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The map label for this gene is rsmD [H]

Identifier: 123966382

GI number: 123966382

Start: 1000450

End: 1001037

Strand: Reverse

Name: rsmD [H]

Synonym: P9515_11491

Alternate gene names: 123966382

Gene position: 1001037-1000450 (Counterclockwise)

Preceding gene: 123966383

Following gene: 123966380

Centisome position: 58.74

GC content: 26.36

Gene sequence:

>588_bases
ATGAAATCAAATTTAAGGTTAATTGGAGGAAGAAAGCTTGAGAGCCCAAATACTAATGAATCTAGGCCAACTACTTTAAT
GGTTAGAGAAGCTATTTTTAATATTTTGAAAAATACAGTTGAAAATTCTAATTGGTTGGATTTATTTAGTGGGACAGGAT
CAATATCCTGTGAGGCATATAATCATGGTGCAAAAAAAATAATTGCAGTTGAAAAAAATAAAAATCTCTCAAAAATATGT
TTAAAGAATTTATATTCATTGCAAAATATGGATAATAGAAAAAATGATATTGAGGTTATTTGCAAAGATGTATTGATTTG
GACAAAACCCAGTGCCTCCAAAAATTATTCTACTAAGAATAATTTAAATAAAATAAAATTTGACTTTATATATTTAGATC
CCCCTTATAGAGCTGATTATCATGAACTGGTTTTAAATCAAATATTTAATTGCAACTTTATAAGAAAAGATACGATAGTT
ATCTGCGAACATTCATTAGATTTGGAGATAAAGAAGAATATTTTATGGGAGATTTATGATGTTAGAACTTATGGTCAATC
AAAATTGACTTTTTTAATCCATATCTAA

Upstream 100 bases:

>100_bases
AATTTACTTGCTTGTCAGTTTCATCCTGAAAAGTCAGGAAAAACAGGAGAAAAACTTTTACGTAGATGGCTTAAAAATAT
TCAATAATTCTATGTGATAT

Downstream 100 bases:

>100_bases
CAAACCTGACCCTCTTCTATATTGATTCCATGCGCTTACAAATAATCCTAGAAGTGTTATTGGAACTAATCCTAAAACTA
TTCCACATAAAAGAGGTTCA

Product: N6-adenine-specific methylase

Products: NA

Alternate protein names: 16S rRNA m2G966 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase [H]

Number of amino acids: Translated: 195; Mature: 195

Protein sequence:

>195_residues
MKSNLRLIGGRKLESPNTNESRPTTLMVREAIFNILKNTVENSNWLDLFSGTGSISCEAYNHGAKKIIAVEKNKNLSKIC
LKNLYSLQNMDNRKNDIEVICKDVLIWTKPSASKNYSTKNNLNKIKFDFIYLDPPYRADYHELVLNQIFNCNFIRKDTIV
ICEHSLDLEIKKNILWEIYDVRTYGQSKLTFLIHI

Sequences:

>Translated_195_residues
MKSNLRLIGGRKLESPNTNESRPTTLMVREAIFNILKNTVENSNWLDLFSGTGSISCEAYNHGAKKIIAVEKNKNLSKIC
LKNLYSLQNMDNRKNDIEVICKDVLIWTKPSASKNYSTKNNLNKIKFDFIYLDPPYRADYHELVLNQIFNCNFIRKDTIV
ICEHSLDLEIKKNILWEIYDVRTYGQSKLTFLIHI
>Mature_195_residues
MKSNLRLIGGRKLESPNTNESRPTTLMVREAIFNILKNTVENSNWLDLFSGTGSISCEAYNHGAKKIIAVEKNKNLSKIC
LKNLYSLQNMDNRKNDIEVICKDVLIWTKPSASKNYSTKNNLNKIKFDFIYLDPPYRADYHELVLNQIFNCNFIRKDTIV
ICEHSLDLEIKKNILWEIYDVRTYGQSKLTFLIHI

Specific function: Specifically methylates the guanosine in position 966 of 16S rRNA in the assembled 30S particle [H]

COG id: COG0742

COG function: function code L; N6-adenine-specific methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmD family [H]

Homologues:

Organism=Escherichia coli, GI1789875, Length=191, Percent_Identity=25.130890052356, Blast_Score=68, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004398 [H]

Pfam domain/function: PF03602 Cons_hypoth95 [H]

EC number: =2.1.1.171 [H]

Molecular weight: Translated: 22679; Mature: 22679

Theoretical pI: Translated: 9.33; Mature: 9.33

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSNLRLIGGRKLESPNTNESRPTTLMVREAIFNILKNTVENSNWLDLFSGTGSISCEAY
CCCCEEEECCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEE
NHGAKKIIAVEKNKNLSKICLKNLYSLQNMDNRKNDIEVICKDVLIWTKPSASKNYSTKN
CCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCHHHHHEEHHEEEECCCCCCCCCCCC
NLNKIKFDFIYLDPPYRADYHELVLNQIFNCNFIRKDTIVICEHSLDLEIKKNILWEIYD
CCCEEEEEEEEECCCCCCCHHHHHHHHHHCCCEEECCEEEEEECCCCEEEECCEEEEEEE
VRTYGQSKLTFLIHI
EEECCCCEEEEEEEC
>Mature Secondary Structure
MKSNLRLIGGRKLESPNTNESRPTTLMVREAIFNILKNTVENSNWLDLFSGTGSISCEAY
CCCCEEEECCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEE
NHGAKKIIAVEKNKNLSKICLKNLYSLQNMDNRKNDIEVICKDVLIWTKPSASKNYSTKN
CCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCHHHHHEEHHEEEECCCCCCCCCCCC
NLNKIKFDFIYLDPPYRADYHELVLNQIFNCNFIRKDTIVICEHSLDLEIKKNILWEIYD
CCCEEEEEEEEECCCCCCCHHHHHHHHHHCCCEEECCEEEEEECCCCEEEECCEEEEEEE
VRTYGQSKLTFLIHI
EEECCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]