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The map label for this gene is lasT [C]

Identifier: 123966380

GI number: 123966380

Start: 999159

End: 999869

Strand: Reverse

Name: lasT [C]

Synonym: P9515_11471

Alternate gene names: 123966380

Gene position: 999869-999159 (Counterclockwise)

Preceding gene: 123966382

Following gene: 123966379

Centisome position: 58.67

GC content: 27.85

Gene sequence:

>711_bases
TTGGTTGAACCAAATGGGCCAATAAATATTGGCAGTATCGCTAGACTATGTTCTAATTTTGATGTGAATGAATTAAGGAT
TGTTTCACCTAAATGTGATATTTTTTCTTTGGAAGCAAAAAAAATGGCCTTAAAGGGTCAAAAATATATAAATAATTGTA
GAGTTTTTAATAGTATTGAACAAGCAATATTTGATTGTGATTTAGTTCTTGCTACTTGTGGAAGGATTGATTTAAGTAAT
GATATTGAGTGTGAAAATCCTGAGGTGATTTCTAAATGGATTTCATCTTTTAAGGTTATTAATAATTTGGCTATTTTATT
TGGAAGAGAAGATAGAGGTTTAACTAATAGTGAATTACTTTTTGCTCACAAGATTTTTAACATAAAAACTTCCCAAGACT
ATCCCTCTTTAAACCTTTCTCACGCGGTTGCAATAATTTTGTATGAATTAAACAAATGTTCAAAAAATACTCCTCAGAAA
GATTTAAAAGTTTTTAACCTTGCATCATCAAAGCAAATTTACGAATCATTTAAAGATTTAGAAGAAATACTTATTAGGAC
CGGTTATCTTTTAGAACATACATCCTTTTCAAAAATAAGTAAGTTTAAAAAATTTATTTTAAAAGCGAAAACATCTAGTC
ATGAGATAAATGTCTTAAGAGGAATTGTTCGTCAAATCAAATGGTTCTTAAATAATTCAAAAGGCAATTGA

Upstream 100 bases:

>100_bases
AAGTTTGAAACTGTTCCTCAAAATATTTCCAAATTTAGAACATCTCCACATAGCAAAAAAATAAATTTTGAAGAAAAATG
TTGTTAACTTGAAGGTTGTT

Downstream 100 bases:

>100_bases
AATTAACTTTGTTAGATAAAAATTCAAGCAAATATTTGTTCTTGATTTTATGGCTGAATTTATTTTAAAAAGTATATATT
CAGTTTAAAAAATTAGAATT

Product: tRNA/rRNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MVEPNGPINIGSIARLCSNFDVNELRIVSPKCDIFSLEAKKMALKGQKYINNCRVFNSIEQAIFDCDLVLATCGRIDLSN
DIECENPEVISKWISSFKVINNLAILFGREDRGLTNSELLFAHKIFNIKTSQDYPSLNLSHAVAIILYELNKCSKNTPQK
DLKVFNLASSKQIYESFKDLEEILIRTGYLLEHTSFSKISKFKKFILKAKTSSHEINVLRGIVRQIKWFLNNSKGN

Sequences:

>Translated_236_residues
MVEPNGPINIGSIARLCSNFDVNELRIVSPKCDIFSLEAKKMALKGQKYINNCRVFNSIEQAIFDCDLVLATCGRIDLSN
DIECENPEVISKWISSFKVINNLAILFGREDRGLTNSELLFAHKIFNIKTSQDYPSLNLSHAVAIILYELNKCSKNTPQK
DLKVFNLASSKQIYESFKDLEEILIRTGYLLEHTSFSKISKFKKFILKAKTSSHEINVLRGIVRQIKWFLNNSKGN
>Mature_236_residues
MVEPNGPINIGSIARLCSNFDVNELRIVSPKCDIFSLEAKKMALKGQKYINNCRVFNSIEQAIFDCDLVLATCGRIDLSN
DIECENPEVISKWISSFKVINNLAILFGREDRGLTNSELLFAHKIFNIKTSQDYPSLNLSHAVAIILYELNKCSKNTPQK
DLKVFNLASSKQIYESFKDLEEILIRTGYLLEHTSFSKISKFKKFILKAKTSSHEINVLRGIVRQIKWFLNNSKGN

Specific function: Unknown

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=247, Percent_Identity=27.9352226720648, Blast_Score=94, Evalue=8e-21,
Organism=Escherichia coli, GI1790865, Length=167, Percent_Identity=29.940119760479, Blast_Score=80, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26856; Mature: 26856

Theoretical pI: Translated: 9.05; Mature: 9.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEPNGPINIGSIARLCSNFDVNELRIVSPKCDIFSLEAKKMALKGQKYINNCRVFNSIE
CCCCCCCCCHHHHHHHHCCCCCCCEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHH
QAIFDCDLVLATCGRIDLSNDIECENPEVISKWISSFKVINNLAILFGREDRGLTNSELL
HHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCEE
FAHKIFNIKTSQDYPSLNLSHAVAIILYELNKCSKNTPQKDLKVFNLASSKQIYESFKDL
EEHHHEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCHHHHHHHHHHHHH
EEILIRTGYLLEHTSFSKISKFKKFILKAKTSSHEINVLRGIVRQIKWFLNNSKGN
HHHHHHHCHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MVEPNGPINIGSIARLCSNFDVNELRIVSPKCDIFSLEAKKMALKGQKYINNCRVFNSIE
CCCCCCCCCHHHHHHHHCCCCCCCEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHH
QAIFDCDLVLATCGRIDLSNDIECENPEVISKWISSFKVINNLAILFGREDRGLTNSELL
HHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCEE
FAHKIFNIKTSQDYPSLNLSHAVAIILYELNKCSKNTPQKDLKVFNLASSKQIYESFKDL
EEHHHEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCHHHHHHHHHHHHH
EEILIRTGYLLEHTSFSKISKFKKFILKAKTSSHEINVLRGIVRQIKWFLNNSKGN
HHHHHHHCHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]