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The map label for this gene is dus1 [H]

Identifier: 123966327

GI number: 123966327

Start: 964448

End: 965455

Strand: Direct

Name: dus1 [H]

Synonym: P9515_10941

Alternate gene names: 123966327

Gene position: 964448-965455 (Clockwise)

Preceding gene: 123966324

Following gene: 123966328

Centisome position: 56.59

GC content: 32.54

Gene sequence:

>1008_bases
ATGTCCTCAGTTATAAAATTAAGAGGAAAAGGTTTATCTAGAATAATAAATAGTAAAGTAATGTTATCTCCACTAGCAGG
AGTTACAGATAAAGTATTTAGAAAATTAGTAAGAAAATGGGCTCCGGATTCTCTTCTATTTACTGAAATGATAAATGCCA
CAAGTTTAAAGGAAGGACATGGCACTCAAAAAATTAAGCAACTGGAATTAGAACAAGGTCCTATTGGAGTACAGATCTTT
GACAATAGACCCTTTGCCGTTTCAGAGGCAGCTAAACTTGCTGAAGATTCCGGAGCATTTCTTATAGATATAAATATGGG
CTGTCCAGTAAAAAAAATCTCAAAGAAAGGGGGTGGAAGTGCTTTAATTAAAGATCGTTTATTAGCTGTTGAATTAGTAA
AGAGAGTTTCAAAAGCTGTTAAAATTCCAGTTACAGTAAAAACAAGACTTGGATGGGATAATAAAGAAGAAAATATAGAA
GAATTTCTATTAAGTCTCCAAGATTCAGGTGCAACAATGATAACCTTGCACGGGAGGACAAGAAAAGAAGGGTTTTCAGG
GGCAGCCGACTGGGATATGATTGGAAGAATTAAAGAGCATTTAGAAATACCCGTTATTGCTAATGGAGATATAAAGACTC
CTGAGGATGCATTTAATTGCTTAAAAAAAACAAATGCTGATGGTTTAATGATTGGGAGAGGCATACTTGGTTCTCCATGG
AAAATAGGAGAGATAGATTACGCAATTAAAAAAGTTAAAGGTTTTAAAGAACCTAATGTTGAAGAAAAACTACTGTTAAT
TATTGAGCATTTAGATGAATTAATTAAAGAAAAGGGAAATCATGGTTTACTAATAGCCAGAAAACATATTTCTTGGACAT
GTAAAAATTTCCAAGGAGCAAATAACCTTCGTAATGAATTAGTCAGAGCAATCAACGTTAAGGAGGTTAAAGAATTAATA
AATAAAACTATAGAGAGACTAAAAACTGATCAAACATTATTAATATAA

Upstream 100 bases:

>100_bases
TAAAAAAAAATCTGATTATTCTTTTAAGAATTAAAAAAAAAACAACAATGAATGGTAATTCATCAACTTATAATAAGTTT
ACGTAAATTATATTTTTATA

Downstream 100 bases:

>100_bases
TAATAATTTAACCTATAAAATGAAAATAATTAGTTCAAAGACAAGTGAAAGAGTTTGTAAACATATGAATAAAGATCATA
TTGAATCTGTACATAAATAC

Product: nitrogen regulation protein NifR3 family-like protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MSSVIKLRGKGLSRIINSKVMLSPLAGVTDKVFRKLVRKWAPDSLLFTEMINATSLKEGHGTQKIKQLELEQGPIGVQIF
DNRPFAVSEAAKLAEDSGAFLIDINMGCPVKKISKKGGGSALIKDRLLAVELVKRVSKAVKIPVTVKTRLGWDNKEENIE
EFLLSLQDSGATMITLHGRTRKEGFSGAADWDMIGRIKEHLEIPVIANGDIKTPEDAFNCLKKTNADGLMIGRGILGSPW
KIGEIDYAIKKVKGFKEPNVEEKLLLIIEHLDELIKEKGNHGLLIARKHISWTCKNFQGANNLRNELVRAINVKEVKELI
NKTIERLKTDQTLLI

Sequences:

>Translated_335_residues
MSSVIKLRGKGLSRIINSKVMLSPLAGVTDKVFRKLVRKWAPDSLLFTEMINATSLKEGHGTQKIKQLELEQGPIGVQIF
DNRPFAVSEAAKLAEDSGAFLIDINMGCPVKKISKKGGGSALIKDRLLAVELVKRVSKAVKIPVTVKTRLGWDNKEENIE
EFLLSLQDSGATMITLHGRTRKEGFSGAADWDMIGRIKEHLEIPVIANGDIKTPEDAFNCLKKTNADGLMIGRGILGSPW
KIGEIDYAIKKVKGFKEPNVEEKLLLIIEHLDELIKEKGNHGLLIARKHISWTCKNFQGANNLRNELVRAINVKEVKELI
NKTIERLKTDQTLLI
>Mature_334_residues
SSVIKLRGKGLSRIINSKVMLSPLAGVTDKVFRKLVRKWAPDSLLFTEMINATSLKEGHGTQKIKQLELEQGPIGVQIFD
NRPFAVSEAAKLAEDSGAFLIDINMGCPVKKISKKGGGSALIKDRLLAVELVKRVSKAVKIPVTVKTRLGWDNKEENIEE
FLLSLQDSGATMITLHGRTRKEGFSGAADWDMIGRIKEHLEIPVIANGDIKTPEDAFNCLKKTNADGLMIGRGILGSPWK
IGEIDYAIKKVKGFKEPNVEEKLLLIIEHLDELIKEKGNHGLLIARKHISWTCKNFQGANNLRNELVRAINVKEVKELIN
KTIERLKTDQTLLI

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=234, Percent_Identity=32.0512820512821, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI239788462, Length=247, Percent_Identity=31.9838056680162, Blast_Score=118, Evalue=7e-27,
Organism=Homo sapiens, GI31742496, Length=224, Percent_Identity=34.375, Blast_Score=108, Evalue=7e-24,
Organism=Homo sapiens, GI40807366, Length=228, Percent_Identity=28.9473684210526, Blast_Score=98, Evalue=9e-21,
Organism=Homo sapiens, GI8923374, Length=248, Percent_Identity=25.4032258064516, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1789660, Length=291, Percent_Identity=35.7388316151203, Blast_Score=178, Evalue=4e-46,
Organism=Escherichia coli, GI1788462, Length=308, Percent_Identity=31.8181818181818, Blast_Score=138, Evalue=4e-34,
Organism=Escherichia coli, GI145693211, Length=309, Percent_Identity=25.8899676375405, Blast_Score=100, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17507177, Length=318, Percent_Identity=28.3018867924528, Blast_Score=114, Evalue=9e-26,
Organism=Caenorhabditis elegans, GI25144369, Length=254, Percent_Identity=29.9212598425197, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17543114, Length=240, Percent_Identity=29.5833333333333, Blast_Score=99, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6323560, Length=244, Percent_Identity=31.5573770491803, Blast_Score=100, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6323433, Length=258, Percent_Identity=28.2945736434109, Blast_Score=94, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6323437, Length=242, Percent_Identity=30.1652892561983, Blast_Score=90, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24585320, Length=235, Percent_Identity=33.6170212765957, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24580595, Length=231, Percent_Identity=29.4372294372294, Blast_Score=111, Evalue=8e-25,
Organism=Drosophila melanogaster, GI19920448, Length=231, Percent_Identity=29.4372294372294, Blast_Score=111, Evalue=8e-25,
Organism=Drosophila melanogaster, GI19921524, Length=233, Percent_Identity=28.755364806867, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI45549423, Length=138, Percent_Identity=34.0579710144928, Blast_Score=69, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 37243; Mature: 37112

Theoretical pI: Translated: 10.24; Mature: 10.24

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSVIKLRGKGLSRIINSKVMLSPLAGVTDKVFRKLVRKWAPDSLLFTEMINATSLKEGH
CCCCEEECCCCHHHHHCCHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCC
GTQKIKQLELEQGPIGVQIFDNRPFAVSEAAKLAEDSGAFLIDINMGCPVKKISKKGGGS
CHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCCCCCEEEEECCCCCHHHHHCCCCCC
ALIKDRLLAVELVKRVSKAVKIPVTVKTRLGWDNKEENIEEFLLSLQDSGATMITLHGRT
HHHHHHHHHHHHHHHHHHHEECCEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEECCCC
RKEGFSGAADWDMIGRIKEHLEIPVIANGDIKTPEDAFNCLKKTNADGLMIGRGILGSPW
CCCCCCCCCCHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCC
KIGEIDYAIKKVKGFKEPNVEEKLLLIIEHLDELIKEKGNHGLLIARKHISWTCKNFQGA
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEECCCCCC
NNLRNELVRAINVKEVKELINKTIERLKTDQTLLI
HHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEECC
>Mature Secondary Structure 
SSVIKLRGKGLSRIINSKVMLSPLAGVTDKVFRKLVRKWAPDSLLFTEMINATSLKEGH
CCCEEECCCCHHHHHCCHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCC
GTQKIKQLELEQGPIGVQIFDNRPFAVSEAAKLAEDSGAFLIDINMGCPVKKISKKGGGS
CHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCCCCCEEEEECCCCCHHHHHCCCCCC
ALIKDRLLAVELVKRVSKAVKIPVTVKTRLGWDNKEENIEEFLLSLQDSGATMITLHGRT
HHHHHHHHHHHHHHHHHHHEECCEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEECCCC
RKEGFSGAADWDMIGRIKEHLEIPVIANGDIKTPEDAFNCLKKTNADGLMIGRGILGSPW
CCCCCCCCCCHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCC
KIGEIDYAIKKVKGFKEPNVEEKLLLIIEHLDELIKEKGNHGLLIARKHISWTCKNFQGA
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEECCCCCC
NNLRNELVRAINVKEVKELINKTIERLKTDQTLLI
HHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]