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The map label for this gene is tsf [H]

Identifier: 123966063

GI number: 123966063

Start: 752639

End: 753295

Strand: Reverse

Name: tsf [H]

Synonym: P9515_08301

Alternate gene names: 123966063

Gene position: 753295-752639 (Counterclockwise)

Preceding gene: 123966064

Following gene: 123966062

Centisome position: 44.2

GC content: 35.77

Gene sequence:

>657_bases
ATGGCAAACATTACAGCAAAACTTGTAAAAGATCTTAGAGATAAGACTGGAGCCGGGATGATGGATTGTAAAAAGGCTTT
AAATGAGACAGATGGAAATGTTGAAAAAGCCCTAGAGTGGTTAAGAAAAAAAGGAATCGCAAGTGCAGAGAAGAAATCGG
GGAGAGTTGCTGCGGAAGGTTCTATAGGCAGTTATATTCATACAGGTTCTAGAGTAGGTGTTCTTTTAGAACTAAATTGT
GAAACTGATTTCGTTGCTAGAGGAGATATTTTTCAATCTCTTTTAAAGGATGTATCGATGCAAGTAGCCGCATGTCCAAA
TGTTGAATACGTTTCTATTGATGAAATACCGGAAGATGTTGTAAAAAAAGAAAAAGGAATAGAAATGGGTAGAGATGACT
TGTCTGGTAAGCCCGAAAATATTAAAGAAAAAATTGTTGAAGGTAGAATTGCTAAAAGACTAAATGAGTTGGTCTTGCTT
TCTCAACCTTATATAAAGGATAGTTCTCTAACAGTAGAAGATTTAGTAAAGCAGGCAGCAGCTAAAATTGGTGAAAATAT
CAAAGTGAGGCGTTTCACAAGATATACGTTAGGTGAGGGTATTGAAAAGAATGAAATGGACTTTGCCGATGAAGTCGCAT
CTTTAAAGTCAAATTAA

Upstream 100 bases:

>100_bases
GACTTGCAGATGCAATTAATGAAGGTAGAAAAGGTTCTAATGATCAAAGAAAAGTTTAATTTTTATTAAAACTTCAAAAA
ACCAAAAAATTATTATTTAA

Downstream 100 bases:

>100_bases
TAAATTTGGATAATAGTATCAAAGGTAGATATTCAAAAGAGGTGAAGATTCAGCTCGATAGGGCTGAGGTTAAAAAAGAT
ATATTATTAAAAAATATTTA

Product: elongation factor Ts

Products: NA

Alternate protein names: EF-Ts [H]

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MANITAKLVKDLRDKTGAGMMDCKKALNETDGNVEKALEWLRKKGIASAEKKSGRVAAEGSIGSYIHTGSRVGVLLELNC
ETDFVARGDIFQSLLKDVSMQVAACPNVEYVSIDEIPEDVVKKEKGIEMGRDDLSGKPENIKEKIVEGRIAKRLNELVLL
SQPYIKDSSLTVEDLVKQAAAKIGENIKVRRFTRYTLGEGIEKNEMDFADEVASLKSN

Sequences:

>Translated_218_residues
MANITAKLVKDLRDKTGAGMMDCKKALNETDGNVEKALEWLRKKGIASAEKKSGRVAAEGSIGSYIHTGSRVGVLLELNC
ETDFVARGDIFQSLLKDVSMQVAACPNVEYVSIDEIPEDVVKKEKGIEMGRDDLSGKPENIKEKIVEGRIAKRLNELVLL
SQPYIKDSSLTVEDLVKQAAAKIGENIKVRRFTRYTLGEGIEKNEMDFADEVASLKSN
>Mature_217_residues
ANITAKLVKDLRDKTGAGMMDCKKALNETDGNVEKALEWLRKKGIASAEKKSGRVAAEGSIGSYIHTGSRVGVLLELNCE
TDFVARGDIFQSLLKDVSMQVAACPNVEYVSIDEIPEDVVKKEKGIEMGRDDLSGKPENIKEKIVEGRIAKRLNELVLLS
QPYIKDSSLTVEDLVKQAAAKIGENIKVRRFTRYTLGEGIEKNEMDFADEVASLKSN

Specific function: Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome [H]

COG id: COG0264

COG function: function code J; Translation elongation factor Ts

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EF-Ts family [H]

Homologues:

Organism=Homo sapiens, GI291084500, Length=189, Percent_Identity=31.7460317460317, Blast_Score=68, Evalue=5e-12,
Organism=Homo sapiens, GI291084498, Length=97, Percent_Identity=42.2680412371134, Blast_Score=68, Evalue=6e-12,
Organism=Homo sapiens, GI291084502, Length=97, Percent_Identity=42.2680412371134, Blast_Score=68, Evalue=6e-12,
Organism=Homo sapiens, GI171846268, Length=97, Percent_Identity=42.2680412371134, Blast_Score=67, Evalue=9e-12,
Organism=Escherichia coli, GI1786366, Length=94, Percent_Identity=55.3191489361702, Blast_Score=104, Evalue=5e-24,
Organism=Drosophila melanogaster, GI19921466, Length=115, Percent_Identity=39.1304347826087, Blast_Score=72, Evalue=2e-13,

Paralogues:

None

Copy number: 2670 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1100 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 4173 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 14563 Molecules/Cell In: Growth Phase, Gluco

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001816
- InterPro:   IPR014039
- InterPro:   IPR018101
- InterPro:   IPR009060
- InterPro:   IPR000449 [H]

Pfam domain/function: PF00889 EF_TS; PF00627 UBA [H]

EC number: NA

Molecular weight: Translated: 23992; Mature: 23861

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: PS01126 EF_TS_1 ; PS01127 EF_TS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANITAKLVKDLRDKTGAGMMDCKKALNETDGNVEKALEWLRKKGIASAEKKSGRVAAEG
CCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHCCCEEECC
SIGSYIHTGSRVGVLLELNCETDFVARGDIFQSLLKDVSMQVAACPNVEYVSIDEIPEDV
CCCHHEECCCCEEEEEEECCCCCCEECCHHHHHHHHHHHHHHHCCCCCCEEEHHHCHHHH
VKKEKGIEMGRDDLSGKPENIKEKIVEGRIAKRLNELVLLSQPYIKDSSLTVEDLVKQAA
HHHHHCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
AKIGENIKVRRFTRYTLGEGIEKNEMDFADEVASLKSN
HHHCCCCEEHHHHHHHHHCCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
ANITAKLVKDLRDKTGAGMMDCKKALNETDGNVEKALEWLRKKGIASAEKKSGRVAAEG
CCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHCCCEEECC
SIGSYIHTGSRVGVLLELNCETDFVARGDIFQSLLKDVSMQVAACPNVEYVSIDEIPEDV
CCCHHEECCCCEEEEEEECCCCCCEECCHHHHHHHHHHHHHHHCCCCCCEEEHHHCHHHH
VKKEKGIEMGRDDLSGKPENIKEKIVEGRIAKRLNELVLLSQPYIKDSSLTVEDLVKQAA
HHHHHCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
AKIGENIKVRRFTRYTLGEGIEKNEMDFADEVASLKSN
HHHCCCCEEHHHHHHHHHCCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA