

Synonym: P9515_12471

Alternate gene names: 123966480

Gene position: 1098108-1097353 (Counterclockwise)

Preceding gene: 123966483

Following gene: 123966479

Centisome position: 64.44

GC content: 29.89

Gene sequence:

>756_bases
ATGACAATTTATTTAGAAGAAAGAATTGAATGGTATGACCATAATTATAGAATGGGAACTCCTTTAATTAGTGATGTCCA
ATTCGATCAATTAGAAGCTAATTTATATCGAGTAAATCCAAAAGCTAATTACTTTCATAAAAAATCAATATTACCTTTAC
CATCATTGCCAAAAGATAAAATTTCAGAATTTTTAGAAGGATTATTGCCAAATACAAGATTAATTATTGAACCTAAAATT
GATGGTTGCGCAATCGGTATTCAATATATTGATGGCCAATTAATAAAAGCAATTTCAAGAAAAGGTGATGATGTTACTAA
CAAAATAAAAGAGATTCTTGATGTTCCAAATGAGATAAAAGTTAAAGGTTTAATCCAAGTGAGAGGAGAGCTCTTTGCCC
CATTGGAAAATGAAAGGCCAACTTATTCTCAAAGAAAGGCAGGTGGTTATCTTCGGAATGAAGCCAGTAAAAACGATCAT
CTCAGCTTTTGTGGCTTTCAGATAATTAATGGCAGATTAAATGAACATGATTCTTTGGTGTATTTGAAGAAGTTGGGTTT
TACAATTCCAGAATATAAGAGTTTAAAGTATTTAACTCAAGTTGAAATGTATAGAAAACAATGGGCTGAAAAAAAACTAT
TTACTCAATATCCAACAGATGGAATAGTAGTTAAACTAAATTCCAGAAAATTACAATTAATTAGAGAAAAATCTGTTGGT
TGCTATCCTTACTGGCAGATGGCAATAAATTTTTAA

Upstream 100 bases:

>100_bases
AATTCCTTATATTAAGTTCATCTTTAAGAGGAAATAATTAATTGACAGTCGATCTAAAATAAGAGGTAATTAGATTCTTT
TTTAAAGTGCTAAGAAATAT

Downstream 100 bases:

>100_bases
TTATGCAAGAATTACAGGAAAACACAATTGATCAAATAAGAACTCTTCTTAGTTATTTGAAGCAAACAGATCTTTTAAAA
GATAAAGATATTCTTAGGTG

Product: NAD-dependent DNA ligase N-terminus

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] [H]

Number of amino acids: Translated: 251; Mature: 250

Protein sequence:

>251_residues
MTIYLEERIEWYDHNYRMGTPLISDVQFDQLEANLYRVNPKANYFHKKSILPLPSLPKDKISEFLEGLLPNTRLIIEPKI
DGCAIGIQYIDGQLIKAISRKGDDVTNKIKEILDVPNEIKVKGLIQVRGELFAPLENERPTYSQRKAGGYLRNEASKNDH
LSFCGFQIINGRLNEHDSLVYLKKLGFTIPEYKSLKYLTQVEMYRKQWAEKKLFTQYPTDGIVVKLNSRKLQLIREKSVG
CYPYWQMAINF

Sequences:

>Translated_251_residues
MTIYLEERIEWYDHNYRMGTPLISDVQFDQLEANLYRVNPKANYFHKKSILPLPSLPKDKISEFLEGLLPNTRLIIEPKI
DGCAIGIQYIDGQLIKAISRKGDDVTNKIKEILDVPNEIKVKGLIQVRGELFAPLENERPTYSQRKAGGYLRNEASKNDH
LSFCGFQIINGRLNEHDSLVYLKKLGFTIPEYKSLKYLTQVEMYRKQWAEKKLFTQYPTDGIVVKLNSRKLQLIREKSVG
CYPYWQMAINF
>Mature_250_residues
TIYLEERIEWYDHNYRMGTPLISDVQFDQLEANLYRVNPKANYFHKKSILPLPSLPKDKISEFLEGLLPNTRLIIEPKID
GCAIGIQYIDGQLIKAISRKGDDVTNKIKEILDVPNEIKVKGLIQVRGELFAPLENERPTYSQRKAGGYLRNEASKNDHL
SFCGFQIINGRLNEHDSLVYLKKLGFTIPEYKSLKYLTQVEMYRKQWAEKKLFTQYPTDGIVVKLNSRKLQLIREKSVGC
YPYWQMAINF

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149 [H]

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 29253; Mature: 29122

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: PS00589 PTS_HPR_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIYLEERIEWYDHNYRMGTPLISDVQFDQLEANLYRVNPKANYFHKKSILPLPSLPKDK
CEEEEECHHHHHCCCEEECCCCCCCCCHHHCCCEEEEECCCCCCEECCCCCCCCCCCHHH
ISEFLEGLLPNTRLIIEPKIDGCAIGIQYIDGQLIKAISRKGDDVTNKIKEILDVPNEIK
HHHHHHHHCCCCEEEEEECCCCEEEEEEHHCHHHHHHHHHCCCHHHHHHHHHHCCCCHHE
VKGLIQVRGELFAPLENERPTYSQRKAGGYLRNEASKNDHLSFCGFQIINGRLNEHDSLV
EEHHEEEHHHHCCCCCCCCCCCHHHHCCCCHHCCCCCCCCEEEEEEEEECCCCCCCCCEE
YLKKLGFTIPEYKSLKYLTQVEMYRKQWAEKKLFTQYPTDGIVVKLNSRKLQLIREKSVG
EEHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEHHHHCCCC
CYPYWQMAINF
CCEEEEEEECC
>Mature Secondary Structure 
TIYLEERIEWYDHNYRMGTPLISDVQFDQLEANLYRVNPKANYFHKKSILPLPSLPKDK
EEEEECHHHHHCCCEEECCCCCCCCCHHHCCCEEEEECCCCCCEECCCCCCCCCCCHHH
ISEFLEGLLPNTRLIIEPKIDGCAIGIQYIDGQLIKAISRKGDDVTNKIKEILDVPNEIK
HHHHHHHHCCCCEEEEEECCCCEEEEEEHHCHHHHHHHHHCCCHHHHHHHHHHCCCCHHE
VKGLIQVRGELFAPLENERPTYSQRKAGGYLRNEASKNDHLSFCGFQIINGRLNEHDSLV
EEHHEEEHHHHCCCCCCCCCCCHHHHCCCCHHCCCCCCCCEEEEEEEEECCCCCCCCCEE
YLKKLGFTIPEYKSLKYLTQVEMYRKQWAEKKLFTQYPTDGIVVKLNSRKLQLIREKSVG
EEHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEHHHHCCCC
CYPYWQMAINF
CCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA