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The map label for this gene is ilvE [H]

Identifier: 123966193

GI number: 123966193

Start: 852504

End: 853418

Strand: Reverse

Name: ilvE [H]

Synonym: P9515_09601

Alternate gene names: 123966193

Gene position: 853418-852504 (Counterclockwise)

Preceding gene: 123966195

Following gene: 123966192

Centisome position: 50.08

GC content: 32.9

Gene sequence:

>915_bases
ATGCAAGAATTTCTTCCATATGCCTGGTTTGAAGGTAAGTGTATACCTTTTAAAGACGCAAAAGTATCTATAGCAACTCA
TTCACTTCATTATGGAACAGCCGCCTTTGGAGGAATGCGGGCTATCCCAAATCCCGAAGATAATAATGAATTTCTCTTAT
TTAGAACAGATAAACATATAAAAAGGTTATCTCAAAGCGCCAAATTGCTTTTAGCAGAAATATCTGAAGATTATATTTTT
AATGCCATTGAAACAATTTTAAAAATTAATAAACCATCAAAACCAATCTACATAAGACCATTTGTCTATACTAGTGATTT
AGGTATAGCTCCAAGATTGCATAATATAGAAACCGATTTCTTTATTTATTGCATTGAACTGGGAGATTACTTATCTCCGG
ATGGTGTATCTTGCAGAATGAGTAGTTGGACAAGACAAGAAGATAGATCTTTGCCATTAAGAGGAAAAATAAGTGGTGCT
TACATTACAAGTTCATTAGCTAAAACAGAAGCTAGTTTATCTGGGTTTGATGAAGCTTTACTTTTAAATTCAAGTGGAAA
AGTAAGTGAAGCAAGTGGTATGAATTTATTTATAGTCAGAAATGGTGAATTGATAACACCAGGTGTTGATCAAGACATTC
TGGAAGGAATCACGAGAGCTAGTGTTCTTGAATTAGCTAAGTCTTTTGGAATAAATGTTATAGAAAGACCCGTTGATAAG
ACTGAGTTACTTATTGCAGATGAGGTGTTTCTAACTGGTACTGCGGCTAAAATTACACCGGTAAAACAAATAGAATCTAC
TAGAATTGATAAAAAACGTCCAATAATGAATAAACTGAAAAATAAGCTTATAGAAATAACGGAAGGGCGATCACAAGACT
ATGATAGTTGGATTACTCGTATTAAAATTAATTAA

Upstream 100 bases:

>100_bases
TTTCCCGCAATATTTAATATCCTATGCATTTAAATATAATAATTAGGTTTAGAATACTTAAGTAAGTTAGAACAATTTTA
ACTCATTTCTAAAATTAAAT

Downstream 100 bases:

>100_bases
TTTAGGTTTTCGTATAAACATATGGTTTCATTTAGGGATTATTTAAATAGAGATGATAAACCAATTATAATTTTCGATGG
TGGTACAGGCACTTCTTTTC

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT [H]

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MQEFLPYAWFEGKCIPFKDAKVSIATHSLHYGTAAFGGMRAIPNPEDNNEFLLFRTDKHIKRLSQSAKLLLAEISEDYIF
NAIETILKINKPSKPIYIRPFVYTSDLGIAPRLHNIETDFFIYCIELGDYLSPDGVSCRMSSWTRQEDRSLPLRGKISGA
YITSSLAKTEASLSGFDEALLLNSSGKVSEASGMNLFIVRNGELITPGVDQDILEGITRASVLELAKSFGINVIERPVDK
TELLIADEVFLTGTAAKITPVKQIESTRIDKKRPIMNKLKNKLIEITEGRSQDYDSWITRIKIN

Sequences:

>Translated_304_residues
MQEFLPYAWFEGKCIPFKDAKVSIATHSLHYGTAAFGGMRAIPNPEDNNEFLLFRTDKHIKRLSQSAKLLLAEISEDYIF
NAIETILKINKPSKPIYIRPFVYTSDLGIAPRLHNIETDFFIYCIELGDYLSPDGVSCRMSSWTRQEDRSLPLRGKISGA
YITSSLAKTEASLSGFDEALLLNSSGKVSEASGMNLFIVRNGELITPGVDQDILEGITRASVLELAKSFGINVIERPVDK
TELLIADEVFLTGTAAKITPVKQIESTRIDKKRPIMNKLKNKLIEITEGRSQDYDSWITRIKIN
>Mature_304_residues
MQEFLPYAWFEGKCIPFKDAKVSIATHSLHYGTAAFGGMRAIPNPEDNNEFLLFRTDKHIKRLSQSAKLLLAEISEDYIF
NAIETILKINKPSKPIYIRPFVYTSDLGIAPRLHNIETDFFIYCIELGDYLSPDGVSCRMSSWTRQEDRSLPLRGKISGA
YITSSLAKTEASLSGFDEALLLNSSGKVSEASGMNLFIVRNGELITPGVDQDILEGITRASVLELAKSFGINVIERPVDK
TELLIADEVFLTGTAAKITPVKQIESTRIDKKRPIMNKLKNKLIEITEGRSQDYDSWITRIKIN

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=304, Percent_Identity=32.8947368421053, Blast_Score=189, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6322608, Length=289, Percent_Identity=26.2975778546713, Blast_Score=80, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6322002, Length=329, Percent_Identity=24.0121580547112, Blast_Score=76, Evalue=8e-15,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005785 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 34100; Mature: 34100

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS00037 MYB_1 ; PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQEFLPYAWFEGKCIPFKDAKVSIATHSLHYGTAAFGGMRAIPNPEDNNEFLLFRTDKHI
CCCCCCCCEECCEEECCCCCEEEEEEEEEECCHHHHCCCCCCCCCCCCCEEEEEECCHHH
KRLSQSAKLLLAEISEDYIFNAIETILKINKPSKPIYIRPFVYTSDLGIAPRLHNIETDF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCE
FIYCIELGDYLSPDGVSCRMSSWTRQEDRSLPLRGKISGAYITSSLAKTEASLSGFDEAL
EEEEEECCCCCCCCCCEEECCCCCCHHHCCCCCEECCCCHHHHHHHHHHHHHHCCCCCEE
LLNSSGKVSEASGMNLFIVRNGELITPGVDQDILEGITRASVLELAKSFGINVIERPVDK
EECCCCCCCCCCCCEEEEEECCEEECCCCCHHHHHHHHHHHHHHHHHHHCCEEECCCCCC
TELLIADEVFLTGTAAKITPVKQIESTRIDKKRPIMNKLKNKLIEITEGRSQDYDSWITR
CEEEEEEEEEEECCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHEEECCCCCCCCCCEEEE
IKIN
EEEC
>Mature Secondary Structure
MQEFLPYAWFEGKCIPFKDAKVSIATHSLHYGTAAFGGMRAIPNPEDNNEFLLFRTDKHI
CCCCCCCCEECCEEECCCCCEEEEEEEEEECCHHHHCCCCCCCCCCCCCEEEEEECCHHH
KRLSQSAKLLLAEISEDYIFNAIETILKINKPSKPIYIRPFVYTSDLGIAPRLHNIETDF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCE
FIYCIELGDYLSPDGVSCRMSSWTRQEDRSLPLRGKISGAYITSSLAKTEASLSGFDEAL
EEEEEECCCCCCCCCCEEECCCCCCHHHCCCCCEECCCCHHHHHHHHHHHHHHCCCCCEE
LLNSSGKVSEASGMNLFIVRNGELITPGVDQDILEGITRASVLELAKSFGINVIERPVDK
EECCCCCCCCCCCCEEEEEECCEEECCCCCHHHHHHHHHHHHHHHHHHHCCEEECCCCCC
TELLIADEVFLTGTAAKITPVKQIESTRIDKKRPIMNKLKNKLIEITEGRSQDYDSWITR
CEEEEEEEEEEECCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHEEECCCCCCCCCCEEEE
IKIN
EEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]