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The map label for this gene is btuE [H]

Identifier: 123966324

GI number: 123966324

Start: 962424

End: 962903

Strand: Direct

Name: btuE [H]

Synonym: P9515_10911

Alternate gene names: 123966324

Gene position: 962424-962903 (Clockwise)

Preceding gene: 123966320

Following gene: 123966327

Centisome position: 56.47

GC content: 32.29

Gene sequence:

>480_bases
ATGGTAATAGATGTTCAAAACACCCCTGTTCTTTCAGCGGATGGGAAATCTAAAAAGCTTGGAGAATTTTCAAAAGATGT
AATTTTAATAGTTAACGTAGCAAGTTATTGTGGCAATACAGCTCAATATGAAGATCTACAAAAGCTTCACAAAATATATT
CAAAAAAAGGTTTGAGAATACTTGCTTTCCCTTGCAATGATTTTGGTAATCAAGAGCCGGATTCCCTTTCAAAGATACAA
AATTTTTGTACTACTGAATATGGGGTAGAATTTGAAATAATGGAAAAAGTTCATGCTAAAGGTAATACTACAGAACCATA
TACAACTCTTAATAAGGTTGAACCAAAGGGAGATGTAGAATGGAATTTTGAAAAATTCTTAATTGGTAGAGATAGCAATG
TGATTGCAAGATTTAAACCTAGTGTTAAGCCTTTCGATGATAATTTGATTGCCGCTATTGAAGTCGCATTAGATTCTTAA

Upstream 100 bases:

>100_bases
CTAATAATTACAAATAAAAATAATACGGAAATATGATCTTAAAAATGACGCAAAAACTATAATTCAATGTTATCTTCTAT
ATTAATTATTTATAAAAAAT

Downstream 100 bases:

>100_bases
TACAGATAATCTTATAGAATGTAAAATCTTACTTAATTAAAAATAATTATATAAATATTTTTTGAATTTATAAAAAAATG
ATTATAGGTAGAATTATTAA

Product: glutathione peroxidase

Products: glutathione disulfide; H2O

Alternate protein names: NA

Number of amino acids: Translated: 159; Mature: 159

Protein sequence:

>159_residues
MVIDVQNTPVLSADGKSKKLGEFSKDVILIVNVASYCGNTAQYEDLQKLHKIYSKKGLRILAFPCNDFGNQEPDSLSKIQ
NFCTTEYGVEFEIMEKVHAKGNTTEPYTTLNKVEPKGDVEWNFEKFLIGRDSNVIARFKPSVKPFDDNLIAAIEVALDS

Sequences:

>Translated_159_residues
MVIDVQNTPVLSADGKSKKLGEFSKDVILIVNVASYCGNTAQYEDLQKLHKIYSKKGLRILAFPCNDFGNQEPDSLSKIQ
NFCTTEYGVEFEIMEKVHAKGNTTEPYTTLNKVEPKGDVEWNFEKFLIGRDSNVIARFKPSVKPFDDNLIAAIEVALDS
>Mature_159_residues
MVIDVQNTPVLSADGKSKKLGEFSKDVILIVNVASYCGNTAQYEDLQKLHKIYSKKGLRILAFPCNDFGNQEPDSLSKIQ
NFCTTEYGVEFEIMEKVHAKGNTTEPYTTLNKVEPKGDVEWNFEKFLIGRDSNVIARFKPSVKPFDDNLIAAIEVALDS

Specific function: Not essential for B12 transport; however, it is an auxiliary component of the transport system [H]

COG id: COG0386

COG function: function code O; Glutathione peroxidase

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutathione peroxidase family [H]

Homologues:

Organism=Homo sapiens, GI75709200, Length=139, Percent_Identity=38.8489208633094, Blast_Score=100, Evalue=8e-22,
Organism=Homo sapiens, GI90903240, Length=139, Percent_Identity=38.8489208633094, Blast_Score=99, Evalue=2e-21,
Organism=Homo sapiens, GI15618997, Length=130, Percent_Identity=38.4615384615385, Blast_Score=92, Evalue=3e-19,
Organism=Homo sapiens, GI90903238, Length=120, Percent_Identity=40, Blast_Score=87, Evalue=6e-18,
Organism=Homo sapiens, GI192455698, Length=132, Percent_Identity=35.6060606060606, Blast_Score=87, Evalue=8e-18,
Organism=Homo sapiens, GI6006001, Length=143, Percent_Identity=34.965034965035, Blast_Score=72, Evalue=3e-13,
Organism=Homo sapiens, GI41406084, Length=163, Percent_Identity=30.6748466257669, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI33186887, Length=135, Percent_Identity=33.3333333333333, Blast_Score=68, Evalue=3e-12,
Organism=Escherichia coli, GI1788003, Length=177, Percent_Identity=38.9830508474576, Blast_Score=125, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17535473, Length=160, Percent_Identity=38.75, Blast_Score=110, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17506887, Length=142, Percent_Identity=39.4366197183099, Blast_Score=104, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71993584, Length=138, Percent_Identity=37.6811594202899, Blast_Score=103, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI193210707, Length=146, Percent_Identity=39.041095890411, Blast_Score=100, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17550320, Length=175, Percent_Identity=31.4285714285714, Blast_Score=81, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI193209493, Length=175, Percent_Identity=29.7142857142857, Blast_Score=76, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI71993573, Length=86, Percent_Identity=40.6976744186046, Blast_Score=70, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6319721, Length=135, Percent_Identity=43.7037037037037, Blast_Score=116, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6322228, Length=123, Percent_Identity=43.9024390243902, Blast_Score=111, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6322826, Length=154, Percent_Identity=36.3636363636364, Blast_Score=105, Evalue=2e-24,
Organism=Drosophila melanogaster, GI85726443, Length=133, Percent_Identity=38.3458646616541, Blast_Score=87, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24656772, Length=138, Percent_Identity=33.3333333333333, Blast_Score=80, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24656767, Length=138, Percent_Identity=33.3333333333333, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24656777, Length=138, Percent_Identity=33.3333333333333, Blast_Score=79, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000889
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00255 GSHPx [H]

EC number: 1.11.1.9

Molecular weight: Translated: 17825; Mature: 17825

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVIDVQNTPVLSADGKSKKLGEFSKDVILIVNVASYCGNTAQYEDLQKLHKIYSKKGLRI
CEEECCCCCEEECCCCCCCCCCCCCCEEEEEEEHHHCCCCCCHHHHHHHHHHHHCCCCEE
LAFPCNDFGNQEPDSLSKIQNFCTTEYGVEFEIMEKVHAKGNTTEPYTTLNKVEPKGDVE
EEECCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCHHCCCCCCCCCC
WNFEKFLIGRDSNVIARFKPSVKPFDDNLIAAIEVALDS
CCCEEEEEECCCCEEEEECCCCCCCCCCEEEEEEEEECC
>Mature Secondary Structure
MVIDVQNTPVLSADGKSKKLGEFSKDVILIVNVASYCGNTAQYEDLQKLHKIYSKKGLRI
CEEECCCCCEEECCCCCCCCCCCCCCEEEEEEEHHHCCCCCCHHHHHHHHHHHHCCCCEE
LAFPCNDFGNQEPDSLSKIQNFCTTEYGVEFEIMEKVHAKGNTTEPYTTLNKVEPKGDVE
EEECCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCHHCCCCCCCCCC
WNFEKFLIGRDSNVIARFKPSVKPFDDNLIAAIEVALDS
CCCEEEEEECCCCEEEEECCCCCCCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glutathione; H2O2

Specific reaction: 2 glutathione + H2O2 = glutathione disulfide + 2 H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3528129; 9097039; 9278503 [H]