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The map label for this gene is hemA

Identifier: 123966050

GI number: 123966050

Start: 734720

End: 736027

Strand: Reverse

Name: hemA

Synonym: P9515_08151

Alternate gene names: 123966050

Gene position: 736027-734720 (Counterclockwise)

Preceding gene: 123966051

Following gene: 123966049

Centisome position: 43.19

GC content: 33.26

Gene sequence:

>1308_bases
ATGCAAATTGTTGTCGTCGGACTAAGTCATCGCACGGCACCTGTTGAAGTGCGAGAAAAGTTAAGTATTCCGGATCAAGC
TATTTCTGAATCACTAAAAAGTCTTAGGGCCTATTCTGACATTTTAGAAGTTTCCATTTTAAGTACTTGCAACAGGTTAG
AGATATACGGTCTTGTAAAAGATAAAAATATAGGTATTTCATCAATTAAGGAATTTTTATCAGATTATTCTCAAGTTAAT
TGTGAAGTTCTAACTCCTCATTTATTTGACTTTAGGCAAGAAGAGGCTGTTTTGCATTTAATGAAAGTATCAGCTGGATT
AGATAGCCTTGTTCTGGGAGAAGGGCAAATTCTCTCACAAGTAAAAAAAATGATGAGATTAGGTCAGGAGAATCAATCTA
CTGGCCCAATTCTTAATAGATTATTAAGTCAATCAGTTAGCGCAGGAAAAAAAGTAAGAGCTGAAACGAACTTAGGAACT
GGTGCTGTATCGATAAGTTCAGCGGCTGTGGAACTAGCCCAATTAAAAATTGGTCAAGATAACGGCATTGATGGGCTTGT
TAGTTTGAAAGGAGAAAAAGTTCTTGTCGTTGGAGCTGGAAGGATGAGTAGACTGTTAATTACGCATTTGAAATCTAAAG
GATGTAATAAGCTCACCCTTTTAAATAGAAATATTGATAGAGCTATCAATCTTTCAGGTGATTTCCCAGATATTGAAATT
ATTTGTAAGGGTTTAGATGAATTGGATAAAAGCATAACATTATCTTCACTAGTCTTCACAAGTACCGCTTCTGAAAAACC
ATTTATTGATTTAGAAAGAATTAAAAATATAAGTTTAAATAATAAACTTAAATTTATTGATATAGGTGTACCAAGAAATA
TTTCTAATGATGTAAAACAACACGATTTAATAGAAGCATTTGATGTCGATGACTTAGAAGAAGTTGTTTCTAGAAATCAA
GAATTCAGGCAAAAAATAGCAAAAGAGGCTGAGTCTTTAGTGAAAGAGGAAAGAATCATTTTTTTGGAGTGGTGGGCAAG
TTTAGAAGCAGTTCCAGTAATAAATAAATTAAGGTCTGATTTGGAACTTATAAGAAAAGAAGAGTTACAAAAAGCACTAA
GTAGAATGGGGCCGGATTTTTCTGCTCGAGAAAGAAAAGTTGTGGAGGCTTTAACTAAAGGAATAATTAATAAAATTCTT
CACACACCAGTTACAAAATTGAGAAGTCCTCAGTCGAGAGAAGAAAGACAAGCATCATTAAAAATCGTAGAAAAATTGTT
TTCTTTAATGGATGATGAGCAAAAATAA

Upstream 100 bases:

>100_bases
TAGTAATAAGTACTTTAGATAGTACATGTCGATTTACAAATACTATTCATATGAAAGATGGCGCTAAAAGCATCAATCTT
TAATCTTTTTATTTAATTTT

Downstream 100 bases:

>100_bases
TTAAAAATTTCTGACTTTATATTAAGTTTTTCATTAGATTTATCTAAATACCTTTGTAAAGTGGCATTTTTCATTTTTAT
ATTTGCACATAATCAGTTAA

Product: glutamyl-tRNA reductase

Products: NA

Alternate protein names: GluTR

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MQIVVVGLSHRTAPVEVREKLSIPDQAISESLKSLRAYSDILEVSILSTCNRLEIYGLVKDKNIGISSIKEFLSDYSQVN
CEVLTPHLFDFRQEEAVLHLMKVSAGLDSLVLGEGQILSQVKKMMRLGQENQSTGPILNRLLSQSVSAGKKVRAETNLGT
GAVSISSAAVELAQLKIGQDNGIDGLVSLKGEKVLVVGAGRMSRLLITHLKSKGCNKLTLLNRNIDRAINLSGDFPDIEI
ICKGLDELDKSITLSSLVFTSTASEKPFIDLERIKNISLNNKLKFIDIGVPRNISNDVKQHDLIEAFDVDDLEEVVSRNQ
EFRQKIAKEAESLVKEERIIFLEWWASLEAVPVINKLRSDLELIRKEELQKALSRMGPDFSARERKVVEALTKGIINKIL
HTPVTKLRSPQSREERQASLKIVEKLFSLMDDEQK

Sequences:

>Translated_435_residues
MQIVVVGLSHRTAPVEVREKLSIPDQAISESLKSLRAYSDILEVSILSTCNRLEIYGLVKDKNIGISSIKEFLSDYSQVN
CEVLTPHLFDFRQEEAVLHLMKVSAGLDSLVLGEGQILSQVKKMMRLGQENQSTGPILNRLLSQSVSAGKKVRAETNLGT
GAVSISSAAVELAQLKIGQDNGIDGLVSLKGEKVLVVGAGRMSRLLITHLKSKGCNKLTLLNRNIDRAINLSGDFPDIEI
ICKGLDELDKSITLSSLVFTSTASEKPFIDLERIKNISLNNKLKFIDIGVPRNISNDVKQHDLIEAFDVDDLEEVVSRNQ
EFRQKIAKEAESLVKEERIIFLEWWASLEAVPVINKLRSDLELIRKEELQKALSRMGPDFSARERKVVEALTKGIINKIL
HTPVTKLRSPQSREERQASLKIVEKLFSLMDDEQK
>Mature_435_residues
MQIVVVGLSHRTAPVEVREKLSIPDQAISESLKSLRAYSDILEVSILSTCNRLEIYGLVKDKNIGISSIKEFLSDYSQVN
CEVLTPHLFDFRQEEAVLHLMKVSAGLDSLVLGEGQILSQVKKMMRLGQENQSTGPILNRLLSQSVSAGKKVRAETNLGT
GAVSISSAAVELAQLKIGQDNGIDGLVSLKGEKVLVVGAGRMSRLLITHLKSKGCNKLTLLNRNIDRAINLSGDFPDIEI
ICKGLDELDKSITLSSLVFTSTASEKPFIDLERIKNISLNNKLKFIDIGVPRNISNDVKQHDLIEAFDVDDLEEVVSRNQ
EFRQKIAKEAESLVKEERIIFLEWWASLEAVPVINKLRSDLELIRKEELQKALSRMGPDFSARERKVVEALTKGIINKIL
HTPVTKLRSPQSREERQASLKIVEKLFSLMDDEQK

Specific function: Catalyzes the NADPH-dependent reduction of glutamyl- tRNA(Glu) to glutamate 1-semialdehyde (GSA)

COG id: COG0373

COG function: function code H; Glutamyl-tRNA reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamyl-tRNA reductase family

Homologues:

Organism=Escherichia coli, GI1787461, Length=413, Percent_Identity=33.8983050847458, Blast_Score=198, Evalue=7e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM1_PROM5 (A2BW63)

Other databases:

- EMBL:   CP000552
- RefSeq:   YP_001011131.1
- ProteinModelPortal:   A2BW63
- STRING:   A2BW63
- GeneID:   4719397
- GenomeReviews:   CP000552_GR
- KEGG:   pmc:P9515_08151
- eggNOG:   COG0373
- HOGENOM:   HBG732626
- OMA:   GPILNRL
- ProtClustDB:   PRK00045
- BioCyc:   PMAR167542:P9515ORF_0856-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00087
- InterPro:   IPR000343
- InterPro:   IPR015896
- InterPro:   IPR015895
- InterPro:   IPR016040
- InterPro:   IPR018214
- InterPro:   IPR006151
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000445
- TIGRFAMs:   TIGR01035

Pfam domain/function: PF00745 GlutR_dimer; PF05201 GlutR_N; PF01488 Shikimate_DH; SSF69075 4pyrrol_synth_GluRdtase_C; SSF69742 GlutR

EC number: =1.2.1.70

Molecular weight: Translated: 48516; Mature: 48516

Theoretical pI: Translated: 7.55; Mature: 7.55

Prosite motif: PS00747 GLUTR

Important sites: ACT_SITE 50-50 BINDING 109-109 BINDING 120-120

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIVVVGLSHRTAPVEVREKLSIPDQAISESLKSLRAYSDILEVSILSTCNRLEIYGLVK
CEEEEEECCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEE
DKNIGISSIKEFLSDYSQVNCEVLTPHLFDFRQEEAVLHLMKVSAGLDSLVLGEGQILSQ
CCCCCHHHHHHHHHHHHHCCEEEECHHHHHHHHHHHHHHHHHHHCCCCHHEECCCHHHHH
VKKMMRLGQENQSTGPILNRLLSQSVSAGKKVRAETNLGTGAVSISSAAVELAQLKIGQD
HHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCEEHHHHHHHHHHHEECCC
NGIDGLVSLKGEKVLVVGAGRMSRLLITHLKSKGCNKLTLLNRNIDRAINLSGDFPDIEI
CCCCCEEEECCCEEEEEECCHHHHHHHHHHHCCCCCEEEEECCCCCCEEECCCCCCCHHH
ICKGLDELDKSITLSSLVFTSTASEKPFIDLERIKNISLNNKLKFIDIGVPRNISNDVKQ
HHHHHHHHHHHHHHHHHHEECCCCCCCCEEHHHHCCCCCCCCEEEEEECCCCCCCHHHHH
HDLIEAFDVDDLEEVVSRNQEFRQKIAKEAESLVKEERIIFLEWWASLEAVPVINKLRSD
HHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCEEEEEEHHCCCHHHHHHHHHHH
LELIRKEELQKALSRMGPDFSARERKVVEALTKGIINKILHTPVTKLRSPQSREERQASL
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHH
KIVEKLFSLMDDEQK
HHHHHHHHHHCCCCC
>Mature Secondary Structure
MQIVVVGLSHRTAPVEVREKLSIPDQAISESLKSLRAYSDILEVSILSTCNRLEIYGLVK
CEEEEEECCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEE
DKNIGISSIKEFLSDYSQVNCEVLTPHLFDFRQEEAVLHLMKVSAGLDSLVLGEGQILSQ
CCCCCHHHHHHHHHHHHHCCEEEECHHHHHHHHHHHHHHHHHHHCCCCHHEECCCHHHHH
VKKMMRLGQENQSTGPILNRLLSQSVSAGKKVRAETNLGTGAVSISSAAVELAQLKIGQD
HHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCEEHHHHHHHHHHHEECCC
NGIDGLVSLKGEKVLVVGAGRMSRLLITHLKSKGCNKLTLLNRNIDRAINLSGDFPDIEI
CCCCCEEEECCCEEEEEECCHHHHHHHHHHHCCCCCEEEEECCCCCCEEECCCCCCCHHH
ICKGLDELDKSITLSSLVFTSTASEKPFIDLERIKNISLNNKLKFIDIGVPRNISNDVKQ
HHHHHHHHHHHHHHHHHHEECCCCCCCCEEHHHHCCCCCCCCEEEEEECCCCCCCHHHHH
HDLIEAFDVDDLEEVVSRNQEFRQKIAKEAESLVKEERIIFLEWWASLEAVPVINKLRSD
HHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCEEEEEEHHCCCHHHHHHHHHHH
LELIRKEELQKALSRMGPDFSARERKVVEALTKGIINKILHTPVTKLRSPQSREERQASL
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHH
KIVEKLFSLMDDEQK
HHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA