Click here to switch to the map view.

The map label for this gene is purT

Identifier: 123966322

GI number: 123966322

Start: 960843

End: 962015

Strand: Reverse

Name: purT

Synonym: P9515_10891

Alternate gene names: 123966322

Gene position: 962015-960843 (Counterclockwise)

Preceding gene: 123966323

Following gene: 123966321

Centisome position: 56.45

GC content: 31.71

Gene sequence:

>1173_bases
ATGGACAGTTTTAAATTTATTCAAAAGAAAATATTATTACTTGGTAGCGGCGAACTTGGAAAAGAGTTAGTGGTTGAAGC
TAAAAGATTAGGTTTAGAAGTAATAGCTATTGATAAGTACGAAAATGCACCCGCAATGCAATTAGCTGATCATTCTTTTG
TAATAAATATGAATGATAAAAAAACTTTAATAAATACGATAAAAAAAATAGAGCCTGATTTTGTAGTTCCTGAAATTGAA
GCACTCTCGATAGAAGCTTTAAAGGAATTGGAAGAAGAAGGAATAAATATTGTCCCAAATGCTAGAACTGTTGAAATTAC
AATGAATAGGCATAAAATAAGAGATCTTGCATCTAAGGAATTAAACATAAAAACTGCAAATTTTAGTTACGTATTTAATG
CTGAAGAGCTAGAGATGAAATCGTCAGAAATTGGATTCCCTCTTTTGTTAAAGCCTCTAATGAGTTCATCCGGTAAAGGA
CAAAGTTTAGTTAAAAGGAAAGAGGATCTTTTGTTAGCTTGGGAAGATGCTCTGAAAAACTCAAGAGGTAAGATAGAAGG
TGTAATTCTCGAGGAATTTTTAAATTTTGATTATGAATTTACTCTTTTAACAGTAAGAAAAAGTAGTGGAGAAAATGTAT
TTTGTGAACCAATAGGTCATGAGCAATACAAAGGAGATTATCAATGTAGTTGGCAACCATTGGACATGAATGAATCTTTA
ATTAATGAAGCTAGAAATATGACAAATAAAATATTAAATAATCTAAATGGTTCGGGTATATATGGAGTTGAGTTTTTTGT
TAGAGGCAAAGAGGTTATATTCTCAGAATTGTCTCCTAGACCTCATGACACGGGAATGGTCACCTTGGTAAGTCAAAACT
TAAACGAATTTGAATTACACTTAAGAGCCTTTTTAAATCTACCAATACCAAAAATTTCCTTATTAAAGCCATCAGCAACA
AGAGTAATACTTTCAGAAAAAGAATCTATTAACCCCTCATACACTGGACTTAATGAGGCACTTGAAGTAGAGAATACTAA
AGTTCTAATTTTTGGGAAACCAACCTCAAAGAAAGGAAGGAGAATGGGGGTAGTCCTTTCGTCTGATGATGATTTAAATA
TTGCAAGAAAAAATGCTGACAAGTCAGCATCTAAAATTAAAATAGTATCCTAA

Upstream 100 bases:

>100_bases
TTGATAACAATGCATTTTTGTTTAGTTCAAAATCCGCTAAAAGAAAAGATAAGCTTTCAAACAAGTCATCCGATAATGAT
TAAACATAAATTATTCAGTA

Downstream 100 bases:

>100_bases
TTAAAATAGTATTAAATAAATATTATAAAAAAACTACTTATTTCCTTTGTTTTTGTTTTTAATTTCTGTATGTAATATTT
AGATATATTATTTTTCTTTT

Product: phosphoribosylglycinamide formyltransferase 2

Products: 5'-Phosphoribosyl-N-Formylglycinamide; Pyrophosphate; acetylphosphate; ADP [C]

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MDSFKFIQKKILLLGSGELGKELVVEAKRLGLEVIAIDKYENAPAMQLADHSFVINMNDKKTLINTIKKIEPDFVVPEIE
ALSIEALKELEEEGINIVPNARTVEITMNRHKIRDLASKELNIKTANFSYVFNAEELEMKSSEIGFPLLLKPLMSSSGKG
QSLVKRKEDLLLAWEDALKNSRGKIEGVILEEFLNFDYEFTLLTVRKSSGENVFCEPIGHEQYKGDYQCSWQPLDMNESL
INEARNMTNKILNNLNGSGIYGVEFFVRGKEVIFSELSPRPHDTGMVTLVSQNLNEFELHLRAFLNLPIPKISLLKPSAT
RVILSEKESINPSYTGLNEALEVENTKVLIFGKPTSKKGRRMGVVLSSDDDLNIARKNADKSASKIKIVS

Sequences:

>Translated_390_residues
MDSFKFIQKKILLLGSGELGKELVVEAKRLGLEVIAIDKYENAPAMQLADHSFVINMNDKKTLINTIKKIEPDFVVPEIE
ALSIEALKELEEEGINIVPNARTVEITMNRHKIRDLASKELNIKTANFSYVFNAEELEMKSSEIGFPLLLKPLMSSSGKG
QSLVKRKEDLLLAWEDALKNSRGKIEGVILEEFLNFDYEFTLLTVRKSSGENVFCEPIGHEQYKGDYQCSWQPLDMNESL
INEARNMTNKILNNLNGSGIYGVEFFVRGKEVIFSELSPRPHDTGMVTLVSQNLNEFELHLRAFLNLPIPKISLLKPSAT
RVILSEKESINPSYTGLNEALEVENTKVLIFGKPTSKKGRRMGVVLSSDDDLNIARKNADKSASKIKIVS
>Mature_390_residues
MDSFKFIQKKILLLGSGELGKELVVEAKRLGLEVIAIDKYENAPAMQLADHSFVINMNDKKTLINTIKKIEPDFVVPEIE
ALSIEALKELEEEGINIVPNARTVEITMNRHKIRDLASKELNIKTANFSYVFNAEELEMKSSEIGFPLLLKPLMSSSGKG
QSLVKRKEDLLLAWEDALKNSRGKIEGVILEEFLNFDYEFTLLTVRKSSGENVFCEPIGHEQYKGDYQCSWQPLDMNESL
INEARNMTNKILNNLNGSGIYGVEFFVRGKEVIFSELSPRPHDTGMVTLVSQNLNEFELHLRAFLNLPIPKISLLKPSAT
RVILSEKESINPSYTGLNEALEVENTKVLIFGKPTSKKGRRMGVVLSSDDDLNIARKNADKSASKIKIVS

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1788155, Length=379, Percent_Identity=44.8548812664908, Blast_Score=356, Evalue=1e-99,
Organism=Escherichia coli, GI1786733, Length=314, Percent_Identity=24.203821656051, Blast_Score=69, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6324702, Length=383, Percent_Identity=27.154046997389, Blast_Score=115, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PURT_PROM5 (A2BWY5)

Other databases:

- EMBL:   CP000552
- RefSeq:   YP_001011403.1
- ProteinModelPortal:   A2BWY5
- SMR:   A2BWY5
- STRING:   A2BWY5
- GeneID:   4719070
- GenomeReviews:   CP000552_GR
- KEGG:   pmc:P9515_10891
- eggNOG:   COG0027
- HOGENOM:   HBG516369
- OMA:   TMNRKAI
- ProtClustDB:   PRK09288
- BioCyc:   PMAR167542:P9515ORF_1136-MONOMER
- HAMAP:   MF_01643
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862
- InterPro:   IPR011054
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- PANTHER:   PTHR23047:SF2
- TIGRFAMs:   TIGR01142

Pfam domain/function: PF02222 ATP-grasp; SSF52440 PreATP-grasp-like; SSF51246 Rudmnt_hyb_motif

EC number: 2.1.2.- [C]

Molecular weight: Translated: 43835; Mature: 43835

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS50975 ATP_GRASP

Important sites: BINDING 78-78 BINDING 110-110 BINDING 151-151 BINDING 199-199 BINDING 264-264 BINDING 276-276 BINDING 283-283 BINDING 353-353

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSFKFIQKKILLLGSGELGKELVVEAKRLGLEVIAIDKYENAPAMQLADHSFVINMNDK
CCHHHHHHHEEEEEECCCCCHHHHHHHHHCCEEEEEEECCCCCCCEEECCCEEEEEECCH
KTLINTIKKIEPDFVVPEIEALSIEALKELEEEGINIVPNARTVEITMNRHKIRDLASKE
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCEECCCCEEEEEEECHHHHHHHHHCC
LNIKTANFSYVFNAEELEMKSSEIGFPLLLKPLMSSSGKGQSLVKRKEDLLLAWEDALKN
CEEEEECEEEEECHHHHHCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHEEEEEHHHHCC
SRGKIEGVILEEFLNFDYEFTLLTVRKSSGENVFCEPIGHEQYKGDYQCSWQPLDMNESL
CCCCEEEEEEHHHHCCCEEEEEEEEEECCCCEEEEECCCCCCCCCCCEEEEEECCCCHHH
INEARNMTNKILNNLNGSGIYGVEFFVRGKEVIFSELSPRPHDTGMVTLVSQNLNEFELH
HHHHHHHHHHHHHCCCCCCEEEEEEEEECHHHHHHHCCCCCCCCCEEEEEECCCCHHHHH
LRAFLNLPIPKISLLKPSATRVILSEKESINPSYTGLNEALEVENTKVLIFGKPTSKKGR
HHHHHCCCCCCEEEECCCCEEEEEECCCCCCCCCCCCCHHEEECCEEEEEEECCCCCCCC
RMGVVLSSDDDLNIARKNADKSASKIKIVS
EEEEEEECCCCCEEECCCCCCCCCEEEEEC
>Mature Secondary Structure
MDSFKFIQKKILLLGSGELGKELVVEAKRLGLEVIAIDKYENAPAMQLADHSFVINMNDK
CCHHHHHHHEEEEEECCCCCHHHHHHHHHCCEEEEEEECCCCCCCEEECCCEEEEEECCH
KTLINTIKKIEPDFVVPEIEALSIEALKELEEEGINIVPNARTVEITMNRHKIRDLASKE
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCEECCCCEEEEEEECHHHHHHHHHCC
LNIKTANFSYVFNAEELEMKSSEIGFPLLLKPLMSSSGKGQSLVKRKEDLLLAWEDALKN
CEEEEECEEEEECHHHHHCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHEEEEEHHHHCC
SRGKIEGVILEEFLNFDYEFTLLTVRKSSGENVFCEPIGHEQYKGDYQCSWQPLDMNESL
CCCCEEEEEEHHHHCCCEEEEEEEEEECCCCEEEEECCCCCCCCCCCEEEEEECCCCHHH
INEARNMTNKILNNLNGSGIYGVEFFVRGKEVIFSELSPRPHDTGMVTLVSQNLNEFELH
HHHHHHHHHHHHHCCCCCCEEEEEEEEECHHHHHHHCCCCCCCCCEEEEEECCCCHHHHH
LRAFLNLPIPKISLLKPSATRVILSEKESINPSYTGLNEALEVENTKVLIFGKPTSKKGR
HHHHHCCCCCCEEEECCCCEEEEEECCCCCCCCCCCCCHHEEECCEEEEEEECCCCCCCC
RMGVVLSSDDDLNIARKNADKSASKIKIVS
EEEEEEECCCCCEEECCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Formate; 5'-Phospho-Ribosylglycinamide; acetate; ATP [C]

Specific reaction: Formate + ATP + 5'-Phospho-Ribosylglycinamide = 5'-Phosphoribosyl-N-Formylglycinamide + ADP + Pyrophosphate. acetate + ATP = acetylphosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA